FastQCFastQC Report
Fri 17 Jun 2016
SRR1525911_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525911_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2070
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT291.4009661835748792No Hit
CTCCATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG200.966183574879227No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG160.7729468599033816No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT130.6280193236714976No Hit
CTCCATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.43478260869565216No Hit
CTCCATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.43478260869565216No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC90.43478260869565216No Hit
CTCCATGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG70.33816425120772947No Hit
CTCCATGGGGCTCTCCATGGGTCACAGATCCTCTTCTGAG70.33816425120772947No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT50.24154589371980675No Hit
CTCCATGGGTGTTGGGTGCGTTGATTTAGATACGTTTATG50.24154589371980675No Hit
CTCCATGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT40.1932367149758454No Hit
CTCCATGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT40.1932367149758454No Hit
CTCCATGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT40.1932367149758454No Hit
CTCCATGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAG40.1932367149758454No Hit
CTCCATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1932367149758454No Hit
CTCCATGGGGGCTTTTCAAATATGCGCTATATTTCTTCAT40.1932367149758454No Hit
CTCCATGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT40.1932367149758454No Hit
CTCCATGGGGCTCTCCATGGGTCACAGATCCTCTTCTGAA40.1932367149758454No Hit
CTCCATGGGGGGGGTTGGTATGAGTCCAAGTGAGTCCTCT40.1932367149758454No Hit
CTCCATGGGGGGGCTGGTGAGCTGGCTCAGTGGGTAAGAG40.1932367149758454No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGTAAGAGC30.14492753623188406No Hit
CTCCATGGGTCACAGCTCCTCTTCTGAGATGAGTTTTTGT30.14492753623188406No Hit
CTCCATGGGACACAGATCCTCTTCTGAGATGAGTTTTTGT30.14492753623188406No Hit
CTCCATGGGAAGAAGTGGTATCAACGCAGAGTCGACTTTT30.14492753623188406No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA30.14492753623188406No Hit
CTCCATGGGATTGTTAAAAAATTTAAAGCACTTTAAATGG30.14492753623188406No Hit
CTCCATGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT30.14492753623188406No Hit
CTCCATGGGGCTCTCCATGGGAAGCAGTGGTATCAACGCA30.14492753623188406No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGA30.14492753623188406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG650.034.0000045
CTCTCCA251.4085582E-434.00000411
TGGGGGC251.4085582E-434.0000046
TGGGGCT251.4085582E-434.0000046
GGCTCTC251.4085582E-434.0000049
GGGGCTC251.4085582E-434.0000047
GCTCTCC251.4085582E-434.00000410
GGGCTCT251.4085582E-434.0000048
ATGGGGC355.460952E-734.05
ATGGGGA200.002191459834.05
TGGGGGA200.002191459834.06
TCTCCAT200.002191459834.012
CATGGGG1400.032.7857134
CTCCATG2250.030.977781
TCCATGG2250.030.977782
CCATGGG2200.030.9090923
CATGGGT304.116301E-428.3333324
CATGGGA406.3651554E-525.54
Produced by FastQC (version 0.11.5)