FastQCFastQC Report
Fri 17 Jun 2016
SRR1525910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72918
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG8011.098494198963219No Hit
TTAACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC6550.8982692887901479No Hit
TTAACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT6100.8365561315450233No Hit
TTAACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC2550.3497078910557064No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG2400.32913683864066484No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1670.22902438355412927No Hit
TTAACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1600.2194245590937766No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1470.2015963136674072No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1030.14125455991661867No Hit
TTAACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA1020.13988315642228258No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG970.13302613895060206No Hit
TTAACTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT860.11794070051290491No Hit
TTAACTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC810.1110836830412244No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG750.10285526207520776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA309.802279E-750.38958440
GCGCATT950.050.38958438
CGCTTCT251.9750638E-550.38958440
AGCCTTC204.0151554E-450.35461839
CGCATTG850.050.35461439
GTCTAAT150.008167850.35461439
CTCATAC150.008167850.35461439
CGCTTGC150.008167850.35461439
AGCGCTT150.00821274550.28482437
GAGCCGA150.00821274550.28482437
TGCTCGT354.969843E-850.28482437
ACCATAG204.056626E-450.2534
CATAGCC204.056626E-450.2536
CTCACTA204.070525E-450.21522545
TATATTC252.0243291E-550.18049614
CACGATT252.0243291E-550.18049647
ATATTCC252.0243291E-550.18049615
TAATCCG150.00828050650.18049642
CGACTCC150.00830318650.1458244
ACCTGGT2050.050.07660311