##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525910_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72918 Sequences flagged as poor quality 0 Sequence length 56 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.697907238267646 38.0 37.0 40.0 31.0 40.0 2 36.299308812638856 38.0 36.0 40.0 30.0 40.0 3 34.404303464165224 38.0 35.0 39.0 17.0 40.0 4 32.99433610356839 38.0 31.0 39.0 15.0 40.0 5 35.59713650950383 38.0 35.0 40.0 27.0 40.0 6 36.00289366137305 39.0 36.0 40.0 27.0 40.0 7 36.4809923475685 39.0 37.0 40.0 30.0 40.0 8 36.18121725774157 39.0 36.0 40.0 30.0 40.0 9 36.05296360295126 39.0 36.0 40.0 29.0 40.0 10 35.079678543020925 38.0 35.0 39.0 25.0 40.0 11 35.346937655997145 38.0 35.0 39.0 28.0 40.0 12 34.249074302641326 38.0 33.0 39.0 23.0 40.0 13 33.59867248141748 38.0 32.0 39.0 20.0 40.0 14 34.19218848569626 38.0 33.0 39.0 22.0 40.0 15 33.018129954195125 38.0 31.0 39.0 17.0 40.0 16 32.508036424476806 37.0 30.0 39.0 18.0 40.0 17 31.595751391974545 36.0 28.0 39.0 15.0 40.0 18 31.037384459255602 35.0 26.0 39.0 14.0 40.0 19 32.15986450533476 36.0 29.0 39.0 18.0 40.0 20 32.89142598535341 37.0 30.0 39.0 21.0 40.0 21 33.25968896568749 38.0 31.0 39.0 20.0 40.0 22 32.52667379796484 38.0 30.0 39.0 17.0 40.0 23 33.22567815902795 38.0 31.0 39.0 20.0 40.0 24 32.620038947859236 37.0 30.0 39.0 17.0 40.0 25 33.05887435201185 38.0 31.0 39.0 19.0 40.0 26 33.53285882772429 38.0 33.0 39.0 19.0 40.0 27 33.79095696535835 38.0 33.0 39.0 22.0 40.0 28 33.754025069255874 38.0 33.0 39.0 22.0 40.0 29 33.273060149757264 38.0 32.0 39.0 19.0 40.0 30 33.05157848542198 38.0 32.0 39.0 18.0 40.0 31 33.811349735319126 38.0 33.0 39.0 22.0 40.0 32 33.71787487314518 38.0 33.0 39.0 21.0 40.0 33 33.569009023834994 38.0 33.0 39.0 21.0 40.0 34 33.39996982912312 38.0 33.0 39.0 19.0 40.0 35 33.39001343975424 38.0 33.0 39.0 19.0 40.0 36 32.55342988013933 37.0 32.0 39.0 17.0 40.0 37 32.34231602622123 36.0 31.0 39.0 14.0 40.0 38 31.632271867028717 36.0 30.0 38.0 9.0 40.0 39 30.56519652212074 35.0 28.0 38.0 4.0 40.0 40 28.368907539976412 34.0 21.0 38.0 4.0 40.0 41 26.353026687512 31.0 16.0 38.0 4.0 39.0 42 27.272539016429413 32.0 20.0 38.0 4.0 39.0 43 27.456019089936643 33.0 21.0 38.0 4.0 39.0 44 26.42128143942511 31.0 17.0 38.0 4.0 39.0 45 27.436408019967637 33.0 22.0 38.0 4.0 39.0 46 25.389492306426398 30.0 16.0 36.0 4.0 39.0 47 24.4521791601525 29.0 10.0 36.0 4.0 39.0 48 25.108025453248853 30.0 15.0 36.0 4.0 39.0 49 24.065841081763075 28.0 9.0 36.0 4.0 39.0 50 24.816163361584245 29.0 12.0 36.0 4.0 39.0 51 25.13371184069777 30.0 4.0 36.0 4.0 39.0 52 24.994994377245675 30.0 4.0 36.0 4.0 39.0 53 24.869744096107958 30.0 4.0 36.0 4.0 39.0 54 24.434707479634657 30.0 4.0 36.0 4.0 39.0 55 24.2099755890178 30.0 4.0 36.0 4.0 39.0 56 23.063111988809347 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 3.1526418867637602 1 2 3.705696158851154 1 3 6.0611398760545825 1 4 7.783109084676401 1 5 4.336755009072142 1 6 4.128022491060939 1 7 3.569356201797177 1 8 3.8459579655889016 1 9 4.0778678614328285 1 10 4.895331657080959 1 11 0.04341827850057456 1 12 -8.397270622370115 1 13 -19.157491366009076 1 14 -6.733582590039571 1 15 0.13582060396855056 1 16 -0.8785325654810805 1 17 -6.485513220732162 1 18 -4.424061551873471 1 19 -0.05363939734374412 1 20 -0.6281944942393523 1 21 5.658827275718195 1 22 6.760363531537529 1 23 5.800250625625402 1 24 6.423290469941108 1 25 5.694771637123811 1 26 6.19824599420927 1 27 5.6758283314381615 1 28 5.81077536348808 1 29 6.475209145950949 1 30 6.008751989780588 1 31 4.661065572021094 1 32 4.898237157012044 1 33 5.423035050147444 1 34 4.700526376721388 1 35 4.696336606520418 1 36 3.852468257629809 1 37 4.0563459231171315 1 38 4.86026464283583 1 39 4.805387928289072 1 40 -8.246722512575776 1 41 0.8597282817615302 1 42 -0.9375041593949085 1 43 -8.854468141895614 1 44 -14.842944051209152 1 45 -2.798003785258139 1 46 -5.148969413780879 1 47 -11.031509200465957 1 48 -4.9855252381145085 1 49 -13.331823399442445 1 50 -3.6668500508426156 1 51 2.6852034346338236 1 52 2.116014653670806 1 53 2.687098829315378 1 54 2.7284914321720386 1 55 2.4475494477290596 1 56 5.232390866115992 2 1 3.685633089109807 2 2 4.140886774686926 2 3 6.358647207432881 2 4 8.269032838342088 2 5 4.589980815523759 2 6 4.626849470533081 2 7 4.026834207662276 2 8 4.330855326292713 2 9 4.710859063778869 2 10 5.163073592564828 2 11 3.3390194515211036 2 12 -0.10973396547861825 2 13 1.6559983700613046 2 14 4.613484858640781 2 15 5.4182252960506645 2 16 4.932757757099566 2 17 7.34439880272825 2 18 4.827404723786351 2 19 6.124952978022822 2 20 5.728257118663873 2 21 7.169677715600898 2 22 7.753618663502337 2 23 6.823857663748569 2 24 7.5196247807915455 2 25 6.7370003761267405 2 26 6.645899953153556 2 27 6.2837462200012055 2 28 5.9923002901743025 2 29 6.513478940672357 2 30 6.914470464853903 2 31 5.228660879938978 2 32 5.43636032416747 2 33 5.725527718769147 2 34 5.605365086398802 2 35 5.558213439364991 2 36 6.586192597805763 2 37 6.4635306738502685 2 38 7.293695727879822 2 39 7.983393793391713 2 40 5.821166050474076 2 41 11.29946435214276 2 42 9.533903465238524 2 43 9.865766462209958 2 44 -1.6029147256959568 2 45 9.040119381897291 2 46 8.150737331087157 2 47 4.70485443589768 2 48 8.068287078601035 2 49 0.3214024070091668 2 50 7.409982793732166 2 51 9.910130120850834 2 52 9.740501457189868 2 53 9.880207333714203 2 54 10.12306621223069 2 55 9.916611031306775 2 56 11.559956848520685 3 1 3.6024851469518495 3 2 4.172310578913844 3 3 6.469603825897842 3 4 8.146118489065113 3 5 4.471237767692834 3 6 4.485859481656554 3 7 4.228447110888084 3 8 4.432635081576365 3 9 4.567366294034706 3 10 5.367745450184401 3 11 4.256427682889289 3 12 4.686428437191012 3 13 5.833417724900016 3 14 4.808423679552902 3 15 1.1050996008337535 3 16 6.754226055208576 3 17 7.633609036321133 3 18 8.59631462367512 3 19 7.312661543095128 3 20 6.378702057484784 3 21 7.394093733398449 3 22 7.501115882634707 3 23 6.759341534716313 3 24 7.144763824173083 3 25 6.985053768785253 3 26 6.0621958374694636 3 27 5.21172174836606 3 28 5.993690723989648 3 29 6.508751465700165 3 30 6.3830466606269844 3 31 4.45557967860416 3 32 4.617672893689157 3 33 5.2266400658214245 3 34 5.3022505146524175 3 35 5.282073283636407 3 36 6.3056030538680545 3 37 6.821706424684525 3 38 7.126565583274704 3 39 8.225607364025748 3 40 9.770832346358393 3 41 12.115370914990368 3 42 11.242746624304147 3 43 10.899136873778364 3 44 12.513325541267335 3 45 10.445569882453462 3 46 12.617645006281816 3 47 12.851406160035609 3 48 12.576073507966996 3 49 13.978521428143761 3 50 11.942240858248294 3 51 12.248839798270186 3 52 11.887331268090868 3 53 11.71891701113356 3 54 12.22206509988364 3 55 12.352094902274516 3 56 11.532704345739816 4 1 3.4990368710897854 4 2 4.060241613396606 4 3 6.29719003279439 4 4 8.077152971823732 4 5 4.445375698727318 4 6 4.546204309242761 4 7 3.9181022833018773 4 8 4.165393702266016 4 9 4.300124914724364 4 10 4.962573036391298 4 11 4.6529794070272175 4 12 5.41918705788067 4 13 5.9885901386931195 4 14 5.868768507139109 4 15 6.001651324971689 4 16 7.07319157244995 4 17 8.495678001838371 4 18 9.122176692640636 4 19 7.821282232750303 4 20 7.137322747139958 4 21 7.238921319605346 4 22 8.08732277918643 4 23 7.276582914026655 4 24 7.894763824173083 4 25 7.260915837750769 4 26 6.294954458159115 4 27 5.754825196641917 4 28 5.493690723989648 4 29 6.55185491397603 4 30 6.87442597097181 4 31 5.395234851017953 4 32 5.609052204033983 4 33 5.640433169269702 4 34 5.267767756031727 4 35 5.506211214670891 4 36 6.7280168469715065 4 37 7.157913321236254 4 38 7.773117307412633 4 39 7.975607364025748 4 40 3.6501426911859802 4 41 12.839508846024852 4 42 10.570332831200702 4 43 11.2956885979163 4 44 12.202980713681129 4 45 10.264535399694843 4 46 13.41074845455768 4 47 13.342785470380441 4 48 12.395039025208376 4 49 12.547486945385142 4 50 13.295689134110365 4 51 12.481598418959845 4 52 12.645951957746043 4 53 11.744779080099075 4 54 12.747927168849156 4 55 12.188301798826238 4 56 10.662014690567403 5 1 3.71627825040013 5 2 4.251545961222696 5 3 6.485645804908337 5 4 8.370856120249524 5 5 4.562841965593883 5 6 4.35489996141667 5 7 4.065403632627216 5 8 4.033309744245024 5 9 4.289330312025712 5 10 5.408375135341828 5 11 4.613774009725873 5 12 4.838302500159529 5 13 5.494287290117406 5 14 5.272741520632366 5 15 6.404200050608871 5 16 7.147554391040657 5 17 7.85954606780539 5 18 8.224950305834035 5 19 7.680352697517915 5 20 7.119631592717173 5 21 7.397167196666814 5 22 7.636648116517769 5 23 6.69629805645544 5 24 7.531195608281031 5 25 6.453269660839229 5 26 6.29982702187726 5 27 5.5509271456674085 5 28 4.668878130286505 5 29 6.122244719073478 5 30 6.297289539187698 5 31 4.623120907989467 5 32 5.220371544363818 5 33 4.737659556076299 5 34 4.561395941938773 5 35 5.487245697429508 5 36 6.440610550119928 5 37 6.480851851970886 5 38 7.094256737697492 5 39 7.68760136702425 5 40 9.256439542760194 5 41 12.668819190852442 5 42 10.798068963134732 5 43 11.538417233598455 5 44 12.263775316379778 5 45 10.828478428180595 5 46 12.722967345112405 5 47 13.05702834894116 5 48 12.948862113664148 5 49 13.450785296209727 5 50 12.229197379987422 5 51 11.374926754791925 5 52 11.33973006869057 5 53 11.073264837220517 5 54 11.194104080393384 5 55 11.219486206622342 5 56 9.67033553014761 6 1 3.525369159491035 6 2 4.3147870679420635 6 3 6.552048966963675 6 4 8.57401817558549 6 5 4.564027736344869 6 6 4.791658854697303 6 7 4.096628929069901 6 8 4.291412511043447 6 9 4.559686043251006 6 10 5.258968020717319 6 11 4.725236460318754 6 12 5.47545665035716 6 13 6.160690452172744 6 14 5.839144682687703 6 15 6.872184240332189 6 16 7.339649252700738 6 17 8.359941324722385 6 18 9.294120266308347 6 19 7.809996966292619 6 20 6.917260051215191 6 21 7.295190912081836 6 22 7.387636258810261 6 23 6.836614261989041 6 24 7.3232904699411066 6 25 6.831135273487444 6 26 5.616427812391091 6 27 5.466737422347251 6 28 5.7653208180335405 6 29 5.75702732776913 6 30 5.845115626144224 6 31 4.370156481111998 6 32 4.307328066102951 6 33 4.71394414105654 6 34 4.0005263767213854 6 35 4.932700242884053 6 36 5.997922803084357 6 37 6.510891377662585 6 38 6.105719188290379 6 39 8.187206110107255 6 40 4.935095669242404 6 41 12.159728281761527 6 42 10.580677658786911 6 43 10.663713676286203 6 44 11.43887413060903 6 45 11.038359851105497 6 46 12.760121495310035 6 47 13.723036254079496 6 48 12.03265658006731 6 49 13.259085691466648 6 50 12.896786312793747 6 51 13.148839798270185 6 52 12.66146919912535 6 53 12.523462465679014 6 54 12.128491432172037 6 55 11.938458538638152 6 56 12.268754502479627 7 1 3.4041287176898436 7 2 4.144353762929313 7 3 6.652813622868514 7 4 8.291945108465693 7 5 4.441427906268409 7 6 4.5088211316726685 7 7 4.109288232383413 7 8 4.242899341969526 7 9 4.438700486751429 7 10 5.489724180445442 7 11 4.5821608443459425 7 12 5.211565401419179 7 13 5.581081276301887 7 14 5.6735541984056255 7 15 6.40013666174255 7 16 7.755112294332001 7 17 8.548046677313714 7 18 9.247136409044117 7 19 7.504814298493127 7 20 6.723207374919529 7 21 7.489243588377498 7 22 7.466310855241865 7 23 6.831771441258368 7 24 7.387351642413492 7 25 6.7290112293073285 7 26 6.697226453002816 7 27 5.941418815720233 7 28 6.375176383029288 7 29 6.443008636180345 7 30 7.020816560723663 7 31 5.693011196490083 7 32 5.840038346476785 7 33 5.54351083604379 7 34 6.145386189805496 7 35 6.098460650700538 7 36 6.994694255930575 7 37 7.141477613856299 7 38 7.552363198485793 7 39 9.208446551908445 7 40 8.421331862955231 7 41 12.038912648796366 7 42 11.156378593366348 7 43 11.746381475776435 7 44 12.660199534177423 7 45 11.6634235724309 7 46 14.169127442633734 7 47 14.410461912533194 7 48 12.770804413542244 7 49 14.93920463794073 7 50 14.353455811519321 7 51 12.935755686120654 7 52 13.842777610340306 7 53 13.576393646647578 7 54 13.932909444066684 7 55 13.695552846199746 7 56 12.546239634170366 8 1 3.1483537220982427 8 2 3.975335953019247 8 3 6.105908315162644 8 4 7.942800336820479 8 5 4.295245575109881 8 6 4.178279780940876 8 7 3.9595791863597825 8 8 3.902218686000566 8 9 4.296221206201267 8 10 5.157595808024354 8 11 3.9540529268710713 8 12 5.419024403358868 8 13 6.012663007918881 8 14 5.553381389377236 8 15 6.085906367261863 8 16 7.295052340179296 8 17 7.7546240005371345 8 18 9.001324382946422 8 19 7.75459387881406 8 20 6.8416168265153665 8 21 6.795362438668455 8 22 6.660192004950908 8 23 7.094247195093672 8 24 7.327750161193251 8 25 6.4232450505028815 8 26 6.121402083403094 8 27 5.448384077578808 8 28 5.403742773436623 8 29 5.674008459844607 8 30 5.952662795955543 8 31 4.943055280425895 8 32 4.590346934027476 8 33 5.0914741582091985 8 34 4.6703376974761035 8 35 4.7815853200710166 8 36 5.515590041506314 8 37 6.133189833923307 8 38 6.360950749182315 8 39 7.28497626448118 8 40 1.5109104205288553 8 41 11.355611643682625 8 42 10.135394639918985 8 43 9.608310588807644 8 44 10.692733478808002 8 45 9.835615425719567 8 46 12.448972267179673 8 47 11.846363869859942 8 48 12.120478192417224 8 49 12.526667166595292 8 50 11.67808991485207 8 51 12.145066213364526 8 52 11.710525802898935 8 53 12.544388709246764 8 54 12.173431397866725 8 55 12.583226977746214 8 56 11.535306818088547 9 1 3.3959078800297533 9 2 3.9769082800632702 9 3 6.3469984619131665 9 4 8.292031643598957 9 5 3.992310564627701 9 6 4.379537642576096 9 7 4.0432619257029 9 8 4.160604430235367 9 9 4.263726447291411 9 10 4.991627953377254 9 11 4.319007504089797 9 12 5.378318603219107 9 13 5.70378809527039 9 14 5.763723807266828 9 15 6.4215108396587866 9 16 7.521467434518918 9 17 8.076776341557398 9 18 9.080988953177034 9 19 7.120771377067033 9 20 6.523657357612503 9 21 6.892497309388233 9 22 7.183427504601504 9 23 6.611193386568168 9 24 7.121775318425954 9 25 7.00133729368947 9 26 5.938313334276611 9 27 5.675323280933107 9 28 5.263805666518383 9 29 5.7718421425839495 9 30 6.402523033551628 9 31 5.197597558553078 9 32 5.594365103139989 9 33 4.533472760585159 9 34 5.028304154499164 9 35 5.122430882614694 9 36 6.603815058976615 9 37 6.57553784230905 9 38 6.545786528357716 9 39 7.596297019198161 9 40 5.926004760151496 9 41 10.654004376037623 9 42 10.362159140268389 9 43 10.539302901875427 9 44 11.304193995928898 9 45 9.92960564235129 9 46 11.95002048520902 9 47 12.010409991453237 9 48 12.047976445387178 9 49 12.890398822779776 9 50 11.720018636026072 9 51 11.36921016864056 9 52 11.509617347273498 9 53 10.987435529652078 9 54 11.835730489411095 9 55 11.370613420793035 9 56 10.195858879584005 10 1 3.3062782504001262 10 2 3.850241613396605 10 3 5.624776239690945 10 4 7.871290902858217 10 5 3.776755009072147 10 6 4.236204309242758 10 7 3.6884471108880845 10 8 3.775048874679811 10 9 3.9215042250691923 10 10 3.915331657080955 10 11 4.251600096682395 10 12 5.010911195811701 10 13 5.153417724900017 10 14 5.231871955414974 10 15 6.228547876695828 10 16 7.321467434518915 10 17 8.010850415631474 10 18 8.793211175399257 10 19 7.098178784474438 10 20 6.711805505760648 10 21 5.840645457536375 10 22 7.1249089860829855 10 23 6.319341534716315 10 24 6.945108651759291 10 25 6.040226182578355 10 26 5.454609630572907 10 27 4.943101058710887 10 28 4.80713899985172 10 29 5.087027327769135 10 30 5.855115626144222 10 31 4.17833829929382 10 32 4.377328066102951 10 33 3.6593986865110857 10 34 3.7105263767213863 10 35 3.6872456974295105 10 36 5.689740984902535 10 37 6.2199822867534955 10 38 6.942082824654015 10 39 6.836297019198163 10 40 9.176004760151496 10 41 10.60881919085244 10 42 11.360677658786905 10 43 10.32189549446802 10 44 10.591601403336306 10 45 10.301087123832772 10 46 10.405576040764576 10 47 11.88485443589768 10 48 11.39538385279458 10 49 12.148176600557552 10 50 10.702240858248292 10 51 10.068839798270186 10 52 10.021469199125349 10 53 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4.402554017468724 115 4 4.356846458413774 115 5 3.2700883424054794 115 6 3.462870975909425 115 7 2.6728915553325265 115 8 3.1606044302353666 115 9 3.277615336180304 115 10 4.209220545969842 115 11 1.0782667633490561 115 12 -1.36242213752163 115 13 -2.374915608433316 115 14 -6.7131280445850265 115 15 -6.194229901081954 115 16 2.0631341011855824 115 17 3.3869615267425885 115 18 3.5948778420659195 115 19 2.194845451141102 115 20 2.380138839093984 115 21 4.915645457536378 115 22 5.80379787497187 115 23 5.31489709027187 115 24 6.121775318425954 115 25 5.62633729368947 115 26 3.942942963906244 115 27 3.8003232809331067 115 28 4.19436122207394 115 29 3.818138438880247 115 30 5.217337848366444 115 31 4.243893854849375 115 32 4.293439177214065 115 33 3.709398686511083 115 34 3.9310819322769426 115 35 4.215023475207289 115 36 5.557518762680317 115 37 5.436648953420157 115 38 6.008749491320678 115 39 7.221297019198161 115 40 8.46767142681816 115 41 9.885485857519104 115 42 9.311233214342465 115 43 6.284673272245801 115 44 -1.2606208188859185 115 45 7.966642679388329 115 46 2.686131596320134 115 47 -7.503478897435654 115 48 3.0526060750168043 115 49 -2.8873789549980025 115 50 2.4422408582482937 115 51 4.179395353825743 115 52 4.069802532458684 115 53 4.135583677800227 115 54 4.79869345237406 115 55 4.7687615689411835 115 56 7.871784805509932 116 1 2.0825433106410927 116 2 3.1807235411074473 116 3 3.8898364806548003 116 4 4.939845119725685 116 5 3.2982007922046748 116 6 3.5221079237005952 116 7 2.773627833779649 116 8 2.788783814438844 116 9 3.613793381695693 116 10 3.29774129563517 116 11 3.0893109400558885 116 12 3.2932003524382054 116 13 4.231008086345803 116 14 3.8094623168607598 116 15 -2.664946099207789 116 16 3.256407193555063 116 17 4.831573307197736 116 18 5.158632862146241 116 19 4.437817338691307 116 20 3.424817553953421 116 21 4.920163529825537 116 22 5.264306576444433 116 23 4.551510209415106 116 24 5.693060458988207 116 25 4.610105700650646 116 26 4.216537341416281 116 27 4.118763709313292 116 28 4.720632975755333 116 29 4.451003231383588 116 30 4.46198309602374 116 31 3.3931575764022526 116 32 3.8133521624884956 116 33 3.266627602173731 116 34 3.70534565382982 116 35 3.9161613600801104 116 36 5.285765081288083 116 37 6.176608792777589 116 38 6.613167162003414 116 39 6.650513886668044 116 40 9.371787892681613 116 41 8.75797581735846 116 42 5.368629466015825 116 43 1.3698473016969395 116 44 3.0670230900832927 116 45 6.013496762386989 116 46 1.553768811848915 116 47 -5.216832311090272 116 48 1.6571910817102449 116 49 -1.9944740018520832 116 50 1.5085059184892593 116 51 5.447634978993079 116 52 5.500023415992821 116 53 5.02915797498898 116 54 5.666498003913553 116 55 5.409323817937167 116 56 8.693069946072182 117 1 2.5662782504001243 117 2 3.0769082800632717 117 3 4.491442906357612 117 4 5.9846242361915465 117 5 3.803421675738811 117 6 3.596204309242758 117 7 3.028447110888081 117 8 3.4717155413464766 117 9 3.5081708917358583 117 10 4.445331657080956 117 11 1.0449334300157247 117 12 -3.945755470854966 117 13 -8.149915608433318 117 14 -0.4547947112516937 117 15 -6.82478545663751 117 16 -7.045199232147748 117 17 0.6841837489648093 117 18 -1.780122157934077 117 19 0.6948454511411057 117 20 -0.07819449423935154 117 21 4.373978790869714 117 22 5.68157565274965 117 23 4.05934153471631 117 24 4.538441985092625 117 25 5.223559515911692 117 26 5.184609630572911 117 27 4.353101058710884 117 28 4.647138999851716 117 29 4.8236939944358 117 30 4.845115626144224 117 31 3.2216716326271495 117 32 3.3739947327696207 117 33 2.8093986865110843 117 34 2.3171930433880554 117 35 3.403912364096179 117 36 3.9630743182358685 117 37 3.9033156200868255 117 38 4.392082824654011 117 39 5.712963685864828 117 40 7.392671426818165 117 41 6.76881919085244 117 42 4.580677658786907 117 43 -5.184771172198644 117 44 -4.1383985966636985 117 45 3.4777537904994382 117 46 -1.8444239592354243 117 47 -3.3784788974356523 117 48 -1.3446161472054179 117 49 -2.498490066109113 117 50 0.425574191581628 117 51 3.8155064649368526 117 52 3.6448025324586872 117 53 3.01891701113356 117 54 3.9709156745962844 117 55 4.152094902274516 117 56 6.688451472176599 118 1 1.9990368710897854 118 2 2.5774829927069476 118 3 4.124776239690945 118 4 5.404739178720288 118 5 2.367789491830763 118 6 2.2703422402772446 118 7 2.685343662612219 118 8 2.6309109436453255 118 9 3.1104697423105705 118 10 3.091883381218885 118 11 0.265048372544463 118 12 -6.072192252464159 118 13 -5.252789171651706 118 14 -7.932955630791923 118 15 -10.791452123304175 118 16 -9.89232566892936 118 17 -1.4870806188512837 118 18 -6.869202617704193 118 19 -1.195959146560046 118 20 -0.8971600114807323 118 21 3.6958178713294814 118 22 5.276977951600223 118 23 3.836927741612868 118 24 4.153384513828257 118 25 4.1229848032680145 118 26 4.389782044366012 118 27 4.547928644917782 118 28 4.519552792955164 118 29 4.560475603631204 118 30 3.8658052813166357 118 31 3.6193727820524373 118 32 3.565948755758125 118 33 3.166295238235218 118 34 3.9315608594800047 118 35 4.11828018018813 118 36 4.365947881454261 118 37 4.726878838477628 118 38 5.824841445343672 118 39 5.9583659847154 118 40 8.874280622220464 118 41 6.1067502253351975 118 42 6.484125934648976 118 43 0.0025851496404349916 118 44 -1.3918468725257647 118 45 4.773156089350014 118 46 -0.45994120061473254 118 47 -1.2089386675505978 118 48 -0.6566851127226592 118 49 1.0474869453851419 118 50 2.7008615479034646 118 51 7.162632901718464 118 52 7.171814026711559 118 53 5.796503218030111 118 54 6.868616824021569 118 55 6.067612143653825 118 56 8.325807794015677 119 1 2.298886946052299 119 2 2.8573430626719656 119 3 5.247964645488047 119 4 6.779551772423435 119 5 3.0526970380576515 119 6 3.3288130048949327 119 7 2.7030847920475054 119 8 2.8478024978682157 119 9 3.106721616373541 119 10 3.8547519469360267 119 11 1.1760928503055794 119 12 -6.605175760710036 119 13 -2.354988072201433 119 14 -5.675084566324156 119 15 -6.363915891420117 119 16 -6.2611412611332575 119 17 0.46244461853002505 119 18 -4.2040352014123386 119 19 0.02817878447443789 119 20 -0.5528321753987733 119 21 4.605862848840729 119 22 5.538097391880086 119 23 4.835428491238055 119 24 3.676123144512914 119 25 4.937327631853716 119 26 5.5172183262250805 119 27 4.898753232623932 119 28 4.738443347677805 119 29 5.275867907479281 119 30 5.303086640636973 119 31 4.130367284801068 119 32 3.5392121240739662 119 33 4.618818976366153 119 34 4.16719304338805 119 35 5.093042798878784 119 36 5.968146782003991 119 37 5.628677938927407 119 38 6.349329201465608 119 39 7.241224555430044 119 40 8.810062731165988 119 41 6.106500350272729 119 42 3.9603878037144433 119 43 -4.907959577995747 119 44 -1.4405725097071738 119 45 4.88934799339799 119 46 -1.8567427998151338 119 47 -2.79514556410232 119 48 -5.265630639959042 119 49 -5.027475573355488 119 50 -0.27515044609953065 119 51 4.4879702330527955 119 52 4.731034416516657 119 53 4.345728605336458 119 54 4.278162051407875 119 55 4.5006456269121955 119 56 7.531205095365003 120 1 2.0380173808349085 120 2 2.66893726557052 120 3 4.682747254183695 120 4 6.098392352133576 120 5 3.0816825453040266 120 6 3.140407207793487 120 7 2.616128270308373 120 8 2.6304111935203878 120 9 2.671939007677885 120 10 3.275041802008495 120 11 -0.9398491786799283 120 12 -6.721117789695544 120 13 -6.8332489417666515 120 14 -8.921461377918359 120 15 -2.5088434276519997 120 16 -7.59447459446659 120 17 -0.21871480175983038 120 18 -5.363455491267413 120 19 -1.2471835343661404 120 20 -1.1615278275726837 120 21 3.82325415318855 120 22 5.900416232459797 120 23 4.328182114426461 120 24 4.603659376396969 120 25 4.183704443447919 120 26 5.082435717529432 120 27 3.956724247116682 120 28 4.463081028837227 120 29 4.97152008139232 120 30 5.15815910440509 120 31 3.9564542413228025 120 32 3.292835312479763 120 33 4.039108831438622 120 34 4.659946666576456 120 35 3.9336225090237136 120 36 5.373943883453265 120 37 5.237373591101321 120 38 6.13193789711778 120 39 6.415137598908309 120 40 8.346294615223957 120 41 -5.197847475814228 120 42 -1.9961339354159904 120 43 -5.313756679445021 120 44 -14.121731929997031 120 45 -2.7773186732686774 120 46 -8.175583379525278 120 47 -5.461812230768988 120 48 -4.294616147205417 120 49 -14.868055283500416 120 50 -3.420077982331417 120 51 1.951738348994823 120 52 1.3107445614441922 120 53 1.8819604893944302 120 54 1.9738142253209219 120 55 1.6310804095208908 120 56 5.357292051886741 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1696.0 5 58.0 6 159.0 7 207.0 8 203.0 9 336.0 10 346.0 11 164.0 12 212.0 13 165.0 14 182.0 15 281.0 16 307.0 17 362.0 18 475.0 19 603.0 20 818.0 21 1215.0 22 1382.0 23 1735.0 24 1911.0 25 2124.0 26 2296.0 27 2416.0 28 2513.0 29 2696.0 30 2921.0 31 3209.0 32 3691.0 33 4188.0 34 4620.0 35 4851.0 36 5530.0 37 6168.0 38 6440.0 39 6433.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 100.0 0.0 7 99.55429386434076 0.3661647329877396 0.05211333278477193 0.027428069886722074 8 99.54606544337476 0.3154228036973038 0.08502701664883842 0.05348473627910803 9 98.15820510710661 0.6157601689569105 0.7570147288735292 0.46901999506294745 10 49.81211772127595 22.929866425299654 16.567925615074465 10.690090238349928 11 32.404893167667794 24.930744123536027 18.079212265832854 24.58515044296333 12 29.13546723717052 21.55023450999753 20.795962588112673 28.518335664719274 13 22.54587344688554 15.969993691543927 23.858306590965196 37.62582627060534 14 21.78748731451768 25.905812008008994 27.615952165446117 24.69074851202721 15 32.37560834378834 18.91552810444901 24.06215102091462 24.646712530848028 16 30.320827730054617 26.858687597771496 22.228778439498313 20.591706232675577 17 25.85145051779713 20.041149441053427 32.70008915712228 21.407310884027158 18 24.15590114923613 19.20239172769412 25.982610603691818 30.659096519377933 19 27.681352304378247 21.54823484214426 21.328704910610156 29.441707942867335 20 25.210811107754004 24.101134397231302 29.083692696459472 21.60436179855522 21 23.899776458164762 20.510991949751087 26.822277383874816 28.766954208209334 22 21.42680819550728 19.85792259798678 33.21264982583176 25.50261938067418 23 22.622672042568365 21.203269425930497 30.697495817219345 25.476562714281798 24 23.658008064294922 21.059055874043395 25.147433963299232 30.135502098362455 25 26.275870581942367 21.644196348973406 28.104126949294344 23.97580611978988 26 31.24622646687524 21.964707173829517 21.43229595477249 25.35677040452275 27 23.071750109793587 27.138230127360565 20.962340799297323 28.827678963548532 28 25.97473840119588 30.233004649121604 20.405392432491738 23.386864517190777 29 32.783845044302495 24.966000412116216 20.862696613778418 21.38745792980287 30 29.279872660409463 20.93007300071354 28.5841703715901 21.205883967286898 31 29.79508421572393 27.784330937620016 21.978383716464585 20.442201130191474 32 34.52447907378702 24.336410650351855 20.8425355629021 20.296574712959025 33 30.196218435470364 24.90422508135719 21.56343114504236 23.33612533813009 34 24.053259316258107 24.09585577662966 32.08200505661207 19.768879850500166 35 21.738592973106613 24.467763645853363 35.19215449276159 18.60148888827844 36 26.922865845292648 27.201382981642563 25.388963586971435 20.486787586093353 37 28.914653076363038 23.4144264186711 28.208580023902087 19.46234048106378 38 31.15772688532703 24.07142660764853 24.350479751464 20.420366755560444 39 27.844919932979924 23.557282940093938 22.020490565001236 26.577306561924907 40 23.901250968670432 17.876120890069743 25.575666998782243 32.64696114247758 41 19.361415870285864 16.50276710542741 23.654243588498826 40.4815734357879 42 23.239271906773247 26.597691344606027 24.426619704745264 25.73641704387546 43 18.552913983820375 22.428595014033352 29.702548016069564 29.315942986076713 44 20.608056891633016 23.809589437561158 27.416309486073402 28.166044184732424 45 26.17744906656402 22.873993016414175 28.39350031618597 22.55505760083583 46 21.237890797005722 26.631990312637605 24.406924262439453 27.72319462791722 47 18.17053836079134 24.71082925484249 24.12490521817054 32.99372716619563 48 24.595158368556845 24.01191195037602 25.94554536970961 25.447384311357524 49 21.054731021581546 25.489333021554035 24.056065253572854 29.399870703291565 50 23.012972022206345 25.203374924421478 25.691199912054085 26.09245314131809 51 20.60280759702725 27.770437654830722 26.340489953206713 25.286264794935313 52 20.549432427232496 28.353901547426 28.25770277327323 22.83896325206827 53 20.854312835328106 27.251341312422618 29.562525794469668 22.331820057779613 54 20.942005062176737 24.84180697700011 28.8447782546495 25.371409706173655 55 20.14228117732858 23.953875579651314 29.639618565354397 26.26422467766571 56 23.085701714584278 22.396843074976967 29.321178622007725 25.196276588431026 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 0.5 4 2.0 5 4.0 6 24.5 7 45.0 8 82.5 9 120.0 10 120.5 11 121.0 12 136.5 13 130.0 14 108.0 15 122.0 16 136.0 17 140.0 18 144.0 19 157.0 20 170.0 21 182.5 22 249.5 23 304.0 24 326.0 25 348.0 26 377.0 27 406.0 28 467.5 29 642.0 30 755.0 31 917.0 32 1079.0 33 1116.5 34 1154.0 35 1237.5 36 1321.0 37 1471.0 38 1780.0 39 1939.0 40 2482.5 41 3026.0 42 4247.5 43 5469.0 44 5728.0 45 5987.0 46 5248.5 47 4369.0 48 4228.0 49 4256.0 50 4284.0 51 4423.0 52 4562.0 53 4586.0 54 4662.0 55 4714.0 56 4855.0 57 4996.0 58 4677.5 59 4359.0 60 3843.0 61 3327.0 62 2990.5 63 2223.0 64 1792.0 65 1543.5 66 1295.0 67 1108.5 68 922.0 69 767.5 70 613.0 71 521.0 72 325.0 73 221.0 74 177.0 75 133.0 76 116.0 77 99.0 78 63.5 79 16.0 80 4.0 81 2.5 82 1.0 83 2.0 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5279903453193999 16 0.06034175375078856 17 0.017828245426369346 18 0.0 19 0.047999122301763626 20 0.14262596341095476 21 0.0013714034943361037 22 0.0 23 0.0 24 0.005485613977344415 25 0.009599824460352725 26 0.057598946762116354 27 0.0740557886941496 28 0.0013714034943361037 29 0.16868262980334073 30 0.057598946762116354 31 0.013714034943361037 32 0.026056666392385967 33 0.12479771798458543 34 0.19473929619572672 35 0.1535971913656436 36 0.04388491181875532 37 0.16593982281466851 38 0.23588140102580982 39 0.14262596341095476 40 0.8968978852958118 41 1.125922268849941 42 0.32227982116898435 43 0.3209084176746482 44 0.49233385446666117 45 0.24136701500315424 46 0.33736525960668146 47 0.5252475383307277 48 0.06857017471680518 49 0.29759455827093445 50 0.20022491017307112 51 0.3538221015387147 52 0.20845333113908773 53 0.3126799967086316 54 0.304451575742615 55 0.3359938561123454 56 0.25919526042952357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 72918.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.79933624070874 #Duplication Level Percentage of deduplicated Percentage of total 1 88.4433129541189 65.27057790943252 2 6.994592384739747 10.323925505362187 3 1.9660676788136697 4.352834691022793 4 0.8473788861427536 2.501439973669053 5 0.499879211342984 1.844537699882059 6 0.28989277683831044 1.283633670698593 7 0.19697842528756992 1.017581392797389 8 0.13565495326408117 0.8008996406922845 9 0.13193837920205154 0.8763268328807701 >10 0.44784717447456934 5.916234674565951 >50 0.027874305465222158 1.3686606873474314 >100 0.013008009217103673 1.6100277023505856 >500 0.005574861093044432 2.8333196192983903 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 801 1.098494198963219 No Hit TTAACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 655 0.8982692887901479 No Hit TTAACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 610 0.8365561315450233 No Hit TTAACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 255 0.3497078910557064 No Hit TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 240 0.32913683864066484 No Hit TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 167 0.22902438355412927 No Hit TTAACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 160 0.2194245590937766 No Hit TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 147 0.2015963136674072 No Hit TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 103 0.14125455991661867 No Hit TTAACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 102 0.13988315642228258 No Hit TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 97 0.13302613895060206 No Hit TTAACTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 86 0.11794070051290491 No Hit TTAACTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC 81 0.1110836830412244 No Hit TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 75 0.10285526207520776 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.002742806988672207 0.0 0.0 0.0 0.0 11 0.010971227954688828 0.0 0.0 0.0 0.0 12 0.013714034943361035 0.0 0.0 0.0 0.0 13 0.016456841932033242 0.0 0.0 0.0 0.0 14 0.032913683864066484 0.0 0.0 0.0 0.0 15 0.03565649085273869 0.0 0.0 0.0 0.0 16 0.0383992978414109 0.0 0.0 0.0 0.0 17 0.041142104830083105 0.0 0.0 0.0 0.0 18 0.04388491181875531 0.0 0.0 0.0 0.0 19 0.047999122301763626 0.0 0.0 0.0 0.0 20 0.05348473627910804 0.0 0.0 0.0 0.0 21 0.05622754326778025 0.0 0.0 0.0 0.0 22 0.05759894676211635 0.0 0.0 0.0 0.0 23 0.06171315724512466 0.0 0.0 0.0 0.0 24 0.06308456073946077 0.0 0.0 0.0 0.0 25 0.06445596423379686 0.0 0.0 0.0 0.0 26 0.06719877122246908 0.0 0.0 0.0 0.0 27 0.0767985956828218 0.0 0.0 0.0 0.0 28 0.08502701664883842 0.0 0.0 0.0 0.0 29 0.08776982363751062 0.0 0.0 0.0 0.0 30 0.08914122713184673 0.0 0.0 0.0 0.0 31 0.09736964809786336 0.0 0.0 0.0 0.0 32 0.10148385858087167 0.0 0.0 0.0 0.0 33 0.10696947255821608 0.0 0.0 0.0 0.0 34 0.10696947255821608 0.0 0.0 0.0 0.0 35 0.10971227954688828 0.0 0.0 0.0 0.0 36 0.11108368304122439 0.0 0.0 0.0 0.0 37 0.1138264900298966 0.0 0.0 0.0 0.0 38 0.1138264900298966 0.0 0.0 0.0 0.0 39 0.1138264900298966 0.0 0.0 0.0 0.0 40 0.1151978935242327 0.0 0.0 0.0 0.0 41 0.1151978935242327 0.0 0.0 0.0 0.0 42 0.12479771798458543 0.0 0.0 0.0 0.0 43 0.12616912147892154 0.0 0.0 0.0 0.0 44 0.13165473545626594 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATTGA 30 9.802279E-7 50.389584 40 GCGCATT 95 0.0 50.389584 38 CGCTTCT 25 1.9750638E-5 50.389584 40 AGCCTTC 20 4.0151554E-4 50.354618 39 CGCATTG 85 0.0 50.354614 39 GTCTAAT 15 0.0081678 50.354614 39 CTCATAC 15 0.0081678 50.354614 39 CGCTTGC 15 0.0081678 50.354614 39 AGCGCTT 15 0.008212745 50.284824 37 GAGCCGA 15 0.008212745 50.284824 37 TGCTCGT 35 4.969843E-8 50.284824 37 ACCATAG 20 4.056626E-4 50.25 34 CATAGCC 20 4.056626E-4 50.25 36 CTCACTA 20 4.070525E-4 50.215225 45 TATATTC 25 2.0243291E-5 50.180496 14 CACGATT 25 2.0243291E-5 50.180496 47 ATATTCC 25 2.0243291E-5 50.180496 15 TAATCCG 15 0.008280506 50.180496 42 CGACTCC 15 0.008303186 50.14582 44 ACCTGGT 205 0.0 50.076603 11 >>END_MODULE