FastQCFastQC Report
Fri 17 Jun 2016
SRR1525909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4389
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGGTCTACCTGCTGGGCTGAGTTCTCTCTCTTG140.3189792663476874No Hit
TTAACTGGGAGATTTTGTGTTTTTCTCACAGAAATGTTAT70.1594896331738437No Hit
TTAACTGGGCTCGTCTTAATCAGCAACATCTTTTGATACA70.1594896331738437No Hit
TTAACTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT60.1367053998632946No Hit
TTAACTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA60.1367053998632946No Hit
TTAACTGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC50.11392116655274549No Hit
TTAACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT50.11392116655274549No Hit
TTAACTGGGGATCCAGTGACAGATACCATTAGATTCAAGA50.11392116655274549No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC50.11392116655274549No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC50.11392116655274549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGGT200.002382730634.3139535
CTGGGGA200.002382730634.3139535
CTGGGCA453.270543E-934.3139535
CTGGGAT200.002382730634.3139535
TGGGGGG453.270543E-934.3139536
GGAAGCA357.3925185E-734.3139538
ACTGGGT357.3925185E-734.3139534
ACTGGGG1150.034.3139534
GGGCCTG200.002382730634.3139537
CTGGGGG600.034.313955
CTGGGCC301.0987602E-534.313955
CTGGGAC251.622678E-434.313955
CTGGGAA950.034.313955
GAAGCAG251.622678E-434.313959
TGGGCAG301.0987602E-534.313956
ACTGGGC950.034.313954
TGGGAAG502.1464075E-1034.313956
AGCAGTG200.00252517133.9195411
AAGCAGT200.00252517133.9195410
AACTGGG4150.033.900533
Produced by FastQC (version 0.11.5)