##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525909_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4389
Sequences flagged as poor quality	0
Sequence length	40
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.922989291410342	33.0	30.0	34.0	23.0	37.0
2	25.245386192754612	28.0	22.0	33.0	4.0	36.0
3	19.97721576668945	21.0	13.0	27.0	4.0	33.0
4	22.917976760082023	26.0	16.0	31.0	4.0	35.0
5	20.228981544771017	17.0	15.0	30.0	4.0	35.0
6	22.43358395989975	25.0	17.0	30.0	4.0	34.0
7	24.25381635907952	29.0	20.0	33.0	4.0	36.0
8	22.472772841193894	26.0	14.0	31.0	4.0	35.0
9	23.505582137161085	27.0	17.0	32.0	4.0	36.0
10	18.07837776258829	18.0	8.0	27.0	4.0	33.0
11	23.249031670084303	28.0	10.0	34.0	4.0	37.0
12	23.268626110731375	28.0	10.0	34.0	4.0	37.0
13	23.581225791752107	29.0	10.0	34.0	4.0	37.0
14	23.827295511506037	29.0	4.0	34.0	4.0	37.0
15	21.771246297562087	26.0	4.0	33.0	4.0	36.0
16	19.048302574618365	21.0	4.0	31.0	4.0	35.0
17	19.894281157439053	23.0	4.0	32.0	4.0	35.0
18	19.202323991797677	22.0	4.0	31.0	4.0	35.0
19	16.77010708589656	15.0	4.0	29.0	4.0	33.0
20	18.145363408521302	19.0	4.0	31.0	4.0	35.0
21	15.177717019822284	12.0	4.0	27.0	4.0	33.0
22	15.344953292321714	10.0	4.0	28.0	4.0	33.0
23	15.528822055137844	10.0	4.0	28.0	4.0	33.0
24	14.9045340624288	4.0	4.0	28.0	4.0	33.0
25	14.802688539530644	4.0	4.0	28.0	4.0	33.0
26	12.41535657325131	4.0	4.0	22.0	4.0	32.0
27	12.380724538619276	4.0	4.0	22.0	4.0	32.0
28	12.338801549327865	4.0	4.0	23.0	4.0	32.0
29	11.667578035999089	4.0	4.0	21.0	4.0	31.0
30	10.945089997721576	4.0	4.0	18.0	4.0	30.0
31	11.358851674641148	4.0	4.0	21.0	4.0	32.0
32	10.902027796764639	4.0	4.0	18.0	4.0	31.0
33	10.740943267259057	4.0	4.0	17.0	4.0	31.0
34	10.432444748234222	4.0	4.0	16.0	4.0	31.0
35	10.302346776030987	4.0	4.0	15.0	4.0	31.0
36	10.661654135338345	4.0	4.0	13.0	4.0	34.0
37	10.68922305764411	4.0	4.0	13.0	4.0	34.0
38	10.480519480519481	4.0	4.0	4.0	4.0	33.0
39	10.059694691273638	4.0	4.0	4.0	4.0	32.0
40	10.119389382547277	4.0	4.0	4.0	4.0	32.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-17.63949326535532
1	2	-18.495425391977122
1	3	-14.447195588574907
1	4	-15.724882429192775
1	5	-14.814291232394684
1	6	-16.20593821886925
1	7	-17.213159924366817
1	8	-16.063565808393392
1	9	-16.49548688859034
1	10	-11.121619153515706
1	11	-17.256151256151256
1	12	-16.139757337171133
1	13	-17.382251592596425
1	14	-16.53629135870515
1	15	-13.375500106534593
1	16	-11.66424947976672
1	17	-12.001623280933622
1	18	-12.33302699854424
1	19	-9.009585975965289
1	20	-10.01924091234436
1	21	-5.706949978501709
1	22	-7.749586715966022
1	23	-7.811817939404143
1	24	-8.489894620067034
1	25	-7.826308525446461
1	26	-5.292383446693791
1	27	-5.458119785705992
1	28	-5.394778752537372
1	29	-5.083972285696421
1	30	-5.907115011425357
1	31	-3.6736681262543307
1	32	-2.8982167576995153
1	33	-2.555319871699183
1	34	-2.361118096462924
1	35	-2.2543971354316206
1	36	-2.6211493806321418
1	37	-3.309850685712755
1	38	-1.9823702797840745
1	39	-2.1974692931589495
1	40	-1.9877833851971793
4	1	4.582728956866902
4	2	9.504574608022878
4	3	-10.669417810797128
4	4	9.275117570807225
4	5	-5.036513454616905
4	6	10.349617336686304
4	7	-18.990937702144596
4	8	-17.174676919504503
4	9	-17.82882022192367
4	10	-12.232730264626817
4	11	-17.7005957005957
4	12	-17.695312892726687
4	13	-17.715584925929758
4	14	-17.86962469203848
4	15	-15.819944550979038
4	16	-12.997582813100053
4	17	-13.66828994760029
4	18	-12.777471442988684
4	19	-10.1206970870764
4	20	-11.13035202345547
4	21	-8.484727756279486
4	22	-8.971808938188245
4	23	-8.922929050515254
4	24	-8.712116842289257
4	25	-8.71519741433535
4	26	-6.959050113360458
4	27	-7.2358975634837694
4	28	-7.394778752537372
4	29	-6.861750063474199
4	30	-6.573781678092024
4	31	-7.118112570698775
4	32	-6.34266120214396
4	33	-5.999764316143628
4	34	-5.805562540907369
4	35	-5.698841579876065
4	36	-6.065593825076586
4	37	-5.86540624126831
4	38	-5.426814724228519
4	39	-5.641913737603394
4	40	-5.432227829641624
6	1	-1.217271043133099
6	2	1.2045746080228774
6	3	8.330582189202872
6	4	-5.924882429192774
6	5	-2.836513454616906
6	6	3.4496173366863054
6	7	1.409062297855403
6	8	-1.0746769195045012
6	9	1.1711797780763291
6	10	5.267269735373183
6	11	5.299404299404301
6	12	2.8046871072733133
6	13	3.884415074070244
6	14	5.830375307961518
6	15	5.580055449020961
6	16	-0.39758281310005117
6	17	4.131710052399711
6	18	6.922528557011315
6	19	2.2793029129235993
6	20	5.269647976544528
6	21	9.215272243720513
6	22	8.528191061811755
6	23	14.877070949484747
6	24	9.287883157710743
6	25	13.184802585664649
6	26	16.54094988663954
6	27	8.46410243651623
6	28	13.805221247462628
6	29	11.638249936525801
6	30	11.026218321907978
6	31	15.581887429301224
6	32	16.257338797856043
6	33	12.700235683856372
6	34	10.494437459092632
6	35	10.201158420123933
6	36	9.734406174923414
6	37	8.13459375873169
6	38	13.373185275771482
6	39	13.158086262396607
6	40	13.167772170358377
7	1	3.182728956866903
7	2	7.804574608022875
7	3	8.830582189202872
7	4	7.775117570807225
7	5	3.3634865453830933
7	6	9.549617336686303
7	7	3.909062297855403
7	8	4.725323080495496
7	9	7.171179778076329
7	10	9.067269735373184
7	11	6.399404299404303
7	12	10.304687107273313
7	13	7.984415074070242
7	14	8.630375307961518
7	15	9.08005544902096
7	16	12.702417186899947
7	17	12.631710052399711
7	18	7.722528557011316
7	19	6.1793029129236015
7	20	11.86964797654453
7	21	10.615272243720515
7	22	15.828191061811756
7	23	13.977070949484744
7	24	13.387883157710744
7	25	14.58480258566465
7	26	11.94094988663954
7	27	13.364102436516232
7	28	17.00522124746263
7	29	11.8382499365258
7	30	10.826218321907975
7	31	7.081887429301224
7	32	4.457338797856041
7	33	4.800235683856373
7	34	6.8944374590926305
7	35	-2.0988415798760656
7	36	-1.665593825076586
7	37	-0.5654062412683096
7	38	-0.12681472422851847
7	39	-2.441913737603394
7	40	-1.7322278296416238
9	1	-0.032655658517715125
9	2	3.4276515310998015
9	3	2.022889881510565
9	4	2.890502186191842
9	5	-2.9595903776938286
9	6	1.811155798224764
9	7	7.0090622978554045
9	8	8.286861542033957
9	9	8.17117977807633
9	10	5.921115889219337
9	11	7.453250453250455
9	12	7.15084095342716
9	13	6.592107381762549
9	14	0.053452231038441766
9	15	9.949286218251732
9	16	11.1562633407461
9	17	11.024017744707404
9	18	8.530220864703622
9	19	7.110072143692831
9	20	9.40810951500607
9	21	10.976810705258973
9	22	7.105114138734832
9	23	11.2309171033309
9	24	11.90326777309536
9	25	6.2078795087415735
9	26	4.194796040485695
9	27	2.7641024365162306
9	28	1.9129135551549368
9	29	2.369019167295031
9	30	0.8877567834464379
9	31	0.8818874293012247
9	32	4.272723413240655
9	33	1.1540818377025257
9	34	0.6559759206310929
9	35	3.3780814970470114
9	36	2.857483098000337
9	37	4.211516835654766
9	38	4.188569891156096
9	39	2.8196247239350676
9	40	0.798541401127606
10	1	0.5827289568669016
10	2	1.5045746080228781
10	3	-9.669417810797128
10	4	2.2751175708072253
10	5	-4.036513454616905
10	6	9.349617336686304
10	7	7.0090622978554045
10	8	11.825323080495497
10	9	7.171179778076329
10	10	-1.2327302646268166
10	11	1.2994042994043014
10	12	3.3046871072733133
10	13	11.284415074070242
10	14	6.130375307961518
10	15	8.180055449020962
10	16	13.002417186899947
10	17	18.33171005239971
10	18	18.222528557011316
10	19	15.8793029129236
10	20	17.869647976544528
10	21	12.515272243720514
10	22	20.028191061811754
10	23	23.077070949484746
10	24	24.287883157710745
10	25	14.28480258566465
10	26	23.04094988663954
10	27	16.764102436516232
10	28	17.60522124746263
10	29	19.138249936525803
10	30	18.426218321907974
10	31	25.881887429301223
10	32	19.657338797856042
10	33	19.000235683856374
10	34	21.194437459092633
10	35	21.301158420123933
10	36	21.934406174923414
10	37	14.13459375873169
10	38	7.573185275771481
10	39	22.358086262396604
10	40	7.567772170358376
12	1	-7.417271043133098
12	2	-1.4954253919771219
12	3	-7.669417810797128
12	4	0.2751175708072253
12	5	-4.036513454616905
12	6	0.349617336686304
12	7	3.0090622978554045
12	8	7.825323080495497
12	9	2.171179778076329
12	10	1.7672697353731834
12	11	9.299404299404301
12	12	4.304687107273313
12	13	7.2844150740702425
12	14	11.130375307961518
12	15	13.180055449020962
12	16	18.002417186899947
12	17	17.33171005239971
12	18	18.222528557011316
12	19	10.8793029129236
12	20	17.869647976544528
12	21	20.515272243720514
12	22	-0.9718089381882447
12	23	6.077070949484746
12	24	20.287883157710745
12	25	18.28480258566465
12	26	6.040949886639542
12	27	14.76410243651623
12	28	17.60522124746263
12	29	21.138249936525803
12	30	21.426218321907974
12	31	21.881887429301223
12	32	-6.34266120214396
12	33	-5.999764316143628
12	34	-5.805562540907369
12	35	-5.698841579876065
12	36	-6.065593825076586
12	37	-5.86540624126831
12	38	-5.426814724228519
12	39	-5.641913737603394
12	40	-5.432227829641624
13	1	-5.144543770405825
13	2	-13.586334482886214
13	3	-9.123963265342583
13	4	-12.27033697464732
13	5	-1.672877090980542
13	6	-9.832200845131878
13	7	-9.809119520326414
13	8	-8.08376782859541
13	9	-10.283365676469126
13	10	-7.141821173717725
13	11	-3.7915047915047886
13	12	-3.604403801817597
13	13	-6.2610394713843025
13	14	-3.5059883284021183
13	15	-3.6381263691608545
13	16	1.3660535505363107
13	17	0.6044373251269839
13	18	0.677074011556769
13	19	-0.02978799616730754
13	20	2.3241934310899826
13	21	4.15163588008415
13	22	2.5736456072663003
13	23	0.3497982222120193
13	24	0.015155884983469647
13	25	-2.9879246870626233
13	26	-2.5045046588150033
13	27	-5.054079381665588
13	28	-1.4856878434464633
13	29	-2.316295518019654
13	30	-2.7555998599102054
13	31	-1.1181125706987753
13	32	-2.0699339294166865
13	33	-0.3634006797799909
13	34	-2.3510170863619146
13	35	-2.5170233980578836
13	36	2.5707698112870503
13	37	0.952775576913508
13	38	2.6640943666805725
13	39	2.4489953533056976
13	40	2.6586812612674677
15	1	4.832728956866902
15	2	5.254574608022878
15	3	7.580582189202872
15	4	2.5251175708072253
15	5	12.963486545383095
15	6	11.599617336686304
15	7	13.009062297855404
15	8	14.575323080495497
15	9	13.67117977807633
15	10	9.267269735373183
15	11	6.799404299404301
15	12	2.8046871072733133
15	13	5.0344150740702425
15	14	6.880375307961518
15	15	-1.069944550979038
15	16	10.502417186899947
15	17	2.5817100523997105
15	18	4.222528557011316
15	19	12.1293029129236
15	20	10.36964797654453
15	21	12.765272243720514
15	22	11.528191061811755
15	23	12.327070949484746
15	24	13.287883157710743
15	25	6.78480258566465
15	26	12.790949886639542
15	27	11.51410243651623
15	28	5.105221247462628
15	29	6.638249936525801
15	30	-3.3237816780920237
15	31	2.1318874293012247
15	32	2.65733879785604
15	33	2.2502356838563724
15	34	0.19443745909263122
15	35	-2.448841579876065
15	36	6.434406174923414
15	37	2.1345937587316897
15	38	-1.4268147242285192
15	39	7.858086262396606
15	40	6.817772170358376
16	1	-0.31727104313309695
16	2	3.804574608022879
16	3	3.1305821892028725
16	4	4.875117570807227
16	5	-3.7365134546169045
16	6	5.349617336686304
16	7	6.309062297855405
16	8	9.325323080495497
16	9	6.071179778076328
16	10	3.867269735373185
16	11	8.899404299404303
16	12	6.504687107273313
16	13	9.584415074070243
16	14	10.930375307961516
16	15	9.280055449020963
16	16	14.302417186899948
16	17	14.231710052399709
16	18	11.822528557011317
16	19	9.8793029129236
16	20	11.16964797654453
16	21	7.0152722437205135
16	22	5.228191061811755
16	23	9.977070949484744
16	24	8.887883157710744
16	25	9.48480258566465
16	26	5.540949886639542
16	27	4.46410243651623
16	28	-0.49477875253737125
16	29	-0.2617500634741994
16	30	2.3262183219079766
16	31	-3.1181125706987753
16	32	0.4573387978560408
16	33	1.3002356838563731
16	34	-0.8055625409073688
16	35	1.6011584201239355
16	36	2.7344061749234143
16	37	5.03459375873169
16	38	0.6731852757714805
16	39	2.4580862623966055
16	40	2.6677721703583757
17	1	3.0827289568669016
17	2	5.83790794135621
17	3	2.0805821892028717
17	4	6.691784237473893
17	5	-0.4531801212835731
17	6	5.516284003352972
17	7	6.0090622978554045
17	8	3.9919897471621653
17	9	5.337846444742997
17	10	3.1006030687065156
17	11	7.549404299404301
17	12	3.8880204406066454
17	13	6.95108174073691
17	14	10.797041974628183
17	15	5.513388782354294
17	16	4.669083853566615
17	17	8.915043385733043
17	18	9.222528557011316
17	19	11.6293029129236
17	20	10.702981309877861
17	21	6.431938910387181
17	22	10.028191061811755
17	23	7.243737616151414
17	24	9.45454982437741
17	25	5.868135918997982
17	26	1.7909498866395417
17	27	6.347435769849563
17	28	4.5218879141292945
17	29	0.05491660319246705
17	30	-0.7404483447586898
17	31	4.131887429301225
17	32	0.9073387978560401
17	33	5.083569017189706
17	34	4.944437459092631
17	35	5.051158420123935
17	36	6.6010728415900815
17	37	7.8012604253983575
17	38	6.573185275771481
17	39	8.274752929063274
17	40	8.734438837025044
18	1	6.9827289568669
18	2	10.10457460802288
18	3	9.430582189202873
18	4	14.075117570807222
18	5	16.063486545383096
18	6	11.549617336686307
18	7	12.809062297855402
18	8	11.325323080495497
18	9	13.77117977807633
18	10	14.167269735373182
18	11	3.4994042994043006
18	12	5.604687107273314
18	13	5.684415074070241
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102	39	4.2152291195394636
102	40	4.2820578846440895
103	1	2.8327289568669016
103	2	7.504574608022878
103	3	0.33058218920287175
103	4	4.525117570807225
103	5	-1.5365134546169052
103	6	0.599617336686304
103	7	0.25906229785540447
103	8	-8.424676919504503
103	9	-0.3288202219236709
103	10	-6.482730264626817
103	11	3.0494042994043014
103	12	0.8046871072733133
103	13	4.2844150740702425
103	14	1.6303753079615184
103	15	6.930055449020962
103	16	7.002417186899947
103	17	8.58171005239971
103	18	9.472528557011316
103	19	3.8793029129236007
103	20	-5.880352023455471
103	21	-6.9847277562794865
103	22	-3.4718089381882447
103	23	-7.422929050515254
103	24	-3.962116842289257
103	25	-4.46519741433535
103	26	-3.7090501133604583
103	27	-4.4858975634837694
103	28	-3.1447787525373716
103	29	-2.861750063474199
103	30	-2.5737816780920237
103	31	-0.8681125706987753
103	32	-0.0926612021439599
103	33	0.2502356838563724
103	34	0.4444374590926312
103	35	1.0511584201239348
103	36	1.6844061749234136
103	37	2.1345937587316897
103	38	2.323185275771481
103	39	2.108086262396606
103	40	2.317772170358376
104	1	2.0827289568669016
104	2	5.504574608022878
104	3	1.8305821892028717
104	4	0.5251175708072253
104	5	-3.5365134546169052
104	6	-0.15038266331369599
104	7	2.7590622978554045
104	8	2.8253230804954974
104	9	-5.328820221923671
104	10	6.767269735373183
104	11	1.5494042994043014
104	12	2.8046871072733133
104	13	2.7844150740702425
104	14	4.630375307961518
104	15	2.180055449020962
104	16	6.752417186899947
104	17	4.8317100523997105
104	18	6.722528557011316
104	19	7.129302912923601
104	20	-1.8803520234554707
104	21	-0.7347277562794865
104	22	0.028191061811755347
104	23	0.07707094948474591
104	24	0.03788315771074302
104	25	4.03480258566465
104	26	1.7909498866395417
104	27	3.2641024365162306
104	28	6.605221247462628
104	29	1.638249936525801
104	30	0.6762183219079763
104	31	-4.868112570698775
104	32	-4.34266120214396
104	33	-3.2497643161436276
104	34	-3.805562540907369
104	35	-2.448841579876065
104	36	0.9344061749234136
104	37	-0.36540624126831034
104	38	1.5731852757714808
104	39	1.108086262396606
104	40	1.317772170358376
105	1	0.8327289568669016
105	2	-3.745425391977122
105	3	-4.419417810797128
105	4	0.2751175708072253
105	5	-7.536513454616905
105	6	-1.400382663313696
105	7	-1.4909377021445955
105	8	2.3253230804954974
105	9	0.17117977807632911
105	10	-4.982730264626817
105	11	0.049404299404301355
105	12	-0.9453128927266867
105	13	3.7844150740702425
105	14	4.380375307961518
105	15	-4.319944550979038
105	16	0.7524171868999474
105	17	-2.6682899476002895
105	18	-5.527471442988684
105	19	4.379302912923601
105	20	3.8696479765445293
105	21	4.5152722437205135
105	22	0.27819106181175535
105	23	2.827070949484746
105	24	0.787883157710743
105	25	5.03480258566465
105	26	-5.959050113360458
105	27	-4.7358975634837694
105	28	-6.394778752537372
105	29	-5.111750063474199
105	30	-3.5737816780920237
105	31	-5.118112570698775
105	32	-2.09266120214396
105	33	1.0002356838563724
105	34	-3.555562540907369
105	35	1.3011584201239348
105	36	1.6844061749234136
105	37	1.8845937587316897
105	38	2.323185275771481
105	39	2.108086262396606
105	40	2.317772170358376
106	1	4.582728956866902
106	2	8.171241274689542
106	3	4.663915522536204
106	4	5.6084509041405575
106	5	12.963486545383095
106	6	4.016284003352972
106	7	1.0090622978554045
106	8	3.4919897471621653
106	9	1.5045131114096613
106	10	2.4339364020398513
106	11	7.6327376327376335
106	12	-1.0286462260600189
106	13	0.9510817407369103
106	14	6.463708641294851
106	15	1.84672211568763
106	16	-2.9975828131000526
106	17	-3.6682899476002895
106	18	4.222528557011316
106	19	0.8793029129236007
106	20	6.869647976544529
106	21	0.18193891038717958
106	22	1.3615243951450893
106	23	-0.5895957171819202
106	24	-0.045450175622590905
106	25	-0.048530747668683816
106	26	3.3742832199728756
106	27	3.4307691031828966
106	28	2.9385545807959623
106	29	3.138249936525801
106	30	3.75955165524131
106	31	3.2152207626345586
106	32	2.990672131189374
106	33	3.3335690171897063
106	34	3.527770792425965
106	35	3.6344917534572687
106	36	3.9344061749234136
106	37	4.13459375873169
106	38	3.9065186091048147
106	39	4.358086262396606
106	40	3.90110550369171
107	1	3.3327289568669016
107	2	2.254574608022878
107	3	6.330582189202872
107	4	-2.7248824291927747
107	5	10.713486545383095
107	6	2.099617336686304
107	7	7.0090622978554045
107	8	6.825323080495497
107	9	7.171179778076329
107	10	6.517269735373183
107	11	9.049404299404301
107	12	6.804687107273313
107	13	8.534415074070242
107	14	8.130375307961518
107	15	8.930055449020962
107	16	7.502417186899947
107	17	11.33171005239971
107	18	9.222528557011316
107	19	10.3793029129236
107	20	3.6196479765445293
107	21	-3.7347277562794865
107	22	0.5281910618117553
107	23	-0.4229290505152541
107	24	0.03788315771074302
107	25	-1.21519741433535
107	26	-2.4590501133604583
107	27	2.2641024365162306
107	28	0.8552212474626284
107	29	1.638249936525801
107	30	-0.5737816780920237
107	31	1.8818874293012247
107	32	8.15733879785604
107	33	7.000235683856372
107	34	1.1944374590926312
107	35	1.3011584201239348
107	36	1.4344061749234136
107	37	1.8845937587316897
107	38	2.323185275771481
107	39	2.108086262396606
107	40	2.317772170358376
108	1	1.9160622902002338
108	2	3.8379079413562103
108	3	3.663915522536204
108	4	2.6084509041405575
108	5	0.2968198787164269
108	6	7.349617336686304
108	7	10.34239563118874
108	8	9.825323080495497
108	9	11.17117977807633
108	10	8.100603068706516
108	11	12.966070966070966
108	12	14.971353773939978
108	13	10.617748407403578
108	14	15.797041974628183
108	15	12.846722115687626
108	16	14.669083853566615
108	17	12.665043385733043
108	18	12.555861890344648
108	19	9.212636246256933
108	20	8.536314643211197
108	21	-2.4847277562794865
108	22	-1.6384756048549107
108	23	-5.922929050515254
108	24	-2.712116842289257
108	25	-1.0485307476686838
108	26	-1.6257167800271244
108	27	-3.5692308968171025
108	28	-0.7281120858707055
108	29	2.138249936525801
108	30	-2.5737816780920237
108	31	1.2152207626345586
108	32	-1.009327868810626
108	33	2.3568385637240397E-4
108	34	2.8611041257592973
108	35	-5.698841579876065
108	36	-6.065593825076586
108	37	-5.86540624126831
108	38	-5.426814724228519
108	39	-5.641913737603394
108	40	-5.432227829641624
112	1	-5.0172710431331
112	2	-12.495425391977122
112	3	-5.269417810797128
112	4	1.4751175708072246
112	5	-5.836513454616906
112	6	0.9496173366863054
112	7	0.8090622978554052
112	8	-1.5746769195045012
112	9	0.9711797780763298
112	10	0.36726973537318486
112	11	2.2994042994043014
112	12	4.904687107273315
112	13	2.684415074070241
112	14	2.930375307961519
112	15	1.9800554490209628
112	16	-5.397582813100053
112	17	-4.868289947600289
112	18	-6.777471442988684
112	19	-5.9206970870764
112	20	1.0696479765445286
112	21	-0.4847277562794865
112	22	2.2281910618117546
112	23	0.6770709494847456
112	24	0.287883157710743
112	25	0.6848025856646505
112	26	5.44094988663954
112	27	6.364102436516232
112	28	4.605221247462628
112	29	6.5382499365257996
112	30	7.826218321907975
112	31	10.081887429301224
112	32	8.85733879785604
112	33	9.600235683856374
112	34	5.594437459092632
112	35	9.501158420123934
112	36	12.534406174923415
112	37	4.93459375873169
112	38	13.173185275771482
112	39	7.158086262396607
112	40	5.567772170358376
114	1	-0.4172710431330984
114	2	1.5045746080228781
114	3	-2.6694178107971283
114	4	-2.7248824291927747
114	5	-6.036513454616905
114	6	7.349617336686304
114	7	3.0090622978554045
114	8	7.825323080495497
114	9	3.171179778076329
114	10	-3.2327302646268166
114	11	5.299404299404301
114	12	6.304687107273313
114	13	8.284415074070242
114	14	-9.869624692038482
114	15	7.180055449020962
114	16	9.002417186899947
114	17	12.33171005239971
114	18	-12.777471442988684
114	19	-10.1206970870764
114	20	-11.13035202345547
114	21	-8.484727756279486
114	22	-8.971808938188245
114	23	-8.922929050515254
114	24	-8.712116842289257
114	25	-8.71519741433535
114	26	-6.959050113360458
114	27	-7.2358975634837694
114	28	-7.394778752537372
114	29	-6.861750063474199
114	30	-6.573781678092024
114	31	-7.118112570698775
114	32	-6.34266120214396
114	33	-5.999764316143628
114	34	-5.805562540907369
114	35	-5.698841579876065
114	36	-6.065593825076586
114	37	-5.86540624126831
114	38	-5.426814724228519
114	39	-5.641913737603394
114	40	-5.432227829641624
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	639.0
5	64.0
6	87.0
7	76.0
8	44.0
9	64.0
10	101.0
11	134.0
12	181.0
13	163.0
14	180.0
15	168.0
16	178.0
17	214.0
18	203.0
19	172.0
20	175.0
21	172.0
22	160.0
23	183.0
24	185.0
25	185.0
26	173.0
27	145.0
28	114.0
29	92.0
30	69.0
31	29.0
32	18.0
33	8.0
34	9.0
35	1.0
36	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.13222196848595	0.3425439598081754	0.20552637588490524	0.3197076958209637
8	97.33424470266576	1.0025062656641603	0.7518796992481203	0.9113693324219639
9	95.73643410852713	2.165982672138623	1.2539899680802553	0.84359325125399
10	37.935748462064254	33.78901799954431	12.235133287764867	16.040100250626566
11	28.84483937115516	28.57142857142857	20.80200501253133	21.781727044884942
12	36.46308113035551	16.81859617137648	23.336371923427528	23.381950774840472
13	29.801777170198225	16.24515835042151	27.8879015721121	26.065162907268167
14	19.161540214171794	24.743677375256322	32.80929596719071	23.28548644338118
15	23.76395534290271	25.951241740715425	29.414445203918888	20.870357712462976
16	26.08794714057872	21.234905445431764	28.86762360446571	23.809523809523807
17	25.563909774436087	25.4272043745728	24.196855775803144	24.81203007518797
18	26.72590567327409	24.698108908635223	23.76395534290271	24.81203007518797
19	20.883901992214334	17.655140828944354	16.968170368674144	44.49278681016716
20	26.11073137388927	24.743677375256322	23.467760309865575	25.677830940988837
21	23.650034176349966	21.645021645021643	26.83982683982684	27.865117338801547
22	22.761449077238552	19.22989291410344	26.247436773752565	31.761221234905445
23	26.481312670920694	22.470373746581586	28.350045578851415	22.69826800364631
24	21.964000911369332	22.123490544543177	25.31328320802005	30.59922533606744
25	24.840328467153284	23.357664233576642	23.403284671532848	28.39872262773723
26	19.788797061524335	23.576675849403124	29.40771349862259	27.226813590449954
27	18.94232961021199	20.515158422612263	28.21974014132665	32.3227718258491
28	15.667046750285063	21.345496009122005	33.112884834663625	29.874572405929307
29	17.398262459990853	18.26703246456333	34.79652491998171	29.538180155464104
30	17.067032715625714	18.371082132235188	37.039579043697096	27.522306108442002
31	17.466849565614996	20.27892089620485	38.36305441243713	23.89117512574303
32	20.323536113009798	17.042606516290725	38.778765094554565	23.85509227614491
33	18.14038286235187	17.251595259799455	41.79580674567001	22.81221513217867
34	15.94896331738437	17.407154249259513	46.206425153793575	20.437457279562544
35	14.900888585099112	19.981772613351563	43.517885623148786	21.599453178400545
36	12.508544087491455	22.123490544543177	41.99134199134199	23.376623376623375
37	12.901755185776157	21.72327330749943	41.281057670389785	24.093913836334625
38	15.174299384825702	21.645021645021643	40.25974025974026	22.920938710412393
39	15.629984051036683	20.369104579630896	39.895192526771474	24.105718842560947
40	13.397129186602871	23.194349510138984	41.125541125541126	22.28298017771702
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	3.0
10	5.0
11	7.0
12	9.0
13	9.0
14	13.0
15	17.0
16	27.0
17	37.0
18	37.0
19	39.0
20	41.0
21	52.0
22	63.0
23	63.0
24	70.0
25	77.0
26	87.0
27	97.0
28	97.0
29	128.5
30	160.0
31	192.0
32	224.0
33	224.0
34	244.5
35	265.0
36	290.5
37	316.0
38	316.0
39	310.0
40	304.0
41	298.0
42	292.0
43	292.0
44	273.5
45	255.0
46	255.5
47	256.0
48	256.0
49	273.5
50	291.0
51	297.0
52	303.0
53	303.0
54	303.5
55	304.0
56	300.0
57	296.0
58	296.0
59	278.0
60	260.0
61	216.0
62	172.0
63	172.0
64	161.5
65	151.0
66	124.0
67	97.0
68	97.0
69	73.0
70	49.0
71	39.5
72	30.0
73	30.0
74	21.5
75	13.0
76	7.5
77	2.0
78	2.0
79	2.0
80	2.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.22784233310549099
8	0.0
9	0.0683526999316473
10	0.0
11	0.0
12	0.022784233310549098
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5012531328320802
20	0.0
21	0.0
22	0.0
23	0.022784233310549098
24	0.0
25	0.11392116655274549
26	0.7518796992481203
27	0.045568466621098196
28	0.09113693324219639
29	0.3417634996582365
30	0.4101161995898838
31	0.3417634996582365
32	0.0
33	0.022784233310549098
34	0.0
35	0.0
36	0.0
37	0.045568466621098196
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	4389.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.26087947140579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.22554412819899	92.61790840738209
2	1.7220760583592443	3.2809295967190706
3	0.5022721836881129	1.4354066985645932
4	0.31093039942597467	1.1847801321485532
5	0.1195886151638364	0.5696058327637276
6	0.04783544606553456	0.2734107997265892
7	0.04783544606553456	0.3189792663476874
8	0.0	0.0
9	0.0	0.0
>10	0.02391772303276728	0.3189792663476874
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTAACTGGGGTCTACCTGCTGGGCTGAGTTCTCTCTCTTG	14	0.3189792663476874	No Hit
TTAACTGGGAGATTTTGTGTTTTTCTCACAGAAATGTTAT	7	0.1594896331738437	No Hit
TTAACTGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	7	0.1594896331738437	No Hit
TTAACTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.1367053998632946	No Hit
TTAACTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA	6	0.1367053998632946	No Hit
TTAACTGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC	5	0.11392116655274549	No Hit
TTAACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	5	0.11392116655274549	No Hit
TTAACTGGGGATCCAGTGACAGATACCATTAGATTCAAGA	5	0.11392116655274549	No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	5	0.11392116655274549	No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC	5	0.11392116655274549	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGGGGT	20	0.0023827306	34.313953	5
CTGGGGA	20	0.0023827306	34.313953	5
CTGGGCA	45	3.270543E-9	34.313953	5
CTGGGAT	20	0.0023827306	34.313953	5
TGGGGGG	45	3.270543E-9	34.313953	6
GGAAGCA	35	7.3925185E-7	34.313953	8
ACTGGGT	35	7.3925185E-7	34.313953	4
ACTGGGG	115	0.0	34.313953	4
GGGCCTG	20	0.0023827306	34.313953	7
CTGGGGG	60	0.0	34.31395	5
CTGGGCC	30	1.0987602E-5	34.31395	5
CTGGGAC	25	1.622678E-4	34.31395	5
CTGGGAA	95	0.0	34.31395	5
GAAGCAG	25	1.622678E-4	34.31395	9
TGGGCAG	30	1.0987602E-5	34.31395	6
ACTGGGC	95	0.0	34.31395	4
TGGGAAG	50	2.1464075E-10	34.31395	6
AGCAGTG	20	0.002525171	33.91954	11
AAGCAGT	20	0.002525171	33.91954	10
AACTGGG	415	0.0	33.90053	3
>>END_MODULE