FastQCFastQC Report
Fri 17 Jun 2016
SRR1525907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3641
Sequences flagged as poor quality0
Sequence length40
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT581.5929689645701732No Hit
TTAACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG461.2633891787970337No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT320.8788794287283713No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC300.8239494644328481No Hit
TTAACTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.35704476792090084No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG110.3021148036253776No Hit
TTAACTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG110.3021148036253776No Hit
TTAACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC100.274649821477616No Hit
TTAACTGGGCTCACAGATCCTCTTCTGAGATGAGTTTTTG100.274649821477616No Hit
TTAACTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT90.24718483932985444No Hit
TTAACTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.21971985718209283No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA70.19225487503433122No Hit
TTAACTGGGGCTTTAACTGGGTCACAGATCCTCTTCTGAG70.19225487503433122No Hit
TTAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA50.137324910738808No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGAC50.137324910738808No Hit
TTAACTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT50.137324910738808No Hit
TTAACTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.137324910738808No Hit
TTAACTGGGATTGTGTTAACTTTCAGTTCTCTGTCTCTTT50.137324910738808No Hit
TTAACTGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA40.10985992859104642No Hit
TTAACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC40.10985992859104642No Hit
TTAACTGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG40.10985992859104642No Hit
TTAACTGGGTATTCCAAATTAACTAGGCCTATCTGGTTTC40.10985992859104642No Hit
TTAACTGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGC40.10985992859104642No Hit
TTAACTGGGACTTTGAAAAAATTAGAGTGTTCAAAGCAGG40.10985992859104642No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG40.10985992859104642No Hit
TTAACTGGGAACTCATCCCTTGACATCGTGCTTCACGATA40.10985992859104642No Hit
TTAACTGGGGGGGAGGTAGTGACGAAAAATAACAATACAG40.10985992859104642No Hit
TTAACTGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAA40.10985992859104642No Hit
TTAACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGG40.10985992859104642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGGG750.034.05
CTGGGAT404.9250957E-834.05
TGGGGGG251.6557542E-434.06
TGGGGGA357.4491254E-734.06
TGGGGAG200.002437427634.06
TGGGGAA251.6557542E-434.06
TGGGATT200.002437427634.06
ACTGGGC404.9250957E-834.04
ACTGGGA750.034.04
GGGGAGT200.002437427634.07
TGGCTCA200.002437427634.023
ACTGGGG1850.033.081084
TTAACTG3600.032.5833321
TAACTGG3550.032.5633772
AACTGGG3400.032.53
CTGGGGA700.031.5714285
CTGGGGC353.2065167E-529.1428575
ACTGGGT407.9778896E-525.54
Produced by FastQC (version 0.11.5)