##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525907_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3641
Sequences flagged as poor quality	0
Sequence length	40
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.828893161219444	30.0	4.0	33.0	4.0	34.0
2	15.849766547651743	14.0	4.0	28.0	4.0	33.0
3	14.235924196649272	10.0	4.0	24.0	4.0	31.0
4	13.29717110683878	9.0	4.0	24.0	4.0	31.0
5	13.010711343037627	5.0	4.0	22.0	4.0	30.0
6	12.90332326283988	4.0	4.0	22.0	4.0	30.0
7	15.247184839329854	4.0	4.0	29.0	4.0	33.0
8	14.980499862675089	4.0	4.0	28.0	4.0	33.0
9	14.606152156001098	4.0	4.0	27.0	4.0	33.0
10	12.898104916231805	4.0	4.0	23.0	4.0	32.0
11	13.28206536665751	4.0	4.0	26.0	4.0	33.0
12	12.762153254600385	4.0	4.0	25.0	4.0	32.0
13	11.616039549574293	4.0	4.0	20.0	4.0	31.0
14	12.180444932710794	4.0	4.0	23.0	4.0	33.0
15	11.78797033781928	4.0	4.0	22.0	4.0	33.0
16	9.733315023345234	4.0	4.0	13.0	4.0	29.0
17	9.027190332326285	4.0	4.0	10.0	4.0	26.0
18	8.819005767646251	4.0	4.0	9.0	4.0	26.0
19	8.608624004394397	4.0	4.0	8.0	4.0	25.0
20	9.032408678934358	4.0	4.0	4.0	4.0	27.0
21	7.971985718209283	4.0	4.0	4.0	4.0	24.0
22	7.860752540510848	4.0	4.0	4.0	4.0	23.0
23	8.014556440538314	4.0	4.0	4.0	4.0	24.0
24	7.78687173853337	4.0	4.0	4.0	4.0	23.0
25	8.085690744301017	4.0	4.0	4.0	4.0	25.0
26	8.263663828618512	4.0	4.0	4.0	4.0	26.0
27	8.237572095578138	4.0	4.0	4.0	4.0	26.0
28	8.422136775611095	4.0	4.0	4.0	4.0	27.0
29	7.684427355122219	4.0	4.0	4.0	4.0	22.0
30	7.9703378192804175	4.0	4.0	4.0	4.0	24.0
31	8.47926393847844	4.0	4.0	4.0	4.0	29.0
32	8.413897280966767	4.0	4.0	4.0	4.0	28.0
33	8.335622081845647	4.0	4.0	4.0	4.0	28.0
34	8.344685525954407	4.0	4.0	4.0	4.0	29.0
35	8.221367756110958	4.0	4.0	4.0	4.0	28.0
36	8.206536665751168	4.0	4.0	4.0	4.0	29.0
37	8.098324636088986	4.0	4.0	4.0	4.0	28.0
38	8.079923098049987	4.0	4.0	4.0	4.0	29.0
39	7.92859104641582	4.0	4.0	4.0	4.0	28.0
40	7.958802526778357	4.0	4.0	4.0	4.0	29.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	2.569551465937014
1	2	8.051503817467673
1	3	-2.0801565235300163
1	4	10.625209629727705
1	5	-4.9085977610074005
1	6	0.26427063565617637
1	7	-9.959306088824162
1	8	-9.336567515483178
1	9	-8.56599805816673
1	10	-7.359678273533694
1	11	-7.407327478411817
1	12	-6.996633052355943
1	13	-5.97063013960604
1	14	-6.439981170101651
1	15	-6.194253938832253
1	16	-4.420812922017738
1	17	-3.794917766303308
1	18	-3.665360710240229
1	19	-3.664819718434175
1	20	-4.0865781808552875
1	21	-3.3276983391441233
1	22	-3.0857253188578495
1	23	-3.271212689586185
1	24	-3.109914750577402
1	25	-3.1361022845962605
1	26	-3.244386336555012
1	27	-2.96460383659179
1	28	-3.451068743839829
1	29	-3.2227139326536927
1	30	-3.287172131750445
1	31	-3.8355289289024235
1	32	-3.9848316342292263
1	33	-3.939740279800521
1	34	-3.73888006237404
1	35	-3.9544548155993953
1	36	-3.54829795371964
1	37	-3.78033673154155
1	38	-3.575823808052724
1	39	-3.149099695485238
1	40	-3.619337790723333
2	1	11.569551465937014
2	2	-10.28182951586566
2	3	-9.41348985686335
2	4	-8.374790370272295
2	5	-8.575264427674067
2	6	-8.40239603101049
2	7	-9.959306088824162
2	8	-9.336567515483178
2	9	-8.56599805816673
2	10	-7.359678273533694
2	11	-7.407327478411817
2	12	-6.996633052355943
2	13	-5.97063013960604
2	14	-6.439981170101651
2	15	-6.194253938832253
2	16	-4.420812922017738
2	17	-3.794917766303308
2	18	-3.665360710240229
2	19	-3.664819718434175
2	20	-4.0865781808552875
2	21	-3.3276983391441233
2	22	-3.0857253188578495
2	23	-3.271212689586185
2	24	-3.109914750577402
2	25	-3.1361022845962605
2	26	-3.244386336555012
2	27	-2.96460383659179
2	28	-3.451068743839829
2	29	-3.2227139326536927
2	30	-3.287172131750445
2	31	-3.8355289289024235
2	32	-3.9848316342292263
2	33	-3.939740279800521
2	34	-3.73888006237404
2	35	-3.9544548155993953
2	36	-3.54829795371964
2	37	-3.78033673154155
2	38	-3.575823808052724
2	39	-3.149099695485238
2	40	-3.619337790723333
3	1	8.569551465937014
3	2	-0.28182951586565963
3	3	14.58651014313665
3	4	13.625209629727705
3	5	7.4247355723259325
3	6	5.09760396898951
3	7	3.540693911175838
3	8	7.663432484516822
3	9	9.93400194183327
3	10	2.140321726466306
3	11	17.092672521588185
3	12	15.003366947644057
3	13	7.02936986039396
3	14	3.0600188298983486
3	15	1.8057460611677474
3	16	1.5791870779822617
3	17	4.705082233696692
3	18	-0.665360710240229
3	19	7.835180281565825
3	20	4.4134218191447125
3	21	1.1723016608558767
3	22	3.4142746811421505
3	23	7.728787310413815
3	24	0.39008524942259815
3	25	4.3638977154037395
3	26	6.255613663444988
3	27	9.53539616340821
3	28	1.548931256160171
3	29	6.277286067346307
3	30	4.712827868249555
3	31	9.164471071097577
3	32	11.015168365770773
3	33	6.560259720199479
3	34	12.26111993762596
3	35	9.045545184400606
3	36	4.45170204628036
3	37	3.71966326845845
3	38	1.924176191947276
3	39	8.350900304514763
3	40	9.880662209276668
4	1	-13.097115200729652
4	2	-10.28182951586566
4	3	-9.41348985686335
4	4	-8.374790370272295
4	5	-8.575264427674067
4	6	-8.40239603101049
4	7	-9.959306088824162
4	8	-9.336567515483178
4	9	-8.56599805816673
4	10	-7.359678273533694
4	11	-7.407327478411817
4	12	-6.996633052355943
4	13	-5.97063013960604
4	14	-6.439981170101651
4	15	-6.194253938832253
4	16	-4.420812922017738
4	17	-3.794917766303308
4	18	-3.665360710240229
4	19	-3.664819718434175
4	20	-4.0865781808552875
4	21	-3.3276983391441233
4	22	-3.0857253188578495
4	23	-3.271212689586185
4	24	-3.109914750577402
4	25	-3.1361022845962605
4	26	-3.244386336555012
4	27	-2.96460383659179
4	28	-3.451068743839829
4	29	-3.2227139326536927
4	30	-3.287172131750445
4	31	-3.8355289289024235
4	32	-3.9848316342292263
4	33	-3.939740279800521
4	34	-3.73888006237404
4	35	-3.9544548155993953
4	36	-3.54829795371964
4	37	-3.78033673154155
4	38	-3.575823808052724
4	39	-3.149099695485238
4	40	-3.619337790723333
5	1	8.236218132603682
5	2	-4.28182951586566
5	3	-0.41348985686335027
5	4	-5.7081237036056285
5	5	8.0914022389926
5	6	8.264270635656178
5	7	-2.959306088824162
5	8	-0.0032341821498445
5	9	5.4340019418332695
5	10	5.640321726466306
5	11	13.25933918825485
5	12	12.003366947644057
5	13	11.362703193727292
5	14	11.89335216323168
5	15	7.1390793945010795
5	16	-2.0874795886844053
5	17	-2.4615844329699748
5	18	2.334639289759771
5	19	-2.331486385100842
5	20	2.9134218191447125
5	21	-2.6610316724774563
5	22	2.9142746811421505
5	23	3.728787310413815
5	24	-2.109914750577402
5	25	-1.1361022845962605
5	26	1.7556136634449881
5	27	3.03539616340821
5	28	4.882264589493505
5	29	2.7772860673463073
5	30	1.7128278682495548
5	31	6.4978044044309105
5	32	4.68183503243744
5	33	4.060259720199479
5	34	-1.405546729040707
5	35	6.378878517733939
5	36	4.118368712947026
5	37	6.552996601791784
5	38	6.090842858613942
5	39	-3.149099695485238
5	40	-3.619337790723333
6	1	4.369551465937015
6	2	8.71817048413434
6	3	3.5865101431366497
6	4	4.6252096297277046
6	5	2.024735572325932
6	6	0.5976039689895103
6	7	2.2406939111758373
6	8	10.86343248451682
6	9	4.834001941833268
6	10	1.8403217264663052
6	11	-5.407327478411817
6	12	1.803366947644058
6	13	0.6293698603939593
6	14	2.3600188298983493
6	15	2.0057460611677467
6	16	0.5791870779822617
6	17	2.6050822336966926
6	18	1.334639289759771
6	19	1.9351802815658248
6	20	5.513421819144712
6	21	1.6723016608558767
6	22	5.9142746811421505
6	23	4.9287873104138145
6	24	2.2900852494225985
6	25	6.8638977154037395
6	26	5.1556136634449885
6	27	2.4353961634082104
6	28	5.74893125616017
6	29	2.577286067346308
6	30	4.912827868249554
6	31	6.564471071097577
6	32	5.015168365770774
6	33	6.660259720199479
6	34	6.061119937625961
6	35	4.045545184400605
6	36	3.0517020462803597
6	37	5.419663268458449
6	38	0.024176191947275605
6	39	1.4509003045147617
6	40	-0.019337790723333192
7	1	-0.5971152007296538
7	2	4.0515038174676725
7	3	-0.7468231901966842
7	4	3.9585429630610367
7	5	-1.7419310943407336
7	6	-5.569062697677157
7	7	-6.959306088824162
7	8	-2.6699008488165124
7	9	-0.23266472483339662
7	10	0.14032172646630592
7	11	3.759339188254849
7	12	1.8367002809773911
7	13	6.52936986039396
7	14	2.2266854965650147
7	15	2.1390793945010813
7	16	4.912520411315596
7	17	5.038415567030026
7	18	6.834639289759771
7	19	4.501846948232491
7	20	1.5800884858113786
7	21	3.5056349941892107
7	22	0.5809413478088175
7	23	1.062120643747149
7	24	-0.6099147505774019
7	25	3.5305643820704056
7	26	3.088946996778322
7	27	1.7020628300748761
7	28	4.048931256160171
7	29	2.7772860673463073
7	30	4.212827868249555
7	31	5.331137737764243
7	32	3.0151683657707737
7	33	3.560259720199479
7	34	2.594453270959294
7	35	4.545545184400605
7	36	5.95170204628036
7	37	3.052996601791784
7	38	2.590842858613942
7	39	6.350900304514762
7	40	2.0473288759433332
8	1	-6.001877105491557
8	2	-8.710400944437088
8	3	-8.699204142577635
8	4	-6.231933227415152
8	5	-7.5752644276740675
8	6	-6.545253173867633
8	7	-8.673591803109876
8	8	-8.050853229768894
8	9	-6.994569486738159
8	10	-6.502535416390837
8	11	-6.693041764126103
8	12	-6.568061623784514
8	13	-4.256344425320326
8	14	-3.725695455815937
8	15	-4.622825367403681
8	16	-3.1350986363034528
8	17	-1.7949177663033078
8	18	-1.3796464245259434
8	19	-2.664819718434175
8	20	-1.2294353237124307
8	21	-1.3276983391441233
8	22	0.19998896685643608
8	23	-1.9854984038718992
8	24	-1.5384861791488307
8	25	-2.1361022845962605
8	26	-1.6729577651264407
8	27	-1.96460383659179
8	28	-2.5939258866969723
8	29	-1.7941425040822638
8	30	-0.5728864174647308
8	31	-1.2641003574738523
8	32	-0.5562602056577974
8	33	0.060259720199479005
8	34	-0.16745149094546896
8	35	0.3312594701148912
8	36	0.8802734748517889
8	37	0.3625204113155922
8	38	0.5670333348044183
8	39	0.9937574473719044
8	40	0.23780506641952392
9	1	11.013995910381457
9	2	11.60705937302323
9	3	8.919843476469982
9	4	-2.7081237036056294
9	5	11.98029112788149
9	6	10.4864928578784
9	7	13.151805022286949
9	8	2.4412102622946
9	9	11.989557497388827
9	10	6.4180995042440845
9	11	6.7037836326992934
9	12	4.447811392088502
9	13	3.918258749282849
9	14	5.337796607676127
9	15	3.8057460611677474
9	16	1.2458537446489277
9	17	-1.5726955440810855
9	18	-0.332027376906896
9	19	-2.4425974962119525
9	20	-1.1976892919663982
9	21	-1.3276983391441233
9	22	0.025385792253261208
9	23	1.6176761993027045
9	24	2.001196360533709
9	25	1.7527866042926288
9	26	3.088946996778322
9	27	-0.07571494770290066
9	28	0.9933757006046156
9	29	1.7772860673463073
9	30	1.7128278682495548
9	31	0.05335995998646581
9	32	-0.09594274534033698
9	33	0.28248194242170044
9	34	-0.5166578401518178
9	35	-0.732232593377173
9	36	-0.10385350927519532
9	37	-1.447003398208217
9	38	-0.1313793636082794
9	39	-0.26021080659634865
9	40	-0.3971155685011105
10	1	6.694551465937014
10	2	10.21817048413434
10	3	8.21151014313665
10	4	7.3752096297277046
10	5	9.799735572325933
10	6	3.0976039689895103
10	7	13.290693911175838
10	8	13.288432484516822
10	9	15.80900194183327
10	10	12.515321726466306
10	11	14.342672521588183
10	12	12.003366947644057
10	13	13.65436986039396
10	14	4.935018829898349
10	15	8.180746061167747
10	16	8.954187077982262
10	17	9.330082233696693
10	18	8.584639289759771
10	19	8.460180281565826
10	20	11.413421819144713
10	21	10.547301660855876
10	22	6.2892746811421505
10	23	8.603787310413814
10	24	8.640085249422597
10	25	10.73889771540374
10	26	10.255613663444988
10	27	10.91039616340821
10	28	13.29893125616017
10	29	9.652286067346306
10	30	7.712827868249555
10	31	9.289471071097577
10	32	9.765168365770773
10	33	7.185259720199479
10	34	3.13611993762596
10	35	10.045545184400606
10	36	8.95170204628036
10	37	10.21966326845845
10	38	9.299176191947275
10	39	9.225900304514763
10	40	9.255662209276668
12	1	8.569551465937014
12	2	15.71817048413434
12	3	14.58651014313665
12	4	7.6252096297277046
12	5	-1.5752644276740675
12	6	4.09760396898951
12	7	19.040693911175836
12	8	18.16343248451682
12	9	19.43400194183327
12	10	17.640321726466304
12	11	14.592672521588183
12	12	20.503366947644057
12	13	18.52936986039396
12	14	23.060018829898347
12	15	-6.194253938832253
12	16	-4.420812922017738
12	17	-3.794917766303308
12	18	-3.665360710240229
12	19	-3.664819718434175
12	20	-4.0865781808552875
12	21	-3.3276983391441233
12	22	-3.0857253188578495
12	23	-3.271212689586185
12	24	-3.109914750577402
12	25	-3.1361022845962605
12	26	-3.244386336555012
12	27	-2.96460383659179
12	28	-3.451068743839829
12	29	-3.2227139326536927
12	30	-3.287172131750445
12	31	-3.8355289289024235
12	32	-3.9848316342292263
12	33	-3.939740279800521
12	34	-3.73888006237404
12	35	-3.9544548155993953
12	36	-3.54829795371964
12	37	-3.78033673154155
12	38	-3.575823808052724
12	39	-3.149099695485238
12	40	-3.619337790723333
13	1	7.569551465937014
13	2	4.71817048413434
13	3	7.58651014313665
13	4	0.6252096297277046
13	5	-6.5752644276740675
13	6	-4.40239603101049
13	7	10.040693911175838
13	8	8.663432484516822
13	9	9.43400194183327
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110	36	-3.54829795371964
110	37	-3.78033673154155
110	38	-3.575823808052724
110	39	-3.149099695485238
110	40	-3.619337790723333
111	1	5.969551465937013
111	2	11.91817048413434
111	3	12.186510143136651
111	4	11.825209629727704
111	5	11.624735572325932
111	6	5.79760396898951
111	7	13.240693911175837
111	8	11.463432484516822
111	9	15.23400194183327
111	10	15.840321726466305
111	11	10.392672521588183
111	12	8.603366947644059
111	13	10.82936986039396
111	14	5.160018829898348
111	15	5.805746061167747
111	16	-4.420812922017738
111	17	-3.794917766303308
111	18	-3.665360710240229
111	19	-3.664819718434175
111	20	-4.0865781808552875
111	21	-3.3276983391441233
111	22	-3.0857253188578495
111	23	-3.271212689586185
111	24	-3.109914750577402
111	25	-3.1361022845962605
111	26	-3.244386336555012
111	27	-2.96460383659179
111	28	-3.451068743839829
111	29	-3.2227139326536927
111	30	-3.287172131750445
111	31	-3.8355289289024235
111	32	-3.9848316342292263
111	33	-3.939740279800521
111	34	-3.73888006237404
111	35	-3.9544548155993953
111	36	-3.54829795371964
111	37	-3.78033673154155
111	38	-3.575823808052724
111	39	-3.149099695485238
111	40	-3.619337790723333
112	1	-17.430448534062986
112	2	-10.28182951586566
112	3	-9.41348985686335
112	4	-8.374790370272295
112	5	-8.575264427674067
112	6	-8.40239603101049
112	7	-9.959306088824162
112	8	-9.336567515483178
112	9	-8.56599805816673
112	10	-7.359678273533694
112	11	-7.407327478411817
112	12	-6.996633052355943
112	13	-5.97063013960604
112	14	-6.439981170101651
112	15	-6.194253938832253
112	16	-4.420812922017738
112	17	-3.794917766303308
112	18	-3.665360710240229
112	19	-3.664819718434175
112	20	-4.0865781808552875
112	21	-3.3276983391441233
112	22	-3.0857253188578495
112	23	-3.271212689586185
112	24	-3.109914750577402
112	25	-3.1361022845962605
112	26	-3.244386336555012
112	27	-2.96460383659179
112	28	-3.451068743839829
112	29	-3.2227139326536927
112	30	-3.287172131750445
112	31	-3.8355289289024235
112	32	-3.9848316342292263
112	33	-3.939740279800521
112	34	-3.73888006237404
112	35	-3.9544548155993953
112	36	-3.54829795371964
112	37	-3.78033673154155
112	38	-3.575823808052724
112	39	-3.149099695485238
112	40	-3.619337790723333
117	1	10.069551465937014
117	2	-10.28182951586566
117	3	-9.41348985686335
117	4	-8.374790370272295
117	5	-8.575264427674067
117	6	-8.40239603101049
117	7	-9.959306088824162
117	8	-9.336567515483178
117	9	-8.56599805816673
117	10	-7.359678273533694
117	11	-7.407327478411817
117	12	-6.996633052355943
117	13	-5.97063013960604
117	14	-6.439981170101651
117	15	-6.194253938832253
117	16	-4.420812922017738
117	17	-3.794917766303308
117	18	-3.665360710240229
117	19	-3.664819718434175
117	20	-4.0865781808552875
117	21	-3.3276983391441233
117	22	-3.0857253188578495
117	23	-3.271212689586185
117	24	-3.109914750577402
117	25	-3.1361022845962605
117	26	-3.244386336555012
117	27	-2.96460383659179
117	28	-3.451068743839829
117	29	-3.2227139326536927
117	30	-3.287172131750445
117	31	-3.8355289289024235
117	32	-3.9848316342292263
117	33	-3.939740279800521
117	34	-3.73888006237404
117	35	-3.9544548155993953
117	36	-3.54829795371964
117	37	-3.78033673154155
117	38	-3.575823808052724
117	39	-3.149099695485238
117	40	-3.619337790723333
119	1	3.319551465937014
119	2	0.46817048413434037
119	3	-1.6634898568633503
119	4	-4.6247903702722954
119	5	-1.8252644276740675
119	6	5.09760396898951
119	7	-6.959306088824162
119	8	0.6634324845168216
119	9	1.1840019418332695
119	10	3.640321726466306
119	11	-4.907327478411817
119	12	0.0033669476440572055
119	13	0.5293698603939596
119	14	-6.439981170101651
119	15	-6.194253938832253
119	16	-4.420812922017738
119	17	-3.794917766303308
119	18	-3.665360710240229
119	19	-3.664819718434175
119	20	-4.0865781808552875
119	21	-3.3276983391441233
119	22	-3.0857253188578495
119	23	-3.271212689586185
119	24	-3.109914750577402
119	25	-3.1361022845962605
119	26	-3.244386336555012
119	27	-2.96460383659179
119	28	-3.451068743839829
119	29	-3.2227139326536927
119	30	-3.287172131750445
119	31	-3.8355289289024235
119	32	-3.9848316342292263
119	33	-3.939740279800521
119	34	-3.73888006237404
119	35	-3.9544548155993953
119	36	-3.54829795371964
119	37	-3.78033673154155
119	38	-3.575823808052724
119	39	-3.149099695485238
119	40	-3.619337790723333
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1633.0
5	93.0
6	118.0
7	64.0
8	95.0
9	136.0
10	170.0
11	163.0
12	120.0
13	95.0
14	71.0
15	59.0
16	37.0
17	35.0
18	38.0
19	36.0
20	59.0
21	101.0
22	94.0
23	100.0
24	84.0
25	70.0
26	68.0
27	35.0
28	22.0
29	24.0
30	9.0
31	7.0
32	3.0
33	0.0
34	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	98.73522133626615	0.577398955182843	0.43992301347264234	0.2474566950783613
8	98.57025020621391	0.6048941435248831	0.6323893318669233	0.192466318394281
9	97.25199230557845	1.2091233855454795	1.044242923880187	0.49464138499587795
10	46.6355396868992	28.618511397967588	13.787421038176326	10.95852787695688
11	31.428571428571427	24.807692307692307	24.203296703296704	19.560439560439562
12	30.714285714285715	23.434065934065934	24.697802197802197	21.153846153846153
13	22.509631260319207	21.876719867914144	27.490368739680793	28.123280132085853
14	21.214619400934325	31.052486946963455	29.541082715031603	18.191810937070624
15	31.255149684152705	25.130458665201864	27.767096951386982	15.847294699258446
16	30.074155451798955	32.02416918429003	25.048063718758584	12.85361164515243
17	25.35017852238396	26.558637736885473	32.29881900576765	15.792364734962922
18	24.642857142857146	26.923076923076923	28.653846153846153	19.78021978021978
19	24.044016506189823	29.160935350756535	25.061898211829437	21.73314993122421
20	24.718483932985443	28.481186487228783	31.035429826970613	15.764899752815161
21	22.10931062894809	26.613567701180997	30.925569898379567	20.35155177149135
22	21.340291128810765	23.48255973633617	33.91925295248558	21.25789618236748
23	21.593406593406595	25.879120879120883	33.104395604395606	19.423076923076923
24	22.905795111233175	28.20653666575117	26.888217522658607	21.999450700357045
25	23.197578425976886	26.55476059438635	29.939460649422124	20.30820033021464
26	26.352097130242825	26.82119205298013	25.137969094922735	21.688741721854303
27	20.6782464846981	30.46594982078853	25.19988971601875	23.655913978494624
28	23.404840484048407	32.67326732673268	23.1023102310231	20.81958195819582
29	29.001103752759384	30.242825607064017	22.737306843267106	18.018763796909493
30	25.659340659340657	27.362637362637365	28.48901098901099	18.48901098901099
31	26.430143014301432	30.033003300330037	24.394939493949394	19.141914191419144
32	30.17360154312483	27.693579498484432	24.49710664094792	17.635712317442824
33	28.46153846153846	28.324175824175825	24.505494505494504	18.708791208791208
34	19.307882449876406	28.426256522933258	33.86432298819006	18.401538039000275
35	17.659983521010712	30.678385059049713	35.429826970612474	16.231804449327107
36	21.95054945054945	32.06043956043956	28.15934065934066	17.82967032967033
37	23.406593406593405	29.67032967032967	29.697802197802197	17.225274725274726
38	24.807692307692307	31.291208791208792	26.126373626373628	17.774725274725274
39	23.81213952210931	29.442460862400438	26.00933809393024	20.73606152156001
40	24.114254325734688	27.27272727272727	29.634715737434767	18.978302664103268
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	0.5
5	0.0
6	1.5
7	3.0
8	3.0
9	14.5
10	26.0
11	25.5
12	25.0
13	25.0
14	25.5
15	26.0
16	24.5
17	23.0
18	23.0
19	26.5
20	30.0
21	39.0
22	48.0
23	48.0
24	49.0
25	50.0
26	62.5
27	75.0
28	75.0
29	92.0
30	109.0
31	124.0
32	139.0
33	139.0
34	153.5
35	168.0
36	219.5
37	271.0
38	271.0
39	280.5
40	290.0
41	288.5
42	287.0
43	287.0
44	309.0
45	331.0
46	358.0
47	385.0
48	385.0
49	366.0
50	347.0
51	321.0
52	295.0
53	295.0
54	291.0
55	287.0
56	248.5
57	210.0
58	210.0
59	154.0
60	98.0
61	87.0
62	76.0
63	76.0
64	52.5
65	29.0
66	18.0
67	7.0
68	7.0
69	5.0
70	3.0
71	2.0
72	1.0
73	1.0
74	1.0
75	1.0
76	0.5
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.10985992859104642
8	0.10985992859104642
9	0.05492996429552321
10	0.0
11	0.027464982147761604
12	0.027464982147761604
13	0.19225487503433122
14	0.05492996429552321
15	0.0
16	0.0
17	0.0
18	0.027464982147761604
19	0.16478989288656962
20	0.0
21	0.0
22	0.0
23	0.027464982147761604
24	0.0
25	0.19225487503433122
26	0.46690469651194727
27	0.38450975006866245
28	0.137324910738808
29	0.46690469651194727
30	0.027464982147761604
31	0.137324910738808
32	0.32957978577313923
33	0.027464982147761604
34	0.0
35	0.0
36	0.027464982147761604
37	0.027464982147761604
38	0.027464982147761604
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	3641.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.69568799780279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.8011274663326	83.13650096127438
2	3.6955840901973067	6.481735786871738
3	0.5950516755402443	1.5655039824224115
4	0.3445036016285625	1.2084592145015105
5	0.15659254619480112	0.6866245536940401
6	0.0	0.0
7	0.06263701847792046	0.38450975006866245
8	0.03131850923896023	0.21971985718209283
9	0.03131850923896023	0.24718483932985444
>10	0.2505480739116818	4.476792090085142
>50	0.03131850923896023	1.5929689645701732
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTAACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	58	1.5929689645701732	No Hit
TTAACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	46	1.2633891787970337	No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	32	0.8788794287283713	No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	30	0.8239494644328481	No Hit
TTAACTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13	0.35704476792090084	No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	11	0.3021148036253776	No Hit
TTAACTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG	11	0.3021148036253776	No Hit
TTAACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC	10	0.274649821477616	No Hit
TTAACTGGGCTCACAGATCCTCTTCTGAGATGAGTTTTTG	10	0.274649821477616	No Hit
TTAACTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT	9	0.24718483932985444	No Hit
TTAACTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8	0.21971985718209283	No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA	7	0.19225487503433122	No Hit
TTAACTGGGGCTTTAACTGGGTCACAGATCCTCTTCTGAG	7	0.19225487503433122	No Hit
TTAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA	5	0.137324910738808	No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGAC	5	0.137324910738808	No Hit
TTAACTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT	5	0.137324910738808	No Hit
TTAACTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.137324910738808	No Hit
TTAACTGGGATTGTGTTAACTTTCAGTTCTCTGTCTCTTT	5	0.137324910738808	No Hit
TTAACTGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA	4	0.10985992859104642	No Hit
TTAACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC	4	0.10985992859104642	No Hit
TTAACTGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG	4	0.10985992859104642	No Hit
TTAACTGGGTATTCCAAATTAACTAGGCCTATCTGGTTTC	4	0.10985992859104642	No Hit
TTAACTGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGC	4	0.10985992859104642	No Hit
TTAACTGGGACTTTGAAAAAATTAGAGTGTTCAAAGCAGG	4	0.10985992859104642	No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG	4	0.10985992859104642	No Hit
TTAACTGGGAACTCATCCCTTGACATCGTGCTTCACGATA	4	0.10985992859104642	No Hit
TTAACTGGGGGGGAGGTAGTGACGAAAAATAACAATACAG	4	0.10985992859104642	No Hit
TTAACTGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAA	4	0.10985992859104642	No Hit
TTAACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGG	4	0.10985992859104642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.027464982147761604	0.0	0.0	0.0	0.0
15	0.027464982147761604	0.0	0.0	0.0	0.0
16	0.027464982147761604	0.0	0.0	0.0	0.0
17	0.027464982147761604	0.0	0.0	0.0	0.0
18	0.027464982147761604	0.0	0.0	0.0	0.0
19	0.05492996429552321	0.0	0.0	0.0	0.0
20	0.05492996429552321	0.0	0.0	0.0	0.0
21	0.05492996429552321	0.0	0.0	0.0	0.0
22	0.05492996429552321	0.0	0.0	0.0	0.0
23	0.05492996429552321	0.0	0.0	0.0	0.0
24	0.05492996429552321	0.0	0.0	0.0	0.0
25	0.05492996429552321	0.0	0.0	0.0	0.0
26	0.05492996429552321	0.0	0.0	0.0	0.0
27	0.05492996429552321	0.0	0.0	0.0	0.0
28	0.08239494644328481	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGGGGG	75	0.0	34.0	5
CTGGGAT	40	4.9250957E-8	34.0	5
TGGGGGG	25	1.6557542E-4	34.0	6
TGGGGGA	35	7.4491254E-7	34.0	6
TGGGGAG	20	0.0024374276	34.0	6
TGGGGAA	25	1.6557542E-4	34.0	6
TGGGATT	20	0.0024374276	34.0	6
ACTGGGC	40	4.9250957E-8	34.0	4
ACTGGGA	75	0.0	34.0	4
GGGGAGT	20	0.0024374276	34.0	7
TGGCTCA	20	0.0024374276	34.0	23
ACTGGGG	185	0.0	33.08108	4
TTAACTG	360	0.0	32.583332	1
TAACTGG	355	0.0	32.563377	2
AACTGGG	340	0.0	32.5	3
CTGGGGA	70	0.0	31.571428	5
CTGGGGC	35	3.2065167E-5	29.142857	5
ACTGGGT	40	7.9778896E-5	25.5	4
>>END_MODULE