FastQCFastQC Report
Fri 17 Jun 2016
SRR1525906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92657
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG25262.726183666641484No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT15761.7008968561468643No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC12961.3987070593695026No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG6810.7349687557335118No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC4690.5061679096020808No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT3860.4165902198430772No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG3420.3691032517780632No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCATTAGTTGGTCTTCCA3090.3334880257293027No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG2980.3216162837130492No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2480.2676538200028061No Hit
TAGAGAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT2120.22880084613143098No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA1960.21153285774415317No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG1930.2082951099215386No Hit
TAGAGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1670.18023462879221214No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1420.15325339693709056No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1370.14785715056606624No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC1320.14246090419504193No Hit
TAGAGAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1310.14138165492083707No Hit
TAGAGAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1280.13814390709822247No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC1210.13058916217878844No Hit
TAGAGAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA1110.11979666943673979No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCCTTAGTTGGTCTTCCA1080.1165589216141252No Hit
TAGAGAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC1070.11547967233992035No Hit
TAGAGAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT1070.11547967233992035No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT1040.11224192451730576No Hit
TAGAGAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC1020.11008342596889605No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC970.10468717959787172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATC204.00145E-450.40415240
CGCATTG850.050.40415239
AATTGGA204.00145E-450.40415239
TAATTGG204.00145E-450.40415238
GCTCAAT204.00145E-450.40415239
CGTTGTA150.00814312250.4041539
GTCTAAT150.00814312250.4041539
TCAATTC150.00814312250.4041541
TAATTAT150.00814312250.4041540
TCGTCTC1150.050.4041540
ACGTCTC150.00814312250.4041541
GCTCGTC1200.050.4041538
TCACGTC150.00814312250.4041539
GGCGCAT900.050.3766437
TTGTCCA1050.050.37663737
CGCCGCT150.00816072950.37663337
ATGCTCG1250.050.26688836
AGCGTCA150.0082314450.26688435
ATGCCCG150.0082314450.26688436
ATGAGTC204.066636E-450.23952546
Produced by FastQC (version 0.11.5)