##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525906_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92657
Sequences flagged as poor quality	0
Sequence length	56
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.61715790496131	38.0	36.0	39.0	33.0	40.0
2	34.18881466052214	38.0	35.0	39.0	17.0	40.0
3	35.33221451158574	38.0	35.0	39.0	28.0	40.0
4	33.3797122721435	38.0	33.0	39.0	13.0	40.0
5	34.60031082379097	38.0	33.0	39.0	25.0	40.0
6	32.610973806620116	38.0	32.0	39.0	11.0	40.0
7	35.48531681362444	38.0	35.0	39.0	28.0	40.0
8	35.45656561295962	38.0	35.0	39.0	28.0	40.0
9	35.58481280421339	38.0	35.0	39.0	29.0	40.0
10	34.00663738303636	38.0	32.0	39.0	22.0	40.0
11	33.772073345780676	38.0	32.0	39.0	23.0	40.0
12	33.16582665098158	38.0	31.0	39.0	19.0	40.0
13	32.714689661871205	37.0	30.0	39.0	18.0	40.0
14	33.34485252058668	38.0	32.0	39.0	20.0	40.0
15	32.10144943177526	36.0	29.0	39.0	16.0	40.0
16	32.63754492375104	37.0	30.0	39.0	19.0	40.0
17	31.68253882599264	36.0	28.0	38.0	16.0	40.0
18	31.27806857549888	35.0	27.0	38.0	15.0	40.0
19	32.283335311957	36.0	29.0	38.0	19.0	40.0
20	32.87965291343342	36.0	30.0	39.0	21.0	40.0
21	33.414366966338214	38.0	32.0	39.0	20.0	40.0
22	32.77037892442017	38.0	30.0	39.0	18.0	40.0
23	33.46322458098147	38.0	32.0	39.0	21.0	40.0
24	32.741746441175515	37.0	30.0	39.0	18.0	40.0
25	33.19778322199078	38.0	31.0	39.0	20.0	40.0
26	33.53931165481291	38.0	33.0	39.0	21.0	40.0
27	33.78838080231391	38.0	33.0	39.0	23.0	40.0
28	33.700216929104116	38.0	33.0	39.0	23.0	40.0
29	33.284770713491696	38.0	32.0	39.0	19.0	40.0
30	32.98487971766839	37.0	32.0	39.0	18.0	40.0
31	33.61252792557497	38.0	33.0	39.0	22.0	40.0
32	33.600159728892585	38.0	33.0	39.0	22.0	40.0
33	33.33015314547201	38.0	33.0	39.0	21.0	40.0
34	33.172043126801	37.0	33.0	39.0	19.0	40.0
35	33.29885491652007	38.0	33.0	39.0	20.0	40.0
36	32.40927290976397	36.0	31.0	38.0	16.0	40.0
37	32.20893186699332	36.0	31.0	38.0	14.0	40.0
38	31.392328696158952	36.0	30.0	38.0	6.0	40.0
39	30.225455173381395	35.0	27.0	38.0	4.0	40.0
40	28.175237704652645	34.0	20.0	38.0	4.0	39.0
41	25.92730176888956	31.0	15.0	37.0	4.0	39.0
42	26.835306560756337	31.0	19.0	37.0	4.0	39.0
43	27.260282547459987	32.0	21.0	38.0	4.0	39.0
44	26.250655643934078	31.0	17.0	37.0	4.0	39.0
45	27.154699590964526	32.0	21.0	38.0	4.0	39.0
46	25.08987987955578	30.0	15.0	36.0	4.0	39.0
47	24.31972759748319	29.0	10.0	36.0	4.0	39.0
48	24.806112867889098	29.0	14.0	36.0	4.0	39.0
49	23.852714851549262	28.0	8.0	35.0	4.0	39.0
50	24.52327401059823	29.0	11.0	36.0	4.0	39.0
51	24.715779703638148	30.0	4.0	36.0	4.0	39.0
52	24.649578553158424	30.0	4.0	36.0	4.0	39.0
53	24.4963683261923	30.0	4.0	36.0	4.0	39.0
54	24.012422159146098	30.0	4.0	36.0	4.0	39.0
55	23.921139255533852	30.0	4.0	36.0	4.0	39.0
56	22.67380769936432	28.0	4.0	35.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.0579921939156947
1	2	6.206495227074967
1	3	4.854245819175112
1	4	6.5187382729645975
1	5	5.407902466717545
1	6	7.989937119230749
1	7	4.53725189996414
1	8	4.507217037749882
1	9	4.460426617487613
1	10	5.918510489567311
1	11	-0.1277259314751653
1	12	-6.401142635529769
1	13	-17.504915604909804
1	14	-6.4122727960771435
1	15	1.8970704968071637
1	16	-1.8043907676150823
1	17	-3.9568609419606418
1	18	-4.616513825858242
1	19	-0.46117037643743686
1	20	-1.1320150328575842
1	21	5.606096458815593
1	22	7.045927779338815
1	23	5.861228257594934
1	24	6.12181896528698
1	25	5.696850933836302
1	26	5.869063044299178
1	27	5.110710844322494
1	28	5.36694056367082
1	29	5.641411659502523
1	30	6.034823735732537
1	31	4.644475265753243
1	32	4.218842063716238
1	33	4.447964968279024
1	34	3.65591978613811
1	35	3.9425108172867596
1	36	1.949624725854843
1	37	2.067475533301373
1	38	3.222216323270729
1	39	3.3180315142459236
1	40	-11.825154409181803
1	41	-1.8045716817757054
1	42	-3.035770025847846
1	43	-11.243529293921354
1	44	-15.693665309203546
1	45	-3.665777592409885
1	46	-5.6194417897916935
1	47	-11.07833322796343
1	48	-5.810233829769924
1	49	-14.166739052674668
1	50	-4.434803292421176
1	51	1.1514501034939322
1	52	1.5789302236696763
1	53	1.3935854308279971
1	54	1.628285929431101
1	55	1.7407412083291298
1	56	5.131921956584787
2	1	3.5271457089802567
2	2	6.623819473846851
2	3	4.995924441843691
2	4	6.346751185446664
2	5	5.456682954522421
2	6	8.192591351655427
2	7	4.712467107998577
2	8	4.791291643201816
2	9	4.623446703570828
2	10	6.380490403484096
2	11	4.070982820318232
2	12	-0.37639371156993917
2	13	1.3336783692652325
2	14	5.251823330178237
2	15	6.132185274425527
2	16	4.176957869400699
2	17	6.733060148426734
2	18	3.7114990866238244
2	19	5.697186868899724
2	20	4.660847234000379
2	21	6.295120849059494
2	22	7.170031079195336
2	23	6.255776320722628
2	24	6.29362671277622
2	25	5.6243975622437645
2	26	5.224514981171485
2	27	5.156980571725651
2	28	4.874652184904679
2	29	5.376347097092193
2	30	6.266172372748322
2	31	4.142143845380218
2	32	4.196962580215519
2	33	4.521494380043727
2	34	4.109650058734953
2	35	4.201836498778867
2	36	5.168060880804628
2	37	4.698573094276984
2	38	5.566011158277902
2	39	5.868426062309052
2	40	2.964121057102272
2	41	8.44112415753563
2	42	6.995973159231063
2	43	6.209124938503326
2	44	-5.044633745358496
2	45	6.155958419067879
2	46	5.384683030868278
2	47	1.5470254520939584
2	48	4.910175065495498
2	49	-2.2533602865340647
2	50	5.003683077736643
2	51	7.421536186707705
2	52	7.974374986940838
2	53	7.9861248856343074
2	54	7.53377373430915
2	55	7.5669607205242535
2	56	8.0257526595977
3	1	3.4075397052279115
3	2	5.892581199925651
3	3	4.750927568798041
3	4	6.101128921531718
3	5	4.907902466717545
3	6	7.6879009201357285
3	7	4.244831085484506
3	8	4.383536796422582
3	9	4.168005803007979
3	10	5.566700534816178
3	11	4.325516904120608
3	12	5.204438058286222
3	13	5.246592690716138
3	14	5.471022829865543
3	15	-0.07106675809480123
3	16	5.7887464872868755
3	17	6.682090792579327
3	18	7.683825540657594
3	19	5.861039276654569
3	20	5.475262493537585
3	21	6.452250304969439
3	22	6.794796557619353
3	23	5.993581198771405
3	24	6.291125149299045
3	25	5.7862174496734085
3	26	4.768761385173992
3	27	4.307543423508012
3	28	4.21705368584276
3	29	5.200612262820776
3	30	5.00616611883963
3	31	4.352431525180094
3	32	3.8745735870948224
3	33	3.7522636108129603
3	34	3.4526769505423367
3	35	4.020942189835779
3	36	5.203395464919698
3	37	5.218116558942402
3	38	5.36682416640798
3	39	7.373272841546072
3	40	7.957085409822724
3	41	11.415639479611926
3	42	9.913890607636318
3	43	9.678793481342602
3	44	10.28729245851893
3	45	9.790104760531293
3	46	11.12358234293832
3	47	11.934298747903838
3	48	11.591538417590556
3	49	11.454113134353992
3	50	11.442082077111255
3	51	10.211404854625155
3	52	10.068183617334832
3	53	10.107763409711858
3	54	10.887901314046488
3	55	9.705484798072721
3	56	9.93131863833441
4	1	3.477883834782574
4	2	6.284281604783949
4	3	4.852310834654993
4	4	6.702174805472339
4	5	5.30921825619123
4	6	7.909054766289575
4	7	4.686632704917706
4	8	4.4596164185548375
4	9	4.313754790862227
4	10	5.959919158297957
4	11	5.84441029143505
4	12	6.192123618340197
4	13	6.459480679919917
4	14	6.089275191538956
4	15	6.957442013835024
4	16	6.970764031146526
4	17	8.404594166398493
4	18	9.027449022438972
4	19	7.252064917680212
4	20	6.858697041445822
4	21	6.5600438272366475
4	22	6.973946355190204
4	23	6.493581198771405
4	24	7.000301937423199
4	25	6.3702255468394
4	26	5.532762734701652
4	27	4.688110225127449
4	28	4.882033442927781
4	29	5.382123733805933
4	30	5.106031166073095
4	31	3.4362709313879165
4	32	3.7467058408060225
4	33	3.6267575379384667
4	34	3.874573036912107
4	35	4.117433417905957
4	36	4.860615437929145
4	37	4.797738691196113
4	38	5.693578552372898
4	39	6.635755972450259
4	40	0.002631968527175843
4	41	10.155180640205721
4	42	9.353161862697043
4	43	9.465062037347998
4	44	9.324910542189645
4	45	8.625631076320765
4	46	11.686672761291895
4	47	11.712301446959174
4	48	11.064379173326053
4	49	11.148276427201491
4	50	11.566744695194924
4	51	10.315149793896406
4	52	10.145106694257912
4	53	9.818121034543164
4	54	10.529601718904786
4	55	9.911793839908078
4	56	9.551426600547636
5	1	3.3739185644639136
5	2	6.38211547517421
5	3	4.974894078478833
5	4	6.814106420223503
5	5	5.407902466717545
5	6	7.755143201663724
5	7	4.580519206886912
5	8	4.671082583968371
5	9	4.605734740736914
5	10	6.008746584005081
5	11	5.985119206580055
5	12	6.7472167082077235
5	13	6.4969851553943165
5	14	6.314167780153351
5	15	7.457844806531053
5	16	6.997930160756265
5	17	8.318799322145
5	18	8.527493777182976
5	19	7.342200972101981
5	20	6.869035387310483
5	21	6.373103261536684
5	22	6.437784522035887
5	23	6.097322695370043
5	24	6.878121486295381
5	25	5.80819547165143
5	26	5.7043971779526395
5	27	5.34770564224166
5	28	5.245441964231045
5	29	4.796373644296445
5	30	5.898369153899807
5	31	3.3519082389425208
5	32	3.446132980082787
5	33	4.598024992288622
5	34	3.917224307946835
5	35	3.925704094597684
5	36	5.370192953145764
5	37	4.780910907451034
5	38	5.2171643024624075
5	39	6.461839037255128
5	40	7.826656315107911
5	41	10.542667213782515
5	42	9.252195160226584
5	43	9.394125768103457
5	44	10.04658078923583
5	45	9.0230979578102
5	46	10.843493384277934
5	47	11.358291421896517
5	48	10.888895822090817
5	49	11.51638419662505
5	50	11.029732521904553
5	51	10.104131175922905
5	52	10.121297170448386
5	53	10.081860740952045
5	54	10.021993412424298
5	55	10.080026922614845
5	56	9.569597026612797
6	1	3.586575458207328
6	2	6.782613702634208
6	3	5.077940931022674
6	4	6.687441669816295
6	5	5.647339086435856
6	6	8.400251949388167
6	7	4.635947011811702
6	8	4.777722423002572
6	9	4.749262574405591
6	10	5.863829462226796
6	11	6.135737200256397
6	12	6.770978325530713
6	13	7.058257551676771
6	14	6.287292241205375
6	15	7.641374556459187
6	16	6.632643200901899
6	17	8.001610474775067
6	18	8.555710697754023
6	19	7.276712805004156
6	20	6.633622912461384
6	21	6.292716177125449
6	22	7.539921151335495
6	23	6.349215001588309
6	24	7.176914242834616
6	25	6.248839334830507
6	26	6.134597427066371
6	27	5.877695102814624
6	28	5.7119074236542495
6	29	5.873598900596143
6	30	6.30988587326361
6	31	4.3601960611136406
6	32	3.9131254108579157
6	33	4.327832408212743
6	34	4.526259471307956
6	35	5.143007917535314
6	36	5.514247758166359
6	37	5.903743138935173
6	38	6.791706795515964
6	39	7.937646261553301
6	40	4.821386601588703
6	41	11.78842748972388
6	42	10.630033619553977
6	43	9.902701029193807
6	44	10.910622180440196
6	45	9.617569549263685
6	46	11.595864755361575
6	47	12.472308859857613
6	48	11.32781505175452
6	49	11.95971082305027
6	50	11.877437801199374
6	51	10.955716880627318
6	52	11.180317961863544
6	53	11.25853986330521
6	54	11.267370436473357
6	55	10.520670785793921
6	56	10.4482019897248
7	1	3.8039072265954346
7	2	6.9246324819769285
7	3	5.551141244011717
7	4	7.303051998454791
7	5	5.630124688939766
7	6	8.42988809962291
7	7	4.636925102578523
7	8	4.86751115539694
7	9	4.692044264546439
7	10	6.409115064730706
7	11	6.371457075060778
7	12	6.811275665123823
7	13	7.198515767639211
7	14	7.109377530720241
7	15	7.817822130794088
7	16	7.309579820620211
7	17	8.358907031895566
7	18	8.744188788520844
7	19	7.988176028791326
7	20	6.717249673024767
7	21	6.939429792148928
7	22	7.820437583260382
7	23	7.139414532104741
7	24	7.397962756136653
7	25	6.8161319795879365
7	26	6.1533767697893715
7	27	5.637671628636216
7	28	5.100600694389772
7	29	6.219843032051543
7	30	6.23212765730117
7	31	5.229034089282656
7	32	4.802992390513623
7	33	5.15343882448817
7	34	4.8928478907132735
7	35	4.652886634280229
7	36	6.312369823894063
7	37	6.859142199968037
7	38	7.644601944185759
7	39	8.628080533853765
7	40	8.531871734609048
7	41	12.285297599270045
7	42	11.471582915328625
7	43	11.557532797581914
7	44	12.635583056809526
7	45	11.130382538309071
7	46	13.524757556613537
7	47	13.821585072690162
7	48	13.49858969964184
7	49	13.443963561704418
7	50	13.225735923265102
7	51	13.35937494009524
7	52	13.249273360924576
7	53	12.273895888344335
7	54	13.294952596097765
7	55	12.42824120832913
7	56	11.672771629787402
8	1	3.4284926328972603
8	2	6.358712083966978
8	3	4.775229137875726
8	4	6.623325630295589
8	5	5.191484556269785
8	6	8.078830885692557
8	7	4.1989068604558
8	8	4.361914140471569
8	9	4.5623800854419585
8	10	5.937825678329382
8	11	5.764284604082338
8	12	6.538058418025983
8	13	6.821028205450158
8	14	5.984895772842965
8	15	6.733659610064404
8	16	7.446706686291851
8	17	7.719499900883957
8	18	8.484342188189167
8	19	6.8602738729040915
8	20	6.348779523771036
8	21	6.318783025979769
8	22	6.386357981270333
8	23	6.497312542054988
8	24	6.517780334909457
8	25	5.601372443932881
8	26	4.6359638344659935
8	27	4.857198991820301
8	28	5.100807991238856
8	29	5.191236066877416
8	30	5.9799510403857425
8	31	3.4687728952528047
8	32	4.237279289352763
8	33	3.7192555740735784
8	34	4.04749134013284
8	35	3.249797911228818
8	36	3.979036490560727
8	37	4.498445682555104
8	38	4.2822470519801215
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115	19	2.132499700288907
115	20	1.19822793389433
115	21	4.203922545772116
115	22	5.128408597753133
115	23	4.319668155293147
115	24	4.820668070146315
115	25	4.278692366061364
115	26	4.214970972687926
115	27	3.9655701793608564
115	28	4.110866394872861
115	29	4.346654626254441
115	30	4.798250845706967
115	31	2.9926210458043982
115	32	3.1792001199822266
115	33	2.9888856843915548
115	34	3.6488865380562707
115	35	2.8216668275169425
115	36	4.739906055778118
115	37	4.551171185475287
115	38	4.55885315191523
115	39	6.725000823708843
115	40	8.367620526879577
115	41	8.791336246613042
115	42	7.895495958806883
115	43	5.072327483572252
115	44	-0.32697732966390447
115	45	6.550974325748683
115	46	3.0631029672415515
115	47	-6.934151642285681
115	48	2.1235896996418404
115	49	-2.4349615590685296
115	50	3.520119981236114
115	51	3.779966727534852
115	52	4.069019737736173
115	53	3.9946084487308084
115	54	3.4271989729093626
115	55	3.7896542518073915
115	56	5.24765085684054
116	1	3.2076770678652764
116	2	5.874037243881695
116	3	4.594792037662508
116	4	6.806028188930981
116	5	5.55968818100326
116	6	7.534054766289575
116	7	4.43851240416582
116	8	4.456796869682655
116	9	4.352758550260724
116	10	5.883321413937054
116	11	3.8486396147433233
116	12	3.359886776234937
116	13	4.630063386686832
116	14	4.593504514847229
116	15	-2.6454715199995604
116	16	3.764936963477357
116	17	5.459105444593977
116	18	4.295776090108141
116	19	4.921707774823066
116	20	4.395821101596198
116	21	5.141810744529877
116	22	5.790675678498477
116	23	4.940009770199975
116	24	5.378121486295381
116	25	5.013552614508569
116	26	4.620638674551277
116	27	4.667433533398121
116	28	4.662108630897709
116	29	4.419247793956309
116	30	4.73808003825355
116	31	3.8718912321398022
116	32	3.5192622317834648
116	33	4.530422951472296
116	34	4.1170542399196215
116	35	3.1489183803119687
116	36	5.3451079191321575
116	37	5.254975533301373
116	38	5.9233717854556005
116	39	6.523913867187101
116	40	8.494173321910637
116	41	7.795218234190681
116	42	5.953725772471483
116	43	-0.18155450400538697
116	44	-0.8296947209682521
116	45	4.879390474817008
116	46	-0.11313926878329283
116	47	-7.424446673341581
116	48	-1.2514103003581596
116	49	-4.665163422422566
116	50	-0.48259741006823376
116	51	3.1619543051746035
116	52	3.234392408543627
116	53	3.16774509469354
116	54	3.0836430722882433
116	55	3.267526922614845
116	56	5.215430359946129
117	1	2.409161577331716
117	2	4.309170634587375
117	3	3.8652309361134556
117	4	5.336686536607402
117	5	4.516336201657303
117	6	6.226825850626927
117	7	3.3148032819627247
117	8	3.109981034915009
117	9	3.2861707705705356
117	10	4.124392842508485
117	11	0.6015440897863478
117	12	-4.556528886415666
117	13	-4.758813549629863
117	14	-0.6189524558928774
117	15	-8.335122982994399
117	16	-9.097799697452079
117	17	-0.5621940390549014
117	18	-3.0063968875166402
117	19	0.12254684539105654
117	20	-0.6645274354089672
117	21	4.446457904598724
117	22	5.026260876433067
117	23	4.1050269819039364
117	24	5.074334911424469
117	25	4.8047531308596945
117	26	4.378276368182949
117	27	4.011667612571959
117	28	3.872521711792718
117	29	3.5622125099631887
117	30	5.090812396256993
117	31	3.0370160169934977
117	32	3.7787286689607456
117	33	3.7775184764292717
117	34	3.432673861262135
117	35	4.016926125578756
117	36	5.105542514657113
117	37	4.90934300318089
117	38	5.447145451548543
117	39	7.064576517789508
117	40	9.482340275439036
117	41	7.8883765818670994
117	42	6.830016650268384
117	43	-4.42531526132036
117	44	-2.1331585763899383
117	45	6.769020423181896
117	46	-1.3069860846180603
117	47	-1.361731527042096
117	48	-0.8824343967437009
117	49	-1.2283088613210182
117	50	1.0753845176426111
117	51	5.699820053883727
117	52	6.488480188233815
117	53	5.237667642025727
117	54	6.373767857141946
117	55	6.8356207264014195
117	56	8.761794387484855
118	1	2.3235038780414
118	2	5.076458965995194
118	3	4.073021000480516
118	4	5.723714098911415
118	5	4.2435189050737065
118	6	6.677890382727934
118	7	3.7680133826394027
118	8	3.735661840328447
118	9	3.60899631934096
118	10	5.083296952097527
118	11	-0.07051400560892773
118	12	-4.204325552532186
118	13	-4.569178296287728
118	14	-8.180101160299543
118	15	-10.442071324304845
118	16	-10.490933878009926
118	17	-1.478516864603673
118	18	-7.284825671144301
118	19	-1.6554693288951299
118	20	-1.4205927927286588
118	21	3.773904677993677
118	22	4.764501510201018
118	23	4.0449510617851026
118	24	4.551311310170139
118	25	3.970622086132842
118	26	3.390819692163806
118	27	3.7672378386818828
118	28	4.060836615242128
118	29	4.762080474973921
118	30	4.663349118488384
118	31	3.669958746816903
118	32	3.668479452918497
118	33	3.9701245170300297
118	34	3.8589818328746155
118	35	3.8611248382376075
118	36	5.492735120697713
118	37	5.034941286726028
118	38	6.809746540837203
118	39	7.724256332940524
118	40	9.373576453026097
118	41	8.741157568828648
118	42	7.420213052314928
118	43	0.37250616135665027
118	44	2.203695689990653
118	45	7.293529418065539
118	46	-0.017004239429084578
118	47	-0.82500929565078
118	48	0.9934527133404707
118	49	1.4491956773817378
118	50	2.6261354666440937
118	51	6.779371134920201
118	52	7.220449160011338
118	53	5.371022981190606
118	54	7.0889023677872665
118	55	7.269850797370225
118	56	11.165161279711295
119	1	2.700371873060085
119	2	5.871602178946631
119	3	4.602908920779392
119	4	6.704567149969947
119	5	4.703357012172091
119	6	7.6817820390168485
119	7	4.081369547022966
119	8	4.0152384281242135
119	9	3.743180628182806
119	10	5.4312110243266645
119	11	0.5734772770809862
119	12	-6.611704132855973
119	13	-2.0136054444819997
119	14	-5.295294186451475
119	15	-8.66576372779177
119	16	-8.377920179379789
119	17	-0.15940104891251394
119	18	-4.907957676125623
119	19	-0.5774805368652416
119	20	-1.4361594178843227
119	21	4.503823731542866
119	22	5.5666497044725
119	23	4.5617630169532255
119	24	3.9219526551265496
119	25	4.8098188482748085
119	26	4.804891921304527
119	27	4.209641325605919
119	28	4.220550189339267
119	29	4.973630910839425
119	30	5.234021596695108
119	31	3.6097159074644765
119	32	3.654002491523727
119	33	4.169221652771
119	34	4.136534759400146
119	35	4.172457341350935
119	36	5.104036490560727
119	37	5.669748260574099
119	38	6.086521136104949
119	39	7.069368412641648
119	40	8.328588906326218
119	41	5.585802649775097
119	42	4.7784010971468085
119	43	-3.8585025559534394
119	44	-3.187649266422799
119	45	4.869650215076746
119	46	-1.5011262817703042
119	47	-2.993440179835087
119	48	-5.365046663994523
119	49	-3.6968192665784123
119	50	0.045811680840856184
119	51	4.973642616862914
119	52	4.895106694257912
119	53	5.37553730248575
119	54	3.582831383976554
119	55	5.4680139356018564
119	56	7.105852437868208
120	1	2.758272305960517
120	2	5.853203910548359
120	3	4.493601561472033
120	4	6.356623427026221
120	5	4.74123580005088
120	6	7.593578575813385
120	7	3.7658933565467763
120	8	3.8615587744445605
120	9	3.8914490264511983
120	10	4.672011890127536
120	11	-1.1186222900185818
120	12	-5.2085656047174425
120	13	-3.7360080418845953
120	14	-3.879709770867059
120	15	-0.6782096152376589
120	16	-6.544586846046457
120	17	0.44124830173683804
120	18	-2.9810096241775703
120	19	0.6330172986325913
120	20	0.13094014921524177
120	21	3.2489536016727385
120	22	5.046628059450857
120	23	4.041200246390456
120	24	3.2471691053430014
120	25	3.8081954716514304
120	26	4.641472007884612
120	27	3.7983859143505008
120	28	3.8763943451834244
120	29	4.672223984432499
120	30	4.651770514444024
120	31	4.047486470235036
120	32	3.7097384222596546
120	33	3.973875332424676
120	34	3.777768525633906
120	35	4.354275523169115
120	36	5.181417442941676
120	37	5.216285057110898
120	38	5.604919404503221
120	39	6.994151962425196
120	40	7.54774475048206
120	41	-6.508353194380749
120	42	-5.040321846576138
120	43	-8.163697361148245
120	44	-15.142194720968252
120	45	-4.29322857280204
120	46	-7.630996411640435
120	47	-7.284565720960629
120	48	-4.99545791940578
120	49	-14.257425327184471
120	50	-4.533192648163471
120	51	0.40004954326984077
120	52	-0.6882266390754204
120	53	0.007030808979255454
120	54	0.0509049770501484
120	55	0.5681221607100824
120	56	3.676739883755655
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2901.0
5	196.0
6	54.0
7	148.0
8	142.0
9	177.0
10	196.0
11	210.0
12	173.0
13	211.0
14	281.0
15	347.0
16	433.0
17	562.0
18	702.0
19	921.0
20	1185.0
21	1663.0
22	1983.0
23	2260.0
24	2580.0
25	2871.0
26	3025.0
27	3083.0
28	3296.0
29	3351.0
30	3878.0
31	4274.0
32	4796.0
33	5332.0
34	5886.0
35	6528.0
36	7240.0
37	7835.0
38	7391.0
39	6546.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.65032323515763	0.2665745707286012	0.057200211532857745	0.02590198258091671
8	99.55750779757601	0.3378050228261222	0.07554744919434042	0.0291397304035313
9	98.67895611630367	0.4079694346709262	0.658363373410754	0.25471107561465234
10	47.481571818642955	18.899813289875564	25.49078860744466	8.127826284036823
11	31.01654489137356	27.095632278187292	14.277388648456135	27.610434181983013
12	26.945616629072816	25.90306183019092	16.97982883106511	30.171492709671156
13	20.353562062229514	13.834896446032142	20.43666425634329	45.37487723539506
14	19.630465048512256	30.89027272629159	28.321659453684017	21.157602771512135
15	39.89241714384869	16.171048065243795	21.408120770432067	22.528414020475445
16	30.99043258536164	29.60067382243051	20.22763103902555	19.1812625531823
17	24.111499762490823	17.34464740683163	39.956600595932116	18.58725223474543
18	21.85803555047109	16.751027984933682	27.124772008590824	34.266164456004404
19	29.088573155985493	19.189410951805147	18.475773017792367	33.246242874417
20	22.24035447962823	26.228250297200905	32.49432616448719	19.03706905868367
21	21.124146044011784	18.801601605922922	27.544599976256517	32.52965237380877
22	19.58945357609247	17.02623654985592	35.44578391271032	27.93852596134129
23	20.31470908835814	19.222508822862817	32.421727446388296	28.04105464239075
24	20.22320320341928	19.74614413228136	26.698038877076343	33.33261378722302
25	28.608589592755294	19.566742582058783	30.960527593985777	20.864140231200146
26	35.848873243999094	18.901642353500126	18.587424819945795	26.66205958255499
27	20.22119974942216	31.11053507009699	18.368867863391873	30.299397317088978
28	27.53254041919399	32.6375547736741	18.33380102316144	21.496103783970472
29	37.53432358219282	26.062139196990337	17.61259216016951	18.79094506064734
30	29.661062701780526	19.163616338958896	32.808568991394296	18.366751967866282
31	31.304713895575393	30.858906963439516	20.144428492783973	17.69195064820112
32	38.59249503411348	24.695569565592884	19.094913204939978	17.617022195353655
33	31.626203364558688	24.263935258716625	19.47748857411429	24.632372802610398
34	21.6746318361698	25.48926323984171	34.71768700127587	18.118417922712627
35	21.325365004917167	22.27313498967936	40.057060724282145	16.34443928112133
36	28.327232282656816	27.49036249959506	26.107097735592344	18.075307482155775
37	29.269478940371584	20.144180365963056	32.223339061638725	18.36300163202663
38	35.42365182028597	21.300482381189298	24.78314477925112	18.492721019273617
39	29.07974664389632	20.300914416654056	18.832011068116476	31.787327871333147
40	24.089830914194938	16.237242399843318	29.409396557352075	30.263530128609666
41	20.615971654401744	14.625238484600708	26.599073316980103	38.15971654401744
42	26.038769170824626	25.68701092074075	22.42918835842542	25.8450315500092
43	18.000952545895395	22.372921718046417	33.418124350536885	26.2080013855213
44	19.453688146983904	25.295105956316732	26.278250501327843	28.972955395371525
45	26.26729107407447	19.774823979840146	32.72082283341084	21.237062112674533
46	18.7360602845326	26.936402416578247	22.299213962452093	32.02832333643706
47	16.680223415216094	27.77398188818394	22.68532075267068	32.860473943929286
48	25.941526531229414	26.596031926038734	22.324466189288145	25.137975353443714
49	19.745057513553288	29.26211680174867	20.420287189031846	30.572538495666198
50	25.718240108993008	25.57551117502676	22.821491517359945	25.88475719862029
51	19.546168426753315	31.712970484700786	23.50284321689683	25.238017871649067
52	19.555791044453336	31.45578996312676	29.217443960250435	19.770975032169467
53	20.05324732951655	29.03169947726707	29.483003062803707	21.432050130412666
54	19.924030907083957	26.38789688981235	27.67460987381772	26.013462329285975
55	19.65099537763729	26.19807960856057	28.413999155633977	25.736925858168156
56	25.250481487091818	20.77211053645236	28.92493129341499	25.052476683040837
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	3.5
5	6.0
6	4.0
7	2.0
8	15.0
9	28.0
10	52.0
11	76.0
12	113.0
13	156.0
14	162.0
15	158.5
16	155.0
17	160.0
18	165.0
19	179.0
20	193.0
21	203.0
22	222.5
23	232.0
24	283.5
25	335.0
26	391.0
27	447.0
28	443.5
29	494.5
30	549.0
31	627.5
32	706.0
33	867.0
34	1028.0
35	1123.5
36	1219.0
37	1248.5
38	1450.5
39	1623.0
40	1977.5
41	2332.0
42	3218.0
43	4104.0
44	7066.0
45	10028.0
46	9803.5
47	7771.0
48	5963.0
49	5510.0
50	5057.0
51	5316.5
52	5576.0
53	5489.5
54	5130.5
55	4858.0
56	5043.5
57	5229.0
58	5470.5
59	5712.0
60	5198.5
61	4685.0
62	4315.5
63	3628.0
64	3310.0
65	2783.5
66	2257.0
67	2063.0
68	1869.0
69	1611.0
70	1353.0
71	1193.5
72	786.0
73	538.0
74	400.5
75	263.0
76	240.0
77	217.0
78	226.5
79	156.5
80	77.0
81	48.0
82	19.0
83	10.5
84	2.0
85	2.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.003237747822614589
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.4845829241179835
16	0.05504171298444802
17	0.03129822895194103
18	0.0
19	0.03561522604876048
20	0.1370646578240176
21	0.0
22	0.0
23	0.0
24	0.006475495645229178
25	0.01079249274204863
26	0.0496454666134237
27	0.07662669846854528
28	0.003237747822614589
29	0.1672836375017538
30	0.04748696806501398
31	0.01726798838727781
32	0.026981231855121576
33	0.11224192451730576
34	0.1845516258890316
35	0.13382691000140304
36	0.05396246371024315
37	0.14461940274345164
38	0.21477060556676775
39	0.15001564911447599
40	0.8105162049278521
41	1.0058603235589323
42	0.28492180839008385
43	0.2946350518579276
44	0.433858208230355
45	0.21153285774415317
46	0.31837853589043463
47	0.48782067194059814
48	0.07338895064593069
49	0.2633368229059866
50	0.1877893737116462
51	0.35831075903601456
52	0.19210637080846563
53	0.2784463127448547
54	0.2708915678254206
55	0.3021897967773617
56	0.25362357943814284
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	92657.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.05714624906915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.35932315859323	57.484054091973626
2	6.566025215660252	8.543337254605696
3	1.944260119442601	3.794640448104299
4	0.8709356337093563	2.2664234758302126
5	0.5009953550099535	1.6296664040493434
6	0.3550099535500995	1.3857560680790444
7	0.232249502322495	1.057664288720766
8	0.16921035169210352	0.8806674077511684
9	0.145985401459854	0.8547654251702517
>10	0.7714001327140013	9.082961891708127
>50	0.041473125414731256	1.8724974907454375
>100	0.0364963503649635	4.586809415370668
>500	0.00165892501658925	0.7349687557335118
>1k	0.004976775049767751	5.825787582157851
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	2526	2.726183666641484	No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	1576	1.7008968561468643	No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	1296	1.3987070593695026	No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	681	0.7349687557335118	No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	469	0.5061679096020808	No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	386	0.4165902198430772	No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	342	0.3691032517780632	No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCATTAGTTGGTCTTCCA	309	0.3334880257293027	No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	298	0.3216162837130492	No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	248	0.2676538200028061	No Hit
TAGAGAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	212	0.22880084613143098	No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	196	0.21153285774415317	No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	193	0.2082951099215386	No Hit
TAGAGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	167	0.18023462879221214	No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	142	0.15325339693709056	No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	137	0.14785715056606624	No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	132	0.14246090419504193	No Hit
TAGAGAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	131	0.14138165492083707	No Hit
TAGAGAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	128	0.13814390709822247	No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	121	0.13058916217878844	No Hit
TAGAGAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	111	0.11979666943673979	No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCCTTAGTTGGTCTTCCA	108	0.1165589216141252	No Hit
TAGAGAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC	107	0.11547967233992035	No Hit
TAGAGAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	107	0.11547967233992035	No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	104	0.11224192451730576	No Hit
TAGAGAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	102	0.11008342596889605	No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC	97	0.10468717959787172	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.001079249274204863	0.0	0.0	0.0	0.0
5	0.001079249274204863	0.0	0.0	0.0	0.0
6	0.010792492742048632	0.0	0.0	0.0	0.0
7	0.010792492742048632	0.0	0.0	0.0	0.0
8	0.011871742016253494	0.0	0.0	0.0	0.0
9	0.012950991290458357	0.0	0.0	0.0	0.0
10	0.01403024056466322	0.0	0.0	0.0	0.0
11	0.01726798838727781	0.0	0.0	0.0	0.0
12	0.021584985484097263	0.0	0.0	0.0	0.0
13	0.022664234758302124	0.0	0.0	0.0	0.0
14	0.04856621733921884	0.0	0.0	0.0	0.0
15	0.050724715887628564	0.0	0.0	0.0	0.0
16	0.05180396516183343	0.0	0.0	0.0	0.0
17	0.05396246371024316	0.0	0.0	0.0	0.0
18	0.057200211532857745	0.0	0.0	0.0	0.0
19	0.05935871008126747	0.0	0.0	0.0	0.0
20	0.061517208629677196	0.0	0.0	0.0	0.0
21	0.061517208629677196	0.0	0.0	0.0	0.0
22	0.06259645790388206	0.0	0.0	0.0	0.0
23	0.06583420572649665	0.0	0.0	0.0	0.0
24	0.07123045209752096	0.0	0.0	0.0	0.0
25	0.07338895064593069	0.0	0.0	0.0	0.0
26	0.07770594774275014	0.0	0.0	0.0	0.0
27	0.08526069266218418	0.0	0.0	0.0	0.0
28	0.09173618830741337	0.0	0.0	0.0	0.0
29	0.09605318540423281	0.0	0.0	0.0	0.0
30	0.0992909332268474	0.0	0.0	0.0	0.0
31	0.11008342596889603	0.0	0.0	0.0	0.0
32	0.11763817088833008	0.0	0.0	0.0	0.0
33	0.11871742016253493	0.0	0.0	0.0	0.0
34	0.12195516798514953	0.0	0.0	0.0	0.0
35	0.12303441725935439	0.0	0.0	0.0	0.0
36	0.12519291580776412	0.0	0.0	0.0	0.0
37	0.12735141435617384	0.0	0.0	0.0	0.0
38	0.12843066363037872	0.0	0.0	0.0	0.0
39	0.12950991290458358	0.0	0.0	0.0	0.0
40	0.13058916217878844	0.0	0.0	0.0	0.0
41	0.13814390709822247	0.0	0.0	0.0	0.0
42	0.14461940274345164	0.0	0.0	0.0	0.0
43	0.15649114475970516	0.0	0.0	0.0	0.0
44	0.17915537951800728	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCAATC	20	4.00145E-4	50.404152	40
CGCATTG	85	0.0	50.404152	39
AATTGGA	20	4.00145E-4	50.404152	39
TAATTGG	20	4.00145E-4	50.404152	38
GCTCAAT	20	4.00145E-4	50.404152	39
CGTTGTA	15	0.008143122	50.40415	39
GTCTAAT	15	0.008143122	50.40415	39
TCAATTC	15	0.008143122	50.40415	41
TAATTAT	15	0.008143122	50.40415	40
TCGTCTC	115	0.0	50.40415	40
ACGTCTC	15	0.008143122	50.40415	41
GCTCGTC	120	0.0	50.40415	38
TCACGTC	15	0.008143122	50.40415	39
GGCGCAT	90	0.0	50.37664	37
TTGTCCA	105	0.0	50.376637	37
CGCCGCT	15	0.008160729	50.376633	37
ATGCTCG	125	0.0	50.266888	36
AGCGTCA	15	0.00823144	50.266884	35
ATGCCCG	15	0.00823144	50.266884	36
ATGAGTC	20	4.066636E-4	50.239525	46
>>END_MODULE