FastQCFastQC Report
Fri 17 Jun 2016
SRR1525905_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525905_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5961
Sequences flagged as poor quality0
Sequence length40
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT210.35228988424760943No Hit
TAGAGAGGGATGAGATGGACTTAAATGAATTGACGGGTAC150.25163563160543534No Hit
TAGAGAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC150.25163563160543534No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC120.20130850528434827No Hit
TAGAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT110.18453279651065257No Hit
TAGAGAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC110.18453279651065257No Hit
TAGAGAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC110.18453279651065257No Hit
TAGAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT110.18453279651065257No Hit
TAGAGAGGGATGAGATGGACTCAAATGAATTGACGGGTAC100.16775708773695688No Hit
TAGAGAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC100.16775708773695688No Hit
TAGAGAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA100.16775708773695688No Hit
TAGAGAGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC100.16775708773695688No Hit
TAGAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT90.1509813789632612No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG80.1342056701895655No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT80.1342056701895655No Hit
TAGAGAGGGCAGAGTCGATTTTTTTTTTTTTTTTTTTTTT80.1342056701895655No Hit
TAGAGAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC80.1342056701895655No Hit
TAGAGAGGGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTT70.11742996141586982No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG70.11742996141586982No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG60.10065425264217413No Hit
TAGAGAGGGCTCGTCTTACTCAGCAACATCTTTTGATACA60.10065425264217413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGCT200.002572934634.0000046
GAAGCAG200.002572934634.0000049
AGGGCAG200.002572934634.0000046
GAGGGTG200.002572934634.0000045
GAGGGCA406.019036E-834.0000045
GGGGAGT200.002572934634.0000047
AGGGGGG552.0008883E-1134.06
AGAGGGT552.0008883E-1134.04
AGGGATA301.251807E-534.06
AGGGACA251.7967274E-434.06
AGGGAAG502.8376235E-1034.06
GGAAGCA251.7967274E-434.08
GAGGGGG850.034.05
GAGGGGC301.251807E-534.05
GAGGGCC251.7967274E-434.05
GAGGGAT1050.034.05
GAGGGAC454.147296E-934.05
GAGGGAA601.8189894E-1234.05
TAGAGAG5900.033.423731
AGAGAGG5800.033.413792
Produced by FastQC (version 0.11.5)