##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525905_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5961
Sequences flagged as poor quality	0
Sequence length	40
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.008723368562322	33.0	30.0	36.0	4.0	38.0
2	18.911088743499413	19.0	10.0	28.0	4.0	33.0
3	21.228988424760946	23.0	11.0	31.0	4.0	35.0
4	21.54403623553095	24.0	10.0	33.0	4.0	36.0
5	23.140412682435834	27.0	10.0	35.0	4.0	37.0
6	21.535145109880894	24.0	9.0	33.0	4.0	36.0
7	24.802382150645865	30.0	16.0	35.0	4.0	37.0
8	23.284180506626406	28.0	10.0	33.0	4.0	36.0
9	24.734105015936922	30.0	15.0	35.0	4.0	37.0
10	18.294581446066097	18.0	6.0	28.0	4.0	34.0
11	22.417547391377287	27.0	7.0	33.0	4.0	36.0
12	22.81043449085724	27.0	4.0	33.0	4.0	36.0
13	23.28283844992451	29.0	4.0	34.0	4.0	37.0
14	23.72269753397081	30.0	4.0	35.0	4.0	37.0
15	21.425599731588658	25.0	4.0	33.0	4.0	36.0
16	19.165744002684114	21.0	4.0	31.0	4.0	35.0
17	20.065425264217414	24.0	4.0	32.0	4.0	35.0
18	19.499245093105184	23.0	4.0	32.0	4.0	35.0
19	16.993289716490523	16.0	4.0	29.0	4.0	34.0
20	18.554269417882907	20.0	4.0	31.0	4.0	35.0
21	15.651400771682603	12.0	4.0	27.0	4.0	33.0
22	16.18788793826539	12.0	4.0	29.0	4.0	35.0
23	16.0385841301795	12.0	4.0	29.0	4.0	34.0
24	15.68847508807247	10.0	4.0	29.0	4.0	33.0
25	15.27344405301124	6.0	4.0	28.0	4.0	33.0
26	13.8225130011743	4.0	4.0	26.0	4.0	33.0
27	13.821841972823352	4.0	4.0	26.0	4.0	33.0
28	13.595034390202986	4.0	4.0	26.0	4.0	33.0
29	12.892299949672873	4.0	4.0	24.0	4.0	33.0
30	12.088072471061903	4.0	4.0	22.0	4.0	32.0
31	12.507297433316557	4.0	4.0	25.0	4.0	33.0
32	11.776547559134373	4.0	4.0	22.0	4.0	32.0
33	11.90907565844657	4.0	4.0	23.0	4.0	32.0
34	11.77403120281832	4.0	4.0	23.0	4.0	32.0
35	11.396074484146956	4.0	4.0	21.0	4.0	32.0
36	12.005535983895319	4.0	4.0	24.0	4.0	35.0
37	11.972487837611139	4.0	4.0	24.0	4.0	35.0
38	11.828720013420567	4.0	4.0	24.0	4.0	35.0
39	11.5324609964771	4.0	4.0	21.0	4.0	35.0
40	11.422076832746184	4.0	4.0	21.0	4.0	34.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-12.110637245107824
1	2	-13.805225007019125
1	3	-15.886178892186248
1	4	-15.432153336859214
1	5	-16.35571238810209
1	6	-14.82459105926753
1	7	-17.840233454454047
1	8	-16.81482928917488
1	9	-17.801210794924035
1	10	-12.106226006836305
1	11	-15.336750868412633
1	12	-15.612892320914376
1	13	-16.73910537501714
1	14	-16.563778020182436
1	15	-13.641364999379704
1	16	-12.410583445965797
1	17	-14.014837800271625
1	18	-12.925622713071242
1	19	-11.670480676186555
1	20	-12.31302673715174
1	21	-9.052370853003207
1	22	-10.00809212764359
1	23	-10.277530378934799
1	24	-9.82074952824953
1	25	-8.850296607803955
1	26	-8.168965242274066
1	27	-6.649293099873981
1	28	-6.967359419991772
1	29	-6.658250960640664
1	30	-5.539610864625573
1	31	-6.360480614973259
1	32	-5.419462426462428
1	33	-5.623183202418498
1	34	-4.879056531703588
1	35	-4.407348857841507
1	36	-5.257585418666304
1	37	-5.783884811267164
1	38	-5.056109143307674
1	39	-4.494855348899469
1	40	-4.3984146833558615
2	1	7.611584977114397
2	2	16.861441659647543
2	3	14.89159888559153
2	4	15.123402218696341
2	5	13.033176500786798
2	6	-0.9357021703786401
2	7	-18.56245567667627
2	8	-17.42594040028599
2	9	-18.634544128257367
2	10	-12.439559340169637
2	11	-16.11452864619041
2	12	-16.390670098692155
2	13	-17.016883152794918
2	14	-17.286000242404658
2	15	-14.863587221601925
2	16	-13.466139001521352
2	17	-14.403726689160514
2	18	-13.536733824182352
2	19	-11.781591787297666
2	20	-12.868582292707295
2	21	-10.330148630780986
2	22	-10.1192032387547
2	23	-10.666419267823688
2	24	-10.32074952824953
2	25	-9.461407718915066
2	26	-8.335631908940732
2	27	-8.093737544318426
2	28	-7.74513719776955
2	29	-6.991584293973997
2	30	-6.928499753514462
2	31	-7.249369503862148
2	32	-6.697240204240206
2	33	-6.900960980196276
2	34	-6.490167642814699
2	35	-6.018459968952618
2	36	-6.757585418666304
2	37	-6.672773700156053
2	38	-6.556109143307674
2	39	-5.994855348899469
2	40	-5.8984146833558615
3	1	-10.388415022885603
3	2	-14.138558340352457
3	3	-16.10840111440847
3	4	-15.876597781303659
3	5	-16.966823499213202
3	6	-15.43570217037864
3	7	-18.56245567667627
3	8	-17.42594040028599
3	9	-18.634544128257367
3	10	-12.439559340169637
3	11	-16.11452864619041
3	12	-16.390670098692155
3	13	-17.016883152794918
3	14	-17.286000242404658
3	15	-14.863587221601925
3	16	-13.466139001521352
3	17	-14.403726689160514
3	18	-13.536733824182352
3	19	-11.781591787297666
3	20	-12.868582292707295
3	21	-10.330148630780986
3	22	-10.1192032387547
3	23	-10.666419267823688
3	24	-10.32074952824953
3	25	-9.461407718915066
3	26	-8.335631908940732
3	27	-8.093737544318426
3	28	-7.74513719776955
3	29	-6.991584293973997
3	30	-6.928499753514462
3	31	-7.249369503862148
3	32	-6.697240204240206
3	33	-6.900960980196276
3	34	-6.490167642814699
3	35	-6.018459968952618
3	36	-6.757585418666304
3	37	-6.672773700156053
3	38	-6.556109143307674
3	39	-5.994855348899469
3	40	-5.8984146833558615
6	1	5.278251643781061
6	2	6.528108326314211
6	3	3.391598885591531
6	4	-1.3765977813036585
6	5	-0.30015683254653425
6	6	4.230964496288028
6	7	2.604210989990399
6	8	-2.9259404002859917
6	9	2.6987892050759648
6	10	8.060440659830363
6	11	3.3854713538095886
6	12	6.109329901307845
6	13	4.816450180538414
6	14	5.38066642426201
6	15	4.636412778398075
6	16	3.86719433181198
6	17	4.096273310839486
6	18	1.6299328424843154
6	19	6.385074879369002
6	20	6.464751040626037
6	21	3.336518035885682
6	22	11.547463427911968
6	23	13.333580732176312
6	24	10.012583805083802
6	25	6.8719256144182665
6	26	3.164368091059268
6	27	1.739595789014908
6	28	5.588196135563782
6	29	7.508415706026003
6	30	6.738166913152206
6	31	7.083963829471184
6	32	3.136093129093128
6	33	5.9323723531370565
6	34	4.676499023851967
6	35	0.8148733643807162
6	36	1.57574791466703
6	37	2.1605596331772805
6	38	1.1105575233589917
6	39	1.5051446511005313
6	40	3.9349186499774724
7	1	7.1830135485429665
7	2	11.29001308821897
7	3	8.653503647496294
7	4	9.123402218696341
7	5	10.890319357929656
7	6	11.659535924859455
7	7	9.532782418561826
7	8	9.859773885428293
7	9	9.365455871742633
7	10	9.655678755068458
7	11	8.409280877619114
7	12	7.752187044164987
7	13	10.078354942443177
7	14	6.428285471881058
7	15	8.23165087363617
7	16	9.771956236573885
7	17	10.596273310839486
7	18	6.987075699627173
7	19	8.646979641273761
7	20	11.2266558025308
7	21	9.669851369219014
7	22	11.8807967612453
7	23	12.095485494081075
7	24	10.917345709845707
7	25	11.014782757275409
7	26	4.045320472011648
7	27	3.096738646157764
7	28	5.63581518318283
7	29	3.627463325073622
7	30	2.976262151247443
7	31	1.2744400199473755
7	32	0.39799789099788896
7	33	-0.5676276468629418
7	34	1.4145942619472063
7	35	-0.5898885403811889
7	36	0.19479553371464853
7	37	-1.1965832239655771
7	38	-0.9846805718791032
7	39	-2.8519982060423255
7	40	-2.184128969070147
9	1	5.611584977114397
9	2	-4.138558340352457
9	3	2.891598885591531
9	4	10.623402218696341
9	5	6.033176500786798
9	6	2.56429782962136
9	7	11.937544323323731
9	8	12.574059599714008
9	9	9.365455871742633
9	10	13.060440659830363
9	11	9.385471353809589
9	12	7.109329901307845
9	13	11.983116847205082
9	14	1.7139997575953423
9	15	2.136412778398075
9	16	13.533860998478648
9	17	13.596273310839486
9	18	6.9632661758176475
9	19	-0.7815917872976659
9	20	12.131417707292705
9	21	8.669851369219014
9	22	-3.6192032387547
9	23	13.333580732176312
9	24	8.67925047175047
9	25	12.538592281084934
9	26	7.664368091059268
9	27	3.406262455681574
9	28	6.75486280223045
9	29	1.5084157060260033
9	30	2.071500246485538
9	31	2.250630496137852
9	32	16.802759795759794
9	33	17.599039019803726
9	34	0.009832357185301177
9	35	5.481540031047382
9	36	8.742414581333696
9	37	8.827226299843947
9	38	-1.0561091433076744
9	39	-0.4948553488994687
9	40	-0.3984146833558615
10	1	8.236584977114397
10	2	8.423941659647543
10	3	13.01659888559153
10	4	13.498402218696341
10	5	13.470676500786798
10	6	14.43929782962136
10	7	12.312544323323731
10	8	13.324059599714008
10	9	11.802955871742633
10	10	11.185440659830363
10	11	7.822971353809589
10	12	11.984329901307845
10	13	11.795616847205082
10	14	13.151499757595342
10	15	9.573912778398075
10	16	16.033860998478648
10	17	14.658773310839486
10	18	16.025766175817648
10	19	14.968408212702334
10	20	14.381417707292705
10	21	17.919851369219014
10	22	21.5057967612453
10	23	19.958580732176312
10	24	20.30425047175047
10	25	17.351092281084934
10	26	12.101868091059268
10	27	13.531262455681574
10	28	9.37986280223045
10	29	9.633415706026003
10	30	5.509000246485538
10	31	6.500630496137852
10	32	2.990259795759794
10	33	6.536539019803724
10	34	4.197332357185301
10	35	1.6690400310473823
10	36	-1.695085418666304
10	37	-0.11027370015605342
10	38	1.1313908566923256
10	39	-0.4948553488994687
10	40	1.6640853166441385
12	1	7.111584977114397
12	2	6.361441659647543
12	3	5.891598885591531
12	4	6.1234022186963415
12	5	9.033176500786798
12	6	9.06429782962136
12	7	11.437544323323731
12	8	11.574059599714008
12	9	11.365455871742633
12	10	11.060440659830363
12	11	13.885471353809589
12	12	17.109329901307845
12	13	16.483116847205082
12	14	15.213999757595342
12	15	18.636412778398075
12	16	16.533860998478648
12	17	14.596273310839486
12	18	18.963266175817648
12	19	16.718408212702336
12	20	19.631417707292705
12	21	17.669851369219014
12	22	17.3807967612453
12	23	14.833580732176312
12	24	17.67925047175047
12	25	17.538592281084934
12	26	13.164368091059268
12	27	3.906262455681574
12	28	12.75486280223045
12	29	2.5084157060260033
12	30	2.571500246485538
12	31	-0.24936950386214818
12	32	-1.697240204240206
12	33	1.0990390198037243
12	34	-4.490167642814699
12	35	-0.5184599689526177
12	36	6.742414581333696
12	37	2.3272262998439466
12	38	6.943890856692326
12	39	4.505144651100531
12	40	6.1015853166441385
13	1	-12.788415022885603
13	2	-12.238558340352457
13	3	-12.50840111440847
13	4	-13.876597781303659
13	5	-13.166823499213201
13	6	-12.83570217037864
13	7	-12.662455676676268
13	8	-11.725940400285992
13	9	-12.634544128257367
13	10	-6.239559340169638
13	11	-6.614528646190411
13	12	-7.490670098692155
13	13	-8.216883152794917
13	14	-8.086000242404658
13	15	-4.163587221601926
13	16	-3.6661390015213513
13	17	-3.9037266891605142
13	18	-5.436733824182353
13	19	-2.481591787297665
13	20	-3.268582292707295
13	21	-2.6301486307809867
13	22	-2.8192032387546995
13	23	-4.466419267823689
13	24	-4.52074952824953
13	25	-4.561407718915065
13	26	-6.035631908940732
13	27	-6.993737544318426
13	28	-5.94513719776955
13	29	-4.2915842939739965
13	30	-5.728499753514462
13	31	-5.549369503862148
13	32	-6.297240204240206
13	33	-6.400960980196276
13	34	-5.990167642814699
13	35	-5.418459968952618
13	36	-3.957585418666304
13	37	-4.472773700156053
13	38	-3.7561091433076745
13	39	-3.194855348899469
13	40	-3.0984146833558617
14	1	8.611584977114397
14	2	-0.13855834035245707
14	3	13.89159888559153
14	4	16.12340221869634
14	5	15.033176500786798
14	6	15.56429782962136
14	7	15.437544323323731
14	8	16.57405959971401
14	9	15.365455871742633
14	10	18.560440659830363
14	11	14.885471353809589
14	12	17.609329901307845
14	13	16.983116847205082
14	14	16.713999757595342
14	15	-0.8635872216019251
14	16	18.533860998478648
14	17	17.596273310839486
14	18	18.463266175817648
14	19	20.218408212702336
14	20	17.131417707292705
14	21	23.669851369219014
14	22	23.8807967612453
14	23	23.333580732176312
14	24	23.67925047175047
14	25	19.538592281084934
14	26	18.664368091059266
14	27	20.906262455681574
14	28	-7.74513719776955
14	29	-6.991584293973997
14	30	-6.928499753514462
14	31	-7.249369503862148
14	32	-6.697240204240206
14	33	-6.900960980196276
14	34	-6.490167642814699
14	35	-6.018459968952618
14	36	-6.757585418666304
14	37	-6.672773700156053
14	38	-6.556109143307674
14	39	-5.994855348899469
14	40	-5.8984146833558615
15	1	10.611584977114397
15	2	16.861441659647543
15	3	6.891598885591531
15	4	18.12340221869634
15	5	19.033176500786798
15	6	20.56429782962136
15	7	17.43754432332373
15	8	18.57405959971401
15	9	17.365455871742633
15	10	19.560440659830363
15	11	14.885471353809589
15	12	17.609329901307845
15	13	12.983116847205082
15	14	16.713999757595342
15	15	12.136412778398075
15	16	-0.466139001521352
15	17	18.596273310839486
15	18	10.463266175817648
15	19	21.218408212702336
15	20	15.131417707292705
15	21	7.669851369219014
15	22	23.8807967612453
15	23	23.333580732176312
15	24	20.67925047175047
15	25	24.538592281084934
15	26	-8.335631908940732
15	27	-8.093737544318426
15	28	-7.74513719776955
15	29	-6.991584293973997
15	30	-6.928499753514462
15	31	-7.249369503862148
15	32	-6.697240204240206
15	33	-6.900960980196276
15	34	-6.490167642814699
15	35	-6.018459968952618
15	36	-6.757585418666304
15	37	-6.672773700156053
15	38	-6.556109143307674
15	39	-5.994855348899469
15	40	-5.8984146833558615
16	1	8.611584977114397
16	2	10.361441659647543
16	3	9.39159888559153
16	4	13.873402218696341
16	5	14.533176500786798
16	6	7.56429782962136
16	7	13.187544323323731
16	8	14.324059599714008
16	9	13.115455871742633
16	10	8.310440659830363
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109	29	10.508415706026003
109	30	10.571500246485538
109	31	9.750630496137852
109	32	8.802759795759794
109	33	10.099039019803724
109	34	9.009832357185301
109	35	9.481540031047382
109	36	8.742414581333696
109	37	5.827226299843947
109	38	8.943890856692326
109	39	-0.4948553488994687
109	40	9.601585316644139
111	1	-23.388415022885603
111	2	-14.138558340352457
111	3	-16.10840111440847
111	4	-15.876597781303659
111	5	-16.966823499213202
111	6	-15.43570217037864
111	7	-18.56245567667627
111	8	-17.42594040028599
111	9	-18.634544128257367
111	10	-12.439559340169637
111	11	-16.11452864619041
111	12	-16.390670098692155
111	13	-17.016883152794918
111	14	-17.286000242404658
111	15	-14.863587221601925
111	16	-13.466139001521352
111	17	-14.403726689160514
111	18	-13.536733824182352
111	19	-11.781591787297666
111	20	-12.868582292707295
111	21	-10.330148630780986
111	22	-10.1192032387547
111	23	-10.666419267823688
111	24	-10.32074952824953
111	25	-9.461407718915066
111	26	-8.335631908940732
111	27	-8.093737544318426
111	28	-7.74513719776955
111	29	-6.991584293973997
111	30	-6.928499753514462
111	31	-7.249369503862148
111	32	-6.697240204240206
111	33	-6.900960980196276
111	34	-6.490167642814699
111	35	-6.018459968952618
111	36	-6.757585418666304
111	37	-6.672773700156053
111	38	-6.556109143307674
111	39	-5.994855348899469
111	40	-5.8984146833558615
112	1	6.040156405685828
112	2	8.432870231076116
112	3	2.605884599877246
112	4	9.837687932982057
112	5	6.033176500786798
112	6	1.7071549724785022
112	7	8.723258609038016
112	8	2.0026310282854354
112	9	10.508313014599779
112	10	3.8461549455446473
112	11	2.4568999252381616
112	12	-0.819241527263582
112	13	-1.8740260099377757
112	14	-1.5717145281189424
112	15	-6.720730078744783
112	16	-8.037567572949923
112	17	-5.832298117731943
112	18	-6.108162395610924
112	19	-7.210163215869095
112	20	-4.297153721278724
112	21	-7.615862916495272
112	22	-6.404917524468986
112	23	-10.666419267823688
112	24	-10.32074952824953
112	25	-9.461407718915066
112	26	-8.335631908940732
112	27	-8.093737544318426
112	28	-7.74513719776955
112	29	-6.991584293973997
112	30	-6.928499753514462
112	31	-7.249369503862148
112	32	-6.697240204240206
112	33	-6.900960980196276
112	34	-6.490167642814699
112	35	-6.018459968952618
112	36	-6.757585418666304
112	37	-6.672773700156053
112	38	-6.556109143307674
112	39	-5.994855348899469
112	40	-5.8984146833558615
113	1	4.611584977114397
113	2	-2.138558340352457
113	3	10.89159888559153
113	4	-15.876597781303659
113	5	-16.966823499213202
113	6	-15.43570217037864
113	7	-18.56245567667627
113	8	-17.42594040028599
113	9	-18.634544128257367
113	10	-12.439559340169637
113	11	-16.11452864619041
113	12	-16.390670098692155
113	13	-17.016883152794918
113	14	-17.286000242404658
113	15	-14.863587221601925
113	16	-13.466139001521352
113	17	-14.403726689160514
113	18	-13.536733824182352
113	19	-11.781591787297666
113	20	-12.868582292707295
113	21	-10.330148630780986
113	22	-10.1192032387547
113	23	-10.666419267823688
113	24	-10.32074952824953
113	25	-9.461407718915066
113	26	-8.335631908940732
113	27	-8.093737544318426
113	28	-7.74513719776955
113	29	-6.991584293973997
113	30	-6.928499753514462
113	31	-7.249369503862148
113	32	-6.697240204240206
113	33	-6.900960980196276
113	34	-6.490167642814699
113	35	-6.018459968952618
113	36	-6.757585418666304
113	37	-6.672773700156053
113	38	-6.556109143307674
113	39	-5.994855348899469
113	40	-5.8984146833558615
114	1	5.611584977114397
114	2	14.861441659647543
114	3	12.89159888559153
114	4	-15.876597781303659
114	5	-16.966823499213202
114	6	-15.43570217037864
114	7	-18.56245567667627
114	8	-17.42594040028599
114	9	-18.634544128257367
114	10	-12.439559340169637
114	11	-16.11452864619041
114	12	-16.390670098692155
114	13	-17.016883152794918
114	14	-17.286000242404658
114	15	-14.863587221601925
114	16	-13.466139001521352
114	17	-14.403726689160514
114	18	-13.536733824182352
114	19	-11.781591787297666
114	20	-12.868582292707295
114	21	-10.330148630780986
114	22	-10.1192032387547
114	23	-10.666419267823688
114	24	-10.32074952824953
114	25	-9.461407718915066
114	26	-8.335631908940732
114	27	-8.093737544318426
114	28	-7.74513719776955
114	29	-6.991584293973997
114	30	-6.928499753514462
114	31	-7.249369503862148
114	32	-6.697240204240206
114	33	-6.900960980196276
114	34	-6.490167642814699
114	35	-6.018459968952618
114	36	-6.757585418666304
114	37	-6.672773700156053
114	38	-6.556109143307674
114	39	-5.994855348899469
114	40	-5.8984146833558615
118	1	3.111584977114397
118	2	-0.13855834035245707
118	3	-2.108401114408469
118	4	6.1234022186963415
118	5	-8.966823499213202
118	6	-10.43570217037864
118	7	-12.062455676676269
118	8	-3.9259404002859917
118	9	-10.134544128257367
118	10	-10.939559340169637
118	11	-8.114528646190411
118	12	-0.39067009869215497
118	13	-2.516883152794918
118	14	-2.7860002424046577
118	15	-4.363587221601925
118	16	-10.966139001521352
118	17	-10.403726689160514
118	18	-2.5367338241823525
118	19	-8.781591787297666
118	20	-3.868582292707295
118	21	-3.330148630780986
118	22	2.3807967612453
118	23	-5.166419267823688
118	24	-3.3207495282495305
118	25	-7.961407718915066
118	26	-5.335631908940732
118	27	2.906262455681574
118	28	0.2548628022304502
118	29	-1.9915842939739967
118	30	6.071500246485538
118	31	8.250630496137852
118	32	8.802759795759794
118	33	8.599039019803724
118	34	9.009832357185301
118	35	0.4815400310473823
118	36	8.742414581333696
118	37	8.827226299843947
118	38	8.943890856692326
118	39	9.505144651100531
118	40	5.6015853166441385
119	1	9.111584977114397
119	2	17.361441659647543
119	3	12.39159888559153
119	4	16.62340221869634
119	5	13.533176500786798
119	6	15.06429782962136
119	7	6.937544323323731
119	8	6.074059599714008
119	9	10.365455871742633
119	10	2.0604406598303626
119	11	-3.6145286461904114
119	12	-7.390670098692155
119	13	-5.516883152794918
119	14	-6.286000242404658
119	15	-4.863587221601925
119	16	0.533860998478648
119	17	-8.903726689160514
119	18	-0.5367338241823525
119	19	-11.781591787297666
119	20	-12.868582292707295
119	21	-10.330148630780986
119	22	-10.1192032387547
119	23	-10.666419267823688
119	24	-10.32074952824953
119	25	-9.461407718915066
119	26	-8.335631908940732
119	27	-8.093737544318426
119	28	-7.74513719776955
119	29	-6.991584293973997
119	30	-6.928499753514462
119	31	-7.249369503862148
119	32	-6.697240204240206
119	33	-6.900960980196276
119	34	-6.490167642814699
119	35	-6.018459968952618
119	36	-6.757585418666304
119	37	-6.672773700156053
119	38	-6.556109143307674
119	39	-5.994855348899469
119	40	-5.8984146833558615
120	1	9.111584977114397
120	2	13.361441659647543
120	3	15.89159888559153
120	4	15.873402218696341
120	5	15.533176500786798
120	6	14.31429782962136
120	7	0.6875443233237313
120	8	7.074059599714008
120	9	2.8654558717426326
120	10	-4.689559340169637
120	11	-5.864528646190411
120	12	-3.890670098692155
120	13	-11.516883152794918
120	14	-9.786000242404658
120	15	-7.363587221601925
120	16	-13.466139001521352
120	17	-14.403726689160514
120	18	-13.536733824182352
120	19	-11.781591787297666
120	20	-12.868582292707295
120	21	-10.330148630780986
120	22	-10.1192032387547
120	23	-10.666419267823688
120	24	-10.32074952824953
120	25	-9.461407718915066
120	26	-8.335631908940732
120	27	-8.093737544318426
120	28	-7.74513719776955
120	29	-6.991584293973997
120	30	-6.928499753514462
120	31	-7.249369503862148
120	32	-6.697240204240206
120	33	-6.900960980196276
120	34	-6.490167642814699
120	35	-6.018459968952618
120	36	-6.757585418666304
120	37	-6.672773700156053
120	38	-6.556109143307674
120	39	-5.994855348899469
120	40	-5.8984146833558615
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1227.0
5	9.0
6	24.0
7	45.0
8	36.0
9	84.0
10	116.0
11	145.0
12	175.0
13	220.0
14	216.0
15	187.0
16	203.0
17	197.0
18	215.0
19	201.0
20	215.0
21	231.0
22	220.0
23	269.0
24	257.0
25	271.0
26	264.0
27	228.0
28	211.0
29	160.0
30	123.0
31	90.0
32	57.0
33	35.0
34	23.0
35	4.0
36	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	98.45352160026896	0.6219532694570516	0.5883341738107245	0.33619095646327113
8	97.44838005707571	0.8897095853617593	0.7218398522746349	0.9400705052878966
9	97.48195400369313	1.2758099714621454	0.7722007722007722	0.4700352526439483
10	32.611977856064414	41.16758933064922	11.038416373091762	15.182016440194598
11	29.256836101325277	28.183190739808754	26.33786277470223	16.22211038416373
12	40.37913101828553	17.78225130011743	20.14762623720852	21.690991444388523
13	26.623049823855062	22.747861097131352	25.012581781580273	25.616507297433316
14	26.740479785270928	24.27445059553766	26.67337695017615	22.311692669015265
15	26.757255494044625	27.42828384499245	24.77772185874853	21.036738802214394
16	28.21674215735615	20.181177654755913	27.679919476597885	23.922160711290054
17	30.58211709444724	22.79818822345244	22.580104009394397	24.03959067270592
18	29.726555946988757	25.53262875356484	21.355477268914612	23.38533803053179
19	19.639851901716593	21.507909794681925	17.283742847526085	41.5684954560754
20	24.39188055695353	24.173796342895486	22.982721019963094	28.45160208018789
21	22.34524408656266	21.456131521556788	30.716322764636807	25.482301627243753
22	20.265056198624393	20.58379466532461	28.58580775037745	30.565341385673545
23	24.86160040261701	24.24089917799027	28.736789129340718	22.160711290052003
24	23.905385002516354	24.324777721858748	26.80758262036571	24.962254655259184
25	23.651940198219386	26.17167814547287	25.835713085839075	24.340668570468672
26	20.503293362607668	19.38861678770478	34.18341496368857	25.92467488599899
27	21.825197114578092	17.144774366716995	33.232679080691156	27.797349438013757
28	15.338143983889916	23.5106561503608	35.072998825306264	26.078201040443027
29	17.946563602755838	20.55116787094606	35.97714669803395	25.525121828264158
30	16.45654731887712	14.641116153975458	43.990586653219026	24.91174987392839
31	16.4535666218035	17.227456258411845	46.23149394347241	20.087483176312247
32	22.95302013422819	13.37248322147651	42.030201342281885	21.644295302013422
33	16.92953020134228	18.42281879194631	44.73154362416108	19.916107382550337
34	13.621875524240897	15.920147626237208	48.6327797349438	21.825197114578092
35	16.68065111595905	17.133747273032387	47.35693908373888	18.828662527269678
36	15.771812080536913	19.58053691275168	46.577181208053695	18.07046979865772
37	16.238455079764904	18.28715365239295	46.49874055415617	18.97565071368598
38	13.741610738255034	19.42953020134228	48.053691275167786	18.7751677852349
39	14.750797113609664	20.439671085752643	44.65514348044974	20.15438832018795
40	12.732762959235028	19.979869149471565	44.489179667840965	22.79818822345244
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	2.0
14	3.5
15	5.0
16	12.0
17	19.0
18	19.0
19	34.0
20	49.0
21	79.0
22	109.0
23	109.0
24	121.0
25	133.0
26	137.5
27	142.0
28	142.0
29	165.0
30	188.0
31	218.0
32	248.0
33	248.0
34	284.5
35	321.0
36	348.5
37	376.0
38	376.0
39	380.0
40	384.0
41	415.5
42	447.0
43	447.0
44	426.5
45	406.0
46	376.5
47	347.0
48	347.0
49	355.5
50	364.0
51	353.0
52	342.0
53	342.0
54	348.5
55	355.0
56	388.5
57	422.0
58	422.0
59	415.5
60	409.0
61	350.5
62	292.0
63	292.0
64	270.0
65	248.0
66	204.0
67	160.0
68	160.0
69	126.5
70	93.0
71	76.0
72	59.0
73	59.0
74	40.5
75	22.0
76	16.5
77	11.0
78	11.0
79	6.5
80	2.0
81	2.5
82	3.0
83	3.0
84	2.0
85	1.0
86	1.5
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.20130850528434827
8	0.06710283509478275
9	0.06710283509478275
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.3187384667002181
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.1342056701895655
26	0.6710283509478275
27	0.0
28	0.033551417547391375
29	0.16775708773695688
30	0.20130850528434827
31	0.2851870491528267
32	0.016775708773695688
33	0.016775708773695688
34	0.0
35	0.033551417547391375
36	0.016775708773695688
37	0.10065425264217413
38	0.016775708773695688
39	0.033551417547391375
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	5961.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.45093105183693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.08056614044638	88.82737795671866
2	2.558519324986391	4.730749874182185
3	0.8528397749954637	2.3653749370910924
4	0.10887316276537834	0.40261701056869653
5	0.018145527127563055	0.08387854386847844
6	0.03629105425512611	0.20130850528434827
7	0.03629105425512611	0.23485992283173965
8	0.07258210851025222	0.536822680758262
9	0.018145527127563055	0.1509813789632612
>10	0.21774632553075668	2.4660291897332662
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	21	0.35228988424760943	No Hit
TAGAGAGGGATGAGATGGACTTAAATGAATTGACGGGTAC	15	0.25163563160543534	No Hit
TAGAGAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC	15	0.25163563160543534	No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	12	0.20130850528434827	No Hit
TAGAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	11	0.18453279651065257	No Hit
TAGAGAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC	11	0.18453279651065257	No Hit
TAGAGAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	11	0.18453279651065257	No Hit
TAGAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	11	0.18453279651065257	No Hit
TAGAGAGGGATGAGATGGACTCAAATGAATTGACGGGTAC	10	0.16775708773695688	No Hit
TAGAGAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC	10	0.16775708773695688	No Hit
TAGAGAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	10	0.16775708773695688	No Hit
TAGAGAGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC	10	0.16775708773695688	No Hit
TAGAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.1509813789632612	No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	8	0.1342056701895655	No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	8	0.1342056701895655	No Hit
TAGAGAGGGCAGAGTCGATTTTTTTTTTTTTTTTTTTTTT	8	0.1342056701895655	No Hit
TAGAGAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC	8	0.1342056701895655	No Hit
TAGAGAGGGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTT	7	0.11742996141586982	No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	7	0.11742996141586982	No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG	6	0.10065425264217413	No Hit
TAGAGAGGGCTCGTCTTACTCAGCAACATCTTTTGATACA	6	0.10065425264217413	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.016775708773695688	0.0	0.0	0.0	0.0
11	0.016775708773695688	0.0	0.0	0.0	0.0
12	0.016775708773695688	0.0	0.0	0.0	0.0
13	0.016775708773695688	0.0	0.0	0.0	0.0
14	0.016775708773695688	0.0	0.0	0.0	0.0
15	0.016775708773695688	0.0	0.0	0.0	0.0
16	0.016775708773695688	0.0	0.0	0.0	0.0
17	0.016775708773695688	0.0	0.0	0.0	0.0
18	0.016775708773695688	0.0	0.0	0.0	0.0
19	0.016775708773695688	0.0	0.0	0.0	0.0
20	0.016775708773695688	0.0	0.0	0.0	0.0
21	0.016775708773695688	0.0	0.0	0.0	0.0
22	0.016775708773695688	0.0	0.0	0.0	0.0
23	0.016775708773695688	0.0	0.0	0.0	0.0
24	0.016775708773695688	0.0	0.0	0.0	0.0
25	0.016775708773695688	0.0	0.0	0.0	0.0
26	0.016775708773695688	0.0	0.0	0.0	0.0
27	0.016775708773695688	0.0	0.0	0.0	0.0
28	0.016775708773695688	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGCT	20	0.0025729346	34.000004	6
GAAGCAG	20	0.0025729346	34.000004	9
AGGGCAG	20	0.0025729346	34.000004	6
GAGGGTG	20	0.0025729346	34.000004	5
GAGGGCA	40	6.019036E-8	34.000004	5
GGGGAGT	20	0.0025729346	34.000004	7
AGGGGGG	55	2.0008883E-11	34.0	6
AGAGGGT	55	2.0008883E-11	34.0	4
AGGGATA	30	1.251807E-5	34.0	6
AGGGACA	25	1.7967274E-4	34.0	6
AGGGAAG	50	2.8376235E-10	34.0	6
GGAAGCA	25	1.7967274E-4	34.0	8
GAGGGGG	85	0.0	34.0	5
GAGGGGC	30	1.251807E-5	34.0	5
GAGGGCC	25	1.7967274E-4	34.0	5
GAGGGAT	105	0.0	34.0	5
GAGGGAC	45	4.147296E-9	34.0	5
GAGGGAA	60	1.8189894E-12	34.0	5
TAGAGAG	590	0.0	33.42373	1
AGAGAGG	580	0.0	33.41379	2
>>END_MODULE