FastQCFastQC Report
Fri 17 Jun 2016
SRR1525903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3486
Sequences flagged as poor quality0
Sequence length40
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT792.266207687894435No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT712.0367183017785426No Hit
TAGAGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG501.4343086632243258No Hit
TAGAGAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT190.5450372920252439No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC170.4876649454962708No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC150.4302925989672977No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG110.3155479059093517No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG100.2868617326448652No Hit
TAGAGAGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT90.25817555938037867No Hit
TAGAGAGGGGGGGAGGTAGTGACGAAAAATAACAATACAG70.2008032128514056No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.17211703958691912No Hit
TAGAGAGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG60.17211703958691912No Hit
TAGAGAGGGTACCTGGTTGACCCTGCCAGTAGCATATGCT50.1434308663224326No Hit
TAGAGAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG50.1434308663224326No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1434308663224326No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG40.11474469305794606No Hit
TAGAGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11474469305794606No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGA40.11474469305794606No Hit
TAGAGAGGGTACATGGTTGATCCTGCCAGTAGCATATGCT40.11474469305794606No Hit
TAGAGAGGGGTGCAGTGCTTAGAGAGGGTCACAGATCCTC40.11474469305794606No Hit
TAGAGAGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA40.11474469305794606No Hit
TAGAGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGC40.11474469305794606No Hit
TAGAGAGGGACTAATCCTAGCCCTAGCCCTACACAAATAT40.11474469305794606No Hit
TAGAGAGGGATTTCTGGTCTGTTGACATGTAGCTAGCTAG40.11474469305794606No Hit
TAGAGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA40.11474469305794606No Hit
TAGAGAGGGGAGTCGAATTTTTTTTTTTTTTTTTTTTTTT40.11474469305794606No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGA40.11474469305794606No Hit
TAGAGAGGGTCACAGATACTCTTCTGAAGGAGATTGGGCG40.11474469305794606No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTTT251.640743E-434.00000431
GATGAGT251.640743E-434.00000428
AGATGAG251.640743E-434.00000427
GAGGGTA251.640743E-434.0000045
GAGGGGG1000.034.0000045
GAGGGAA251.640743E-434.0000045
AGTTTTT251.640743E-434.00000432
TGAGTTT251.640743E-434.00000430
ATGAGTT251.640743E-434.00000429
AGGGGGG700.034.06
CTGAGAT301.1012966E-534.024
AGAGGGT700.034.04
AGAGGGG1400.034.04
AGAGGGA750.034.04
TTTTTGT200.002422808434.034
GTTTTTG200.002422808434.033
AGGGTAC200.002422808434.06
TCAAAGA200.002422808434.010
GGGGGGG200.002422808434.07
TCTGAGA301.1012966E-534.023
Produced by FastQC (version 0.11.5)