FastQCFastQC Report
Fri 17 Jun 2016
SRR1525902_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525902_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14841
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT7324.9322821912270065No Hit
CCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC6354.278687419985176No Hit
CCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC2331.5699750690654268No Hit
CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1761.185903914830537No Hit
CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA1300.8759517552725558No Hit
CCAGACGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC1110.7479280371942592No Hit
CCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC870.586213866990095No Hit
CCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC580.39080924466006334No Hit
CCAGACGGGGCTCCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAA570.3840711542348898No Hit
CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT550.3705949733845428No Hit
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG550.3705949733845428No Hit
CCAGACGGGGCTCCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATT480.32342834040832824No Hit
CCAGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.31669024998315476No Hit
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT430.2897378882824608No Hit
CCAGACGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC390.26278552658176674No Hit
CCAGACGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA380.2560474361565932No Hit
CCAGACGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC350.2358331648810727No Hit
CCAGACGGGCTCCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTG310.2088808031803787No Hit
CCAGACGGGTCCCCCCTCCTCTTCTGAGATGAGTTTTTGTTCCCTCCGCCCAATCT310.2088808031803787No Hit
CCAGACGGGGCTCCAGACGGGTCACAGATCCTCTTCTGAGCTGAGTTTTTGTTCAA290.19540462233003167No Hit
CCAGACGGGCATCAGCGTAAACCGGGCTTCTCCTCCCAAAAAAAAAAAAAAAAAAA290.19540462233003167No Hit
CCAGACGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGCTTGAACAAAAACTCA290.19540462233003167No Hit
CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAAAAA280.18866653190485816No Hit
CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCTAATCCGCCCAATCT250.16845226062933766No Hit
CCAGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA230.15497607977899064No Hit
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTCTTTTTTTTTTTTTTT230.15497607977899064No Hit
CCAGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA220.14823798935381713No Hit
CCAGACGGGGCTCCAGACGGGTCACAGATCCTCTTCTGAACGAGATTGGGCGGATT190.1280237180782966No Hit
CCAGACGGGCATCAGCGTAAACCGGGCTTCTCCTCCCAAACAAAAAAAAAAAAAAA190.1280237180782966No Hit
CCAGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1212856276531231No Hit
CCAGACGGGCTCCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAAT180.1212856276531231No Hit
CCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAAACT180.1212856276531231No Hit
CCAGACGGCTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT180.1212856276531231No Hit
CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCTGAAGGAGAT170.11454753722794961No Hit
CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCCATCCGCCCAATCT170.11454753722794961No Hit
CCAGACGGGTCCCCGCTCCTCTTCTGAAGGAGATTGGGCGGACTGCCCAAAAACTC160.1078094468027761No Hit
CCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTA150.1010713563776026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCC309.092091E-750.5292141
GTTCCCT150.00792879150.5292139
GTTCCAT150.00792879150.5292139
TGTTCCC150.00792879150.5292138
TGTTCCA150.00792879150.5292138
TTCCCTC150.00792879150.5292140
AACCAAC150.00792879150.5292141
TTCCATC150.00792879150.5292140
TCCATCC150.00792879150.5292141
TCACTCC150.00792879150.5292141
GCCGATT251.8666977E-550.5292138
CCGATTG251.8666977E-550.5292139
TCCCTCC150.00792879150.5292141
CGATTGA309.092091E-750.5292140
TTTTGAT203.909241E-450.35616735
TTGTTCC354.561298E-850.35616737
CCAACAC203.909241E-450.35616743
TTCGCCC150.00803760450.35616745
AATTCGC150.00803760450.35616743
CATCCGC150.00803760450.35616743