##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525902_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14841 Sequences flagged as poor quality 0 Sequence length 56 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.34997641668351 39.0 38.0 39.0 33.0 40.0 2 36.82723536149855 39.0 38.0 39.0 32.0 40.0 3 36.15598679334277 38.0 36.0 39.0 30.0 40.0 4 35.65891786267772 38.0 35.0 39.0 28.0 40.0 5 35.40004042854255 38.0 36.0 39.0 26.0 40.0 6 35.64503739640186 38.0 35.0 39.0 28.0 40.0 7 36.141836803449905 38.0 36.0 39.0 31.0 40.0 8 36.196347954989555 38.0 36.0 39.0 31.0 40.0 9 36.15571726972576 38.0 36.0 39.0 30.0 40.0 10 35.020685937605286 38.0 35.0 39.0 27.0 40.0 11 34.67023785459201 38.0 34.0 39.0 26.0 40.0 12 33.825550838892255 38.0 33.0 39.0 22.0 40.0 13 32.674482851559866 36.0 31.0 39.0 18.0 40.0 14 33.98369382117108 38.0 33.0 39.0 22.0 40.0 15 31.312108348494036 35.0 28.0 38.0 14.0 40.0 16 32.05451115153966 36.0 29.0 38.0 18.0 40.0 17 30.767603261235767 35.0 26.0 38.0 14.0 40.0 18 30.990768816117512 35.0 26.0 38.0 15.0 40.0 19 32.003166902499835 36.0 28.0 39.0 18.0 40.0 20 32.507715113536825 36.0 29.0 39.0 20.0 40.0 21 33.69483188464389 38.0 33.0 39.0 20.0 40.0 22 32.87992722862341 37.0 31.0 39.0 17.0 40.0 23 33.897311501920356 38.0 33.0 39.0 21.0 40.0 24 33.046358062125194 37.0 31.0 39.0 18.0 40.0 25 33.4426925409339 38.0 32.0 39.0 20.0 40.0 26 33.09931945286706 37.0 32.0 39.0 19.0 40.0 27 33.47031871167711 38.0 33.0 39.0 21.0 40.0 28 33.595647193585336 38.0 33.0 39.0 22.0 40.0 29 32.90937268378141 37.0 32.0 39.0 18.0 40.0 30 32.88403746378276 37.0 31.0 39.0 18.0 40.0 31 33.35206522471532 37.0 33.0 39.0 20.0 40.0 32 33.370729735193045 38.0 33.0 39.0 20.0 40.0 33 32.91974934303618 37.0 32.0 39.0 18.0 40.0 34 32.54335961188599 36.0 32.0 39.0 17.0 40.0 35 33.242975540731756 38.0 33.0 39.0 18.0 40.0 36 31.99966309547874 36.0 31.0 38.0 10.0 40.0 37 31.85358129506098 36.0 31.0 38.0 4.0 40.0 38 31.241358399029714 35.0 30.0 38.0 4.0 40.0 39 29.613233609595042 35.0 27.0 38.0 4.0 39.0 40 27.225860791051815 33.0 17.0 38.0 4.0 39.0 41 24.39498686072367 29.0 10.0 36.0 4.0 39.0 42 25.357792601576712 30.0 16.0 35.0 4.0 39.0 43 26.52920962199313 32.0 18.0 37.0 4.0 39.0 44 26.077824944410754 31.0 15.0 37.0 4.0 39.0 45 26.43157469173236 32.0 18.0 37.0 4.0 39.0 46 24.449093726837813 29.0 12.0 35.0 4.0 39.0 47 23.911596253621724 28.0 8.0 35.0 4.0 39.0 48 23.991307863351526 28.0 9.0 35.0 4.0 39.0 49 23.434876356040697 28.0 5.0 35.0 4.0 39.0 50 24.16238797924668 29.0 4.0 35.0 4.0 39.0 51 24.369584259820765 30.0 4.0 35.0 4.0 39.0 52 24.289198841048446 30.0 4.0 35.0 4.0 39.0 53 24.2990364530692 30.0 4.0 35.0 4.0 39.0 54 23.787413247085777 30.0 4.0 36.0 4.0 39.0 55 24.32470857758911 31.0 4.0 36.0 4.0 39.0 56 22.733441142780137 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.2695601097174247 1 2 3.0212874398634852 1 3 3.565905589551491 1 4 4.069515865090359 1 5 4.648584338190766 1 6 3.858727092652785 1 7 2.9624697540806295 1 8 3.319253386333891 1 9 3.216427470856445 1 10 4.893391626526515 1 11 0.28723534725509126 1 12 -8.10893705797557 1 13 -16.864052026433598 1 14 -8.206551527133563 1 15 0.36929556993430523 1 16 -3.869281371993452 1 17 -4.634069171669413 1 18 -6.7977231672624505 1 19 -0.28990475115844205 1 20 -1.3767046475483014 1 21 5.3101462487890565 1 22 6.555842627652414 1 23 5.395917029958628 1 24 4.9876000820358435 1 25 5.313165713937192 1 26 6.521346681920026 1 27 5.266425330646307 1 28 5.452425775906821 1 29 6.608744310714506 1 30 5.934620863169627 1 31 4.314775473759408 1 32 4.351608486514067 1 33 5.331670832378268 1 34 3.9857981493337498 1 35 5.352936739501761 1 36 2.409183928794807 1 37 1.3533703387542424 1 38 2.1426229897696523 1 39 -0.7442153752823195 1 40 -14.801066283490051 1 41 -2.9950094843165616 1 42 -6.676792280489259 1 43 -9.815392713445542 1 44 -15.858048692936457 1 45 -5.671182501007195 1 46 -8.818704267367199 1 47 -14.749008837889836 1 48 -8.871404637101216 1 49 -14.327631645415696 1 50 -8.212559208722137 1 51 -5.293577598077022 1 52 -5.065192981861909 1 53 -5.200045783185683 1 54 -5.312059990777485 1 55 -5.416429680806807 1 56 -1.4069588943048679 2 1 2.799863140020456 2 2 3.472045015621056 2 3 4.418178316824218 2 4 4.342243137817633 2 5 5.311463126069555 2 6 4.858727092652785 2 7 3.678378844989723 2 8 2.667738234818735 2 9 3.7012759557049293 2 10 5.037331020465906 2 11 4.45011413513388 2 12 0.4440932450547308 2 13 2.47306918568761 2 14 5.17223635165432 2 15 5.543537994176731 2 16 2.8390519613398837 2 17 6.7182035556033135 2 18 3.539398044858764 2 19 7.119186157932468 2 20 5.21041656457291 2 21 6.0601462487890565 2 22 7.112660809470594 2 23 5.930007939049535 2 24 7.006539475975234 2 25 4.718468744240223 2 26 4.184225469798815 2 27 4.270213209434189 2 28 4.641819715300759 2 29 4.650410977381171 2 30 3.6012875298362914 2 31 1.5912906252745636 2 32 2.8667600016655825 2 33 0.8430344687419016 2 34 2.2357981493337498 2 35 3.394603406168425 2 36 4.026608171219049 2 37 3.482158217542125 2 38 4.229744201890863 2 39 5.1307846247176805 2 40 3.770903413479644 2 41 7.8042329399258605 2 42 5.323207719510741 2 43 5.014152741099913 2 44 -5.608048692936457 2 45 4.8023023474776565 2 46 3.9199320962691644 2 47 1.3229608590798598 2 48 4.571777181080602 2 49 -2.9071770999611495 2 50 4.8935013973384685 2 51 8.501876947377525 2 52 7.02950398783506 2 53 7.693893610753712 2 54 7.134909706192211 2 55 6.939630925253798 2 56 10.2597077723618 3 1 2.8331964733537944 3 2 3.6703783489543937 3 3 4.373178316824216 3 4 4.682243137817636 3 5 5.283129792736219 3 6 4.538727092652785 3 7 3.9733788449897247 3 8 4.121071568152068 3 9 3.754609289038264 3 10 5.260664353799243 3 11 5.0017808018005425 3 12 4.827426578388064 3 13 5.941402519020944 3 14 5.478903018320985 3 15 -0.0597953391566044 3 16 6.530718628006547 3 17 9.293203555603316 3 18 8.307731378192099 3 19 6.219186157932469 3 20 6.388749897906244 3 21 7.090146248789058 3 22 8.297660809470596 3 23 6.585007939049536 3 24 7.014872809308567 3 25 6.1568020775735555 3 26 6.175892136465482 3 27 5.47187987610085 3 28 5.865153048634095 3 29 6.348744310714508 3 30 7.054620863169625 3 31 4.852957291941227 3 32 5.393426668332246 3 33 5.268034468741899 3 34 5.725798149333752 3 35 6.232936739501763 3 36 8.598274837885715 3 37 8.278824884208792 3 38 8.068077535224202 3 39 9.11578462471768 3 40 11.162570080146313 3 41 13.775899606592528 3 42 12.283207719510742 3 43 11.57915274109991 3 44 12.421951307063544 3 45 11.850635680810992 3 46 12.604932096269167 3 47 14.094627525746525 3 48 13.406777181080603 3 49 13.657822900038848 3 50 13.480168064005138 3 51 12.051876947377522 3 52 11.871170654501725 3 53 12.387226944087043 3 54 11.991576372858876 3 55 11.736297591920469 3 56 11.85304110569513 4 1 2.5531964733537933 4 2 3.6303783489543946 4 3 4.093178316824215 4 4 3.96224313781763 4 5 4.7631297927362155 4 6 4.498727092652786 4 7 2.9333788449897256 4 8 2.36107156815207 4 9 3.6746092890382656 4 10 3.420664353799239 4 11 5.361780801800542 4 12 4.7874265783880645 4 13 6.101402519020947 4 14 5.478903018320985 4 15 6.620204660843392 4 16 6.490718628006547 4 17 9.773203555603313 4 18 8.627731378192099 4 19 8.059186157932466 4 20 7.748749897906244 4 21 6.5701462487890545 4 22 7.6976608094705945 4 23 6.905007939049536 4 24 6.174872809308571 4 25 5.476802077573556 4 26 5.495892136465482 4 27 2.911879876100855 4 28 5.585153048634094 4 29 5.348744310714508 4 30 6.214620863169628 4 31 5.37295729194123 4 32 3.1534266683322443 4 33 4.708034468741904 4 34 5.525798149333749 4 35 3.39293673950176 4 36 4.198274837885716 4 37 4.67882488420879 4 38 5.068077535224202 4 39 7.43578462471768 4 40 -1.277429919853688 4 41 12.65589960659253 4 42 8.72320771951074 4 43 10.419152741099914 4 44 9.661951307063546 4 45 9.410635680810987 4 46 10.684932096269165 4 47 10.254627525746528 4 48 11.286777181080605 4 49 11.25782290003885 4 50 11.040168064005133 4 51 9.931876947377525 4 52 8.071170654501728 4 53 8.787226944087045 4 54 10.83157637285888 4 55 9.936297591920464 4 56 8.17304110569513 5 1 2.8831964733537916 5 2 3.3887116822877275 5 3 4.168178316824218 5 4 4.383909804484297 5 5 5.436463126069555 5 6 5.067060425986121 5 7 3.3867121783230587 5 8 3.6260715681520708 5 9 3.784609289038265 5 10 5.287331020465906 5 11 5.7417808018005445 5 12 5.944093245054731 5 13 6.764735852354281 5 14 6.213903018320984 5 15 8.62687132751006 5 16 6.964051961339884 5 17 9.259870222269978 5 18 7.831064711525432 5 19 7.035852824599132 5 20 6.543749897906245 5 21 5.601812915455721 5 22 4.320994142803929 5 23 5.596674605716199 5 24 5.548206142641906 5 25 4.301802077573559 5 26 4.434225469798815 5 27 2.7285465427675177 5 28 4.350153048634095 5 29 4.650410977381171 5 30 3.934620863169627 5 31 2.7162906252745636 5 32 1.9084266683322468 5 33 2.0513678020752373 5 34 3.152464816000414 5 35 3.6862700728350966 5 36 5.443274837885713 5 37 5.357158217542125 5 38 5.563077535224199 5 39 6.6307846247176805 5 40 10.520903413479648 5 41 12.470899606592528 5 42 10.656541052844076 5 43 10.555819407766577 5 44 11.308617973730207 5 45 9.593969014144324 5 46 9.628265429602497 5 47 10.07296085907986 5 48 8.780110514413934 5 49 11.21782290003885 5 50 8.893501397338468 5 51 9.085210280710857 5 52 8.737837321168396 5 53 8.318893610753712 5 54 8.676576372858879 5 55 8.397964258587134 5 56 8.843041105695132 6 1 2.924863140020456 6 2 3.722045015621056 6 3 4.501511650157553 6 4 4.675576471150968 6 5 5.436463126069555 6 6 5.025393759319449 6 7 3.3867121783230587 6 8 3.8344049014854065 6 9 3.909609289038265 6 10 5.578997687132578 6 11 5.533447468467209 6 12 5.819093245054731 6 13 7.056402519020946 6 14 6.713903018320984 6 15 7.585204660843395 6 16 8.172385294673212 6 17 9.759870222269978 6 18 10.706064711525432 6 19 9.452519491265804 6 20 8.002083231239581 6 21 7.4351462487890565 6 22 7.945994142803929 6 23 6.7633412723828705 6 24 6.631539475975234 6 25 6.051802077573559 6 26 5.559225469798815 6 27 4.520213209434189 6 28 6.18348638196743 6 29 6.108744310714506 6 30 6.101287529836291 6 31 5.9662906252745636 6 32 5.575093334998911 6 33 6.134701135408566 6 34 4.902464816000414 6 35 5.311270072835097 6 36 8.193274837885713 6 37 8.107158217542125 6 38 8.979744201890863 6 39 9.547451291384345 6 40 6.10423674681298 6 41 14.054232939925864 6 42 12.906541052844076 6 43 12.097486074433249 6 44 12.975284640396879 6 45 13.302302347477653 6 46 15.086598762935829 6 47 15.239627525746528 6 48 15.030110514413938 6 49 14.884489566705515 6 50 16.060168064005136 6 51 15.21021028071086 6 52 14.071170654501728 6 53 14.11056027742038 6 54 14.801576372858879 6 55 12.77296425858713 6 56 14.218041105695132 7 1 2.224105564262885 7 2 2.748560167136212 7 3 2.429541953187851 7 4 2.114970410544906 7 5 3.603129792736219 7 6 2.9496361835618785 7 7 0.6442879358988165 7 8 1.137435204515704 7 9 1.9891547435837182 7 10 3.802482535617422 7 11 3.7872353472550913 7 12 4.6637902147517 7 13 5.3632207008391255 7 14 4.2479939274118905 7 15 5.142022842661575 7 16 5.5398095370974545 7 17 7.547749010148767 7 18 7.156822287283003 7 19 6.755549794296101 7 20 5.4869317160880655 7 21 3.3556007942436032 7 22 5.010388082197867 7 23 3.6686443026859017 7 24 3.760327354763117 7 25 4.904074804846285 7 26 4.112255772829112 7 27 2.9936980579190333 7 28 3.0887894122704616 7 29 2.6996534016236 7 30 4.343711772260534 7 31 0.8147754737594077 7 32 1.7152448501504267 7 33 3.7862162869237217 7 34 1.440343603879203 7 35 2.2165731031381277 7 36 3.86372938334026 7 37 4.580643066026969 7 38 4.642622989769652 7 39 3.7103300792631337 7 40 6.2898428074190385 7 41 9.686808697501618 7 42 8.73229862860165 7 43 7.68460728655446 7 44 7.323769488881727 7 45 5.556090226265535 7 46 6.681295732632801 7 47 6.160082071201074 7 48 5.6285953628987855 7 49 7.763277445493397 7 50 7.0147135185505896 7 51 4.842786038286615 7 52 4.162079745410818 7 53 4.84540876226886 7 54 6.006121827404332 7 55 6.583570319193193 7 56 3.9566774693314954 8 1 2.6808155209728426 8 2 3.192283110859151 8 3 4.197940221586123 8 4 4.366052661627158 8 5 5.460272649879073 8 6 4.76348899741469 8 7 3.5295693211801975 8 8 3.620119187199691 8 9 2.844133098562075 8 10 5.1444738776087675 8 11 6.1941617541814935 8 12 6.9798075307690155 8 13 7.800450138068566 8 14 6.743664923082889 8 15 7.984014184652921 8 16 6.987861485149402 8 17 9.759870222269978 8 18 9.890588521049242 8 19 8.571567110313417 8 20 7.859226088382435 8 21 7.381574820217626 8 22 6.451946523756309 8 23 6.257388891430484 8 24 6.167253761689519 8 25 6.010135410906894 8 26 6.547320707894048 8 27 4.930927495148474 8 28 4.987057810538854 8 29 5.96588716785736 8 30 6.410811339360102 8 31 4.371052530036469 8 32 4.461998096903677 8 33 5.658510659218091 8 34 5.247702911238509 8 35 6.448174834739856 8 36 5.318274837885713 8 37 4.208348693732599 8 38 5.7118870590337245 8 39 7.374832243765301 8 40 -3.3898108722346407 8 41 10.048280558973481 8 42 7.799398195701215 8 43 8.234390836338008 8 44 7.713379878492116 8 45 8.010635680810989 8 46 8.902074953412022 8 47 11.567008478127477 8 48 10.018205752509175 8 49 12.360680042895996 8 50 10.703025206862282 8 51 11.013781709282284 8 52 9.261646844977918 8 53 11.36056027742038 8 54 7.980147801430306 8 55 9.380107115729992 8 56 8.854945867599895 9 1 2.014148854306171 9 2 2.4303783489543918 9 3 2.816987840633743 9 4 3.461290756865253 9 5 4.65074884035527 9 6 2.5253937593194493 9 7 2.4343312259421026 9 8 2.905833472913976 9 9 2.93937119380017 9 10 3.573045306180191 9 11 4.95606651608626 9 12 4.9798075307690155 9 13 6.562354899973325 9 14 5.696045875463838 9 15 7.1268713275100595 9 16 7.321194818482738 9 17 8.331298793698554 9 18 8.557255187715906 9 19 6.762043300789614 9 20 4.716368945525296 9 21 6.000622439265246 9 22 6.499565571375353 9 23 4.781198415240009 9 24 4.976777571213329 9 25 3.6291830299545076 9 26 3.975892136465479 9 27 2.0737846380056126 9 28 3.93943876291981 9 29 4.251601453571652 9 30 4.410811339360102 9 31 1.3710525300364687 9 32 1.4143790492846264 9 33 1.087082087789522 9 34 1.6762743398099396 9 35 1.9243653109303303 9 36 4.318274837885713 9 37 4.065491550875453 9 38 4.902363249509914 9 39 5.231975100908155 9 40 1.5149510325272644 9 41 9.524471035163955 9 42 8.70416010046312 9 43 8.710581312528483 9 44 7.332427497539733 9 45 6.629683299858609 9 46 8.806836858173927 9 47 8.662246573365575 9 48 9.399158133461555 9 49 10.360680042895993 9 50 9.13159663543371 9 51 8.061400756901335 9 52 7.975932559263633 9 53 8.789131705991807 9 54 6.980147801430306 9 55 8.808678544301419 9 56 7.902564915218942 10 1 2.9513782915356117 10 2 3.8394692580452983 10 3 4.565905589551491 10 4 4.796788592363086 10 5 5.557675247281672 10 6 4.813272547198238 10 7 3.553378844989723 10 8 4.137435204515704 10 9 4.034609289038265 10 10 5.166118899253789 10 11 5.605417165436911 10 12 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-1.6301458958424746 114 49 -8.705254023038073 114 50 -0.593678089841017 114 51 2.021107716608295 114 52 2.6865552698863446 114 53 1.7195346363947372 114 54 -0.9868851656026614 114 55 -0.06677933115645729 114 56 2.554579567233592 115 1 1.0178118579691784 115 2 1.8149937335697786 115 3 2.6777937014396045 115 4 2.534550830125326 115 5 3.9877451773516057 115 6 2.935650169575865 115 7 2.2841480757589565 115 8 2.2318407989213043 115 9 2.3423015967305716 115 10 3.697587430722322 115 11 0.7033192633390044 115 12 -4.58795803699655 115 13 1.7967871344055588 115 14 2.915826095244064 115 15 -5.6167184160796815 115 16 3.2845647818527013 115 17 7.0932035556033135 115 18 6.824654455115176 115 19 3.8499553887017015 115 20 5.2072114363677855 115 21 5.194761633404443 115 22 5.429968501778287 115 23 4.689623323664922 115 24 4.984103578539337 115 25 3.753725154496639 115 26 4.360507521080866 115 27 4.542649106870087 115 28 4.840537664018708 115 29 5.416436618406813 115 30 5.6269285554773205 115 31 4.825264984248921 115 32 4.1872728221784 115 33 4.083419084126515 115 34 5.255028918564516 115 35 4.814475201040221 115 36 3.4721209917318667 115 37 4.629594114978023 115 38 5.495769842916506 115 39 6.6788615477946 115 40 9.562570080146312 115 41 12.095899606592528 115 42 11.015515411818434 115 43 9.754537356484526 115 44 2.5265666916789264 115 45 8.472174142349449 115 46 4.660316711653781 115 47 -7.385372474253472 115 48 3.677546411849832 115 49 -0.16679248457653273 115 50 7.098629602466676 115 51 7.098030793531372 115 52 7.532709116040188 115 53 6.796457713317814 115 54 7.551576372858879 115 55 7.625528361151236 115 56 10.785348798002826 116 1 2.5776409177982345 116 2 3.3748227933988346 116 3 3.348733872379775 116 4 4.7311320267065184 116 5 5.658685348291776 116 6 3.5809493148750065 116 7 3.3311566227675016 116 8 3.723293790374292 116 9 3.7012759557049293 116 10 3.5095532426881277 116 11 4.797336357356102 116 12 3.6385376894991808 116 13 5.403624741243167 116 14 5.5611252405432055 116 15 -3.4842397836010477 116 16 5.964051961339884 116 17 8.370981333381092 116 18 7.4143980448587605 116 19 7.674741713488025 116 20 6.390972120128467 116 21 6.976812915455721 116 22 8.348771920581708 116 23 7.082785716827313 116 24 7.4370950315307915 116 25 7.176802077573559 116 26 6.142558803132143 116 27 5.311879876100853 116 28 5.725153048634095 116 29 6.442077644047842 116 30 7.045731974280741 116 31 5.244068403052342 116 32 4.97787111277669 116 33 4.190256690964123 116 34 5.708020371555971 116 35 6.075158961723982 116 36 7.207163726774599 116 37 8.39882488420879 116 38 7.743633090779756 116 39 9.533562402495459 116 40 12.173681191257426 116 41 9.595899606592528 116 42 6.4343188306218515 116 43 2.5280416299888024 116 44 2.253062418174654 116 45 5.066191236366546 116 46 2.4893765407136073 116 47 -4.496483585364583 116 48 1.7801105144139342 116 49 5.551156233372183 116 50 4.726834730671804 116 51 7.4740991695997465 116 52 7.293392876723949 116 53 7.360560277420376 116 54 8.607131928414436 116 55 5.745186480809355 116 56 8.593041105695132 117 1 1.0617679019252222 117 2 1.644664063240107 117 3 2.007464031109933 117 4 1.8422431378176327 117 5 3.817415507021934 117 6 3.358727092652785 117 7 1.910521702132577 117 8 1.4296429967235014 117 9 1.3203235747525497 117 10 2.9063786395135267 117 11 -2.7582191981994555 117 12 -7.9011448501833605 117 13 -0.675740338121912 117 14 -3.3039541245361583 117 15 -6.182652482013747 117 16 -8.940709943422025 117 17 2.807489269889029 117 18 1.033445663906381 117 19 0.4049004436467527 117 20 2.6687498979062454 117 21 4.524431963074772 117 22 4.094803666613448 117 23 3.662150796192389 117 24 5.072015666451424 117 25 3.6768020775735586 117 26 4.833034993608333 117 27 3.4547370189579993 117 28 2.296581620062664 117 29 4.823030025000222 117 30 2.6489065774553424 117 31 3.7758144347983738 117 32 2.747712382617962 117 33 3.182320183027617 117 34 2.4143695779051804 117 35 3.4957938823589068 117 36 5.103989123599998 117 37 4.041682027065935 117 38 3.545220392367053 117 39 2.3272131961462534 117 40 3.1339986515748848 117 41 0.16732817802110134 117 42 0.6089220052250255 117 43 -6.503704401757229 117 44 -7.786620121507884 117 45 -2.0607928906175843 117 46 -6.026496475159407 117 47 -7.671086759967759 117 48 -5.19607996177654 117 49 -8.996462814246863 117 50 -5.511260507423435 117 51 -4.03383733833676 117 52 -2.714543631212557 117 53 -2.901344484484383 117 54 -1.8769950557125483 117 55 -2.572273836650961 117 56 0.6644696771237051 118 1 1.4189107590680763 118 2 2.787521206097246 118 3 3.150321173967072 118 4 3.556528852103348 118 5 3.745986935593365 118 6 3.715869949795639 118 7 3.410521702132577 118 8 2.9296429967235014 118 9 3.4631807176096956 118 10 3.4063786395135267 118 11 3.027495087514829 118 12 2.5274265783880665 118 13 2.252831090449515 118 14 -1.9468112673933007 118 15 -4.182652482013747 118 16 -5.154995657707737 118 17 -1.0496535872538288 118 18 -2.395125764665046 118 19 -1.0236709849246743 118 20 -0.8312501020937546 118 21 5.024431963074772 118 22 4.737660809470594 118 23 3.5907222247638195 118 24 4.500587095022855 118 25 5.033944934716413 118 26 5.4044635650369095 118 27 5.097594161815138 118 28 5.582295905776949 118 29 5.394458596428791 118 30 4.577478006026773 118 31 4.132957291941228 118 32 3.9619980969036774 118 33 4.396605897313332 118 34 2.7715124350480345 118 35 4.352936739501761 118 36 5.461131980742859 118 37 5.898824884208789 118 38 5.473791820938484 118 39 8.898641767574826 118 40 8.991141508717742 118 41 9.453042463735386 118 42 6.823207719510741 118 43 4.7820098839570555 118 44 7.57052273563497 118 45 5.867778537953846 118 46 1.6877892391263067 118 47 3.9717703828893853 118 48 0.946777181080602 118 49 3.574965757181708 118 50 3.4887394925765634 118 51 9.609019804520383 118 52 9.785456368787443 118 53 8.74151265837276 118 54 10.408719230001736 118 55 9.784869020491893 118 56 11.02161253426656 119 1 0.6331964733537916 119 2 1.041489460065506 119 3 2.015400539046439 119 4 2.6200209155954113 119 5 3.4364631260695546 119 6 1.4142826482083422 119 7 1.7756010672119444 119 8 1.612182679263185 119 9 1.4790537334827079 119 10 3.065108798243685 119 11 -1.2582191981994555 119 12 -7.528128977167491 119 13 -1.429708592090165 119 14 -6.049985870567905 119 15 -7.706462005823273 119 16 -8.702614705326784 119 17 0.25987022226998135 119 18 -1.585601955141236 119 19 -0.6585916198453106 119 20 -1.2756945465381975 119 21 1.1990351376779422 119 22 2.2376608094705936 119 23 0.6383412723828705 119 24 1.2148728093085701 119 25 2.1212465220180015 119 26 2.642558803132143 119 27 1.9785465427675177 119 28 1.836264159745209 119 29 2.442077644047842 119 30 2.7123986409474057 119 31 2.4662906252745636 119 32 1.9223155572211326 119 33 2.4124789131863444 119 34 1.8746870382226355 119 35 1.1307145172795394 119 36 2.9293859489968277 119 37 4.176602661986568 119 38 4.132521979668642 119 39 5.978006846939902 119 40 7.562570080146312 119 41 6.81812182881475 119 42 3.9343188306218515 119 43 -1.4719583700111976 119 44 -0.9691598040475675 119 45 5.566191236366546 119 46 1.2671543184913858 119 47 -1.1631502520312509 119 48 -3.719889485586066 119 49 -1.6710659888500388 119 50 -0.10649860266153155 119 51 4.4740991695997465 119 52 3.2933928767239493 119 53 4.138338055198155 119 54 1.8293541506366573 119 55 4.0785198141426875 119 56 7.093041105695132 120 1 2.3979023557067336 120 2 3.1950842313073338 120 3 4.05788419917716 120 4 3.9598901966411617 120 5 5.191365086853864 120 6 4.27049179853514 120 7 3.465143550872078 120 8 3.412836274034426 120 9 3.740491641979439 120 10 5.267723177328655 120 11 1.8888396253299575 120 12 1.9686030489762985 120 13 1.4755201660797717 120 14 1.427138312438629 120 15 1.4013811314316307 120 16 -1.4575166661111005 120 17 1.6226153203091975 120 18 -0.31109215121966827 120 19 2.0309508638148195 120 20 2.3746322508474194 120 21 5.1042638958478825 120 22 7.03177845652942 120 23 4.834419703755419 120 24 4.450166926955628 120 25 6.088566783455914 120 26 5.917068607053711 120 27 5.017762229042027 120 28 4.401623636869392 120 29 5.049920781302738 120 30 5.816973804346098 120 31 5.89766317429417 120 32 5.915779609508718 120 33 4.820975645212489 120 34 3.662268737569047 120 35 3.588230857148819 120 36 5.553568955532771 120 37 5.398824884208789 120 38 6.129254005812431 120 39 7.2851963894235645 120 40 9.327275962499254 120 41 -8.25704156987806 120 42 -6.794439339312788 120 43 -6.096141376547145 120 44 -14.387460457642339 120 45 -2.812893730953718 120 46 -7.131538491966129 120 47 -4.62066659190053 120 48 -3.906163995389985 120 49 -14.78217709996115 120 50 -3.3221848771713347 120 51 3.4283475356128186 120 52 2.541758889795844 120 53 2.7331092970282214 120 54 4.022164608152995 120 55 2.973944650743995 120 56 4.622452870401016 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 274.0 5 34.0 6 43.0 7 51.0 8 63.0 9 75.0 10 35.0 11 51.0 12 29.0 13 35.0 14 40.0 15 70.0 16 77.0 17 91.0 18 128.0 19 147.0 20 221.0 21 236.0 22 300.0 23 348.0 24 407.0 25 480.0 26 496.0 27 539.0 28 475.0 29 546.0 30 593.0 31 688.0 32 799.0 33 898.0 34 977.0 35 1085.0 36 1168.0 37 1322.0 38 1071.0 39 947.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.2385957819554 0.5659995957145745 0.1347618085034701 0.06064281382656155 8 98.83431035644499 0.9298564786739438 0.1212856276531231 0.11454753722794961 9 92.6756957078364 1.2061181861060577 4.709925207196281 1.4082608988612628 10 19.89084293511219 15.383060440671114 51.411629944073844 13.314466680142848 11 9.70285021224985 25.523886530557238 7.944208611279563 56.829054645913345 12 18.617343844754398 50.077488039889495 12.000539047234014 19.304629068122097 13 7.964422882555083 14.891179839633448 7.614042180446061 69.5303550973654 14 12.31722929721717 62.805740853042245 6.825685600700761 18.051344249039822 15 59.40674522551809 19.050521468237843 8.120005417851822 13.422727888392252 16 15.0293304564763 54.365855303081375 19.546895017193716 11.0579192232486 17 16.711590296495956 15.943396226415093 52.77628032345013 14.568733153638814 18 16.232059834242975 9.17054106866114 11.643420254699818 62.95397884239606 19 15.05999730349198 11.979236888229742 19.677767291357693 53.282998516920586 20 14.550853749072012 19.11318080583114 52.91219545117095 13.423769993925896 21 12.943871706758307 12.371134020618557 21.002627855265818 53.68236641735732 22 8.651708105922781 12.937133616333133 58.84374368304023 19.567414594703862 23 11.232396738764235 19.284414796846576 50.252678390944006 19.23051007344519 24 9.931945286705748 24.580553871032947 14.062394717337106 51.42510612492419 25 16.7475400997439 15.54791750909826 50.48524059846341 17.219301792694434 26 60.64977082771637 21.575896468050686 8.526557023456457 9.247775680776488 27 19.484897518878103 57.96251348435815 8.02319309600863 14.529395900755123 28 34.93935309973046 46.15229110512129 10.970350404312669 7.93800539083558 29 37.17196249072388 37.01679821898401 17.304189435336976 8.507049854955136 30 31.474989888094917 21.275448294458677 33.83443440744236 13.415127410004043 31 36.56399541686325 35.69454741524567 16.18925658825908 11.552200579632 32 33.60517624856777 37.57498146525578 17.948372312462087 10.871469973714362 33 35.6651376146789 33.24338909875877 16.46654074473826 14.624932541824068 34 13.669501822600244 15.006075334143379 60.82084514648306 10.503577696773322 35 12.456980902894932 18.77994466563196 57.93913219515486 10.82394223631824 36 31.199838133135493 16.301342146084846 37.41822351116207 15.080596209617589 37 33.135415963835094 15.768166790365024 40.395384926793064 10.701032319006815 38 37.956647984333856 17.334053616044297 33.17577149030995 11.533526909311904 39 32.12043475325728 22.662526159454533 15.850941740363195 29.366097346924995 40 28.47439206629534 16.54666485531857 34.37033011819046 20.60861296019563 41 23.06173847934109 15.574161050983596 30.10006126199714 31.264039207678167 42 14.633157681394406 38.58262396973382 19.26766653154979 27.516551817321982 43 11.135149753228314 40.328578189439526 31.79636265296464 16.73990940436752 44 12.330715059588298 40.269501625135426 33.477789815817985 13.921993499458289 45 28.330295050975625 23.59732631152522 34.98075754506785 13.091621092431302 46 10.181867351767968 38.9087958893922 19.82962612399432 31.079710634845515 47 12.6685183930628 33.58851026353228 15.615473206422328 38.12749813698259 48 31.595629889398435 23.772592392770434 16.21257081197734 28.419206905853788 49 12.29552521292416 37.23806948763012 16.668919832364473 33.79748546708125 50 11.972449186305626 41.05611452495104 16.469714362887434 30.501721925855897 51 12.350436017035085 42.22943283985669 15.892651929966876 29.52747921314135 52 13.566074684313593 59.024917280032405 18.67783104868661 8.731176986967384 53 11.241803555735821 55.25586426012303 19.820185222740484 13.682146961400662 54 9.509969584319027 28.82054748225752 33.774923960797565 27.894558972625887 55 10.432724814063556 24.705882352941178 37.58620689655172 27.275185936443542 56 11.83108108108108 25.83783783783784 36.263513513513516 26.06756756756757 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 3.0 8 3.0 9 3.0 10 3.0 11 3.0 12 35.0 13 76.0 14 85.0 15 77.0 16 69.0 17 59.0 18 49.0 19 54.0 20 59.0 21 64.5 22 65.0 23 60.0 24 72.0 25 84.0 26 94.5 27 105.0 28 109.5 29 129.5 30 145.0 31 134.5 32 124.0 33 123.5 34 123.0 35 137.0 36 151.0 37 179.5 38 209.0 39 210.0 40 213.5 41 217.0 42 271.5 43 326.0 44 409.5 45 493.0 46 723.5 47 1628.0 48 2302.0 49 2531.0 50 2760.0 51 2105.0 52 1450.0 53 1136.0 54 809.5 55 797.0 56 762.5 57 728.0 58 714.5 59 701.0 60 602.5 61 504.0 62 422.0 63 292.5 64 245.0 65 198.0 66 151.0 67 126.0 68 101.0 69 93.5 70 86.0 71 65.0 72 32.0 73 20.0 74 27.5 75 35.0 76 25.0 77 15.0 78 13.0 79 6.5 80 2.0 81 2.5 82 3.0 83 2.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5053567818880129 16 0.06738090425173505 17 0.006738090425173506 18 0.0 19 0.04716663297621454 20 0.16171417020416412 21 0.0 22 0.0 23 0.0 24 0.0 25 0.020214271275520515 26 0.03369045212586753 27 0.06064281382656155 28 0.006738090425173506 29 0.1212856276531231 30 0.04716663297621454 31 0.026952361700694025 32 0.026952361700694025 33 0.11454753722794961 34 0.18192844147968465 35 0.14823798935381713 36 0.09433326595242908 37 0.1347618085034701 38 0.2156188936055522 39 0.18866653190485816 40 0.8018327605956471 41 1.0107135637760258 42 0.26278552658176674 43 0.3369045212586753 44 0.4918806010376659 45 0.2021427127552052 46 0.3369045212586753 47 0.5390472340138804 48 0.08759517552725558 49 0.31669024998315476 50 0.2156188936055522 51 0.32342834040832824 52 0.2156188936055522 53 0.32342834040832824 54 0.3099521595579813 55 0.3436426116838488 56 0.27626170743211376 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 14841.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.84461963479549 #Duplication Level Percentage of deduplicated Percentage of total 1 90.51371264838313 59.59840981065966 2 5.014326647564469 6.603328616670036 3 1.6270978305362258 3.214069132807762 4 0.7981989357347523 2.102284212654134 5 0.4809660253786328 1.5834512499157738 6 0.255832992222677 1.0107135637760258 7 0.2967662709783054 1.3678323563102217 8 0.19443307408923455 1.0241897446263728 9 0.12279983626688497 0.7277137659187386 >10 0.5832992222677036 7.074994946432181 >50 0.0511665984445354 2.102284212654134 >100 0.04093327875562833 4.379758776362778 >500 0.020466639377814164 9.210969611212183 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 732 4.9322821912270065 No Hit CCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 635 4.278687419985176 No Hit CCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 233 1.5699750690654268 No Hit CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 176 1.185903914830537 No Hit CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 130 0.8759517552725558 No Hit CCAGACGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC 111 0.7479280371942592 No Hit CCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC 87 0.586213866990095 No Hit CCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC 58 0.39080924466006334 No Hit CCAGACGGGGCTCCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAA 57 0.3840711542348898 No Hit CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT 55 0.3705949733845428 No Hit CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 55 0.3705949733845428 No Hit CCAGACGGGGCTCCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATT 48 0.32342834040832824 No Hit CCAGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.31669024998315476 No Hit CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 43 0.2897378882824608 No Hit CCAGACGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 39 0.26278552658176674 No Hit CCAGACGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA 38 0.2560474361565932 No Hit CCAGACGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC 35 0.2358331648810727 No Hit CCAGACGGGCTCCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTG 31 0.2088808031803787 No Hit CCAGACGGGTCCCCCCTCCTCTTCTGAGATGAGTTTTTGTTCCCTCCGCCCAATCT 31 0.2088808031803787 No Hit CCAGACGGGGCTCCAGACGGGTCACAGATCCTCTTCTGAGCTGAGTTTTTGTTCAA 29 0.19540462233003167 No Hit CCAGACGGGCATCAGCGTAAACCGGGCTTCTCCTCCCAAAAAAAAAAAAAAAAAAA 29 0.19540462233003167 No Hit CCAGACGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGCTTGAACAAAAACTCA 29 0.19540462233003167 No Hit CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAAAAA 28 0.18866653190485816 No Hit CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCTAATCCGCCCAATCT 25 0.16845226062933766 No Hit CCAGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 23 0.15497607977899064 No Hit CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTCTTTTTTTTTTTTTTT 23 0.15497607977899064 No Hit CCAGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 22 0.14823798935381713 No Hit CCAGACGGGGCTCCAGACGGGTCACAGATCCTCTTCTGAACGAGATTGGGCGGATT 19 0.1280237180782966 No Hit CCAGACGGGCATCAGCGTAAACCGGGCTTCTCCTCCCAAACAAAAAAAAAAAAAAA 19 0.1280237180782966 No Hit CCAGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.1212856276531231 No Hit CCAGACGGGCTCCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAAT 18 0.1212856276531231 No Hit CCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAAACT 18 0.1212856276531231 No Hit CCAGACGGCTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 18 0.1212856276531231 No Hit CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCTGAAGGAGAT 17 0.11454753722794961 No Hit CCAGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCCATCCGCCCAATCT 17 0.11454753722794961 No Hit CCAGACGGGTCCCCGCTCCTCTTCTGAAGGAGATTGGGCGGACTGCCCAAAAACTC 16 0.1078094468027761 No Hit CCAGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTA 15 0.1010713563776026 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.013476180850347012 0.0 0.0 0.0 0.0 11 0.026952361700694025 0.0 0.0 0.0 0.0 12 0.026952361700694025 0.0 0.0 0.0 0.0 13 0.03369045212586753 0.0 0.0 0.0 0.0 14 0.1078094468027761 0.0 0.0 0.0 0.0 15 0.1078094468027761 0.0 0.0 0.0 0.0 16 0.1078094468027761 0.0 0.0 0.0 0.0 17 0.1145475372279496 0.0 0.0 0.0 0.0 18 0.1145475372279496 0.0 0.0 0.0 0.0 19 0.1145475372279496 0.0 0.0 0.0 0.0 20 0.1212856276531231 0.0 0.0 0.0 0.0 21 0.1212856276531231 0.0 0.0 0.0 0.0 22 0.1280237180782966 0.0 0.0 0.0 0.0 23 0.1347618085034701 0.0 0.0 0.0 0.0 24 0.14149989892864362 0.0 0.0 0.0 0.0 25 0.14823798935381713 0.0 0.0 0.0 0.0 26 0.15497607977899064 0.0 0.0 0.0 0.0 27 0.16845226062933766 0.0 0.0 0.0 0.0 28 0.19540462233003167 0.0 0.0 0.0 0.0 29 0.19540462233003167 0.0 0.0 0.0 0.0 30 0.19540462233003167 0.0 0.0 0.0 0.0 31 0.22235698403072568 0.0 0.0 0.0 0.0 32 0.2290950744558992 0.0 0.0 0.0 0.0 33 0.2358331648810727 0.0 0.0 0.0 0.0 34 0.24930934573141972 0.0 0.0 0.0 0.0 35 0.2560474361565932 0.0 0.0 0.0 0.0 36 0.2560474361565932 0.0 0.0 0.0 0.0 37 0.2560474361565932 0.0 0.0 0.0 0.0 38 0.26278552658176674 0.0 0.0 0.0 0.0 39 0.26278552658176674 0.0 0.0 0.0 0.0 40 0.27626170743211376 0.0 0.0 0.0 0.0 41 0.28299979785728724 0.0 0.0 0.0 0.0 42 0.30321406913280774 0.0 0.0 0.0 0.0 43 0.31669024998315476 0.0 0.0 0.0 0.0 44 0.37059497338454284 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAATCC 30 9.092091E-7 50.52921 41 GTTCCCT 15 0.007928791 50.52921 39 GTTCCAT 15 0.007928791 50.52921 39 TGTTCCC 15 0.007928791 50.52921 38 TGTTCCA 15 0.007928791 50.52921 38 TTCCCTC 15 0.007928791 50.52921 40 AACCAAC 15 0.007928791 50.52921 41 TTCCATC 15 0.007928791 50.52921 40 TCCATCC 15 0.007928791 50.52921 41 TCACTCC 15 0.007928791 50.52921 41 GCCGATT 25 1.8666977E-5 50.52921 38 CCGATTG 25 1.8666977E-5 50.52921 39 TCCCTCC 15 0.007928791 50.52921 41 CGATTGA 30 9.092091E-7 50.52921 40 TTTTGAT 20 3.909241E-4 50.356167 35 TTGTTCC 35 4.561298E-8 50.356167 37 CCAACAC 20 3.909241E-4 50.356167 43 TTCGCCC 15 0.008037604 50.356167 45 AATTCGC 15 0.008037604 50.356167 43 CATCCGC 15 0.008037604 50.356167 43 >>END_MODULE