FastQCFastQC Report
Fri 17 Jun 2016
SRR1525901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4022
Sequences flagged as poor quality0
Sequence length40
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT140.34808552958727No Hit
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT90.22376926902038788No Hit
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC80.19890601690701143No Hit
CCAGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGG80.19890601690701143No Hit
CCAGACGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG70.174042764793635No Hit
CCAGACGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC70.174042764793635No Hit
CCAGACGGGTACGAGATTTTTTTTTTTTTTTTTTTTTTTT70.174042764793635No Hit
CCAGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT70.174042764793635No Hit
CCAGACGGGATCCTAATGTGCGCCCTGTACCTTTGGAACT50.12431626056688214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG551.6370905E-1133.8256
CGGGGAG251.7427708E-433.8256
GACGGGA1150.033.8254
CGGGACA200.002534663833.8256
ACGGGGT200.002534663833.8255
ACGGGGG850.033.8255
ACGGGGA301.1903176E-533.8255
ACGGGAC301.1903176E-533.8255
ACGGGAA551.6370905E-1133.8255
GGAAGCA200.002534663833.8258
GGGACAT200.002534663833.8257
GACGGGC453.616151E-933.8249974
CGGGAAG358.070201E-733.8249976
CCAGACG3950.033.3968351
CAGACGG3900.033.3913462
AGACGGG3750.033.3743
GACGGGG1600.032.7679674
GGGGGGG250.007558845427.0600017
ACGGGGC250.007558845427.0600015
GGGAAGC250.007558845427.0600017