FastQCFastQC Report
Fri 17 Jun 2016
SRR1525898_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525898_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138276
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG26281.9005467326217131No Hit
GAGGAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT7370.532991987040412No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG7030.5084034828892939No Hit
GAGGAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC6680.4830917874396135No Hit
GAGGAGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT6510.47079753536405455No Hit
GAGGAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC5640.4078798923891348No Hit
GAGGAGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC3590.2596256761838642No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG3400.24588504151118054No Hit
GAGGAGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA2940.21261824177731492No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2860.2068327113888166No Hit
GAGGAGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC2760.19960079840319359No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG2720.19670803320894445No Hit
GAGGAGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC2500.18079782464057392No Hit
GAGGAGGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC2410.17428910295351327No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2340.1692267638635772No Hit
GAGGAGGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC2220.16054846828082964No Hit
GAGGAGGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT2140.15476293789233128No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG2040.14753102490670833No Hit
GAGGAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC2030.14680783360814603No Hit
GAGGAGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC1830.1323440076369001No Hit
GAGGAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC1830.1323440076369001No Hit
GAGGAGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT1800.1301744337412132No Hit
GAGGAGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT1760.12728166854696404No Hit
GAGGAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC1720.12438890335271487No Hit
GAGGAGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT1620.1171569903670919No Hit
GAGGAGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG1580.11426422517284274No Hit
GAGGAGGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCG1520.10992507738146894No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1500.10847869478434437No Hit
GAGGAGGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG1500.10847869478434437No Hit
GAGGAGGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA1460.10558592959009516No Hit
GAGGAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1450.10486273829153286No Hit
GAGGAGGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG1450.10486273829153286No Hit
GAGGAGGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG1420.102693164395846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAA150.00800772250.63163838
ATCCGTG203.920252E-450.63163838
CCTTCGG150.00804281250.5756937
TTGTTTA150.00804281250.5756937
GACGACA150.00812513450.4456336
ACGGGGC150.00813694550.42710535
AAGAGAC354.9036316E-850.427135
ATATGCC1100.050.3346834
TCCACGA252.0191024E-550.24258845
CAACGAT150.00826771350.22421646
ACGGCAA150.00826771350.22421646
ATTATCT204.0798058E-450.22421646
AACGATT150.00830364650.16916747
CACGATT252.036661E-550.16916347
ACATATC150.00838794350.0411812
GATTACG150.00842426449.9865350
GCAAAAC204.1915194E-449.9501659
GGAGTCG252.0900969E-549.9501659
GGCCTAC204.1915194E-449.9501659
GGTAGAG650.049.87758632