##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525898_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	138276
Sequences flagged as poor quality	0
Sequence length	56
%GC	56
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.54086754028175	39.0	36.0	39.0	30.0	40.0
2	35.106779195232725	38.0	35.0	39.0	23.0	40.0
3	36.32780091989933	39.0	36.0	39.0	31.0	40.0
4	36.15994821950302	39.0	36.0	39.0	30.0	40.0
5	34.50224189302554	38.0	35.0	39.0	20.0	40.0
6	36.103271717434694	39.0	36.0	39.0	30.0	40.0
7	36.22727732939917	38.0	36.0	39.0	31.0	40.0
8	35.018296739853625	38.0	35.0	39.0	27.0	40.0
9	35.117106366976195	38.0	35.0	39.0	27.0	40.0
10	34.66884347247534	38.0	35.0	39.0	25.0	40.0
11	34.39795047585987	38.0	33.0	39.0	26.0	40.0
12	33.76025485261361	38.0	32.0	39.0	22.0	40.0
13	33.48970898782146	38.0	32.0	39.0	20.0	40.0
14	34.079782464057395	38.0	33.0	39.0	23.0	40.0
15	33.12487344152275	38.0	32.0	39.0	18.0	40.0
16	32.59873730799271	36.0	30.0	39.0	19.0	40.0
17	31.701676357430067	36.0	28.0	39.0	15.0	40.0
18	31.040686742457115	35.0	27.0	38.0	14.0	40.0
19	32.139575920622526	36.0	29.0	38.0	18.0	40.0
20	32.70534293731378	36.0	30.0	39.0	20.0	40.0
21	32.828654285631636	37.0	31.0	39.0	19.0	40.0
22	32.119746015215945	36.0	29.0	39.0	16.0	40.0
23	32.766206717000784	37.0	30.0	39.0	19.0	40.0
24	32.08171338482455	36.0	29.0	39.0	16.0	40.0
25	32.49367207613758	36.0	30.0	39.0	19.0	40.0
26	32.971014492753625	37.0	32.0	39.0	19.0	40.0
27	33.181658422285864	37.0	32.0	39.0	20.0	40.0
28	33.116556741589285	37.0	32.0	39.0	20.0	40.0
29	32.615479186554424	37.0	31.0	39.0	18.0	40.0
30	32.32271688507044	36.0	30.0	39.0	17.0	40.0
31	33.00109201886083	37.0	32.0	39.0	19.0	40.0
32	32.930544707686074	37.0	32.0	39.0	18.0	40.0
33	32.73092221354393	37.0	32.0	39.0	18.0	40.0
34	32.56512337643554	36.0	32.0	39.0	17.0	40.0
35	32.40901530272788	36.0	31.0	39.0	17.0	40.0
36	31.786492232925454	36.0	30.0	38.0	10.0	40.0
37	31.507853857502386	36.0	30.0	38.0	9.0	40.0
38	30.815803176256182	35.0	29.0	38.0	4.0	39.0
39	29.751742891029537	35.0	27.0	38.0	4.0	39.0
40	27.69440105296653	33.0	19.0	38.0	4.0	39.0
41	25.739419711302034	31.0	15.0	37.0	4.0	39.0
42	26.638664699586336	31.0	19.0	36.0	4.0	39.0
43	26.631917324770747	32.0	19.0	37.0	4.0	39.0
44	25.704865631056727	31.0	15.0	36.0	4.0	39.0
45	26.51435534727646	32.0	19.0	37.0	4.0	39.0
46	24.533331886950737	29.0	12.0	35.0	4.0	39.0
47	23.66247938904799	28.0	9.0	35.0	4.0	38.0
48	24.200569874743266	29.0	10.0	35.0	4.0	38.0
49	23.19202175359426	28.0	5.0	35.0	4.0	38.0
50	23.739152130521564	28.0	4.0	35.0	4.0	38.0
51	23.920340478463363	30.0	4.0	35.0	4.0	38.0
52	23.69542798461049	29.0	4.0	35.0	4.0	38.0
53	23.486989788538864	29.0	4.0	35.0	4.0	38.0
54	23.157149469177586	29.0	4.0	35.0	4.0	38.0
55	22.815593450779602	29.0	4.0	35.0	4.0	38.0
56	22.221687060661285	28.0	4.0	35.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.6531849945337314
1	2	4.355517892159362
1	3	3.6017058655959033
1	4	3.5003804936675564
1	5	4.918578171804967
1	6	3.6596264142427515
1	7	3.901517844295192
1	8	4.507487706471203
1	9	4.496865523225587
1	10	4.208808572497581
1	11	-2.191037322458243
1	12	-8.676539466518438
1	13	-19.05761800920473
1	14	-7.7581319089042395
1	15	-0.23669259946263566
1	16	-2.4839236578601422
1	17	-6.099581286915754
1	18	-5.521302237339757
1	19	-0.9129156742399545
1	20	-1.6948674595192905
1	21	5.285186571373629
1	22	6.63622882598418
1	23	5.63793979328101
1	24	6.753835618356945
1	25	5.449630945013212
1	26	5.431459501887684
1	27	4.821891598273769
1	28	5.359611558518818
1	29	5.484036876168069
1	30	5.312434806134661
1	31	4.393705827542128
1	32	4.231584325750472
1	33	4.557495100538567
1	34	4.308866477965481
1	35	4.629254185319802
1	36	2.652304306235976
1	37	2.753702272918318
1	38	3.39070793153914
1	39	3.233821777374466
1	40	-11.2877372969622
1	41	-2.5363612109957003
1	42	-4.038213583682911
1	43	-10.689694450216523
1	44	-15.130706572254278
1	45	-4.9991920872999245
1	46	-7.064728254436126
1	47	-11.409898744058104
1	48	-7.093767305189923
1	49	-13.579596399539415
1	50	-5.705866392916336
1	51	-0.9354323328192997
1	52	-1.0473677711923735
1	53	-0.5433481633451187
1	54	-0.6722708892974083
1	55	-0.9406314184833064
1	56	2.2797507856829355
2	1	4.061569859851829
2	2	5.054165832626815
2	3	3.7242925965465403
2	4	3.706669142656132
2	5	4.8877009052710605
2	6	3.927753444952323
2	7	3.9560194688628485
2	8	5.109604884964021
2	9	5.174006284152114
2	10	4.61641783777376
2	11	3.279144000254263
2	12	-1.1113785819151474
2	13	0.6733535870640033
2	14	3.7595916001618974
2	15	4.774291574104076
2	16	3.9525924306001876
2	17	5.805963205275841
2	18	4.2919458498628416
2	19	5.1475392047035555
2	20	4.444405153414365
2	21	6.6936343231816124
2	22	7.376926866021915
2	23	5.993164612482349
2	24	6.861413440388176
2	25	5.930838365619017
2	26	5.371486752699969
2	27	5.1284841986301295
2	28	5.192186760279636
2	29	5.772036037681538
2	30	5.660343830345958
2	31	4.466842815279264
2	32	4.353039099883166
2	33	4.410655146655323
2	34	4.265411913454422
2	35	4.296249259211422
2	36	4.483286383586361
2	37	4.628977401311566
2	38	5.312749646244097
2	39	5.6456025131464
2	40	3.9445653966757845
2	41	8.213434407912171
2	42	7.04064607463383
2	43	6.789018472957149
2	44	-3.677567488300813
2	45	6.919495681277795
2	46	5.572228039453403
2	47	2.901179759243437
2	48	5.046825924031332
2	49	-2.3497253168436814
2	50	5.643656717868694
2	51	7.981599424858093
2	52	7.829269897815067
2	53	8.267510237241822
2	54	7.994472638634676
2	55	7.918817276622029
2	56	8.176071926024616
3	1	3.5669352622570116
3	2	4.01855066148898
3	3	3.5345608666667943
3	4	3.451997542286101
3	5	4.955138463087891
3	6	3.585477774277024
3	7	3.943068497539997
3	8	4.458633562114805
3	9	4.476411464540639
3	10	3.7116999839347216
3	11	3.4537045922986636
3	12	3.880110779786982
3	13	4.036813346545966
3	14	4.27161750210454
3	15	-1.2810275748320947
3	16	5.471741366770399
3	17	6.18773933848583
3	18	7.021640576967378
3	19	5.743006364740559
3	20	4.822481009103541
3	21	5.848583444365701
3	22	6.706436579250404
3	23	5.359122059290858
3	24	6.18842546629012
3	25	5.919367505304493
3	26	4.875002016344741
3	27	4.407670137575547
3	28	4.863509609493331
3	29	4.83299810983695
3	30	4.9334243113820335
3	31	3.4805338421062757
3	32	3.3142786928526355
3	33	4.050063102251997
3	34	3.450888324185229
3	35	4.004944911385337
3	36	4.48689415416915
3	37	4.693732257925809
3	38	5.00923438259932
3	39	5.637184381786547
3	40	7.2894312616156505
3	41	9.03489601753289
3	42	8.322977249471943
3	43	8.435364448905347
3	44	8.894587923355058
3	45	8.438182082757905
3	46	9.560202137510757
3	47	10.707192710214763
3	48	9.435725091468889
3	49	10.467758494442165
3	50	9.903878734519946
3	51	9.300378333276225
3	52	9.513266197537547
3	53	9.5565019116174
3	54	9.28309428525817
3	55	9.577466675326932
3	56	8.262380899197606
4	1	3.7887523806036683
4	2	4.91040092469818
4	3	3.691414054422694
4	4	3.695105360574267
4	5	5.027725712064303
4	6	3.9029275695587557
4	7	3.9374143739291583
4	8	4.906303673247578
4	9	5.170991304665421
4	10	4.825367365490578
4	11	5.180298470247578
4	12	5.297151138531376
4	13	5.72371137735119
4	14	4.754382173580467
4	15	5.414039689741898
4	16	6.973983519236764
4	17	8.16444044981358
4	18	8.2587238290633
4	19	7.214345807126989
4	20	6.524664670636
4	21	6.839517349610389
4	22	7.616789474551634
4	23	6.581543583954538
4	24	7.441281773562494
4	25	6.477332020804596
4	26	5.895883279748919
4	27	5.634809930907583
4	28	5.3956406291852765
4	29	5.733700001043815
4	30	5.517475783403874
4	31	4.3437820386358155
4	32	4.662475222390558
4	33	4.592508023289234
4	34	4.378340069164757
4	35	4.141989169525324
4	36	4.415983645298027
4	37	4.677159826652662
4	38	4.792837424127885
4	39	5.2516706364170815
4	40	-0.043928067688305816
4	41	8.89974296625584
4	42	8.382560014143419
4	43	8.502843489654026
4	44	9.585346355798034
4	45	7.988172334268921
4	46	11.15411908038461
4	47	10.288261144607429
4	48	10.843562876612197
4	49	11.023597839343704
4	50	11.03218834653008
4	51	10.79131223852091
4	52	10.895484953630351
4	53	10.897660032108725
4	54	10.537607835657859
4	55	10.845160182833268
4	56	10.268639429125468
5	1	3.4754248563225403
5	2	4.157866548569139
5	3	3.2661701804932974
5	4	3.231989299961768
5	5	4.738179880767682
5	6	3.1075053860635435
5	7	3.6038968511357155
5	8	4.376560617544435
5	9	4.513604953104419
5	10	4.053344327639643
5	11	4.245565297017393
5	12	4.5578358982703975
5	13	5.0167638925999505
5	14	4.691708167672232
5	15	5.88958853891198
5	16	6.443305347813055
5	17	6.947578613161294
5	18	7.648263284570916
5	19	6.72950955864124
5	20	5.803626692186171
5	21	5.968818350609261
5	22	6.609053517226915
5	23	5.862439594835887
5	24	6.025495319988863
5	25	5.920109314494688
5	26	4.878031070538025
5	27	4.3930400118801
5	28	4.0961698196726
5	29	4.563041382039714
5	30	4.709068242970744
5	31	3.7564868608575637
5	32	3.4905201929556675
5	33	3.979879710535535
5	34	3.622596545903754
5	35	4.074448311344128
5	36	5.08984078511908
5	37	4.7308227174353945
5	38	4.716157935041114
5	39	5.509613806884424
5	40	6.267465467261648
5	41	8.242314109434808
5	42	8.06668217426073
5	43	7.9281936267334885
5	44	8.461103480742242
5	45	8.059921213192688
5	46	8.47992189848335
5	47	9.136432355794813
5	48	8.413388392519789
5	49	8.989147326092692
5	50	8.640268596461013
5	51	8.347153523879975
5	52	7.860226840438845
5	53	8.172945348666648
5	54	7.843943244664725
5	55	8.047485220556688
5	56	7.585006079498449
6	1	3.9576184920706723
6	2	5.10198751449105
6	3	3.79004740910986
6	4	3.845372283486931
6	5	5.642716102839444
6	6	4.191481915063775
6	7	3.9803355782853416
6	8	5.135238116980233
6	9	5.0205108434226275
6	10	4.90962959055998
6	11	5.196975813830754
6	12	5.599158398834604
6	13	5.811347508036651
6	14	5.163542967943052
6	15	6.390400996596476
6	16	6.928884223913254
6	17	8.049022982378172
6	18	8.683131260197186
6	19	7.294145081096666
6	20	6.395772934569379
6	21	7.008831891570672
6	22	7.671861009892229
6	23	6.901979202148002
6	24	7.690288820327389
6	25	6.52040270199187
6	26	6.224070339326108
6	27	6.000216721426479
6	28	5.9732404583546135
6	29	6.582066432818316
6	30	6.1795940836387615
6	31	4.698960343141472
6	32	4.631255918525515
6	33	4.935984427303758
6	34	4.171592257932637
6	35	4.610534973497138
6	36	5.493519412968325
6	37	5.545492092294339
6	38	5.683975583427479
6	39	6.5661698890329205
6	40	3.566449895156026
6	41	10.164295603454217
6	42	9.410554889223494
6	43	9.199960722197268
6	44	10.27947405171945
6	45	9.90277835604983
6	46	11.253990957386534
6	47	11.643631633610205
6	48	11.295559459998913
6	49	12.003162221150237
6	50	11.449634428101728
6	51	11.074912494766906
6	52	10.946392491533409
6	53	11.359607501679513
6	54	11.094067370144298
6	55	10.478087793339355
6	56	9.924906779114789
7	1	4.00179838625057
7	2	4.8785748160783555
7	3	3.6825077265701793
7	4	3.620636833751483
7	5	5.234647319770666
7	6	3.907619481201337
7	7	3.852690075639835
7	8	4.81354499528711
7	9	5.183738356650146
7	10	4.3862168921472815
7	11	5.065097506952448
7	12	5.343073742749944
7	13	5.732908354597495
7	14	5.435103814503897
7	15	6.413416869612348
7	16	7.31896358899262
7	17	8.042409225764413
7	18	8.316729143795072
7	19	6.915309102260686
7	20	6.605693569490015
7	21	6.815974748713529
7	22	7.322257835289054
7	23	6.832270207439006
7	24	7.242934322972893
7	25	6.50664608823525
7	26	5.668117958373731
7	27	5.608153229362987
7	28	5.332631992746148
7	29	5.74833627408816
7	30	6.0791972582419405
7	31	4.355574099755231
7	32	4.9915733788429755
7	33	5.02659289291222
7	34	4.37053405687444
7	35	4.570323333285501
7	36	5.812699307148222
7	37	5.918772515574766
7	38	6.571673996125892
7	39	7.003206926069964
7	40	6.911291164997298
7	41	10.394718883877498
7	42	9.640449069117675
7	43	9.52377024600679
7	44	10.519950242195637
7	45	9.758995287266764
7	46	11.2680121214077
7	47	11.92550994048851
7	48	10.625056814496268
7	49	11.726574919562939
7	50	10.803073581540875
7	51	10.267769637624049
7	52	10.408958629099544
7	53	10.432226549298559
7	54	10.216025042101972
7	55	10.31538938064094
7	56	9.071599900807911
8	1	3.7074442760428
8	2	4.770512485455043
8	3	3.486098512478037
8	4	3.6508310523603313
8	5	5.551426904233175
8	6	3.667556247235666
8	7	3.9078617106895237
8	8	4.8640418335888285
8	9	5.038977742377334
8	10	4.550512285101213
8	11	4.6542348149921935
8	12	5.133769316372344
8	13	5.632253431381596
8	14	5.084448891287998
8	15	5.821411449558148
8	16	6.91564380579479
8	17	7.732065955665046
8	18	8.19428108598116
8	19	6.487757160074594
8	20	6.184623108785409
8	21	6.424511334079384
8	22	6.712162984340541
8	23	5.822536693437204
8	24	6.974957809874425
8	25	6.0341076961846625
8	26	5.268321210406246
8	27	5.2664188120188165
8	28	4.418652769620582
8	29	5.436073401459431
8	30	5.571812434393699
8	31	3.9151043617245165
8	32	4.1067495306044925
8	33	4.500328213598763
8	34	4.2798384832520355
8	35	3.9547858445772803
8	36	4.917328936777849
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115	27	4.564502435712196
115	28	4.8684785535927055
115	29	4.853495004246888
115	30	4.577213131257814
115	31	3.294198438379567
115	32	3.250303537573135
115	33	3.2121749034942297
115	34	3.119211305551687
115	35	4.0819635449257134
115	36	4.167328936777849
115	37	4.007396854199101
115	38	4.517308916760815
115	39	4.604265127128162
115	40	6.952164180870309
115	41	7.890486079644695
115	42	7.010554889223496
115	43	5.042817865054413
115	44	-0.8395735673281699
115	45	6.1742069274784015
115	46	-0.1317233283277517
115	47	-8.725415985437413
115	48	-0.6353929209534712
115	49	-5.032552064564047
115	50	0.3091582376255353
115	51	1.7034839233383359
115	52	2.265440110581025
115	53	2.2953217873937994
115	54	2.632162608239536
115	55	2.2066592219107832
115	56	5.191573445781454
116	1	3.907532433550884
116	2	5.3804155121961585
116	3	3.6917112071592015
116	4	4.029996494617166
116	5	5.821717824009845
116	6	3.767615018907719
116	7	4.214414752123552
116	8	4.9579001938591745
116	9	5.099512564593027
116	10	4.67922224689962
116	11	3.7083929107900175
116	12	3.1270987315942094
116	13	4.533722723182951
116	14	4.6124242071857395
116	15	-3.54608781579595
116	16	3.821138957131847
116	17	5.855104451110247
116	18	4.9344221379940905
116	19	5.234363096013475
116	20	4.832433864001395
116	21	5.89730004991835
116	22	6.955624635824531
116	23	5.836230493025798
116	24	6.069462658537368
116	25	6.117534084665415
116	26	5.577254504558464
116	27	4.897979199911848
116	28	4.6161549735582845
116	29	5.48344336913501
116	30	5.693822425577949
116	31	4.782838713766829
116	32	4.405897341359708
116	33	4.848491598847069
116	34	4.749159670439809
116	35	4.207092632705404
116	36	5.5101000211152
116	37	6.174350382598412
116	38	6.926259002819332
116	39	7.518378724374287
116	40	8.956639223899575
116	41	7.547887112346931
116	42	5.823291550152927
116	43	2.0438505672919334
116	44	0.17557273215548008
116	45	5.885222418011963
116	46	0.9133713360440225
116	47	-6.598307551702474
116	48	1.440596752024156
116	49	-1.8877155757516526
116	50	1.5288656386582389
116	51	4.7225889147324835
116	52	4.897970231062953
116	53	4.540158276206192
116	54	4.829925086724906
116	55	4.283595538606136
116	56	6.555256750428615
117	1	3.10276134921353
117	2	4.54846370496724
117	3	2.9799521710146237
117	4	3.117562759677405
117	5	5.273573245696589
117	6	3.0004342960161523
117	7	3.2797641497139125
117	8	3.60628573602785
117	9	3.7673679862797727
117	10	3.2814391143695048
117	11	-0.902643233788293
117	12	-7.407889220213018
117	13	-8.805795349106209
117	14	-2.065599889199806
117	15	-8.297027574832093
117	16	-7.968258633229603
117	17	0.4522610776162672
117	18	-2.362011596945667
117	19	1.2280498430014255
117	20	-0.059084208287764284
117	21	4.853974748713526
117	22	5.571480057511277
117	23	4.967122059290862
117	24	4.762860248898818
117	25	4.890497940087101
117	26	5.417784625040394
117	27	5.101931007140767
117	28	4.567335696449849
117	29	5.223780718532602
117	30	5.498641702686385
117	31	4.42905558123671
117	32	4.40801782328742
117	33	3.765889189208515
117	34	4.693497019837402
117	35	3.936249259211422
117	36	4.397328936777846
117	37	4.946253997056246
117	38	4.874451773903669
117	39	5.952836555699591
117	40	7.624735609441739
117	41	5.986200365358979
117	42	4.965412032080636
117	43	-5.030896420659875
117	44	-1.6287164244710262
117	45	4.675921213192687
117	46	-0.8265804711848936
117	47	-1.911415985437415
117	48	0.1894642219036733
117	49	-1.4659806359926186
117	50	0.4094439519112498
117	51	4.41376963762405
117	52	4.773440110581024
117	53	3.6618932159652253
117	54	4.629876893953824
117	55	4.4209449361964985
117	56	6.029859160067172
118	1	2.937533626441258
118	2	3.961770635660308
118	3	2.5390808838859087
118	4	3.1740380072021566
118	5	4.666761364508474
118	6	2.8387709296795194
118	7	3.165625535852527
118	8	3.3513154389981494
118	9	3.4198036298441252
118	10	2.7779935698150453
118	11	-1.174405610025918
118	12	-8.902067438034802
118	13	-7.113122081779476
118	14	-9.87874840405129
118	15	-8.82558203027764
118	16	-9.49855566293257
118	17	-1.9696003085223452
118	18	-8.020546250411016
118	19	-1.9627620381866926
118	20	-1.5272822280897458
118	21	3.585776728911547
118	22	4.350173126818209
118	23	4.153181465231452
118	24	5.0822265855324815
118	25	4.185230613354435
118	26	4.328596506228511
118	27	4.212663680408095
118	28	3.871731736053814
118	29	4.627186659126664
118	30	4.859037742290347
118	31	3.8766595416327476
118	32	4.163740595564644
118	33	4.395908991188712
118	34	3.5612197921146276
118	35	3.951694803765882
118	36	5.396536857569927
118	37	5.323521323788917
118	38	5.801184447171
118	39	6.7813514071847365
118	40	7.946082144095204
118	41	6.220259771299574
118	42	5.992025893466774
118	43	-0.1131934503628429
118	44	2.296352882459665
118	45	4.861901411212489
118	46	-1.599332946432419
118	47	-2.900168460684938
118	48	-1.48610013453197
118	49	-2.4094261805470722
118	50	0.11898850636669422
118	51	3.6761854792082076
118	52	3.7357371402839945
118	53	3.5184476714107724
118	54	4.258470953359765
118	55	3.700152856988577
118	56	6.413779952146378
119	1	2.8840526877962063
119	2	4.341282602605034
119	3	2.669275005660289
119	4	2.563295043141977
119	5	4.691085056720212
119	6	2.5354264220004055
119	7	3.0714491890839923
119	8	3.3456243187050134
119	9	3.622423104390009
119	10	3.1310769096450954
119	11	-0.8734778794575853
119	12	-7.718314417063411
119	13	-4.32611030973613
119	14	-6.952655007310042
119	15	-6.20228741735178
119	16	-9.638494853702042
119	17	-0.2297546704152289
119	18	-5.209444667811809
119	19	-0.7258399207780997
119	20	-0.7990527122247713
119	21	3.857313331390692
119	22	4.907669033889231
119	23	3.779594500235742
119	24	3.355868122914565
119	25	4.000230223551675
119	26	4.981438168347484
119	27	4.626088487455728
119	28	3.974327822434102
119	29	4.591402765776699
119	30	4.906515718434413
119	31	3.91579573871703
119	32	3.715545382342537
119	33	4.253007299444739
119	34	3.9712922954279506
119	35	4.078926424565758
119	36	5.254336810793596
119	37	4.807734312016876
119	38	5.364215553431226
119	39	6.121025532077546
119	40	7.394121436213393
119	41	4.508090129138505
119	42	4.2156167564900855
119	43	-4.44210901908507
119	44	-2.9478975268332306
119	45	2.7764566462635543
119	46	-1.99584031370458
119	47	-2.8321088988232397
119	48	-5.55119719541916
119	49	-4.316185360402066
119	50	-1.129705654387962
119	51	2.8169822360492454
119	52	3.0174086145180326
119	53	3.6431845545479042
119	54	2.4438611459223267
119	55	3.2114961172988608
119	56	6.300347349043548
120	1	2.808511349213532
120	2	3.8455887049672413
120	3	2.712889671014622
120	4	2.577812759677407
120	5	4.34807324569659
120	6	2.8708717960161536
120	7	2.942389149713911
120	8	3.4136607360278504
120	9	3.157117986279772
120	10	2.741251614369503
120	11	-1.8943932337882927
120	12	-7.948014220213018
120	13	-8.064545349106208
120	14	-7.026412389199805
120	15	-1.1638400748320947
120	16	-9.715758633229601
120	17	-1.2073014223837326
120	18	-7.384949096945668
120	19	-1.6095751569985737
120	20	-1.6720842082877638
120	21	1.7144122487135292
120	22	4.505417557511276
120	23	3.8403720592908606
120	24	2.8251102488988167
120	25	2.842872940087105
120	26	4.576972125040395
120	27	4.151556007140766
120	28	3.95821069644985
120	29	4.528718218532603
120	30	4.179891702686383
120	31	3.4620555812367115
120	32	2.8735178232874183
120	33	3.0885141892085173
120	34	3.5756845198374023
120	35	3.5040617592114245
120	36	4.004828936777848
120	37	4.228378997056247
120	38	3.79476427390367
120	39	5.3593990556995905
120	40	5.543235609441741
120	41	-7.881612134641021
120	42	-6.107525467919363
120	43	-8.846021420659874
120	44	-15.310778924471027
120	45	-7.166641286807312
120	46	-10.082392971184895
120	47	-7.914478485437414
120	48	-7.733160778096327
120	49	-14.157105635992618
120	50	-7.092181048088751
120	51	-2.880667862375951
120	52	-3.179872389418975
120	53	-2.5964192840347735
120	54	-2.627435606046177
120	55	-2.605617563803502
120	56	0.4089841600671704
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	3230.0
5	159.0
6	408.0
7	302.0
8	527.0
9	747.0
10	458.0
11	408.0
12	409.0
13	404.0
14	509.0
15	574.0
16	750.0
17	896.0
18	1089.0
19	1416.0
20	1851.0
21	2333.0
22	2776.0
23	3348.0
24	3823.0
25	4249.0
26	4479.0
27	4685.0
28	5139.0
29	5509.0
30	6184.0
31	6705.0
32	7241.0
33	8236.0
34	9202.0
35	9839.0
36	10688.0
37	11117.0
38	10521.0
39	8065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.46049929127253	0.41149584888194624	0.08244380803610171	0.04556105180942463
8	98.82987647892621	0.7940640458214008	0.261795250079551	0.11426422517284274
9	97.6177561779957	1.0059809217996283	0.7948044809905044	0.5814584192141633
10	57.80178772889004	17.82594231826203	13.63432555179496	10.737944401052966
11	38.28357777199225	27.18331453035957	16.203824235586797	18.329283462061387
12	31.137724550898206	17.676241719459632	19.75686308542336	31.429170644218807
13	24.15892851977205	14.62582082212387	23.337383204605285	37.8778674534988
14	22.463768115942027	23.568081228846655	29.367352252017703	24.600798403193615
15	31.182639514278243	18.867472329547105	23.231560293989485	26.718327862185166
16	32.01050734863122	22.268053173552165	22.871574437907505	22.84986503990911
17	27.947803568974372	19.62284895874775	31.680976802389928	20.748370669887954
18	25.015187017269806	18.966414996094766	27.86094477711244	28.15745320952298
19	31.059630438871928	21.79383293058791	19.970626112373207	27.17591051816695
20	27.74873280231716	25.53801593048516	24.105720492396816	22.607530774800868
21	24.645447773607284	22.364452930073117	26.617633232807563	26.37246606351204
22	23.557233359368222	19.49579102664237	27.855159246723943	29.091816367265473
23	23.041597963493306	23.63533801961295	24.182793832624604	29.14027018426914
24	21.692654844215582	23.66563485405155	26.631613967078426	28.01009633465444
25	29.707739373820218	22.694496879226424	23.170387728091303	24.427376018862056
26	29.093106043065525	22.067463533225286	22.08555221115999	26.753878212549203
27	24.6849963451615	23.41195457868035	19.877979055242342	32.025070020915805
28	25.82644478676242	26.7969885660975	22.63999479290968	24.736571854230398
29	33.94679471858274	22.60319115804417	19.643801287761917	23.80621283561118
30	29.59926481229558	20.976005094214013	26.99571622912385	22.42901386436655
31	29.528900333451475	25.98100556234675	22.78175203072672	21.708342073475055
32	33.68751491830195	22.885579336433928	20.629570425234895	22.797335320029223
33	28.896917218111522	23.537162798106024	21.005343102474626	26.560576881307828
34	25.0699062604135	25.907332550962753	27.25040205154953	21.772359137074222
35	25.049971031286212	24.298232908458864	31.142091541135574	19.50970451911935
36	28.50651558893544	25.984935640018232	23.03790690775431	22.470641863292016
37	27.91868246472957	21.270170051276107	28.464034299950754	22.34711318404357
38	31.742788200256516	24.813953319855365	21.351709745440317	22.0915487344478
39	28.848911484812934	22.666898419733776	20.095887831515522	28.388302263937774
40	24.099979578142776	18.67214166934096	24.93217025994107	32.2957084925752
41	21.223368501037495	16.351521173685597	22.322237484291435	40.102872840985476
42	26.720856204363685	24.01331293370362	23.32228756226842	25.943543299664277
43	20.972664206856056	19.407615747620532	29.738820305760743	29.88089973976267
44	22.439215629333315	22.792051749297595	23.98487015485585	30.78386246651324
45	25.965085185480426	22.430123267122244	28.2036045306646	23.401187016732734
46	21.86269461989746	25.413170699870193	24.94615546386071	27.77797921637164
47	19.250719204951615	25.387934792084387	24.39121843489379	30.970127568070204
48	24.13566042923921	25.263752007988536	25.438139481034455	25.1624480817378
49	21.924490284049316	25.639092839695877	22.661612391189326	29.774804485065488
50	26.857875595867686	23.4992827854007	24.998188852021965	24.64465276670965
51	24.02901178603808	27.06291931097008	25.70589301903898	23.202175883952854
52	23.34108375526051	25.186698247825174	27.646551786580904	23.825666210333413
53	23.878854673463024	23.52719389197851	27.614434768737716	24.979516665820746
54	24.668048589568897	25.849447714028933	25.540689415243673	23.941814281158496
55	23.049192694355387	26.229817358884716	25.60312187196263	25.11786807479727
56	25.593904728764777	21.611825174166142	27.534561376800564	25.259708720268513
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.5
9	2.0
10	7.0
11	12.0
12	54.5
13	113.5
14	130.0
15	128.5
16	127.0
17	117.0
18	107.0
19	129.0
20	151.0
21	165.5
22	186.0
23	192.0
24	219.0
25	246.0
26	264.5
27	283.0
28	331.5
29	419.0
30	458.0
31	526.5
32	595.0
33	663.0
34	731.0
35	833.5
36	936.0
37	1152.0
38	1508.0
39	1648.0
40	1743.0
41	1838.0
42	2095.5
43	2353.0
44	2872.5
45	3392.0
46	4502.0
47	8390.5
48	11169.0
49	10679.5
50	10190.0
51	8828.5
52	7467.0
53	7454.0
54	7568.0
55	7695.0
56	7985.0
57	8275.0
58	8005.5
59	7736.0
60	8291.5
61	8847.0
62	9346.0
63	9226.5
64	8608.0
65	7899.5
66	7191.0
67	6997.0
68	6803.0
69	5632.5
70	4462.0
71	4176.0
72	3343.5
73	2797.0
74	2576.0
75	2355.0
76	1717.5
77	1080.0
78	824.5
79	488.0
80	407.0
81	420.5
82	434.0
83	282.0
84	130.0
85	84.5
86	39.0
87	23.0
88	3.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0021695738956868872
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.42234371836038065
16	0.06291764297491972
17	0.020972547658306574
18	0.0
19	0.04194509531661315
20	0.12728166854696404
21	0.0021695738956868872
22	0.0
23	0.0
24	0.005785530388498366
25	0.006508721687060662
26	0.04917700830223611
27	0.07448870375191646
28	0.0021695738956868872
29	0.14970059880239522
30	0.05785530388498365
31	0.018079782464057392
32	0.0166333998669328
33	0.11137145997859353
34	0.1692267638635772
35	0.14174549451820995
36	0.049900199600798396
37	0.14463825971245914
38	0.19743122450750672
39	0.14319187711533454
40	0.8446874367207613
41	1.0182533483757124
42	0.26396482397523796
43	0.23503717203274607
44	0.38690734473082816
45	0.20466313749312967
46	0.2740895021551101
47	0.4512713703028725
48	0.056408921287859064
49	0.2220197286586248
50	0.17501229425207557
51	0.2899997107234806
52	0.15837889438514277
53	0.2596256761838642
54	0.21985015476293787
55	0.29650843241054126
56	0.24082270242124448
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	138276.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.64135497121698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.54681005762407	59.00879400619051
2	6.576305765662134	8.765078538575024
3	1.9153761842233774	3.829297925887356
4	0.9343563142302141	2.4906708322485467
5	0.46880595557195415	1.5620932048945588
6	0.3071113088584792	1.227978824958778
7	0.20727300350519268	0.9669067661777893
8	0.15952424877101215	0.8504729671092597
9	0.10851989712313753	0.6508721687060661
>10	0.6619713724511389	8.151812317394198
>50	0.06294154033141976	2.930371141774422
>100	0.044493157820486386	5.261939888339263
>500	0.005425994856156876	2.403164685122509
>1k	0.0010851989712313754	1.9005467326217131
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	2628	1.9005467326217131	No Hit
GAGGAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	737	0.532991987040412	No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	703	0.5084034828892939	No Hit
GAGGAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	668	0.4830917874396135	No Hit
GAGGAGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	651	0.47079753536405455	No Hit
GAGGAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	564	0.4078798923891348	No Hit
GAGGAGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	359	0.2596256761838642	No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	340	0.24588504151118054	No Hit
GAGGAGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	294	0.21261824177731492	No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	286	0.2068327113888166	No Hit
GAGGAGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	276	0.19960079840319359	No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	272	0.19670803320894445	No Hit
GAGGAGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	250	0.18079782464057392	No Hit
GAGGAGGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	241	0.17428910295351327	No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	234	0.1692267638635772	No Hit
GAGGAGGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC	222	0.16054846828082964	No Hit
GAGGAGGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT	214	0.15476293789233128	No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	204	0.14753102490670833	No Hit
GAGGAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	203	0.14680783360814603	No Hit
GAGGAGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC	183	0.1323440076369001	No Hit
GAGGAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC	183	0.1323440076369001	No Hit
GAGGAGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT	180	0.1301744337412132	No Hit
GAGGAGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	176	0.12728166854696404	No Hit
GAGGAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC	172	0.12438890335271487	No Hit
GAGGAGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	162	0.1171569903670919	No Hit
GAGGAGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	158	0.11426422517284274	No Hit
GAGGAGGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCG	152	0.10992507738146894	No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	150	0.10847869478434437	No Hit
GAGGAGGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG	150	0.10847869478434437	No Hit
GAGGAGGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA	146	0.10558592959009516	No Hit
GAGGAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	145	0.10486273829153286	No Hit
GAGGAGGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	145	0.10486273829153286	No Hit
GAGGAGGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG	142	0.102693164395846	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0043391477913737745	0.0	0.0	0.0	0.0
11	0.007231912985622957	0.0	0.0	0.0	0.0
12	0.007231912985622957	0.0	0.0	0.0	0.0
13	0.008678295582747549	0.0	0.0	0.0	0.0
14	0.014463825971245913	0.0	0.0	0.0	0.0
15	0.014463825971245913	0.0	0.0	0.0	0.0
16	0.01518701726980821	0.0	0.0	0.0	0.0
17	0.018802973762619687	0.0	0.0	0.0	0.0
18	0.02169573895686887	0.0	0.0	0.0	0.0
19	0.023142121553993464	0.0	0.0	0.0	0.0
20	0.026034886748242645	0.0	0.0	0.0	0.0
21	0.03254360843530331	0.0	0.0	0.0	0.0
22	0.04049871271948856	0.0	0.0	0.0	0.0
23	0.048453817003673814	0.0	0.0	0.0	0.0
24	0.0658104081691689	0.0	0.0	0.0	0.0
25	0.0687031733634181	0.0	0.0	0.0	0.0
26	0.07521189505047875	0.0	0.0	0.0	0.0
27	0.0838901906332263	0.0	0.0	0.0	0.0
28	0.09763082530590991	0.0	0.0	0.0	0.0
29	0.10269316439584598	0.0	0.0	0.0	0.0
30	0.10847869478434435	0.0	0.0	0.0	0.0
31	0.11137145997859353	0.0	0.0	0.0	0.0
32	0.11281784257571813	0.0	0.0	0.0	0.0
33	0.11354103387428043	0.0	0.0	0.0	0.0
34	0.1157106077699673	0.0	0.0	0.0	0.0
35	0.1164337990685296	0.0	0.0	0.0	0.0
36	0.1186033729642165	0.0	0.0	0.0	0.0
37	0.12004975556134109	0.0	0.0	0.0	0.0
38	0.12149613815846567	0.0	0.0	0.0	0.0
39	0.12221932945702797	0.0	0.0	0.0	0.0
40	0.12438890335271487	0.0	0.0	0.0	0.0
41	0.12800485984552634	0.0	0.0	0.0	0.0
42	0.1323440076369001	0.0	0.0	0.0	0.0
43	0.13885272932396078	0.0	0.0	0.0	0.0
44	0.14897740750383293	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAA	15	0.008007722	50.631638	38
ATCCGTG	20	3.920252E-4	50.631638	38
CCTTCGG	15	0.008042812	50.57569	37
TTGTTTA	15	0.008042812	50.57569	37
GACGACA	15	0.008125134	50.44563	36
ACGGGGC	15	0.008136945	50.427105	35
AAGAGAC	35	4.9036316E-8	50.4271	35
ATATGCC	110	0.0	50.33468	34
TCCACGA	25	2.0191024E-5	50.242588	45
CAACGAT	15	0.008267713	50.224216	46
ACGGCAA	15	0.008267713	50.224216	46
ATTATCT	20	4.0798058E-4	50.224216	46
AACGATT	15	0.008303646	50.169167	47
CACGATT	25	2.036661E-5	50.169163	47
ACATATC	15	0.008387943	50.04118	12
GATTACG	15	0.008424264	49.98653	50
GCAAAAC	20	4.1915194E-4	49.950165	9
GGAGTCG	25	2.0900969E-5	49.950165	9
GGCCTAC	20	4.1915194E-4	49.950165	9
GGTAGAG	65	0.0	49.877586	32
>>END_MODULE