FastQCFastQC Report
Fri 17 Jun 2016
SRR1525897_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525897_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3853
Sequences flagged as poor quality0
Sequence length40
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT160.41526083571243183No Hit
GAGGAGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAA110.28549182455229694No Hit
GAGGAGGGGACACGCAGGGATGAGATGGGCAAAAAAAAAA100.25953802232026996No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT90.23358422008824295No Hit
GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT90.23358422008824295No Hit
GAGGAGGGGACACGCAGGGATGAGATGGGGAAAAAAAAAA90.23358422008824295No Hit
GAGGAGGGGGATCCAGTGACAGATACCATTAGATTCAAGA70.18167661562418894No Hit
GAGGAGGGGGAGTGCAGTGCTGAGGAGGGGACACGCAGGG70.18167661562418894No Hit
GAGGAGGGGGTGCAGTGCTGAGGAGGGGACACGCAGGGAT60.15572281339216196No Hit
GAGGAGGGGGTGCAGTGCTGAGGAGGGGACACGCCGGGAT60.15572281339216196No Hit
GAGGAGGGGACACGCAGGGATGAGATGGCGAAAAAAAAAA50.12976901116013498No Hit
GAGGAGGGGCCACCACACCTGGCTTATAGTGAGTCCTTTG50.12976901116013498No Hit
GAGGAGGGGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTT50.12976901116013498No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC50.12976901116013498No Hit
GAGGAGGGGGAGTGCAGTGCTGAGGAGGGGACACGCCGGG50.12976901116013498No Hit
GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCCACTTTT40.10381520892810796No Hit
GAGGAGGGGAGCCTTTTAACCTGGAGACTTGTCGCCTTAT40.10381520892810796No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT40.10381520892810796No Hit
GAGGAGGGGGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAA40.10381520892810796No Hit
GAGGAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT40.10381520892810796No Hit
GAGGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG40.10381520892810796No Hit
GAGGAGGGGGACTAAGTTGTCCCGAGAACTGACTTTCTCC40.10381520892810796No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTG200.002524954533.8181845
AGGGGGA301.1797429E-533.8181845
AGGGGCA357.9716665E-733.8181845
AGGGGAT200.002524954533.8181845
AGGGGAG357.9716665E-733.8181845
AGGGGAA502.3283064E-1033.8181845
GGGGAAG301.1797429E-533.8181846
GAGGGGC700.031.4025974
GAGGGGA1300.031.2167824
GGAGGGG3800.030.703353
AGGAGGG3950.030.3935572
GAGGAGG4200.030.1948051
GAGGGGG1250.029.764
AGGGGGT402.265957E-629.590915
AGGGGGG402.265957E-629.590915
GGGGGAG305.052797E-428.181826
GAGGGGT653.8835424E-926.0139854
GGGGGTG350.00124472524.1558446
TTTTTTT5455.1901134E-65.58465434
Produced by FastQC (version 0.11.5)