##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525897_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3853 Sequences flagged as poor quality 0 Sequence length 40 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37243706202959 33.0 32.0 36.0 28.0 38.0 2 23.262652478588112 25.0 17.0 31.0 10.0 35.0 3 27.437581105631974 30.0 23.0 35.0 12.0 37.0 4 27.973786659745652 32.0 24.0 35.0 10.0 38.0 5 23.100441214637943 25.0 15.0 32.0 8.0 35.0 6 28.48222164547106 33.0 27.0 35.0 4.0 37.0 7 26.676615624188944 31.0 22.0 35.0 4.0 37.0 8 23.849987023098883 27.0 21.0 31.0 4.0 32.0 9 23.448222164547108 27.0 19.0 30.0 4.0 32.0 10 20.330391902413705 23.0 10.0 29.0 4.0 32.0 11 23.45315338697119 27.0 13.0 33.0 4.0 36.0 12 23.593563457046457 27.0 13.0 33.0 4.0 37.0 13 24.677653776278223 29.0 15.0 35.0 4.0 38.0 14 25.311964702828963 30.0 17.0 35.0 4.0 38.0 15 23.537243706202958 28.0 12.0 34.0 4.0 37.0 16 21.10848689332987 24.0 4.0 33.0 4.0 36.0 17 22.20166104334285 27.0 4.0 33.0 4.0 36.0 18 21.656890734492602 26.0 4.0 33.0 4.0 36.0 19 18.92369582143784 19.0 4.0 30.0 4.0 35.0 20 20.496496236698675 24.0 4.0 32.0 4.0 36.0 21 17.974824811834935 18.0 4.0 30.0 4.0 35.0 22 17.8629639242149 17.0 4.0 30.0 4.0 35.0 23 17.638463534907864 18.0 4.0 30.0 4.0 34.0 24 17.398131326239294 17.0 4.0 30.0 4.0 35.0 25 16.674020243965742 15.0 4.0 30.0 4.0 34.0 26 14.527900337399428 4.0 4.0 27.0 4.0 33.0 27 14.468466130288087 4.0 4.0 28.0 4.0 34.0 28 14.231248377887361 4.0 4.0 27.0 4.0 33.0 29 13.208928107967818 4.0 4.0 25.0 4.0 33.0 30 12.378146898520633 4.0 4.0 23.0 4.0 32.0 31 12.129509473137814 4.0 4.0 23.0 4.0 33.0 32 11.405398390864262 4.0 4.0 20.0 4.0 31.0 33 11.18271476771347 4.0 4.0 20.0 4.0 31.0 34 10.803789255125876 4.0 4.0 17.0 4.0 31.0 35 10.414482221645471 4.0 4.0 16.0 4.0 30.0 36 10.712950947313782 4.0 4.0 15.0 4.0 33.0 37 10.668310407474696 4.0 4.0 15.0 4.0 33.0 38 10.338178043083312 4.0 4.0 13.0 4.0 32.0 39 10.080716324941603 4.0 4.0 10.0 4.0 32.0 40 9.964962366986764 4.0 4.0 4.0 4.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.09807649395278872 1 2 4.7644363368590135 1 3 -7.239775516579648 1 4 6.373991386620247 1 5 8.924464453072702 1 6 3.9368166198063115 1 7 8.424908510733253 1 8 -2.7146527870754618 1 9 -3.7004987280760453 1 10 0.25470500290088083 1 11 -2.28191748114428 1 12 1.747277636452889 1 13 -7.814940643548898 1 14 1.2571417414200852 1 15 -0.4005567456598378 1 16 2.9991565135895044 1 17 3.8067300397197315 1 18 0.8529611282188618 1 19 -3.566009505957963 1 20 2.0311844512875474 1 21 8.146031374124156 1 22 7.167955326460481 1 23 7.38596197616816 1 24 4.732901548623197 1 25 11.053368367028165 1 26 10.681648591957872 1 27 9.03284241531664 1 28 12.121859240415938 1 29 11.17258892310439 1 30 9.913679876824212 1 31 9.953671843620294 1 32 10.297055607622621 1 33 9.178439773285135 1 34 4.647535368411656 1 35 2.935358370152189 1 36 6.5843977328513414 1 37 5.491856339536753 1 38 6.936307850225374 1 39 9.119511759717943 1 40 7.267213370821617 3 1 -13.098076493952789 3 2 -4.135563663140985 3 3 0.7602244834203518 3 4 -2.0260086133797515 3 5 6.024464453072703 3 6 -1.8631833801936892 3 7 -3.675091489266748 3 8 5.685347212924537 3 9 12.099501271923955 3 10 -14.54529499709912 3 11 -18.28191748114428 3 12 -18.25272236354711 3 13 -19.314940643548898 3 14 -19.442858258579914 3 15 -18.50055674565984 3 16 -15.000843486410496 3 17 -15.99326996028027 3 18 -15.74703887178114 3 19 -12.666009505957962 3 20 -14.268815548712453 3 21 -11.453968625875845 3 22 -11.43204467353952 3 23 -11.714038023831842 3 24 -11.467098451376803 3 25 -10.946631632971835 3 26 -8.91835140804213 3 27 -8.667157584683359 3 28 -8.27814075958406 3 29 -7.427411076895611 3 30 -7.286320123175788 3 31 -6.746328156379706 3 32 -6.3029443923773805 3 33 -5.721560226714864 3 34 -5.352464631588344 3 35 -5.364641629847812 3 36 -5.51560226714866 3 37 -5.408143660463248 3 38 -5.063692149774626 3 39 -4.880488240282057 3 40 -4.732786629178383 4 1 0.9019235060472113 4 2 -19.135563663140985 4 3 -23.23977551657965 4 4 -23.02600861337975 4 5 -17.975535546927297 4 6 -22.86318338019369 4 7 -20.675091489266748 4 8 -18.314652787075463 4 9 -17.900498728076045 4 10 -14.54529499709912 4 11 -18.28191748114428 4 12 -18.25272236354711 4 13 -19.314940643548898 4 14 -19.442858258579914 4 15 -18.50055674565984 4 16 -15.000843486410496 4 17 -15.99326996028027 4 18 -15.74703887178114 4 19 -12.666009505957962 4 20 -14.268815548712453 4 21 -11.453968625875845 4 22 -11.43204467353952 4 23 -11.714038023831842 4 24 -11.467098451376803 4 25 -10.946631632971835 4 26 -8.91835140804213 4 27 -8.667157584683359 4 28 -8.27814075958406 4 29 -7.427411076895611 4 30 -7.286320123175788 4 31 -6.746328156379706 4 32 -6.3029443923773805 4 33 -5.721560226714864 4 34 -5.352464631588344 4 35 -5.364641629847812 4 36 -5.51560226714866 4 37 -5.408143660463248 4 38 -5.063692149774626 4 39 -4.880488240282057 4 40 -4.732786629178383 5 1 -5.598076493952789 5 2 -7.385563663140985 5 3 -8.489775516579648 5 4 -10.526008613379751 5 5 -6.225535546927297 5 6 -9.61318338019369 5 7 -13.675091489266748 5 8 -12.314652787075463 5 9 -12.150498728076045 5 10 -7.54529499709912 5 11 -1.78191748114428 5 12 -6.752722363547111 5 13 -3.5649406435488977 5 14 -4.442858258579914 5 15 -4.000556745659839 5 16 1.9991565135895044 5 17 -0.7432699602802693 5 18 -1.9970388717811396 5 19 4.3339904940420375 5 20 0.4811844512875467 5 21 -0.9539686258758451 5 22 3.3179553264604795 5 23 0.035961976168158216 5 24 4.282901548623197 5 25 1.553368367028165 5 26 6.581648591957871 5 27 4.082842415316641 5 28 5.47185924041594 5 29 2.572588923104389 5 30 7.713679876824212 5 31 -5.246328156379706 5 32 0.44705560762261953 5 33 -5.721560226714864 5 34 -5.352464631588344 5 35 -5.364641629847812 5 36 -5.51560226714866 5 37 -5.408143660463248 5 38 -5.063692149774626 5 39 -4.880488240282057 5 40 -4.732786629178383 6 1 -0.09807649395278872 6 2 -3.802230329807653 6 3 3.7602244834203518 6 4 -0.6926752800464193 6 5 -4.308868880260629 6 6 6.803483286472975 6 7 -4.341758155933416 6 8 -3.6479861204087953 6 9 -6.2338320614093785 6 10 8.788038336234212 6 11 -0.6152508144776121 6 12 -0.5860556968804431 6 13 1.6850593564511023 6 14 7.890475074753418 6 15 1.832776587673493 6 16 5.332489846922837 6 17 2.0067300397197307 6 18 7.25296112821886 6 19 5.000657160708705 6 20 9.397851117954215 6 21 8.546031374124155 6 22 3.9012886597938117 6 23 12.61929530950149 6 24 8.532901548623197 6 25 13.720035033694833 6 26 3.0816485919578707 6 27 -1.667157584683359 6 28 -0.6114740929173941 6 29 4.905922256437721 6 30 1.7136798768242123 6 31 3.9203385102869603 6 32 4.6970556076226195 6 33 7.945106439951804 6 34 5.647535368411656 6 35 1.968691703485522 6 36 15.817731066184672 6 37 13.925189672870085 6 38 14.936307850225374 6 39 15.119511759717943 6 40 10.933880037488285 7 1 0.3304949346186419 7 2 5.435864908287588 7 3 2.903081626277494 7 4 -0.026008613379751466 7 5 2.7387501673584183 7 6 -0.5774690944794045 7 7 0.18205136787610954 7 8 -0.17179564421832083 7 9 1.956644129066813 7 10 6.311847860043738 7 11 8.71808251885572 7 12 7.461563350738604 7 13 11.256487927879672 7 14 6.985713169991513 7 15 8.642300397197303 7 16 12.28487079930379 7 17 11.292444325434015 7 18 5.967246842504576 7 19 14.47684763689918 7 20 13.159755879858974 7 21 10.974602802695582 7 22 17.282241040746193 7 23 16.571676261882445 7 24 14.961472977194624 7 25 15.624796938456738 7 26 9.510220020529298 7 27 5.761413843888068 7 28 14.864716383273082 7 29 14.858303208818674 7 30 13.427965591109928 7 31 11.253671843620294 7 32 5.839912750479762 7 33 7.992725487570851 7 34 4.504678225554514 7 35 0.7782155130093305 7 36 -0.6584594100058023 7 37 0.5918563395367524 7 38 1.3648792787968027 7 39 -2.737631097424914 7 40 -2.732786629178383 9 1 -3.0980764939527887 9 2 -14.135563663140985 9 3 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2.933555362163613 10 6 5.6822711652608575 10 7 4.688544874369615 10 8 6.4126199401972634 10 9 5.0085921810148655 10 10 9.36379591199179 10 11 5.62717342794663 10 12 6.201823090998342 10 13 6.775968447360192 10 14 6.829869014147359 10 15 4.590352345249251 10 16 8.180974695407688 10 17 10.91582094881064 10 18 10.434779310037044 10 19 13.6976268576784 10 20 11.00391172401482 10 21 6.636940465033245 10 22 14.56795532646048 10 23 15.740507430713611 10 24 8.078356094077744 10 25 12.689732003391802 10 26 8.899830410139687 10 27 9.332842415316641 10 28 4.4491319676886665 10 29 7.936225286740752 10 30 8.804588967733302 10 31 6.7082172981657475 10 32 6.424328334895346 10 33 7.4602579551033195 10 34 10.10208082295711 10 35 8.635358370152188 10 36 5.302579551033158 10 37 8.22821997590039 10 38 3.845398759316282 10 39 7.66496630517249 10 40 5.63084973445798 12 1 3.5269235060472113 12 2 3.114436336859015 12 3 6.135224483420352 12 4 8.598991386620249 12 5 8.649464453072703 12 6 7.136816619806311 12 7 7.324908510733252 12 8 10.935347212924537 12 9 6.349501271923955 12 10 10.82970500290088 12 11 14.59308251885572 12 12 11.497277636452889 12 13 11.435059356451102 12 14 12.057141741420086 12 15 12.12444325434016 12 16 12.499156513589504 12 17 13.38173003971973 12 18 13.75296112821886 12 19 17.333990494042038 12 20 18.481184451287547 12 21 12.296031374124155 12 22 6.4429553264604795 12 23 8.660961976168158 12 24 9.282901548623197 12 25 10.553368367028165 12 26 5.206648591957871 12 27 5.082842415316641 12 28 9.84685924041594 12 29 6.072588923104389 12 30 5.713679876824212 12 31 3.6286718436202943 12 32 1.9470556076226195 12 33 1.778439773285136 12 34 3.3975353684116563 12 35 3.135358370152188 12 36 0.48439773285134 12 37 4.841856339536752 12 38 1.8113078502253739 12 39 4.244511759717943 12 40 2.642213370821617 13 1 2.9019235060472113 13 2 8.614436336859015 13 3 -2.9897755165796482 13 4 6.2239913866202485 13 5 6.524464453072703 13 6 -0.8631833801936892 13 7 -4.175091489266748 13 8 0.6853472129245368 13 9 5.099501271923955 13 10 4.45470500290088 13 11 4.71808251885572 13 12 4.747277636452889 13 13 9.935059356451102 13 14 3.307141741420086 13 15 1.7494432543401608 13 16 12.749156513589504 13 17 11.50673003971973 13 18 7.75296112821886 13 19 9.583990494042038 13 20 10.731184451287547 13 21 5.546031374124155 13 22 8.06795532646048 13 23 10.285961976168158 13 24 8.282901548623197 13 25 5.803368367028165 13 26 5.331648591957871 13 27 7.582842415316641 13 28 3.47185924041594 13 29 9.822588923104389 13 30 14.713679876824212 13 31 11.503671843620294 13 32 7.1970556076226195 13 33 11.778439773285136 13 34 9.897535368411656 13 35 7.385358370152188 13 36 14.73439773285134 13 37 14.091856339536752 13 38 14.436307850225374 13 39 12.619511759717943 13 40 11.017213370821617 16 1 4.901923506047211 16 2 9.864436336859015 16 3 4.093557816753684 16 4 10.307324719953584 16 5 -1.3088688802606292 16 6 7.803483286472975 16 7 0.658241844066584 16 8 3.3520138795912047 16 9 0.7661679385906233 16 10 7.121371669567548 16 11 5.384749185522388 16 12 -2.586055696880443 16 13 10.351726023117767 16 14 8.557141741420086 16 15 1.4994432543401608 16 16 -8.434864104955864E-4 16 17 7.340063373053063 16 18 7.919627794885528 16 19 9.66732382737537 16 20 8.397851117954215 16 21 3.2126980407908228 16 22 5.5679553264604795 16 23 13.61929530950149 16 24 13.86623488195653 16 25 7.386701700361497 16 26 8.08164859195787 16 27 7.332842415316641 16 28 10.055192573749272 16 29 5.239255589771057 16 30 8.713679876824212 16 31 3.587005176953628 16 32 4.0303889409559535 16 33 -2.054893560048197 16 34 4.98086870174499 16 35 4.968691703485522 16 36 4.817731066184674 16 37 -1.7414769937965806 16 38 5.269641183558708 16 39 5.452845093051277 16 40 5.600546704154951 17 1 1.3019235060472099 17 2 3.1644363368590156 17 3 1.6602244834203503 17 4 2.673991386620248 17 5 -0.17553554692729634 17 6 5.536816619806309 17 7 8.024908510733255 17 8 6.285347212924538 17 9 4.199501271923957 17 10 5.95470500290088 17 11 6.618082518855719 17 12 7.747277636452889 17 13 5.785059356451104 17 14 8.557141741420086 17 15 9.89944325434016 17 16 8.499156513589504 17 17 12.40673003971973 17 18 8.652961128218859 17 19 14.533990494042037 17 20 11.631184451287545 17 21 14.046031374124155 17 22 14.967955326460478 17 23 13.585961976168159 17 24 11.832901548623198 17 25 5.6533683670281665 17 26 -1.4183514080421293 17 27 1.0328424153166402 17 28 -0.7781407595840601 17 29 1.3725889231043897 17 30 1.1136798768242127 17 31 -0.1463281563797061 17 32 -2.702944392377381 17 33 -2.921560226714864 17 34 -2.552464631588344 17 35 -2.1646416298478117 17 36 -1.3156022671486607 17 37 -1.2081436604632483 17 38 -0.9636921497746265 17 39 0.5195117597179433 17 40 -0.9327866291783833 18 1 -0.9980764939527873 18 2 -1.1355636631409851 18 3 -2.539775516579649 18 4 -0.8260086133797522 18 5 1.524464453072703 18 6 5.036816619806309 18 7 5.7249085107332505 18 8 5.585347212924535 18 9 4.899501271923956 18 10 6.254705002900881 18 11 6.21808251885572 18 12 5.04727763645289 18 13 4.085059356451101 18 14 7.4571417414200845 18 15 0.4994432543401608 18 16 -1.8008434864104963 18 17 0.30673003971973145 18 18 4.552961128218861 18 19 2.533990494042037 18 20 -1.9688155487124526 18 21 10.646031374124156 18 22 7.767955326460479 18 23 8.485961976168158 18 24 7.532901548623197 18 25 4.953368367028164 18 26 0.5816485919578707 18 27 7.932842415316642 18 28 7.021859240415941 18 29 6.972588923104388 18 30 7.113679876824211 18 31 5.353671843620296 18 32 4.09705560762262 18 33 4.978439773285135 18 34 2.747535368411656 18 35 3.935358370152189 18 36 5.48439773285134 18 37 4.591856339536752 18 38 4.336307850225374 18 39 4.719511759717943 18 40 4.8672133708216165 19 1 0.6519235060472113 19 2 -1.6355636631409851 19 3 -2.7397755165796482 19 4 4.2239913866202485 19 5 -0.47553554692729705 19 6 5.636816619806311 19 7 3.824908510733252 19 8 7.435347212924537 19 9 5.599501271923955 19 10 10.20470500290088 19 11 6.46808251885572 19 12 6.997277636452889 19 13 8.685059356451102 19 14 8.307141741420086 19 15 12.49944325434016 19 16 8.499156513589504 19 17 12.25673003971973 19 18 11.50296112821886 19 19 15.833990494042038 19 20 15.731184451287547 19 21 3.296031374124155 19 22 4.8179553264604795 19 23 6.785961976168158 19 24 7.282901548623197 19 25 8.553368367028165 19 26 5.331648591957871 19 27 10.082842415316641 19 28 9.47185924041594 19 29 1.572588923104389 19 30 2.7136798768242123 19 31 1.2536718436202943 19 32 4.6970556076226195 19 33 2.778439773285136 19 34 7.147535368411656 19 35 6.135358370152188 19 36 2.23439773285134 19 37 2.0918563395367524 19 38 4.186307850225374 19 39 4.619511759717943 19 40 9.267213370821617 20 1 -1.0980764939527887 20 2 2.864436336859015 20 3 -0.23977551657964824 20 4 7.307324719953584 20 5 1.024464453072703 20 6 -1.529850046860357 20 7 0.9915751773999197 20 8 -1.6479861204087953 20 9 0.43283460525728756 20 10 3.45470500290088 20 11 13.051415852189056 20 12 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-14.268815548712453 120 21 -11.453968625875845 120 22 -11.43204467353952 120 23 -11.714038023831842 120 24 -11.467098451376803 120 25 -10.946631632971835 120 26 -8.91835140804213 120 27 -8.667157584683359 120 28 -8.27814075958406 120 29 -7.427411076895611 120 30 -7.286320123175788 120 31 -6.746328156379706 120 32 -6.3029443923773805 120 33 -5.721560226714864 120 34 -5.352464631588344 120 35 -5.364641629847812 120 36 -5.51560226714866 120 37 -5.408143660463248 120 38 -5.063692149774626 120 39 -4.880488240282057 120 40 -4.732786629178383 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 244.0 5 32.0 6 86.0 7 128.0 8 82.0 9 50.0 10 72.0 11 94.0 12 111.0 13 115.0 14 150.0 15 150.0 16 166.0 17 163.0 18 168.0 19 202.0 20 201.0 21 199.0 22 213.0 23 175.0 24 193.0 25 180.0 26 147.0 27 145.0 28 129.0 29 117.0 30 64.0 31 31.0 32 24.0 33 14.0 34 7.0 35 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 98.51832596828697 0.6498570314530803 0.5458799064205875 0.28593709383935534 8 96.44155844155844 1.6883116883116882 0.7272727272727273 1.1428571428571428 9 94.12987012987013 3.272727272727273 1.7662337662337664 0.8311688311688312 10 33.24682065922658 42.51232805606021 11.783026213340255 12.457825071372957 11 26.005709836491047 33.68803529717104 21.723332468206593 18.582922398131327 12 37.29561380742279 21.411886841422266 21.04853361017389 20.243965740981054 13 23.903451855696858 17.93407734233065 23.150791590968076 35.011679211004406 14 26.732416298987804 27.043861925772127 24.396574098105372 21.8271476771347 15 29.353750324422528 22.60576174409551 22.631715546327538 25.40877238515443 16 25.590449000778616 27.822475992732937 25.434726187386453 21.152348819102 17 33.40254347261874 21.282117830262134 24.137036075785105 21.178302621334026 18 30.755255644951983 23.04697638203997 21.697378665974565 24.50038930703348 19 26.307572209211553 17.720530835284933 20.76502732240437 35.20686963309914 20 24.604204515961587 29.84687256683104 21.515702050350377 24.033220866856993 21 22.94316117311186 22.7874383597197 31.637684920840904 22.631715546327538 22 31.014793667272254 19.776797300804567 19.776797300804567 29.431611731118608 23 25.824033220866855 27.770568388268877 23.592006228912535 22.813392161951725 24 30.70334804048793 22.579807941863482 25.40877238515443 21.308071632494162 25 26.668397818748378 27.73305634900026 23.682160477797975 21.91638535445339 26 24.843423799582464 20.224425887265134 31.758872651356995 23.173277661795407 27 31.178608515057114 16.043613707165107 27.466251298027 25.31152647975078 28 28.263690630677395 20.814949390085648 28.082014015053204 22.839345964183753 29 26.26105044201768 18.902756110244407 30.83723348933957 23.998959958398338 30 18.460738429537184 19.81279251170047 34.06136245449818 27.66510660426417 31 17.711313394018205 28.608582574772434 32.925877763329 20.754226267880362 32 17.39356178608515 22.71547248182762 34.86500519210799 25.02596053997923 33 14.949390085647549 25.33091097845834 35.4788476511809 24.240851284713212 34 14.637944458863222 24.993511549441994 38.307812094471835 22.060731897222944 35 12.490262269540379 25.16229550766035 41.67748636717736 20.669955855621915 36 12.883116883116882 26.64935064935065 38.5974025974026 21.87012987012987 37 13.843351548269581 24.512099921935988 42.362737444704656 19.28181108508977 38 16.091357383856735 25.304957176226317 38.95665715027252 19.64702828964443 39 14.749415736172422 25.889379381978706 38.56141262009868 20.799792261750195 40 14.041007007526602 26.68050869452375 41.26654554892292 18.011938749026733 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 6.5 17 12.0 18 12.0 19 13.5 20 15.0 21 16.0 22 17.0 23 17.0 24 23.0 25 29.0 26 39.0 27 49.0 28 49.0 29 60.5 30 72.0 31 72.0 32 72.0 33 72.0 34 79.5 35 87.0 36 112.0 37 137.0 38 137.0 39 143.5 40 150.0 41 172.0 42 194.0 43 194.0 44 200.5 45 207.0 46 245.5 47 284.0 48 284.0 49 307.0 50 330.0 51 310.0 52 290.0 53 290.0 54 279.0 55 268.0 56 271.0 57 274.0 58 274.0 59 271.0 60 268.0 61 246.0 62 224.0 63 224.0 64 239.0 65 254.0 66 220.0 67 186.0 68 186.0 69 176.5 70 167.0 71 151.5 72 136.0 73 136.0 74 98.0 75 60.0 76 48.0 77 36.0 78 36.0 79 28.0 80 20.0 81 14.5 82 9.0 83 9.0 84 6.0 85 3.0 86 2.5 87 2.0 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.15572281339216196 8 0.07786140669608098 9 0.07786140669608098 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.25953802232026996 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.05190760446405398 26 0.5450298468725668 27 0.02595380223202699 28 0.0 29 0.18167661562418894 30 0.18167661562418894 31 0.20763041785621592 32 0.02595380223202699 33 0.0 34 0.0 35 0.05190760446405398 36 0.07786140669608098 37 0.25953802232026996 38 0.0 39 0.05190760446405398 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 3853.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.01842719958474 #Duplication Level Percentage of deduplicated Percentage of total 1 95.81473214285714 89.12535686478068 2 3.013392857142857 5.606021282117831 3 0.5580357142857143 1.5572281339216196 4 0.1953125 0.7267064624967557 5 0.13950892857142858 0.6488450558006749 6 0.055803571428571425 0.3114456267843239 7 0.055803571428571425 0.3633532312483779 8 0.0 0.0 9 0.08370535714285714 0.7007526602647288 >10 0.08370535714285714 0.9602906825849987 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 16 0.41526083571243183 No Hit GAGGAGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAA 11 0.28549182455229694 No Hit GAGGAGGGGACACGCAGGGATGAGATGGGCAAAAAAAAAA 10 0.25953802232026996 No Hit GAGGAGGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT 9 0.23358422008824295 No Hit GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 9 0.23358422008824295 No Hit GAGGAGGGGACACGCAGGGATGAGATGGGGAAAAAAAAAA 9 0.23358422008824295 No Hit GAGGAGGGGGATCCAGTGACAGATACCATTAGATTCAAGA 7 0.18167661562418894 No Hit GAGGAGGGGGAGTGCAGTGCTGAGGAGGGGACACGCAGGG 7 0.18167661562418894 No Hit GAGGAGGGGGTGCAGTGCTGAGGAGGGGACACGCAGGGAT 6 0.15572281339216196 No Hit GAGGAGGGGGTGCAGTGCTGAGGAGGGGACACGCCGGGAT 6 0.15572281339216196 No Hit GAGGAGGGGACACGCAGGGATGAGATGGCGAAAAAAAAAA 5 0.12976901116013498 No Hit GAGGAGGGGCCACCACACCTGGCTTATAGTGAGTCCTTTG 5 0.12976901116013498 No Hit GAGGAGGGGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTT 5 0.12976901116013498 No Hit GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 5 0.12976901116013498 No Hit GAGGAGGGGGAGTGCAGTGCTGAGGAGGGGACACGCCGGG 5 0.12976901116013498 No Hit GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCCACTTTT 4 0.10381520892810796 No Hit GAGGAGGGGAGCCTTTTAACCTGGAGACTTGTCGCCTTAT 4 0.10381520892810796 No Hit GAGGAGGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT 4 0.10381520892810796 No Hit GAGGAGGGGGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAA 4 0.10381520892810796 No Hit GAGGAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.10381520892810796 No Hit GAGGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG 4 0.10381520892810796 No Hit GAGGAGGGGGACTAAGTTGTCCCGAGAACTGACTTTCTCC 4 0.10381520892810796 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.025953802232026993 0.0 0.0 0.0 0.0 11 0.051907604464053986 0.0 0.0 0.0 0.0 12 0.051907604464053986 0.0 0.0 0.0 0.0 13 0.051907604464053986 0.0 0.0 0.0 0.0 14 0.051907604464053986 0.0 0.0 0.0 0.0 15 0.051907604464053986 0.0 0.0 0.0 0.0 16 0.051907604464053986 0.0 0.0 0.0 0.0 17 0.051907604464053986 0.0 0.0 0.0 0.0 18 0.07786140669608098 0.0 0.0 0.0 0.0 19 0.07786140669608098 0.0 0.0 0.0 0.0 20 0.07786140669608098 0.0 0.0 0.0 0.0 21 0.07786140669608098 0.0 0.0 0.0 0.0 22 0.07786140669608098 0.0 0.0 0.0 0.0 23 0.07786140669608098 0.0 0.0 0.0 0.0 24 0.07786140669608098 0.0 0.0 0.0 0.0 25 0.10381520892810797 0.0 0.0 0.0 0.0 26 0.10381520892810797 0.0 0.0 0.0 0.0 27 0.10381520892810797 0.0 0.0 0.0 0.0 28 0.10381520892810797 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGTG 20 0.0025249545 33.818184 5 AGGGGGA 30 1.1797429E-5 33.818184 5 AGGGGCA 35 7.9716665E-7 33.818184 5 AGGGGAT 20 0.0025249545 33.818184 5 AGGGGAG 35 7.9716665E-7 33.818184 5 AGGGGAA 50 2.3283064E-10 33.818184 5 GGGGAAG 30 1.1797429E-5 33.818184 6 GAGGGGC 70 0.0 31.402597 4 GAGGGGA 130 0.0 31.216782 4 GGAGGGG 380 0.0 30.70335 3 AGGAGGG 395 0.0 30.393557 2 GAGGAGG 420 0.0 30.194805 1 GAGGGGG 125 0.0 29.76 4 AGGGGGT 40 2.265957E-6 29.59091 5 AGGGGGG 40 2.265957E-6 29.59091 5 GGGGGAG 30 5.052797E-4 28.18182 6 GAGGGGT 65 3.8835424E-9 26.013985 4 GGGGGTG 35 0.001244725 24.155844 6 TTTTTTT 545 5.1901134E-6 5.584654 34 >>END_MODULE