FastQCFastQC Report
Fri 17 Jun 2016
SRR1525895_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525895_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5721
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1412.464604090194022No Hit
GAGGAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT410.7166579269358504No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC390.6816990036706869No Hit
GAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.3146303093864709No Hit
GAGGAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG180.3146303093864709No Hit
GAGGAGGGGATTGGGCGGATTGAACAAAAACTCATCTCAG160.27967138612130743No Hit
GAGGAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA120.2097535395909806No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG120.2097535395909806No Hit
GAGGAGGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT110.19227407795839888No Hit
GAGGAGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT110.19227407795839888No Hit
GAGGAGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT100.17479461632581716No Hit
GAGGAGGGGGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA100.17479461632581716No Hit
GAGGAGGGGCGGATTGAAAAAAAACTCATCTCAGAAGAGG100.17479461632581716No Hit
GAGGAGGGGTACATGGTTGATCCTGCCAGTAGCATATGCT90.15731515469323545No Hit
GAGGAGGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT80.13983569306065372No Hit
GAGGAGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG80.13983569306065372No Hit
GAGGAGGGGCGGATTGAACAAAAACTCATCTCAGAAGAGG80.13983569306065372No Hit
GAGGAGGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGC80.13983569306065372No Hit
GAGGAGGGGGGGGAGGTAGTGACGAAAAATAACAATACAG80.13983569306065372No Hit
GAGGAGGGGATGCCACAAATAGATACATCAACATGATTTA70.12235623142807202No Hit
GAGGAGGGGATGCCACAACTAGATACATCAACATGATTTA70.12235623142807202No Hit
GAGGAGGGGTACCTGGTTGATACTGCCAGTAGCATATGCT70.12235623142807202No Hit
GAGGAGGGGGATCCTGCCAGTAGCATATGCTTGTCTCAAA60.1048767697954903No Hit
GAGGAGGGGGGCGGATTGAACAAAAACTCATCTCAGAAGA60.1048767697954903No Hit
GAGGAGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT60.1048767697954903No Hit
GAGGAGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT60.1048767697954903No Hit
GAGGAGGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTA60.1048767697954903No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG60.1048767697954903No Hit
GAGGAGGTGGCTTAGAGCTGTCTTAGTCACTAAAGCATGG60.1048767697954903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1150.034.4821475
AGGGGTC405.2174073E-834.4821435
AGGGGTA251.641156E-434.4821435
AGGGGGT750.034.4821435
AGGGGGA1000.034.4821435
AGGGGCA200.002388847834.4821435
AGGGGAT251.641156E-434.4821435
AGGGGAA200.002388847834.4821435
GGGGGTG301.124279E-534.4821436
GAGGGGT850.034.4821434
GAGGGGC357.675317E-734.4821434
GAGGGGA700.034.4821434
GGAGGGG5100.034.1440853
GAGGGGG3200.033.943364
ATCCTCT200.002609597333.87719316
TCCTCTT200.002609597333.87719317
CTCTTCT251.8254234E-433.87719319
CCTCTTC200.002609597333.87719318
TCTTCTG200.002609597333.87719320
AGATCCT200.002609597333.87719314
Produced by FastQC (version 0.11.5)