SRR1525894_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1525894_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17362
Sequences flagged as poor quality	0
Sequence length	56
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTCAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	152	0.8754751756710055	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	94	0.5414122796912798	No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	86	0.49533463886649004	No Hit
GCTCAAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	82	0.47229581845409513	No Hit
GCTCAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	78	0.4492569980417003	No Hit
GCTCAAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	54	0.3110240755673309	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	38	0.21886879391775138	No Hit
GCTCAAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	34	0.1958299735053565	No Hit
GCTCAAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	31	0.17855085819606034	No Hit
GCTCAAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	30	0.17279115309296164	No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	30	0.17279115309296164	No Hit
GCTCAAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	30	0.17279115309296164	No Hit
GCTCAAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	29	0.1670314479898629	No Hit
GCTCAAGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	29	0.1670314479898629	No Hit
GCTCAAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	29	0.1670314479898629	No Hit
GCTCAAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	28	0.16127174288676419	No Hit
GCTCAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	28	0.16127174288676419	No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTG	27	0.15551203778366546	No Hit
GCTCAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	26	0.14975233268056676	No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	25	0.14399262757746803	No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACAGATTGTTCTTCC	25	0.14399262757746803	No Hit
GCTCAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	24	0.1382329224743693	No Hit
GCTCAAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC	22	0.12671351226817187	No Hit
GCTCAAGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG	21	0.12095380716507313	No Hit
GCTCAAGGGGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA	21	0.12095380716507313	No Hit
GCTCAAGGGGAGGTCCGGGCCTTTGACCCAGACACAGCAGCCGTGCAGCCCTACCA	19	0.10943439695887569	No Hit
GCTCAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA	19	0.10943439695887569	No Hit
GCTCAAGGGGTTGCGAGATCGTGGGTTTGAGTCCCACCTCGCACCTTGTTGCGAGA	18	0.10367469185577699	No Hit
GCTCAAGGGGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACAT	18	0.10367469185577699	No Hit
GCTCAAGGGCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG	18	0.10367469185577699	No Hit
GCTCAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	18	0.10367469185577699	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCTC	15	0.008077702	50.331394	40
GCTTGTC	15	0.008077702	50.331394	38
CTTGTCT	15	0.008077702	50.331394	39
GCAAAAG	15	0.008171324	50.185505	35
AAGATTA	25	1.9930976E-5	50.040466	48
AGATTAA	25	1.9930976E-5	50.040466	49
AAGCAGT	25	1.9930976E-5	50.040466	10
ACCTGGT	20	4.0535734E-4	50.040462	11
ATATGCT	20	4.0535734E-4	50.040462	34
TCTCAAA	20	4.0535734E-4	50.040462	43
AAAGATT	20	4.0535734E-4	50.040462	47
CCTGGTT	20	4.0535734E-4	50.040462	12
GTACCTG	30	9.832438E-7	50.04046	9
GCGGCTT	15	0.008265751	50.04046	50
GAAGCAG	30	9.832438E-7	50.04046	9
AGGGGTC	45	1.2369128E-10	49.89626	6
AGGGGCT	90	0.0	49.89626	6
AGGGGCG	25	2.0276657E-5	49.896255	6
AGGGGAT	50	5.456968E-12	49.896255	6
CCAGTAG	25	2.0276657E-5	49.896255	26

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content