##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525894_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17362 Sequences flagged as poor quality 0 Sequence length 56 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.60171639212072 38.0 35.0 39.0 29.0 40.0 2 35.27709941251008 38.0 35.0 39.0 27.0 40.0 3 34.99804170026495 38.0 34.0 39.0 26.0 40.0 4 35.096993433936184 38.0 35.0 39.0 25.0 40.0 5 32.03657412740468 38.0 28.0 39.0 12.0 40.0 6 31.189955074300197 38.0 23.0 39.0 15.0 40.0 7 34.3559497753715 38.0 33.0 39.0 26.0 40.0 8 34.46002764658449 38.0 33.0 39.0 26.0 40.0 9 34.51578159198249 38.0 33.0 39.0 25.0 40.0 10 34.04947586683562 38.0 33.0 39.0 23.0 40.0 11 34.06594862343048 38.0 33.0 39.0 23.0 40.0 12 32.80918096993434 38.0 30.0 39.0 18.0 40.0 13 32.346849441308606 37.0 29.0 39.0 17.0 40.0 14 32.95674461467573 38.0 31.0 39.0 19.0 40.0 15 32.03075682525055 37.0 29.0 39.0 15.0 40.0 16 32.774277157009564 37.0 30.0 39.0 19.0 40.0 17 31.92132242829167 36.0 29.0 39.0 15.0 40.0 18 31.263621702568827 36.0 27.0 39.0 14.0 40.0 19 32.3412625273586 36.0 30.0 39.0 17.0 40.0 20 32.98957493376339 37.0 31.0 39.0 21.0 40.0 21 32.99625619168299 38.0 31.0 39.0 19.0 40.0 22 32.32450178550858 37.0 30.0 39.0 16.0 40.0 23 32.96682409860615 38.0 31.0 39.0 19.0 40.0 24 32.39148715585762 37.0 30.0 39.0 17.0 40.0 25 32.78666052298122 37.0 31.0 39.0 19.0 40.0 26 33.117785969358366 38.0 32.0 39.0 18.0 40.0 27 33.42765810390508 38.0 33.0 39.0 20.0 40.0 28 33.45870291441078 38.0 33.0 39.0 22.0 40.0 29 32.98830779864071 38.0 32.0 39.0 18.0 40.0 30 32.77110931920286 37.0 31.0 39.0 18.0 40.0 31 33.5711899550743 38.0 33.0 39.0 21.0 40.0 32 33.463598663748414 38.0 33.0 39.0 20.0 40.0 33 33.234938371155394 38.0 33.0 39.0 19.0 40.0 34 33.09313443151711 37.0 33.0 39.0 18.0 40.0 35 33.000403179357214 37.0 32.0 39.0 18.0 40.0 36 32.65827669623315 37.0 32.0 38.0 17.0 40.0 37 32.416714664209195 36.0 31.0 38.0 16.0 40.0 38 31.666455477479552 36.0 30.0 38.0 10.0 40.0 39 30.63080290289137 35.0 28.0 38.0 4.0 40.0 40 28.71639212072342 34.0 23.0 38.0 4.0 39.0 41 27.005932496256193 32.0 18.0 38.0 4.0 39.0 42 27.602465153784127 33.0 21.0 38.0 4.0 39.0 43 27.744326690473446 33.0 22.0 38.0 4.0 39.0 44 26.82663287639673 32.0 18.0 38.0 4.0 39.0 45 27.664957954152747 33.0 23.0 38.0 4.0 39.0 46 25.59618707522175 30.0 17.0 36.0 4.0 39.0 47 24.601889183273816 29.0 11.0 36.0 4.0 39.0 48 25.279806473908536 30.0 16.0 36.0 4.0 39.0 49 24.168816956571824 29.0 10.0 36.0 4.0 39.0 50 24.888204123948853 30.0 13.0 36.0 4.0 39.0 51 24.991475636447415 30.0 6.0 36.0 4.0 39.0 52 24.880774104365855 30.0 4.0 36.0 4.0 39.0 53 24.735975118073956 30.0 4.0 36.0 4.0 39.0 54 24.29345697500288 30.0 4.0 36.0 4.0 39.0 55 23.985370349038128 30.0 4.0 36.0 4.0 39.0 56 23.41314364704527 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 5.10891150719916 1 2 5.165079026899505 1 3 6.139478082902457 1 4 5.448791694631318 1 5 9.550060739285879 1 6 10.520409972264982 1 7 5.9200815398522835 1 8 6.3019407559716 1 9 6.403002518128069 1 10 6.624254961901734 1 11 3.3451458129684504 1 12 -5.615184171805204 1 13 -15.91928702817435 1 14 -5.7039589055615245 1 15 3.0255552034361877 1 16 -0.07685443347957488 1 17 -3.7997137578358036 1 18 -5.970269277709537 1 19 0.22941094260756145 1 20 0.3656431485385063 1 21 7.177019690923057 1 22 7.44624185387314 1 23 6.787120027537416 1 24 7.0544158812873405 1 25 5.397602826186908 1 26 5.906472619659972 1 27 5.38697327347008 1 28 6.667965572170242 1 29 6.802521347551288 1 30 6.055440883489883 1 31 6.123042107017422 1 32 5.433112294137921 1 33 6.4257184593787 1 34 5.346780014440533 1 35 4.136112010435113 1 36 2.222890775809738 1 37 3.410130652541344 1 38 3.204654746517914 1 39 3.231648020786338 1 40 -11.533994357941115 1 41 -0.9532868577935538 1 42 -3.9896785836442454 1 43 -9.14302030697279 1 44 -15.968426170340818 1 45 -2.9729920470309636 1 46 -5.376424348100102 1 47 -11.706066550106046 1 48 -5.3661762986745 1 49 -13.295712531183765 1 50 -3.1911445943173007 1 51 3.793085047404798 1 52 4.01150709751667 1 53 3.780930422705932 1 54 4.626174136390251 1 55 4.056708602835329 1 56 9.633337956654433 2 1 5.76442990184799 2 2 5.753707789441307 2 3 6.48730416985898 2 4 6.644443868544364 2 5 10.389525622229026 2 6 10.9986708418302 2 7 7.002021339183386 2 8 7.213311993429798 2 9 7.4448085381949625 2 10 6.791479042169293 2 11 6.821734441730992 2 12 3.5353175004355997 2 13 5.739575848079827 2 14 6.985004305140819 2 15 8.426892996078326 2 16 5.908095399296343 2 17 8.3574768776157 2 18 6.210332728979424 2 19 7.518708601470436 2 20 6.885710038170615 2 21 8.310798955137106 2 22 9.538215098020295 2 23 8.104845780045782 2 24 8.758429259213756 2 25 8.410980752608314 2 26 8.125536164810477 2 27 7.8568729390219225 2 28 8.285022428357532 2 29 8.777437735511153 2 30 8.165808776466474 2 31 6.726721036783314 2 32 6.578597243970698 2 33 5.644782004529205 2 34 5.04243218835358 2 35 4.438787595719397 2 36 6.196134922966927 2 37 6.6726724585614114 2 38 5.890273475614908 2 39 6.495862067609078 2 40 4.173363501590657 2 41 8.180492406420491 2 42 7.727712720703579 2 43 7.025876014097445 2 44 -3.552038210474599 2 45 7.772827350962345 2 46 5.682104079993543 2 47 1.9996190686230513 2 48 5.857903968884031 2 49 -1.5699600228225599 2 50 6.241965773575675 2 51 9.80311849222085 2 52 8.896122482132053 2 53 9.397987278893225 2 54 9.923832999266505 2 55 9.795839037617938 2 56 10.71360551518286 3 1 5.885309022727114 3 2 5.707004492738008 3 3 6.915875598430411 3 4 6.573015297115795 3 5 10.337327820031224 3 6 11.355813698973055 3 7 6.892131229293277 3 8 6.724301004418805 3 9 6.920083263469685 3 10 7.478292228982475 3 11 6.118437738434292 3 12 7.582020797138899 3 13 8.302762661266645 3 14 7.493246063382578 3 15 3.7181017872871216 3 16 7.614139355340299 3 17 8.467366987505809 3 18 9.023519542166241 3 19 7.903323986085823 3 20 7.325270477731053 3 21 7.9316780760162295 3 22 8.61239092219612 3 23 7.981219406419402 3 24 8.774912775697274 3 25 8.287354378981934 3 26 7.3288328681071775 3 27 6.947532279681258 3 28 6.980077373412481 3 29 6.942272900346314 3 30 8.297676908334605 3 31 6.616830926893204 3 32 6.229696145069596 3 33 5.919507279254482 3 34 6.113860759782149 3 35 6.235490892422696 3 36 6.907673384505387 3 37 7.3402548761438275 3 38 6.491921827263258 3 39 8.259598331345341 3 40 9.560726138953296 3 41 12.479942955871039 3 42 11.441998434989294 3 43 10.95170018992162 3 44 12.140269481833094 3 45 11.086014164149162 3 46 12.904631552521014 3 47 14.362256431260413 3 48 14.028233639213699 3 49 14.017952065089524 3 50 14.038669070278974 3 51 13.987184426286781 3 52 14.368649954659524 3 53 13.771613652519601 3 54 13.860646186079691 3 55 14.367267609046507 3 56 14.93613298771033 4 1 4.556737594155685 4 2 4.93319496892849 4 3 6.320637503192316 4 4 5.611110535211033 4 5 9.492089724793129 4 6 10.598670841830202 4 7 6.096893134055179 4 8 5.7338248139426184 4 9 6.26532135870778 4 10 6.240196990887242 4 11 5.608913928910479 4 12 8.222496987615084 4 13 6.857524566028548 4 14 7.6003889205254325 4 15 6.203816073001409 4 16 6.826044117245061 4 17 6.929271749410571 4 18 8.361614780261476 4 19 7.236657319419159 4 20 5.4395561920167665 4 21 7.169773314111463 4 22 6.276676636481838 4 23 5.7407432159432155 4 24 6.989198489982989 4 25 7.446878188505746 4 26 6.738356677630989 4 27 5.792770374919353 4 28 5.382458325793433 4 29 6.218463376536793 4 30 5.411962622620319 4 31 4.321592831655106 4 32 4.260648526021981 4 33 4.324269184016387 4 34 4.04243218835358 4 35 4.492633749565549 4 36 5.057673384505385 4 37 5.652159638048591 4 38 6.913350398691829 4 39 7.121503093250102 4 40 2.0964404246675805 4 41 10.472800098728186 4 42 6.994379387370245 4 43 7.720747808969239 4 44 9.240269481833096 4 45 8.757442735577733 4 46 10.330822028711491 4 47 10.543208812212796 4 48 9.409186020166079 4 49 7.360809207946669 4 50 7.795811927421827 4 51 8.01337490247726 4 52 7.311507097516671 4 53 6.86208984299579 4 54 8.239217614651121 4 55 7.095839037617939 4 56 8.195656797234143 5 1 5.356737594155682 5 2 5.166528302261824 5 3 6.1539708365256445 5 4 6.077777201877694 5 5 10.258756391459794 5 6 10.9986708418302 5 7 6.163559800721849 5 8 6.500491480609284 5 9 7.098654692041116 5 10 7.173530324220572 5 11 7.342247262243813 5 12 8.122496987615083 5 13 8.45752456602855 5 14 8.200388920525434 5 15 9.503816073001406 5 16 8.192710783911728 5 17 9.029271749410572 5 18 10.228281446928143 5 19 8.036657319419156 5 20 7.972889525350098 5 21 8.569773314111469 5 22 8.876676636481832 5 23 7.574076549276551 5 24 7.7891984899829865 5 25 8.446878188505746 5 26 7.905023344297653 5 27 5.359437041586023 5 28 7.015791659126766 5 29 6.951796709870127 5 30 7.545295955953652 5 31 6.321592831655106 5 32 5.760648526021981 5 33 6.024269184016383 5 34 5.842432188353577 5 35 5.892633749565547 5 36 7.491006717838722 5 37 6.852159638048594 5 38 7.713350398691826 5 39 8.721503093250103 5 40 9.729773758000913 5 41 12.106133432061519 5 42 11.961046054036913 5 43 11.82074780896924 5 44 12.940269481833091 5 45 12.090776068911062 5 46 14.030822028711494 5 47 15.10987547887946 5 48 14.142519353499413 5 49 14.827475874613334 5 50 13.529145260755161 5 51 14.51337490247726 5 52 14.51150709751667 5 53 14.228756509662457 5 54 15.039217614651122 5 55 12.295839037617938 5 56 13.195656797234143 6 1 5.706737594155683 6 2 5.778433064166585 6 3 6.55873274128755 6 4 6.465872439972934 6 5 10.158756391459793 6 6 10.962956556115916 6 7 6.9278455150075615 6 8 6.367158147275951 6 9 7.277226120612546 6 10 7.51400651469676 6 11 6.368437738434292 6 12 8.082020797138899 6 13 8.01704837555236 6 14 7.814674634811148 6 15 8.468101787287122 6 16 8.685567926768876 6 17 9.253081273220094 6 18 9.880662399309095 6 19 7.9747525575143925 6 20 7.575270477731053 6 21 8.43167807601623 6 22 8.398105207910405 6 23 8.088362263562264 6 24 8.453484204268705 6 25 7.823068664696226 6 26 7.721690010964323 6 27 7.55467513682412 6 28 7.194363087698196 6 29 6.906558614632029 6 30 8.726248336906036 6 31 6.473973784036058 6 32 5.872553287926742 6 33 6.455221564968767 6 34 6.506717902639295 6 35 6.735490892422696 6 36 6.764816241648241 6 37 6.875969161858112 6 38 7.527636112977543 6 39 9.188169759916772 6 40 5.703583281810435 6 41 10.76565724158533 6 42 10.191998434989294 6 43 12.165985904207336 6 44 11.747412338975948 6 45 11.300299878434878 6 46 13.190345838235306 6 47 15.290827859831843 6 48 14.88537649635656 6 49 16.0893806365181 6 50 14.074383355993259 6 51 13.094327283429642 6 52 13.654364240373816 6 53 12.66447079537674 6 54 12.324931900365407 6 55 13.117267609046507 6 56 9.828990130567476 7 1 5.278166165584253 7 2 5.778433064166585 7 3 6.344447027001834 7 4 6.430158154258649 7 5 10.087327820031224 7 6 10.712956556115916 7 7 6.534988372150416 7 8 6.652872432990236 7 9 7.348654692041116 7 10 7.156863657553906 7 11 7.08272345272 7 12 7.653449368567468 7 13 8.659905518409499 7 14 8.100388920525432 7 15 9.11095893014426 7 16 7.97128221248316 7 17 9.57450984464867 7 18 9.809233827880526 7 19 9.224752557514392 7 20 8.218127620588199 7 21 7.824535218873368 7 22 7.86239092219612 7 23 8.409790834990833 7 24 8.73919848998299 7 25 8.358782950410511 7 26 7.8288328681071775 7 27 7.340389422538404 7 28 7.337220230555335 7 29 7.0137014717748905 7 30 8.119105479763174 7 31 6.473973784036058 7 32 6.836839002212457 7 33 6.062364422111621 7 34 6.328146474067864 7 35 5.306919463851266 7 36 7.729101955933956 7 37 7.0902548761438275 7 38 8.206207541548974 7 39 8.973884045631056 7 40 10.132154710381865 7 41 12.65851438444247 7 42 13.584855577846433 7 43 12.59455733277876 7 44 13.068840910404525 7 45 10.978871307006301 7 46 12.404631552521014 7 47 12.576542145546128 7 48 13.813947924927984 7 49 14.16080920794667 7 50 12.752954784564682 7 51 12.665755854858212 7 52 13.404364240373816 7 53 13.09304222394817 7 54 13.289217614651122 7 55 12.367267609046507 7 56 11.828990130567476 8 1 4.918276055694143 8 2 5.138323174056694 8 3 5.56422724678206 8 4 5.7213669454674445 8 5 9.50491023761364 8 6 10.075593918753281 8 7 5.1174059545679995 8 8 5.867158147275951 8 9 6.329423922810349 8 10 6.291479042169293 8 11 6.283272903269452 8 12 6.112240577358673 8 13 6.547268155772134 8 14 7.408081228217739 8 15 6.042277611462946 8 16 6.446556937757883 8 17 6.857476877615699 8 18 6.441101959748657 8 19 6.864862447624283 8 20 3.7318638843244614 8 21 5.272337416675565 8 22 6.268984328789529 8 23 6.143307318507315 8 24 6.450736951521449 8 25 5.949442291069847 8 26 6.048613087887404 8 27 4.933796015944996 8 28 4.785022428357532 8 29 6.354360812434226 8 30 5.396578007235707 8 31 4.803644113706387 8 32 6.078597243970698 8 33 4.183243542990745 8 34 4.9655091114304994 8 35 4.246479903411704 8 36 4.542288769120773 8 37 5.518826304715258 8 38 4.390273475614908 8 39 5.495862067609078 8 40 -0.48048265225549613 8 41 8.526646252574338 8 42 8.650789643780499 8 43 8.064337552558985 8 44 7.947961789525401 8 45 8.311288889423885 8 46 10.989796387685853 8 47 13.538080607084588 8 48 12.473288584268648 8 49 13.314655361792823 8 50 10.549658081267982 8 51 12.149272338374693 8 52 9.819199405208977 8 53 8.051833432739379 8 54 8.654602230035739 8 55 7.641992883771785 8 56 8.559759361336706 9 1 5.0167375941556855 9 2 5.319861635595153 9 3 6.527304169858979 9 4 6.504443868544364 9 5 10.478756391459793 9 6 11.198670841830204 9 7 6.643559800721846 9 8 6.027158147275948 9 9 6.858654692041114 9 10 5.966863657553908 9 11 7.115580595577146 9 12 7.929163654281755 9 13 8.244191232695211 9 14 7.800388920525435 9 15 8.823816073001407 9 16 8.072710783911731 9 17 8.85593841607724 9 18 8.114948113594814 9 19 7.803323986085822 9 20 7.45955619201677 9 21 8.363106647444798 9 22 8.056676636481832 9 23 8.074076549276551 9 24 8.049198489982992 9 25 8.020211521839084 9 26 7.111690010964324 9 27 6.4861037082526884 9 28 7.335791659126766 9 29 6.665130043203458 9 30 7.131962622620318 9 31 4.448259498321772 9 32 5.533981859355315 9 33 5.07093585068305 9 34 5.202432188353576 9 35 5.532633749565548 9 36 5.857673384505389 9 37 5.498826304715255 9 38 6.533350398691827 9 39 7.868169759916771 9 40 2.8764404246675817 9 41 9.312800098728182 9 42 11.347712720703576 9 43 9.867414475635908 9 44 11.240269481833096 9 45 10.59744273557773 9 46 12.917488695378157 9 47 14.396542145546128 9 48 12.842519353499416 9 49 14.780809207946668 9 50 13.85581192742183 9 51 13.240041569143926 9 52 13.251507097516672 9 53 12.848756509662454 9 54 12.499217614651123 9 55 11.875839037617936 9 56 11.808990130567473 10 1 4.790070927489019 10 2 4.885575921309439 10 3 5.773018455573265 10 4 4.4777772018777 10 5 9.158756391459793 10 6 10.141527984687347 10 7 6.058797895959941 10 8 6.105253385371192 10 9 5.812940406326831 10 10 6.692577943268191 10 11 5.808913928910481 10 12 6.974877939996038 10 13 6.600381708885688 10 14 5.981341301477812 10 15 7.3133398825252165 10 16 7.649853641054584 10 17 8.229271749410575 10 18 7.785424304071 10 19 5.831895414657254 10 20 6.825270477731053 10 21 6.098344742682894 10 22 6.48143854124374 10 23 5.350267025467023 10 24 7.53681753760204 10 25 6.132592474220029 10 26 6.3288328681071775 10 27 5.983246565395543 10 28 5.49198213531724 10 29 3.9660824241558394 10 30 5.250057860715561 10 31 4.593021403083679 10 32 3.170172335545786 10 33 4.895697755444957 10 34 4.04243218835358 10 35 4.9021575590893605 10 36 5.038625765457766 10 37 5.6854929713819224 10 38 5.194302779644207 10 39 7.759598331345341 10 40 8.54882137704853 10 41 12.253752479680564 10 42 11.03723653022739 10 43 10.987414475635905 10 44 12.021221862785474 10 45 9.205061783196783 10 46 10.511774409663875 10 47 12.362256431260413 10 48 9.528233639213699 10 49 12.946523493660955 10 50 10.848192879802777 10 51 10.737184426286781 10 52 8.678173764183338 10 53 8.557327938233882 10 54 8.920169995603501 10 55 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4.430158154258649 117 5 6.873042105745508 117 6 5.7843851275444855 117 7 5.106416943578992 117 8 4.795729575847382 117 9 5.3843689777554005 117 10 5.692577943268191 117 11 1.5470091670057151 117 12 -7.739407774289674 117 13 -5.947237338733359 117 14 -0.5424682223317099 117 15 -7.924755355570021 117 16 -9.635860644659697 117 17 -0.10406158392276055 117 18 -6.619337600690905 117 19 -1.6681045853427499 117 20 -1.031872379411805 117 21 3.2888209331590836 117 22 3.2909623507675505 117 23 3.7312194064194024 117 24 3.132055632840135 117 25 4.965925807553365 117 26 4.757404296678608 117 27 5.340389422538404 117 28 5.515791659126766 117 29 5.299415757489175 117 30 6.08339119404889 117 31 2.9739737840360583 117 32 4.336839002212457 117 33 5.062364422111621 117 34 5.756717902639295 117 35 5.235490892422696 117 36 5.943387670219671 117 37 6.447397733286689 117 38 7.027636112977543 117 39 7.616741188488202 117 40 8.453583281810435 117 41 7.8728000987281845 117 42 6.799141292132152 117 43 -5.29829981007838 117 44 -0.9311590895954787 117 45 6.514585592720589 117 46 0.011774409663875218 117 47 -0.3520292830252991 117 48 2.4568050677851296 117 49 -3.053476506339045 117 50 0.6815262131361131 117 51 8.237184426286785 117 52 7.7257928118023855 117 53 6.628756509662455 117 54 7.110646186079695 117 55 7.295839037617938 117 56 9.61470441628176 118 1 3.856737594155682 118 2 4.1331949689284855 118 3 5.020637503192312 118 4 4.677777201877696 118 5 8.892089724793127 118 6 9.598670841830202 118 7 5.6302264673885105 118 8 5.600491480609286 118 9 5.565321358707784 118 10 6.14019699088724 118 11 1.7422472622438114 118 12 -10.64416967905158 118 13 -11.142475433971452 118 14 -7.8662777461412325 118 15 -7.162850593665262 118 16 -9.673955882754935 118 17 0.8959384160772395 118 18 -9.471718553071856 118 19 -0.3300093472475112 118 20 -0.993777141316567 118 21 4.4697733141114675 118 22 5.976676636481834 118 23 4.340743215943217 118 24 5.089198489982991 118 25 4.913544855172415 118 26 5.938356677630992 118 27 6.6594370415860205 118 28 6.015791659126766 118 29 4.985130043203458 118 30 6.411962622620319 118 31 5.754926164988444 118 32 5.827315192688644 118 33 4.524269184016383 118 34 5.909098855020247 118 35 6.15930041623222 118 36 6.257673384505388 118 37 6.352159638048594 118 38 7.480017065358492 118 39 8.988169759916769 118 40 9.863107091334246 118 41 8.539466765394849 118 42 8.294379387370249 118 43 -1.7459188576974292 118 44 -0.8263971848335707 118 45 3.357442735577731 118 46 0.7308220287114935 118 47 -3.790124521120539 118 48 -0.49081397983391994 118 49 -2.205857458719997 118 50 0.029145260755161218 118 51 5.946708235810593 118 52 6.2115070975166695 118 53 5.86208984299579 118 54 6.8058842813177876 118 55 6.329172370951273 118 56 8.995656797234144 119 1 4.456737594155683 119 2 4.599861635595154 119 3 5.48730416985898 119 4 5.144443868544364 119 5 9.158756391459793 119 6 9.93200417516353 119 7 5.896893134055176 119 8 5.800491480609281 119 9 6.031988025374446 119 10 6.14019699088724 119 11 2.6089139289104786 119 12 -9.177503012384914 119 13 -1.0758087673047854 119 14 -5.132944412807902 119 15 -8.962850593665259 119 16 -15.40728921608827 119 17 -1.7707282505894284 119 18 -9.938385219738525 119 19 -1.7300093472475098 119 20 -3.993777141316567 119 21 4.669773314111463 119 22 4.376676636481832 119 23 4.7407432159432155 119 24 3.1558651566496536 119 25 4.446878188505746 119 26 7.071690010964325 119 27 6.792770374919353 119 28 6.415791659126764 119 29 6.785130043203463 119 30 6.94529595595365 119 31 6.088259498321772 119 32 6.4939818593553085 119 33 5.857602517349719 119 34 6.309098855020245 119 35 6.359300416232216 119 36 7.324340051172051 119 37 7.952159638048588 119 38 8.080017065358494 119 39 9.988169759916769 119 40 10.929773758000916 119 41 8.939466765394855 119 42 8.161046054036909 119 43 -3.212585524364094 119 44 0.440269481833095 119 45 6.357442735577731 119 46 -0.13584463795517365 119 47 -6.256791187787206 119 48 -4.290813979833921 119 49 -5.672524125386664 119 50 1.8958119274218284 119 51 6.546708235810595 119 52 6.7448404308500045 119 53 7.262089842995788 119 54 6.3392176146511225 119 55 7.595839037617939 119 56 8.862323463900811 120 1 3.3900709274890133 120 2 2.9998616355951526 120 3 3.620637503192313 120 4 4.277777201877697 120 5 7.825423058126457 120 6 9.265337508496867 120 7 4.163559800721849 120 8 4.000491480609284 120 9 4.698654692041117 120 10 4.740196990887242 120 11 2.7422472622438114 120 12 -3.310836345718247 120 13 -1.342475433971451 120 14 -3.8662777461412325 120 15 -1.4295172603319273 120 16 -10.473955882754936 120 17 1.4292717494105744 120 18 -8.005051886405191 120 19 -0.1300093472475119 120 20 0.47288952535010154 120 21 5.4697733141114675 120 22 7.443343303148502 120 23 6.074076549276551 120 24 5.755865156649655 120 25 6.713544855172412 120 26 6.405023344297653 120 27 6.459437041586025 120 28 5.082458325793436 120 29 5.451796709870127 120 30 6.211962622620323 120 31 4.354926164988438 120 32 3.6939818593553113 120 33 4.324269184016387 120 34 5.97576552168691 120 35 5.759300416232215 120 36 5.457673384505384 120 37 6.418826304715257 120 38 6.546683732025162 120 39 8.65483642658344 120 40 7.329773758000915 120 41 -4.793866567938483 120 42 -4.038953945963087 120 43 -7.47925219103076 120 44 -16.09306385150024 120 45 -4.1758905977556005 120 46 -10.00251130462184 120 47 -3.790124521120539 120 48 -7.09081397983392 120 49 -13.53919079205333 120 50 -6.037521405911505 120 51 1.2800415691439255 120 52 0.47817376418333524 120 53 0.12875650966245544 120 54 1.0058842813177868 120 55 0.8625057042846045 120 56 3.862323463900811 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 614.0 5 42.0 6 30.0 7 33.0 8 33.0 9 24.0 10 27.0 11 39.0 12 27.0 13 28.0 14 54.0 15 56.0 16 78.0 17 116.0 18 116.0 19 168.0 20 204.0 21 299.0 22 394.0 23 447.0 24 473.0 25 508.0 26 542.0 27 609.0 28 668.0 29 581.0 30 716.0 31 778.0 32 841.0 33 987.0 34 1000.0 35 1145.0 36 1306.0 37 1519.0 38 1503.0 39 1357.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.50466536113352 0.43197788273240406 0.034558230618592325 0.028798525515493608 8 99.61985946319548 0.2937449602580348 0.07487616634028338 0.011519410206197442 9 99.37219214376223 0.31678378067042967 0.23614790922704756 0.07487616634028338 10 62.492800368621126 19.91130054141228 9.808777790577123 7.787121299389471 11 40.39281188803133 22.866029259301925 18.102753139039283 18.638405713627463 12 33.70003455823062 18.20066812579196 21.783204699919363 26.316092616058057 13 26.235456744614677 13.731136965787352 24.85312751987098 35.18027876972699 14 23.626310332910954 25.031678378067042 26.49464347425412 24.847367814767885 15 31.48854961832061 18.12977099236641 25.861670136479297 24.52000925283368 16 33.12204679036533 23.97141869309669 22.957243286850293 19.94929122968768 17 29.787969578243832 19.532150265038027 30.911500345701775 19.768379811016363 18 27.4737933417809 20.32599930883539 25.624928003686207 26.5752793456975 19 27.943295107474214 25.136863942834093 20.970437388347836 25.94940356134386 20 28.915801614763552 23.166089965397923 25.945790080738178 21.972318339100347 21 26.108743232346505 21.374265637599354 24.403870521829283 28.113120608224857 22 22.19790346734247 20.23960373228891 33.68275544292133 23.879737357447297 23 24.72641400760281 21.89839880198134 27.819375647966826 25.555811542449025 24 24.19652113811773 21.662250892754294 24.703375187190417 29.43785278193756 25 26.294568285237023 23.714071770059327 25.839525372962385 24.15183457174126 26 29.54231035277842 23.472446391514872 22.68272999769426 24.30251325801245 27 22.492506340788562 24.780954576896473 22.20428867881024 30.522250403504724 28 25.81499827208847 24.703375187190417 22.595323119456285 26.886303421264827 29 31.71069327488753 24.420348367747145 19.385165532356673 24.483792825008653 30 28.484988186480724 20.31925315507405 26.784993949173053 24.410764709272172 31 31.33064516129032 23.508064516129032 22.494239631336406 22.66705069124424 32 33.32949242380596 24.48579823702253 22.24462752779858 19.940081811372934 33 31.23377747015055 21.74540001153602 23.694987598777182 23.325834919536252 34 24.802354434762535 23.5328062784927 31.167407236424488 20.49743205032027 35 23.83891997923037 25.08509779034212 31.41983499682686 19.656147233600645 36 26.662440935807307 26.529906649763745 25.279474472744035 21.528177941684916 37 29.7975428274788 23.654611524485205 25.598431101113228 20.949414546922767 38 29.407689643228263 27.439094792749103 23.028518646807527 20.1246969172151 39 29.445694180077293 24.577493222587528 20.937878525696487 25.038934071638693 40 21.988860524483638 18.908099326990023 24.373404502204686 34.729635646321654 41 19.89997673877646 17.190044196324727 21.237497092347056 41.67248197255176 42 22.60934881839718 26.0414861038886 22.412896515860634 28.936268561853584 43 19.859138667590347 22.09329176769426 26.884886271793096 31.162683292922296 44 23.647086031452357 23.06891766882516 25.150323774283073 28.133672525439408 45 26.551226551226552 23.076479076479075 26.014430014430012 24.357864357864358 46 22.030374776231447 26.81180343015534 23.7801004792978 27.377721314315412 47 18.03667495806097 22.635506449933477 25.880719615896336 33.44709897610921 48 27.891430876505503 21.569757390652914 26.74465510286406 23.794156629977525 49 22.384526558891455 21.599307159353348 25.381062355658194 30.635103926096996 50 22.939750692520775 23.90927977839335 27.625807940904895 25.52516158818098 51 22.582509681521298 23.455291601641523 27.460840413848906 26.501358302988265 52 21.83874877359035 26.051826628960583 27.78322848733191 24.326196110117156 53 22.55586995438009 25.13714846682451 28.20927412369348 24.097707455101922 54 22.401154401154404 22.58585858585859 28.83116883116883 26.181818181818183 55 21.4789139225881 23.084922010398614 28.31311380704795 27.123050259965336 56 23.184889967076764 22.75168948189222 28.365967769883905 25.697452781147117 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 2.0 6 1.5 7 1.0 8 0.5 9 0.0 10 1.5 11 3.0 12 10.0 13 22.5 14 28.0 15 25.5 16 23.0 17 27.5 18 32.0 19 30.0 20 28.0 21 27.5 22 29.0 23 31.0 24 34.5 25 38.0 26 41.5 27 45.0 28 58.0 29 74.0 30 77.0 31 90.5 32 104.0 33 125.5 34 147.0 35 163.0 36 179.0 37 209.0 38 256.0 39 273.0 40 304.5 41 336.0 42 402.0 43 468.0 44 585.0 45 702.0 46 897.5 47 1117.5 48 1142.0 49 1057.5 50 973.0 51 959.5 52 946.0 53 1027.5 54 1177.0 55 1245.0 56 1186.5 57 1128.0 58 1179.5 59 1231.0 60 1254.0 61 1277.0 62 1181.5 63 983.0 64 880.0 65 802.5 66 725.0 67 587.0 68 449.0 69 387.5 70 326.0 71 316.5 72 288.5 73 270.0 74 189.5 75 109.0 76 85.0 77 61.0 78 49.0 79 52.0 80 67.0 81 43.5 82 20.0 83 13.5 84 7.0 85 3.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.4031793572169105 16 0.04607764082478977 17 0.034558230618592325 18 0.0 19 0.051837345927888495 20 0.12671351226817187 21 0.0 22 0.0 23 0.0 24 0.0 25 0.005759705103098721 26 0.08063587144338209 27 0.08063587144338209 28 0.0 29 0.1382329224743693 30 0.051837345927888495 31 0.011519410206197442 32 0.028798525515493608 33 0.14399262757746803 34 0.1900702684022578 35 0.1670314479898629 36 0.04607764082478977 37 0.14399262757746803 38 0.23038820412394886 39 0.14399262757746803 40 0.7257228429904389 41 0.9561110471143877 42 0.31678378067042967 43 0.23038820412394886 44 0.3801405368045156 45 0.21310908881465268 46 0.25918672963944245 47 0.43197788273240406 48 0.051837345927888495 49 0.24190761433014626 50 0.1958299735053565 51 0.351342011289022 52 0.20158967860845525 53 0.25918672963944245 54 0.21310908881465268 55 0.2995046653611335 56 0.28222555005183736 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 17362.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.24916484276005 #Duplication Level Percentage of deduplicated Percentage of total 1 89.04712198031613 68.78815804630804 2 6.8222487324783785 10.54026033867066 3 1.8416343572919773 4.267941481396152 4 0.7083209066507605 2.1886879391775143 5 0.45481658216522514 1.75671005644511 6 0.275872353116612 1.2786545328879162 7 0.20131225767968985 1.0885842644856583 8 0.13420817178645988 0.8293975348462159 9 0.07456009543692216 0.5183734592788849 >10 0.3951685058156874 5.598433360211957 >50 0.03728004771846108 2.269323810620896 >100 0.007456009543692217 0.8754751756710055 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTCAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 152 0.8754751756710055 No Hit GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 94 0.5414122796912798 No Hit GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 86 0.49533463886649004 No Hit GCTCAAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 82 0.47229581845409513 No Hit GCTCAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 78 0.4492569980417003 No Hit GCTCAAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 54 0.3110240755673309 No Hit GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 38 0.21886879391775138 No Hit GCTCAAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 34 0.1958299735053565 No Hit GCTCAAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 31 0.17855085819606034 No Hit GCTCAAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 30 0.17279115309296164 No Hit GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 30 0.17279115309296164 No Hit GCTCAAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 30 0.17279115309296164 No Hit GCTCAAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 29 0.1670314479898629 No Hit GCTCAAGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG 29 0.1670314479898629 No Hit GCTCAAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 29 0.1670314479898629 No Hit GCTCAAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 28 0.16127174288676419 No Hit GCTCAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 28 0.16127174288676419 No Hit GCTCAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTG 27 0.15551203778366546 No Hit GCTCAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 26 0.14975233268056676 No Hit GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 25 0.14399262757746803 No Hit GCTCAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACAGATTGTTCTTCC 25 0.14399262757746803 No Hit GCTCAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 24 0.1382329224743693 No Hit GCTCAAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC 22 0.12671351226817187 No Hit GCTCAAGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG 21 0.12095380716507313 No Hit GCTCAAGGGGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA 21 0.12095380716507313 No Hit GCTCAAGGGGAGGTCCGGGCCTTTGACCCAGACACAGCAGCCGTGCAGCCCTACCA 19 0.10943439695887569 No Hit GCTCAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA 19 0.10943439695887569 No Hit GCTCAAGGGGTTGCGAGATCGTGGGTTTGAGTCCCACCTCGCACCTTGTTGCGAGA 18 0.10367469185577699 No Hit GCTCAAGGGGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACAT 18 0.10367469185577699 No Hit GCTCAAGGGCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG 18 0.10367469185577699 No Hit GCTCAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 18 0.10367469185577699 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.011519410206197442 0.0 0.0 0.0 0.0 15 0.011519410206197442 0.0 0.0 0.0 0.0 16 0.011519410206197442 0.0 0.0 0.0 0.0 17 0.011519410206197442 0.0 0.0 0.0 0.0 18 0.011519410206197442 0.0 0.0 0.0 0.0 19 0.028798525515493608 0.0 0.0 0.0 0.0 20 0.028798525515493608 0.0 0.0 0.0 0.0 21 0.028798525515493608 0.0 0.0 0.0 0.0 22 0.028798525515493608 0.0 0.0 0.0 0.0 23 0.034558230618592325 0.0 0.0 0.0 0.0 24 0.034558230618592325 0.0 0.0 0.0 0.0 25 0.034558230618592325 0.0 0.0 0.0 0.0 26 0.040317935721691046 0.0 0.0 0.0 0.0 27 0.051837345927888495 0.0 0.0 0.0 0.0 28 0.057597051030987216 0.0 0.0 0.0 0.0 29 0.06911646123718465 0.0 0.0 0.0 0.0 30 0.06911646123718465 0.0 0.0 0.0 0.0 31 0.07487616634028338 0.0 0.0 0.0 0.0 32 0.07487616634028338 0.0 0.0 0.0 0.0 33 0.07487616634028338 0.0 0.0 0.0 0.0 34 0.07487616634028338 0.0 0.0 0.0 0.0 35 0.08063587144338209 0.0 0.0 0.0 0.0 36 0.08639557654648082 0.0 0.0 0.0 0.0 37 0.08639557654648082 0.0 0.0 0.0 0.0 38 0.08639557654648082 0.0 0.0 0.0 0.0 39 0.08639557654648082 0.0 0.0 0.0 0.0 40 0.08639557654648082 0.0 0.0 0.0 0.0 41 0.08639557654648082 0.0 0.0 0.0 0.0 42 0.08639557654648082 0.0 0.0 0.0 0.0 43 0.09791498675267826 0.0 0.0 0.0 0.0 44 0.10367469185577699 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTCTC 15 0.008077702 50.331394 40 GCTTGTC 15 0.008077702 50.331394 38 CTTGTCT 15 0.008077702 50.331394 39 GCAAAAG 15 0.008171324 50.185505 35 AAGATTA 25 1.9930976E-5 50.040466 48 AGATTAA 25 1.9930976E-5 50.040466 49 AAGCAGT 25 1.9930976E-5 50.040466 10 ACCTGGT 20 4.0535734E-4 50.040462 11 ATATGCT 20 4.0535734E-4 50.040462 34 TCTCAAA 20 4.0535734E-4 50.040462 43 AAAGATT 20 4.0535734E-4 50.040462 47 CCTGGTT 20 4.0535734E-4 50.040462 12 GTACCTG 30 9.832438E-7 50.04046 9 GCGGCTT 15 0.008265751 50.04046 50 GAAGCAG 30 9.832438E-7 50.04046 9 AGGGGTC 45 1.2369128E-10 49.89626 6 AGGGGCT 90 0.0 49.89626 6 AGGGGCG 25 2.0276657E-5 49.896255 6 AGGGGAT 50 5.456968E-12 49.896255 6 CCAGTAG 25 2.0276657E-5 49.896255 26 >>END_MODULE