FastQCFastQC Report
Fri 17 Jun 2016
SRR1525893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511
Sequences flagged as poor quality0
Sequence length40
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGA221.455989410986102No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCCGA140.9265387160820648No Hit
GCTCAAGGGAATTGACGGGTACGCACCGTTCTGAGTCGGA130.8603573792190603No Hit
GCTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCCGA80.5294506949040371No Hit
GCTCAAGGGAATTGACGGGTACGCACCGTTCTGAGTCCGA70.4632693580410324No Hit
GCTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGGA70.4632693580410324No Hit
GCTCAAGGGGCCGAATAGCCTCTCCACCCCAGCGGCCCGA50.3309066843150232No Hit
GCTCAAAGGAATTGACGGGTACGCACCGTTCTGAGTCGGA50.3309066843150232No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGCGTCGGA50.3309066843150232No Hit
GCTCAAGGGGATTGATTTGGTGTTCTGTATGTTCTTTTTA50.3309066843150232No Hit
GCTCAAGGGGAGTCCCTTCTTCTGAGAAGGGAAAACATTG40.26472534745201853No Hit
GCTCAAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGC30.1985440105890139No Hit
GCTCAAGGGGCGATAGGAGTGGCACCGTGGATCCCCCGGA30.1985440105890139No Hit
GCTCAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGG30.1985440105890139No Hit
GCTCAAGGGAGGTTGAAGTGGTTTGTCATATGCTTTGTTG30.1985440105890139No Hit
GCTCAAGGGGATCCAGTGACAGATACCATTAGATTCAAGA30.1985440105890139No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCCGAGTCGGA20.13236267372600927No Hit
GCTCAAGGGGAGTCTTTTGCCTCAGACGCTAGCTGTAGCT20.13236267372600927No Hit
GCTCAAGGGTGTCAGTGAGCTAACTATTCTTGTCCAGAAA20.13236267372600927No Hit
GCTCAAGGGGATCCAGTGACAGATACCATTAGATTCCCGA20.13236267372600927No Hit
GCTCAAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGA20.13236267372600927No Hit
GCTCAAGGGGTTCTTTTTTCTTTCTTTTTTTTTTCCTTTT20.13236267372600927No Hit
GCTCAAGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGC20.13236267372600927No Hit
GCTCAAGGGCACGCAGATCGACTTTTTTTTTTTTTTTTTT20.13236267372600927No Hit
GCTCAAGGGGGCTCTAGCCATCCTCAGCATCCTGTAGTAT20.13236267372600927No Hit
GCTCAAGGGAGCAAATGCTGAACCGTGTGTCCTTCCTTTG20.13236267372600927No Hit
GCTCAAGGGAGAAAAAAATGTTTGTTTTTGGTTCTCTGTG20.13236267372600927No Hit
GCTCAAGGGGGGGCTGGACAGATGGCTCCGGGGTTACGAG20.13236267372600927No Hit
GCTCAAGGGAATTGACGGGTACGCACCGTTCCGAGTCGGC20.13236267372600927No Hit
GCTCAAGGGTGCAGAATTCAGTTGAAGTTGACATTCTTTT20.13236267372600927No Hit
GCTCAAGGGGCCGAATAGCCTCTCCACCCACGCCGCCCGA20.13236267372600927No Hit
GCTCAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTTAA20.13236267372600927No Hit
GCTCAAGGGGGTAGGAACCTGAGGGAGCTAACTGAAGCCT20.13236267372600927No Hit
GCTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCCGA20.13236267372600927No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCCGC20.13236267372600927No Hit
GCTCAAGGGGGAAAAAACCTTTAATAGTGAATAATTAACA20.13236267372600927No Hit
GCTCAAGGGAATTGACGGGTACGCACCGTTCTGCGTCCGA20.13236267372600927No Hit
GCTCAAGGGAAGCATGGTTCAACGCAGAGTCGACTTTTTT20.13236267372600927No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC20.13236267372600927No Hit
GCTCAAGGGTCACAGAGTTTTTTTTTTTTTTTTTTTTTTT20.13236267372600927No Hit
GCTCAAGGGAATTGACGGGTACGCCCAGTTCTGAGTCGGA20.13236267372600927No Hit
GCTCAAGGGGCTCTCTCCGCCATCTTGGCGCCTGTGGAGG20.13236267372600927No Hit
GCTCAAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA20.13236267372600927No Hit
GCTCAAGGGATTTAGCTGGTGCTTCAAGCCACCGTTTGAA20.13236267372600927No Hit
GCTCAAGGGGTTCAGACCAAACGTCGGGCCCCGGAGAAGA20.13236267372600927No Hit
GCTCAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT20.13236267372600927No Hit
GCTCAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCT20.13236267372600927No Hit
GCTCAAGGGAAGCAGGGATGAGATGGACTTAAATGAATTG20.13236267372600927No Hit
GCTCAAGGGGGATGGTTTGAGTTAGGATCTTTTTGCATTT20.13236267372600927No Hit
GCTCAAGGGGAATTTGAGTACCCTAAGTGCCTGGTATATG20.13236267372600927No Hit
GCTCAAGGGCTCGTCTTAATCAGCAACATCTTTTGATAAA20.13236267372600927No Hit
GCTCAAGGGGGGAATTCCCTCGATTCTGTTTTGCTACCCG20.13236267372600927No Hit
GCTCAAAGGAATTGACGGGTACGCACAGTTCTGCGTCGGA20.13236267372600927No Hit
GCTCAAGGGAGAAAGATGGGGTCTTAAGTTTTGATTGGAT20.13236267372600927No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACC20.13236267372600927No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC20.13236267372600927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGAA251.02090984E-434.9310385
CAAGGGG504.1836756E-1134.9310384
TCAAGGG1200.034.9310343
AAGGGGA200.001725869534.9310345
AGGGAAT200.001725869534.9310346
CAAGGGA458.62201E-1034.9310344
GCTCAAG1300.034.9310341
CTCAAGG1200.034.9310342
GGGTACG251.2707495E-433.76666617
GACGGGT354.353933E-733.76666614
CGGGTAC307.5699445E-633.76666616
TACGCAC251.2707495E-433.76666620
ACGGGTA307.5699445E-633.76666615
GGAATTG354.353933E-733.7666668
GGTACGC251.2707495E-433.76666618
TGACGGG354.353933E-733.76666613
GTACGCA251.2707495E-433.76666619
CTGCGTC200.002064244133.76666331
TCTGCGT200.002064244133.76666330
TTGACGG402.4114343E-833.76666312
Produced by FastQC (version 0.11.5)