##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525893_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1511
Sequences flagged as poor quality	0
Sequence length	40
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.221707478491066	29.0	22.0	31.0	4.0	33.0
2	11.302448709463931	11.0	9.0	11.0	4.0	17.0
3	14.531436135009928	15.0	11.0	17.0	4.0	26.0
4	15.879549966909332	13.0	9.0	25.0	4.0	29.0
5	18.072137657180676	19.0	10.0	26.0	4.0	31.0
6	20.207809397749834	22.0	10.0	29.0	4.0	33.0
7	20.059563203176705	23.0	10.0	29.0	4.0	33.0
8	21.26935804103243	26.0	11.0	29.0	4.0	33.0
9	19.780939774983455	23.0	9.0	28.0	4.0	32.0
10	18.164791528788882	20.0	9.0	26.0	4.0	31.0
11	23.38848444738584	28.0	10.0	35.0	4.0	38.0
12	22.911317008603575	27.0	10.0	35.0	4.0	38.0
13	23.017207147584383	27.0	4.0	34.0	4.0	37.0
14	24.049636002647254	30.0	4.0	35.0	4.0	38.0
15	22.24751819986764	27.0	4.0	35.0	4.0	37.0
16	19.75181998676373	21.0	4.0	32.0	4.0	36.0
17	21.015221707478492	26.0	4.0	33.0	4.0	36.0
18	20.824619457313037	26.0	4.0	33.0	4.0	36.0
19	18.15751158173395	18.0	4.0	31.0	4.0	35.0
20	19.213103904698876	22.0	4.0	32.0	4.0	36.0
21	16.500992720052945	14.0	4.0	30.0	4.0	34.0
22	16.326935804103243	12.0	4.0	30.0	4.0	34.0
23	16.198544010589014	11.0	4.0	30.0	4.0	34.0
24	15.260092653871608	4.0	4.0	29.0	4.0	33.0
25	14.964262078093977	4.0	4.0	29.0	4.0	34.0
26	11.972203838517538	4.0	4.0	22.0	4.0	31.0
27	11.823295830575777	4.0	4.0	22.0	4.0	31.0
28	11.485109199205825	4.0	4.0	20.0	4.0	31.0
29	10.972865651886169	4.0	4.0	19.0	4.0	31.0
30	10.333553937789544	4.0	4.0	15.0	4.0	30.0
31	10.026472534745201	4.0	4.0	14.0	4.0	31.0
32	9.43481138318994	4.0	4.0	4.0	4.0	30.0
33	8.930509596293845	4.0	4.0	4.0	4.0	28.0
34	8.644606221045665	4.0	4.0	4.0	4.0	28.0
35	8.095301125082727	4.0	4.0	4.0	4.0	26.0
36	8.00595632031767	4.0	4.0	4.0	4.0	27.0
37	7.698874917273329	4.0	4.0	4.0	4.0	26.0
38	7.651886168100596	4.0	4.0	4.0	4.0	26.0
39	7.28788881535407	4.0	4.0	4.0	4.0	22.0
40	7.42819324950364	4.0	4.0	4.0	4.0	24.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	4.790963855421687
1	2	23.510843373493977
1	3	17.84417670682731
1	4	15.768875502008031
1	5	12.830120481927707
1	6	11.394377510040165
1	7	13.620481927710845
1	8	10.56546184738956
1	9	8.305622489959841
1	10	11.1144578313253
1	11	0.8110441767068295
1	12	4.0036144578313255
1	13	-2.5929718875502026
1	14	6.703012048192772
1	15	8.753212851405621
1	16	0.808032128514057
1	17	-0.520080321285139
1	18	3.1006024096385545
1	19	4.078112449799198
1	20	4.026706827309237
1	21	9.14417670682731
1	22	3.115060240963855
1	23	5.592369477911644
1	24	4.719076305220884
1	25	4.097590361445782
1	26	7.316666666666666
1	27	8.316064257028113
1	28	14.797590361445783
1	29	7.231927710843372
1	30	7.462449799196786
1	31	8.5214859437751
1	32	7.509839357429717
1	33	3.7170682730923694
1	34	3.3357429718875515
1	35	-3.4228915662650587
1	36	-3.9287148594377514
1	37	-2.8785140562248994
1	38	-3.306024096385543
1	39	-2.673694779116466
1	40	-2.792369477911646
2	1	-0.9590361445783131
2	2	9.510843373493975
2	3	-3.1558232931726913
2	4	16.76887550200803
2	5	-12.169879518072293
2	6	-14.605622489959835
2	7	-14.379518072289155
2	8	-14.68453815261044
2	9	-14.694377510040159
2	10	-13.1355421686747
2	11	-18.93895582329317
2	12	-18.246385542168674
2	13	-18.092971887550203
2	14	-19.29698795180723
2	15	-17.24678714859438
2	16	-15.191967871485943
2	17	-15.770080321285139
2	18	-15.649397590361446
2	19	-11.421887550200802
2	20	-13.973293172690763
2	21	-10.10582329317269
2	22	-10.384939759036145
2	23	-9.907630522088356
2	24	-9.280923694779116
2	25	-10.402409638554218
2	26	-7.183333333333334
2	27	-6.433935742971887
2	28	-5.952409638554217
2	29	-6.018072289156628
2	30	-4.787550200803214
2	31	-5.478514056224901
2	32	-5.240160642570283
2	33	-4.032931726907631
2	34	-4.4142570281124485
2	35	-3.4228915662650587
2	36	-3.9287148594377514
2	37	-2.8785140562248994
2	38	-3.306024096385543
2	39	-2.673694779116466
2	40	-2.792369477911646
5	1	-10.459036144578313
5	2	-1.9891566265060252
5	3	-0.15582329317269128
5	4	-7.231124497991969
5	5	-4.1698795180722925
5	6	-5.605622489959835
5	7	-10.879518072289155
5	8	-9.18453815261044
5	9	-11.694377510040159
5	10	-5.6355421686747
5	11	-5.9389558232931705
5	12	-3.7463855421686745
5	13	-3.5929718875502026
5	14	-5.796987951807228
5	15	-1.746787148594379
5	16	1.808032128514057
5	17	-9.270080321285139
5	18	-0.1493975903614455
5	19	4.078112449799198
5	20	3.026706827309237
5	21	6.894176706827309
5	22	3.615060240963855
5	23	5.592369477911644
5	24	4.719076305220884
5	25	3.597590361445782
5	26	-7.183333333333334
5	27	-6.433935742971887
5	28	-5.952409638554217
5	29	-6.018072289156628
5	30	-4.787550200803214
5	31	-5.478514056224901
5	32	-5.240160642570283
5	33	-4.032931726907631
5	34	-4.4142570281124485
5	35	-3.4228915662650587
5	36	-3.9287148594377514
5	37	-2.8785140562248994
5	38	-3.306024096385543
5	39	-2.673694779116466
5	40	-2.792369477911646
6	1	1.040963855421687
6	2	0.5108433734939748
6	3	-2.1558232931726913
6	4	-4.231124497991969
6	5	-11.169879518072293
6	6	4.394377510040165
6	7	-0.37951807228915513
6	8	-8.68453815261044
6	9	-4.694377510040159
6	10	-7.1355421686747
6	11	10.06104417670683
6	12	10.753614457831326
6	13	9.907028112449797
6	14	9.703012048192772
6	15	12.753212851405621
6	16	7.808032128514057
6	17	10.229919678714861
6	18	8.350602409638554
6	19	1.5781124497991978
6	20	15.026706827309237
6	21	-4.105823293172691
6	22	9.615060240963855
6	23	4.092369477911644
6	24	11.719076305220884
6	25	10.597590361445782
6	26	18.816666666666666
6	27	3.5660642570281134
6	28	20.047590361445785
6	29	22.981927710843372
6	30	-4.787550200803214
6	31	-5.478514056224901
6	32	-5.240160642570283
6	33	-4.032931726907631
6	34	-4.4142570281124485
6	35	-3.4228915662650587
6	36	-3.9287148594377514
6	37	-2.8785140562248994
6	38	-3.306024096385543
6	39	-2.673694779116466
6	40	-2.792369477911646
7	1	3.540963855421687
7	2	0.5108433734939748
7	3	-0.15582329317269128
7	4	10.268875502008031
7	5	2.8301204819277075
7	6	4.894377510040165
7	7	2.120481927710845
7	8	-1.1845381526104397
7	9	1.3056224899598412
7	10	12.8644578313253
7	11	4.0610441767068295
7	12	6.2536144578313255
7	13	5.407028112449797
7	14	4.703012048192772
7	15	11.253212851405621
7	16	5.808032128514057
7	17	12.729919678714861
7	18	3.3506024096385545
7	19	5.578112449799198
7	20	12.026706827309237
7	21	0.39417670682730943
7	22	10.615060240963855
7	23	8.592369477911644
7	24	13.219076305220884
7	25	12.097590361445782
7	26	3.8166666666666664
7	27	9.566064257028113
7	28	-0.4524096385542169
7	29	-2.0180722891566276
7	30	-2.2875502008032136
7	31	7.021485943775099
7	32	0.7598393574297173
7	33	-1.0329317269076306
7	34	3.0857429718875515
7	35	9.577108433734942
7	36	9.07128514056225
7	37	4.121485943775101
7	38	7.693975903614457
7	39	11.326305220883533
7	40	12.207630522088355
9	1	4.707630522088355
9	2	2.510843373493975
9	3	2.1775100401606426
9	4	2.435542168674699
9	5	10.496787148594375
9	6	5.061044176706833
9	7	6.287148594377513
9	8	2.982128514056228
9	9	4.972289156626509
9	10	5.531124497991968
9	11	9.06104417670683
9	12	4.7536144578313255
9	13	3.5736947791164653
9	14	7.703012048192772
9	15	10.419879518072289
9	16	9.14136546184739
9	17	9.896586345381529
9	18	13.350602409638554
9	19	20.5781124497992
9	20	6.026706827309237
9	21	-2.7724899598393566
9	22	-0.3849397590361452
9	23	-2.574297188755022
9	24	1.7190763052208844
9	25	0.597590361445782
9	26	0.8166666666666664
9	27	2.2327309236947794
9	28	-4.619076305220884
9	29	1.6485943775100385
9	30	0.8791164658634525
9	31	0.5214859437750992
9	32	-3.5734939759036157
9	33	0.9670682730923694
9	34	0.5857429718875515
9	35	2.5771084337349413
9	36	6.4046184738955825
9	37	7.4548192771084345
9	38	1.3606425702811231
9	39	5.659638554216868
9	40	5.540963855421688
10	1	5.240963855421686
10	2	0.9108433734939752
10	3	13.044176706827308
10	4	9.568875502008032
10	5	2.8301204819277075
10	6	4.5943775100401645
10	7	3.4204819277108456
10	8	3.715461847389559
10	9	2.105622489959842
10	10	4.464457831325301
10	11	7.86104417670683
10	12	13.353614457831327
10	13	7.907028112449797
10	14	3.903012048192771
10	15	12.753212851405621
10	16	7.008032128514056
10	17	7.229919678714861
10	18	10.150602409638555
10	19	12.378112449799199
10	20	14.426706827309236
10	21	12.89417670682731
10	22	11.815060240963854
10	23	15.892369477911645
10	24	13.319076305220886
10	25	6.397590361445783
10	26	0.6166666666666671
10	27	2.166064257028113
10	28	-3.152409638554217
10	29	2.9819277108433724
10	30	0.4124497991967857
10	31	5.3214859437751
10	32	4.359839357429717
10	33	0.3670682730923698
10	34	4.385742971887552
10	35	1.177108433734941
10	36	7.0712851405622486
10	37	6.121485943775101
10	38	2.0939759036144574
10	39	3.326305220883534
10	40	1.8076305220883535
11	1	3.040963855421687
11	2	7.010843373493975
11	3	-0.15582329317269128
11	4	-2.731124497991969
11	5	-5.6698795180722925
11	6	-0.10562248995983481
11	7	6.620481927710845
11	8	7.31546184738956
11	9	5.805622489959841
11	10	3.8644578313253
11	11	7.5610441767068295
11	12	4.2536144578313255
11	13	-1.5929718875502026
11	14	7.203012048192772
11	15	-6.246787148594379
11	16	10.308032128514057
11	17	9.729919678714861
11	18	7.8506024096385545
11	19	5.578112449799198
11	20	11.026706827309237
11	21	4.894176706827309
11	22	14.115060240963855
11	23	17.592369477911646
11	24	15.719076305220884
11	25	14.597590361445782
11	26	0.31666666666666643
11	27	-0.9339357429718866
11	28	3.047590361445783
11	29	-0.01807228915662762
11	30	7.712449799196786
11	31	4.021485943775099
11	32	6.259839357429717
11	33	5.467068273092369
11	34	4.0857429718875515
11	35	5.077108433734941
11	36	7.0712851405622486
11	37	9.1214859437751
11	38	9.193975903614458
11	39	10.326305220883533
11	40	9.707630522088355
12	1	-4.292369477911645
12	2	-1.8224899598393591
12	3	-3.1558232931726913
12	4	-6.564457831325302
12	5	-0.8365461847389586
12	6	0.06104417670683304
12	7	-3.712851405622489
12	8	1.6487951807228924
12	9	-5.027710843373493
12	10	1.1977911646586321
12	11	4.394377510040162
12	12	4.420281124497993
12	13	0.9070281124497974
12	14	-0.29698795180722826
12	15	6.086546184738953
12	16	7.474698795180725
12	17	4.229919678714861
12	18	6.017269076305222
12	19	8.91144578313253
12	20	6.360040160642569
12	21	8.560843373493977
12	22	5.281726907630523
12	23	3.759036144578312
12	24	4.0524096385542165
12	25	-2.402409638554218
12	26	1.8166666666666664
12	27	2.5660642570281134
12	28	2.047590361445783
12	29	2.9819277108433724
12	30	4.212449799196786
12	31	3.1881526104417652
12	32	1.0931726907630512
12	33	-1.0329317269076306
12	34	4.252409638554218
12	35	-3.4228915662650587
12	36	-3.9287148594377514
12	37	-2.8785140562248994
12	38	-3.306024096385543
12	39	-2.673694779116466
12	40	-2.792369477911646
15	1	1.790963855421687
15	2	11.260843373493975
15	3	1.8441767068273087
15	4	-0.23112449799196888
15	5	7.8301204819277075
15	6	5.644377510040165
15	7	10.120481927710845
15	8	9.06546184738956
15	9	8.055622489959841
15	10	6.3644578313253
15	11	13.56104417670683
15	12	13.753614457831326
15	13	10.157028112449797
15	14	10.203012048192772
15	15	10.253212851405621
15	16	10.808032128514057
15	17	11.979919678714861
15	18	10.350602409638554
15	19	20.3281124497992
15	20	15.026706827309237
15	21	16.39417670682731
15	22	8.615060240963855
15	23	15.342369477911644
15	24	8.719076305220884
15	25	14.347590361445782
15	26	11.066666666666666
15	27	5.316064257028113
15	28	8.547590361445783
15	29	10.981927710843372
15	30	10.712449799196786
15	31	5.271485943775099
15	32	14.009839357429717
15	33	13.21706827309237
15	34	15.335742971887552
15	35	11.327108433734942
15	36	8.32128514056225
15	37	2.6214859437751006
15	38	4.443975903614457
15	39	9.826305220883533
15	40	3.957630522088354
16	1	1.7076305220883548
16	2	0.5108433734939748
16	3	0.17751004016064265
16	4	6.102208835341363
16	5	1.4967871485943753
16	6	-3.9389558232931687
16	7	-1.046184738955823
16	8	4.648795180722892
16	9	-2.027710843373491
16	10	5.8644578313253
16	11	11.06104417670683
16	12	5.086947791164658
16	13	10.907028112449797
16	14	4.703012048192772
16	15	12.753212851405621
16	16	3.474698795180725
16	17	2.563253012048193
16	18	-1.3160642570281134
16	19	1.91144578313253
16	20	6.693373493975905
16	21	-10.10582329317269
16	22	-10.384939759036145
16	23	-9.907630522088356
16	24	-9.280923694779116
16	25	-10.402409638554218
16	26	-7.183333333333334
16	27	-6.433935742971887
16	28	-5.952409638554217
16	29	-6.018072289156628
16	30	-4.787550200803214
16	31	-5.478514056224901
16	32	-5.240160642570283
16	33	-4.032931726907631
16	34	-4.4142570281124485
16	35	-3.4228915662650587
16	36	-3.9287148594377514
16	37	-2.8785140562248994
16	38	-3.306024096385543
16	39	-2.673694779116466
16	40	-2.792369477911646
17	1	5.707630522088355
17	2	7.177510040160643
17	3	2.510843373493975
17	4	7.435542168674699
17	5	5.496787148594375
17	6	11.394377510040165
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108	34	-4.4142570281124485
108	35	-3.4228915662650587
108	36	-3.9287148594377514
108	37	-2.8785140562248994
108	38	-3.306024096385543
108	39	-2.673694779116466
108	40	-2.792369477911646
109	1	-6.959036144578313
109	2	-3.489156626506025
109	3	-3.6558232931726913
109	4	-4.731124497991969
109	5	-3.6698795180722925
109	6	-2.105622489959835
109	7	-2.879518072289155
109	8	-2.6845381526104397
109	9	-2.194377510040159
109	10	-8.6355421686747
109	11	-2.9389558232931705
109	12	-3.2463855421686745
109	13	-3.0929718875502026
109	14	-2.7969879518072283
109	15	-0.7467871485943789
109	16	-8.691967871485943
109	17	-2.270080321285139
109	18	-12.649397590361446
109	19	-1.9218875502008022
109	20	-1.473293172690763
109	21	-5.605823293172691
109	22	2.115060240963855
109	23	0.09236947791164418
109	24	-3.7809236947791156
109	25	-2.402409638554218
109	26	2.3166666666666664
109	27	-2.4339357429718866
109	28	7.547590361445783
109	29	7.981927710843372
109	30	8.712449799196786
109	31	11.0214859437751
109	32	11.259839357429717
109	33	9.46706827309237
109	34	9.085742971887552
109	35	9.577108433734942
109	36	2.5712851405622486
109	37	3.6214859437751006
109	38	14.193975903614458
109	39	3.826305220883534
109	40	3.707630522088354
110	1	6.040963855421687
110	2	0.010843373493974795
110	3	1.8441767068273087
110	4	-4.731124497991969
110	5	2.3301204819277075
110	6	6.394377510040165
110	7	-0.8795180722891551
110	8	10.31546184738956
110	9	6.805622489959841
110	10	-2.6355421686747
110	11	11.56104417670683
110	12	12.253614457831326
110	13	-3.0929718875502026
110	14	-5.296987951807228
110	15	-1.246787148594379
110	16	-3.191967871485943
110	17	-1.270080321285139
110	18	-10.649397590361446
110	19	-5.421887550200802
110	20	-1.973293172690763
110	21	1.8941767068273094
110	22	0.6150602409638548
110	23	4.592369477911644
110	24	5.219076305220884
110	25	4.097590361445782
110	26	8.316666666666666
110	27	9.066064257028113
110	28	11.047590361445783
110	29	10.981927710843372
110	30	-4.787550200803214
110	31	-5.478514056224901
110	32	-5.240160642570283
110	33	-4.032931726907631
110	34	-4.4142570281124485
110	35	-3.4228915662650587
110	36	-3.9287148594377514
110	37	-2.8785140562248994
110	38	-3.306024096385543
110	39	-2.673694779116466
110	40	-2.792369477911646
112	1	6.040963855421687
112	2	13.010843373493975
112	3	5.844176706827309
112	4	9.768875502008031
112	5	6.8301204819277075
112	6	1.3943775100401652
112	7	-0.8795180722891551
112	8	9.81546184738956
112	9	6.305622489959841
112	10	3.3644578313253
112	11	3.0610441767068295
112	12	-0.7463855421686745
112	13	8.907028112449797
112	14	8.203012048192772
112	15	13.253212851405621
112	16	8.308032128514057
112	17	6.229919678714861
112	18	2.3506024096385545
112	19	0.5781124497991978
112	20	8.526706827309237
112	21	-2.1058232931726906
112	22	-3.884939759036145
112	23	-4.407630522088356
112	24	-6.780923694779116
112	25	1.097590361445782
112	26	-4.183333333333334
112	27	5.566064257028113
112	28	4.047590361445783
112	29	4.481927710843372
112	30	8.712449799196786
112	31	9.0214859437751
112	32	7.759839357429717
112	33	8.96706827309237
112	34	0.08574297188755153
112	35	9.577108433734942
112	36	11.57128514056225
112	37	2.6214859437751006
112	38	2.193975903614457
112	39	2.826305220883534
112	40	2.707630522088354
114	1	-1.959036144578313
114	2	0.5108433734939748
114	3	0.8441767068273087
114	4	-4.231124497991969
114	5	10.830120481927707
114	6	-1.6056224899598348
114	7	-0.37951807228915513
114	8	4.31546184738956
114	9	-3.694377510040159
114	10	2.8644578313253
114	11	7.0610441767068295
114	12	-7.2463855421686745
114	13	-7.092971887550203
114	14	-2.2969879518072283
114	15	4.753212851405621
114	16	-8.191967871485943
114	17	-9.770080321285139
114	18	-2.6493975903614455
114	19	10.578112449799198
114	20	-1.973293172690763
114	21	15.89417670682731
114	22	15.615060240963855
114	23	23.092369477911646
114	24	23.719076305220884
114	25	22.597590361445782
114	26	-7.183333333333334
114	27	-6.433935742971887
114	28	-5.952409638554217
114	29	-6.018072289156628
114	30	-4.787550200803214
114	31	-5.478514056224901
114	32	-5.240160642570283
114	33	-4.032931726907631
114	34	-4.4142570281124485
114	35	-3.4228915662650587
114	36	-3.9287148594377514
114	37	-2.8785140562248994
114	38	-3.306024096385543
114	39	-2.673694779116466
114	40	-2.792369477911646
120	1	5.040963855421687
120	2	8.510843373493975
120	3	12.844176706827309
120	4	-4.231124497991969
120	5	-7.1698795180722925
120	6	-8.605622489959835
120	7	-2.379518072289155
120	8	-3.6845381526104397
120	9	6.305622489959841
120	10	9.8644578313253
120	11	-0.9389558232931705
120	12	2.7536144578313255
120	13	-5.092971887550203
120	14	7.703012048192772
120	15	2.753212851405621
120	16	4.808032128514057
120	17	7.229919678714861
120	18	13.350602409638554
120	19	6.578112449799198
120	20	6.026706827309237
120	21	21.89417670682731
120	22	22.615060240963857
120	23	-4.907630522088356
120	24	13.719076305220884
120	25	22.597590361445782
120	26	-7.183333333333334
120	27	-6.433935742971887
120	28	-5.952409638554217
120	29	-6.018072289156628
120	30	-4.787550200803214
120	31	-5.478514056224901
120	32	-5.240160642570283
120	33	-4.032931726907631
120	34	-4.4142570281124485
120	35	-3.4228915662650587
120	36	-3.9287148594377514
120	37	-2.8785140562248994
120	38	-3.306024096385543
120	39	-2.673694779116466
120	40	-2.792369477911646
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	322.0
5	7.0
6	7.0
7	6.0
8	10.0
9	21.0
10	38.0
11	54.0
12	68.0
13	45.0
14	72.0
15	74.0
16	81.0
17	90.0
18	68.0
19	92.0
20	70.0
21	57.0
22	56.0
23	69.0
24	55.0
25	49.0
26	36.0
27	21.0
28	17.0
29	13.0
30	6.0
31	5.0
32	1.0
33	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	94.30086149768059	5.168986083499006	0.06626905235255136	0.46388336646785955
8	98.27928524156188	0.727994705493051	0.1985440105890139	0.7941760423560555
9	99.33774834437085	0.1986754966887417	0.33112582781456956	0.13245033112582782
10	46.65784248841827	38.78226340172071	7.1475843812045	7.412309728656519
11	27.597617471872933	41.49569821310391	15.023163467902052	15.883520847121114
12	31.89940436796823	11.780277961614823	37.32627399073461	18.994043679682328
13	23.560555923229646	16.015883520847122	37.789543348775645	22.634017207147583
14	33.75248180013236	24.75181998676373	24.090006618133685	17.405691594970218
15	19.589675711449374	36.46591661151555	25.01654533421575	18.927862342819324
16	24.553275976174717	14.692256783587029	25.6783587028458	35.07610853739245
17	39.17935142289875	20.25148908007942	21.575115817339512	18.994043679682328
18	40.70152217074785	18.067504963600264	19.523494374586367	21.70747849106552
19	34.10749834107498	13.536828135368282	16.39017916390179	35.96549435965494
20	20.648577101257445	21.641297154202515	35.407015221707475	22.30311052283256
21	20.383851753805427	32.56121773659828	23.95764394440768	23.097286565188615
22	18.199867637326275	18.266048974189278	21.111846459298476	42.42223692918597
23	34.149569821310386	17.207147584381204	24.553275976174717	24.090006618133685
24	19.5364238410596	19.205298013245034	22.450331125827812	38.80794701986755
25	21.39072847682119	31.65562913907285	18.741721854304636	28.211920529801326
26	17.356475300400533	19.425901201602137	23.2977303070761	39.91989319092123
27	19.457313037723363	23.95764394440768	22.236929185969558	34.348113831899404
28	29.337748344370862	17.483443708609272	24.17218543046358	29.00662251655629
29	18.43501326259947	17.771883289124666	36.07427055702917	27.718832891246688
30	17.58460517584605	13.470471134704711	42.003981420039814	26.940942269409422
31	15.583554376657824	17.307692307692307	29.244031830238725	37.86472148541114
32	23.856858846918488	12.19350563286945	41.4181577203446	22.53147779986746
33	29.9801455989411	13.43481138318994	31.237590999338188	25.347452018530774
34	16.082064857710126	26.207809397749838	32.56121773659828	25.148908007941763
35	30.57577763070814	16.28060886829914	32.36267372600927	20.78093977498345
36	13.500992720052945	15.684976836532098	45.929847782925215	24.884182660489742
37	14.90066225165563	15.165562913907285	30.86092715231788	39.0728476821192
38	23.759099933818664	15.221707478491064	29.71542025148908	31.30377233620119
39	30.311052283256124	15.817339510258108	30.443414956982128	23.42819324950364
40	16.545334215751158	29.252150893448047	29.252150893448047	24.950363997352746
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	1.0
17	1.0
18	1.0
19	1.5
20	2.0
21	3.0
22	4.0
23	4.0
24	7.5
25	11.0
26	12.0
27	13.0
28	13.0
29	17.0
30	21.0
31	20.5
32	20.0
33	20.0
34	26.5
35	33.0
36	36.5
37	40.0
38	40.0
39	48.0
40	56.0
41	60.0
42	64.0
43	64.0
44	72.0
45	80.0
46	74.0
47	68.0
48	68.0
49	76.0
50	84.0
51	101.0
52	118.0
53	118.0
54	134.0
55	150.0
56	156.0
57	162.0
58	162.0
59	147.0
60	132.0
61	117.0
62	102.0
63	102.0
64	108.5
65	115.0
66	102.5
67	90.0
68	90.0
69	77.5
70	65.0
71	54.5
72	44.0
73	44.0
74	33.5
75	23.0
76	15.5
77	8.0
78	8.0
79	5.0
80	2.0
81	1.5
82	1.0
83	1.0
84	1.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.13236267372600927
8	0.0
9	0.06618133686300463
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.26472534745201853
20	0.0
21	0.0
22	0.0
23	0.0
24	0.06618133686300463
25	0.06618133686300463
26	0.8603573792190603
27	0.0
28	0.06618133686300463
29	0.1985440105890139
30	0.26472534745201853
31	0.1985440105890139
32	0.13236267372600927
33	0.0
34	0.0
35	0.0
36	0.0
37	0.06618133686300463
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	1511.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.13170086035738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.93318809005083	87.42554599602911
2	2.904865649963689	5.294506949040371
3	0.36310820624546114	0.9927200529450696
4	0.07262164124909223	0.26472534745201853
5	0.29048656499636893	1.3236267372600927
6	0.0	0.0
7	0.14524328249818447	0.9265387160820648
8	0.07262164124909223	0.5294506949040371
9	0.0	0.0
>10	0.2178649237472767	3.242885506287227
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGA	22	1.455989410986102	No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCCGA	14	0.9265387160820648	No Hit
GCTCAAGGGAATTGACGGGTACGCACCGTTCTGAGTCGGA	13	0.8603573792190603	No Hit
GCTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCCGA	8	0.5294506949040371	No Hit
GCTCAAGGGAATTGACGGGTACGCACCGTTCTGAGTCCGA	7	0.4632693580410324	No Hit
GCTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGGA	7	0.4632693580410324	No Hit
GCTCAAGGGGCCGAATAGCCTCTCCACCCCAGCGGCCCGA	5	0.3309066843150232	No Hit
GCTCAAAGGAATTGACGGGTACGCACCGTTCTGAGTCGGA	5	0.3309066843150232	No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGCGTCGGA	5	0.3309066843150232	No Hit
GCTCAAGGGGATTGATTTGGTGTTCTGTATGTTCTTTTTA	5	0.3309066843150232	No Hit
GCTCAAGGGGAGTCCCTTCTTCTGAGAAGGGAAAACATTG	4	0.26472534745201853	No Hit
GCTCAAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGC	3	0.1985440105890139	No Hit
GCTCAAGGGGCGATAGGAGTGGCACCGTGGATCCCCCGGA	3	0.1985440105890139	No Hit
GCTCAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGG	3	0.1985440105890139	No Hit
GCTCAAGGGAGGTTGAAGTGGTTTGTCATATGCTTTGTTG	3	0.1985440105890139	No Hit
GCTCAAGGGGATCCAGTGACAGATACCATTAGATTCAAGA	3	0.1985440105890139	No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCCGAGTCGGA	2	0.13236267372600927	No Hit
GCTCAAGGGGAGTCTTTTGCCTCAGACGCTAGCTGTAGCT	2	0.13236267372600927	No Hit
GCTCAAGGGTGTCAGTGAGCTAACTATTCTTGTCCAGAAA	2	0.13236267372600927	No Hit
GCTCAAGGGGATCCAGTGACAGATACCATTAGATTCCCGA	2	0.13236267372600927	No Hit
GCTCAAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGA	2	0.13236267372600927	No Hit
GCTCAAGGGGTTCTTTTTTCTTTCTTTTTTTTTTCCTTTT	2	0.13236267372600927	No Hit
GCTCAAGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGC	2	0.13236267372600927	No Hit
GCTCAAGGGCACGCAGATCGACTTTTTTTTTTTTTTTTTT	2	0.13236267372600927	No Hit
GCTCAAGGGGGCTCTAGCCATCCTCAGCATCCTGTAGTAT	2	0.13236267372600927	No Hit
GCTCAAGGGAGCAAATGCTGAACCGTGTGTCCTTCCTTTG	2	0.13236267372600927	No Hit
GCTCAAGGGAGAAAAAAATGTTTGTTTTTGGTTCTCTGTG	2	0.13236267372600927	No Hit
GCTCAAGGGGGGGCTGGACAGATGGCTCCGGGGTTACGAG	2	0.13236267372600927	No Hit
GCTCAAGGGAATTGACGGGTACGCACCGTTCCGAGTCGGC	2	0.13236267372600927	No Hit
GCTCAAGGGTGCAGAATTCAGTTGAAGTTGACATTCTTTT	2	0.13236267372600927	No Hit
GCTCAAGGGGCCGAATAGCCTCTCCACCCACGCCGCCCGA	2	0.13236267372600927	No Hit
GCTCAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTTAA	2	0.13236267372600927	No Hit
GCTCAAGGGGGTAGGAACCTGAGGGAGCTAACTGAAGCCT	2	0.13236267372600927	No Hit
GCTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCCGA	2	0.13236267372600927	No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCCGC	2	0.13236267372600927	No Hit
GCTCAAGGGGGAAAAAACCTTTAATAGTGAATAATTAACA	2	0.13236267372600927	No Hit
GCTCAAGGGAATTGACGGGTACGCACCGTTCTGCGTCCGA	2	0.13236267372600927	No Hit
GCTCAAGGGAAGCATGGTTCAACGCAGAGTCGACTTTTTT	2	0.13236267372600927	No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC	2	0.13236267372600927	No Hit
GCTCAAGGGTCACAGAGTTTTTTTTTTTTTTTTTTTTTTT	2	0.13236267372600927	No Hit
GCTCAAGGGAATTGACGGGTACGCCCAGTTCTGAGTCGGA	2	0.13236267372600927	No Hit
GCTCAAGGGGCTCTCTCCGCCATCTTGGCGCCTGTGGAGG	2	0.13236267372600927	No Hit
GCTCAAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA	2	0.13236267372600927	No Hit
GCTCAAGGGATTTAGCTGGTGCTTCAAGCCACCGTTTGAA	2	0.13236267372600927	No Hit
GCTCAAGGGGTTCAGACCAAACGTCGGGCCCCGGAGAAGA	2	0.13236267372600927	No Hit
GCTCAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	2	0.13236267372600927	No Hit
GCTCAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCT	2	0.13236267372600927	No Hit
GCTCAAGGGAAGCAGGGATGAGATGGACTTAAATGAATTG	2	0.13236267372600927	No Hit
GCTCAAGGGGGATGGTTTGAGTTAGGATCTTTTTGCATTT	2	0.13236267372600927	No Hit
GCTCAAGGGGAATTTGAGTACCCTAAGTGCCTGGTATATG	2	0.13236267372600927	No Hit
GCTCAAGGGCTCGTCTTAATCAGCAACATCTTTTGATAAA	2	0.13236267372600927	No Hit
GCTCAAGGGGGGAATTCCCTCGATTCTGTTTTGCTACCCG	2	0.13236267372600927	No Hit
GCTCAAAGGAATTGACGGGTACGCACAGTTCTGCGTCGGA	2	0.13236267372600927	No Hit
GCTCAAGGGAGAAAGATGGGGTCTTAAGTTTTGATTGGAT	2	0.13236267372600927	No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACC	2	0.13236267372600927	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	2	0.13236267372600927	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGGAA	25	1.02090984E-4	34.931038	5
CAAGGGG	50	4.1836756E-11	34.931038	4
TCAAGGG	120	0.0	34.931034	3
AAGGGGA	20	0.0017258695	34.931034	5
AGGGAAT	20	0.0017258695	34.931034	6
CAAGGGA	45	8.62201E-10	34.931034	4
GCTCAAG	130	0.0	34.931034	1
CTCAAGG	120	0.0	34.931034	2
GGGTACG	25	1.2707495E-4	33.766666	17
GACGGGT	35	4.353933E-7	33.766666	14
CGGGTAC	30	7.5699445E-6	33.766666	16
TACGCAC	25	1.2707495E-4	33.766666	20
ACGGGTA	30	7.5699445E-6	33.766666	15
GGAATTG	35	4.353933E-7	33.766666	8
GGTACGC	25	1.2707495E-4	33.766666	18
TGACGGG	35	4.353933E-7	33.766666	13
GTACGCA	25	1.2707495E-4	33.766666	19
CTGCGTC	20	0.0020642441	33.766663	31
TCTGCGT	20	0.0020642441	33.766663	30
TTGACGG	40	2.4114343E-8	33.766663	12
>>END_MODULE