FastQCFastQC Report
Fri 17 Jun 2016
SRR1525892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length40
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGACGCATGGTCTCAACCCCGATCGCCTTTTTT11.0526315789473684No Hit
GCTCAAGGGAGAGTGGTCCCACCGTCGCCTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGGGGCTGGTCCGATGGCCCAGTGGGTAAGAGC11.0526315789473684No Hit
GCTCAAGGGACCATGGTATCCCCAGAGCCGACTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGGGCGGCCTTGGCACCGCCCCTGTGGCCCGCG11.0526315789473684No Hit
GCTCAAGGGAAGTATCCCCCCCGCGTCGACTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGTGGTACCCCGGCGCGTCGCCTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGAATGCAGCGCCCACCGATCTCTTCTTTTTTT11.0526315789473684No Hit
GCTCAAGGGAAGCATGGCCTCAACCCACAGCCTCTTTTTT11.0526315789473684No Hit
GCTCAAGGGACGCTGGATCAACCCAGAGTCGACTTTTTTT11.0526315789473684No Hit
GCTCAAGGGGCGCAGTTCCGGTGAACCTCCCGGCGTTTGT11.0526315789473684No Hit
GCTCAAGGGAATCATCGTTCCTCTAACCACAGCCTCTTTT11.0526315789473684No Hit
GCTCAAGGGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGACATGGTGGCTCACCCCCCTCTGTACCACGA11.0526315789473684No Hit
GCTCAAGGGGGGCTGGCGCGACGGCTCAGAGGTTAAGCGC11.0526315789473684No Hit
GCTCAAGGGGAGATTTCTGCCGTTGCGCCTGCCGTTGGCT11.0526315789473684No Hit
GCTCAAGGGGCCGAGTCGACTTTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGCCTCTCACGAGACGACGTTAGGAATGGGAAT11.0526315789473684No Hit
GCTCAAGGGATTTGTTGGCTAGCTGCCTTACTTTGAGATC11.0526315789473684No Hit
GCTCAAGGGAGTCGACTTCTTTTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGGGGCTGGTTGATCCCCCCAGTACCATCTGCT11.0526315789473684No Hit
GCTCAAGGGGGGGCCGCACAGATGGCTCAGCACTTAAGAG11.0526315789473684No Hit
GCTCAAGGGTGAACCCGCCAGTCGACTTGTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGGATTCTGAATATCAAATCCATAGGTTATACT11.0526315789473684No Hit
GCTCAAGGGACTCTCAAGCGACAAAGTTAGGAATGGGAAT11.0526315789473684No Hit
GCTCAAGGGCATGTAAGTCCACTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGCTCGCAGAGTCGTTTTTTTTTTTTTCCCTTT11.0526315789473684No Hit
GCTCAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGTGGTCAACAGAGCGACTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGCATTGACGGGTCCCCCCCGTTCTGCGTCGGC11.0526315789473684No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGCCGTACGAGC11.0526315789473684No Hit
GCTCAAGGGCAAGCTCCCCTCCCCCCCCTAGACATACATG11.0526315789473684No Hit
GCTCAACCCTCCACCCCCCCCCAACCCCCCCCCACCCCCC11.0526315789473684No Hit
GCTCAAGGGGGTCCTAGCGTTGTATTCTGGGTCGCGTGTC11.0526315789473684No Hit
GCTCAAGGGACTTTTTCTTTTTTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGAAGTGGTACCACCCCGCCCCGCCCTTTTTTT11.0526315789473684No Hit
GCTCAAGGGGGGAAAGCACCCTGGGTATGAGAGGTTCACC11.0526315789473684No Hit
GCTCAAGGGCTCTCCCCGCCCCCCCCACCCCCCCCCCTCC11.0526315789473684No Hit
GCTCAAGGGCCGCTGTATCACCCCCGAGTCGCCTTTTTTT11.0526315789473684No Hit
GCTCAAGGGATGCACCCCGCGTCCTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGAAGCATGGTCTCAACCCCGTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGGGGTTCAGCCTGGGGCTCACACCTCACAACT11.0526315789473684No Hit
GCTCAAGGGCCCGCTCCAATCCCCCCCTTAGAAATCCATG11.0526315789473684No Hit
GCTCAAAGGCATTGATGGGTACCCACAGTTCTGAGTCGGA11.0526315789473684No Hit
GCTCAAGGGGGACTTTTTTTTTCTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGAATTGACGGGTACCCACCCTCCTGAGTCGGA11.0526315789473684No Hit
GCTCAAGGGGGTTCTTCAAACCTTGGGTGCCCCGGCGTGG11.0526315789473684No Hit
GCTCAAGGGCCTTCTTTTTTTTTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGGAGATTACACGGGGGCTAATACTGGGTCCGG11.0526315789473684No Hit
GCTCAAGGGCGCGTGGCCAACCCAGACCCACTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGTACAGTCTTTTTTTTTTTTTTTTTTCCCCTT11.0526315789473684No Hit
GCTCAAGGGAAGCATGGTCTCAACCCAAAATAAAAAAAAA11.0526315789473684No Hit
GCTCAAGGGGGGCCTTTTTATCTTCAAGCCGCTGCCGGCC11.0526315789473684No Hit
GCTCAAGGGCCAGTCGCTTTTTTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGCCCATTATCAAGCAGGCAAACCCGCCGCTAT11.0526315789473684No Hit
GCTCAAGGGGGACGTGAGGCGACACCATGCCGCCCACCCC11.0526315789473684No Hit
GCTCAAGGGAAGCAGTGCCCCCCGCTCGCCTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGCGGTAACCCCGAGCGCCTTTCTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGTGGACCGCGTCGTTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGCTCGTCTTAATCAGCAAACAAAAAAAAAAAA11.0526315789473684No Hit
GCTCAAGGGAATACGCAGAGTCGATTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAAGGTCCAGATCGACTTTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGCATTGACGGGTCCCCCCCGCTCCCCGCCCGC11.0526315789473684No Hit
GCTCAAGGGAACTTCCAACTCCATTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAACGTCCACACCACACAACATCTTATCTCTTCTTCT11.0526315789473684No Hit
GCTCAAAGGACTTGACGGGTACCCACACCTCCCCGCCGCA11.0526315789473684No Hit
GCTCAAGGGGGGTAAGAGCCCCCGACTGCTCTTCCAAAGG11.0526315789473684No Hit
GCTCAAGGGAATTGACGGGTACCCCCAGTTCTGAGCCGGA11.0526315789473684No Hit
GCTCAAGGGGTGTGTGTGGGTATGTACTTCCTGTCTGGAT11.0526315789473684No Hit
GCTCAAGGGAAGCATGGTATCAACCCAAAAAAAAAAAAAA11.0526315789473684No Hit
GCTCAAGGGGACTTCTCCTTGAAAAAAAAACCCCTTGAGT11.0526315789473684No Hit
GCTCAAGGGCAGTGACCTTTTTTTCCTTGTGATTTGTGCC11.0526315789473684No Hit
GCTCAAGGGAGAATTTAATAGCAGCCCCTCCCACCCAACC11.0526315789473684No Hit
GCTCAAGGGAATTGACGGGTACCCCCCGTTCCCAGCCGGA11.0526315789473684No Hit
GCTCAAGGGTTCCACAGCCTTTTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGAGTCCTTTTTTTTTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGACAGTGTTCAACCCAGAGCCGCCTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTCTTTT11.0526315789473684No Hit
GCTCAAGGGGGACACCATCCCAGAAAGGAGCCCGTCTGCC11.0526315789473684No Hit
GCTCAAGGGACGCAGAGCGACTTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGGAACCTCTTTTCTTCACCACCTCCTCCCCCC11.0526315789473684No Hit
GCTCAAGGGAAATGAAAGGATCTGACTGCAAAAAAAAAAA11.0526315789473684No Hit
GCTCAAGGGGGGACTTTTTATCTTCAACCCGATGACGGAA11.0526315789473684No Hit
GCTCAAGGCCATGGATCAACCCGAGCCGCCTTTTTTTTTT11.0526315789473684No Hit
GCTCAAAGGAATTGACGGGTACCCCCAGTTCTGAGTCGGA11.0526315789473684No Hit
GCTCAAGGGAAGCAGTGGTCCCAACCCCCCAACAAAAACC11.0526315789473684No Hit
GCTCAAGGGTATCAACGCCGACCCACCTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGGGGCTGGTGCGATGGCTCCGTGGGTCCGCGC11.0526315789473684No Hit
GCTCAAGGGATCACGAGTCGACTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGAACTGCAATGATCTGCCTCCAAACAACACAA11.0526315789473684No Hit
GCTCAAGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GCTCAAGGGAATTGCCGGGTACCCCCACTTCCCAGTCCGA11.0526315789473684No Hit
GCTCAAGGGCCCTTAACCCAGACTCCATTTTTTTTTCTTT11.0526315789473684No Hit
GCTCAAGGGTGCTCTGCGGGCAGAGTTTGCGCCAGCGGCA11.0526315789473684No Hit
GCTCAAGGGGCTGCTCCAGGGTCAGTCGATTTTTTTTTTT11.0526315789473684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGA50.034.011
ACGCTGG50.034.010
TCAAGGG50.034.03
TCGACTT50.034.029
CCCAGAG50.034.022
CAACCCA50.034.019
TTTTTTT50.034.034
GCTGGAT50.034.012
CCAGAGT50.034.023
GGATCAA50.034.015
GTCGACT50.034.028
AACCCAG50.034.020
TCAACCC50.034.018
AAGGGAC50.034.05
ACTTTTT50.034.032
CAGAGTC50.034.024
GAGTCGA50.034.026
AGGGACG50.034.06
GACGCTG50.034.09
AGAGTCG50.034.025
Produced by FastQC (version 0.11.5)