FastQCFastQC Report
Fri 17 Jun 2016
SRR1525890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28902
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT7142.470417272161096No Hit
AACCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC5141.7784236385025256No Hit
AACCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1830.6331741747975919No Hit
AACCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA1450.5016953844024635No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1320.4567157982146564No Hit
AACCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1180.4082762438585565No Hit
AACCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT600.2075980900975711No Hit
AACCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC480.1660784720780569No Hit
AACCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC450.15569856757317835No Hit
AACCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAAAAA320.11071898138537124No Hit
AACCTAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC310.10725901321707841No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG310.10725901321707841No Hit
AACCTAGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC290.10033907688049269No Hit
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT290.10033907688049269No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCC203.8576685E-450.6578541
GATTGAC203.8576685E-450.6578541
CATTGAA354.5542038E-850.65784541
TTGTCCA203.8914778E-450.56866537
TGTCCAA203.8914778E-450.56866538
CACCCGA203.8914778E-450.56866540
TCCAATC203.8914778E-450.56866540
CGCATTG354.6182322E-850.56866539
GCATTGA354.6182322E-850.56866540
CGGATCC150.00826433250.127413
TCTCGGC150.00826433250.127415
TTCTCGG150.00826433250.127414
CCATCCT150.00826433250.127414
TCCCATG150.00832163350.0400748
CCATGCT150.00832163350.0400750
ACTCCGC150.00832163350.0400743
CACTCCG150.00832163350.0400742
AGTCCCG150.00832163350.0400711
ACGGATC150.00832163350.0400712
CTCCGCC150.00832163350.0400744