##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525890_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28902
Sequences flagged as poor quality	0
Sequence length	56
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.10501003390769	38.0	35.0	39.0	31.0	40.0
2	35.183101515466056	38.0	35.0	40.0	27.0	40.0
3	34.51200608954397	38.0	34.0	40.0	23.0	40.0
4	34.84364403847485	38.0	35.0	40.0	24.0	40.0
5	34.68746107535811	38.0	35.0	40.0	24.0	40.0
6	33.61691232440661	38.0	33.0	39.0	17.0	40.0
7	34.55297211265656	38.0	35.0	39.0	25.0	40.0
8	34.64286208566881	38.0	35.0	39.0	25.0	40.0
9	34.70822088436786	38.0	35.0	39.0	25.0	40.0
10	33.90820704449519	38.0	33.0	39.0	20.0	40.0
11	33.68656148363435	38.0	33.0	39.0	21.0	40.0
12	32.69867137222337	38.0	30.0	39.0	17.0	40.0
13	31.906477060411046	37.0	29.0	39.0	14.0	40.0
14	32.88101169469241	38.0	31.0	39.0	18.0	40.0
15	31.252889073420526	36.0	28.0	39.0	11.0	40.0
16	32.37848591792955	37.0	30.0	39.0	17.0	40.0
17	31.192685627292228	36.0	27.0	39.0	13.0	40.0
18	30.802020621410282	35.0	26.0	39.0	12.0	40.0
19	32.06003044771988	36.0	29.0	39.0	17.0	40.0
20	32.5068853366549	37.0	30.0	39.0	19.0	40.0
21	32.99730122482873	38.0	31.0	39.0	18.0	40.0
22	32.3135423154107	38.0	30.0	39.0	15.0	40.0
23	33.00671233824649	38.0	31.0	39.0	18.0	40.0
24	32.31734828039582	37.0	30.0	39.0	15.0	40.0
25	32.82008165524877	38.0	31.0	39.0	18.0	40.0
26	33.03944363711854	38.0	32.0	39.0	18.0	40.0
27	33.223375544944986	38.0	33.0	39.0	18.0	40.0
28	33.254169261642794	38.0	33.0	39.0	19.0	40.0
29	32.77797384264065	38.0	32.0	39.0	17.0	40.0
30	32.62608124005259	38.0	31.0	39.0	16.0	40.0
31	33.17853435748391	38.0	33.0	39.0	17.0	40.0
32	33.14549166147671	38.0	33.0	39.0	17.0	40.0
33	32.79904504878555	37.0	33.0	39.0	15.0	40.0
34	32.548162756902634	37.0	32.0	39.0	13.0	40.0
35	32.64839803473808	38.0	32.0	39.0	11.0	40.0
36	31.58096325513805	36.0	30.0	39.0	4.0	40.0
37	31.437236177427167	36.0	30.0	39.0	4.0	40.0
38	30.962493945055705	36.0	30.0	38.0	4.0	40.0
39	29.827347588402187	35.0	27.0	38.0	4.0	40.0
40	27.54062002629576	33.0	18.0	38.0	4.0	40.0
41	25.356272922289115	31.0	12.0	37.0	4.0	39.0
42	26.340391668396652	31.0	18.0	37.0	4.0	39.0
43	26.64902082900837	32.0	18.0	37.0	4.0	39.0
44	25.86094387931631	31.0	14.0	37.0	4.0	39.0
45	26.64379627707425	32.0	19.0	37.0	4.0	39.0
46	24.45121444882707	29.0	11.0	36.0	4.0	39.0
47	23.62334786519964	28.0	7.0	35.0	4.0	39.0
48	24.235900629714205	29.0	10.0	36.0	4.0	39.0
49	23.21500242197772	28.0	4.0	35.0	4.0	39.0
50	23.99692062833022	29.0	4.0	36.0	4.0	39.0
51	24.243720157774547	30.0	4.0	36.0	4.0	39.0
52	24.09580651858003	30.0	4.0	36.0	4.0	39.0
53	24.026987751712685	30.0	4.0	36.0	4.0	39.0
54	23.690125250847693	30.0	4.0	36.0	4.0	39.0
55	23.614317348280395	30.0	4.0	36.0	4.0	39.0
56	22.9146771849699	29.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	4.368917157795622
1	2	5.88431343196136
1	3	6.640437985724198
1	4	6.486016341420445
1	5	6.445001417364658
1	6	7.44884220871991
1	7	6.330970621756812
1	8	6.087366759361075
1	9	6.109129786935306
1	10	6.987078880431781
1	11	2.8736110440808034
1	12	-3.081996575610191
1	13	-14.532348843019337
1	14	-2.580888684779975
1	15	2.8510225817149255
1	16	0.3914103366305923
1	17	-2.795535336720235
1	18	-2.3828513854593467
1	19	1.5145393629271773
1	20	1.0735443156538729
1	21	6.537446761587255
1	22	7.555551246449987
1	23	6.426530922143403
1	24	7.919739946293493
1	25	6.94479985245658
1	26	6.767110192733554
1	27	6.155319607914965
1	28	6.30216815812015
1	29	6.654723600924655
1	30	7.052994730211772
1	31	5.8368529678138685
1	32	5.243873958702483
1	33	6.288584001297224
1	34	6.132372128952564
1	35	5.906370033725981
1	36	4.721047562927382
1	37	4.410891044770903
1	38	5.545445093269503
1	39	6.3865178333940555
1	40	-8.594031750087073
1	41	4.063271766107039
1	42	1.638679194901627
1	43	-5.431862799854674
1	44	-12.040532805664215
1	45	1.179390830258022
1	46	-2.9191363819641225
1	47	-6.832493505644948
1	48	-1.7787351177937119
1	49	-11.01563351782558
1	50	-0.21937681124142117
1	51	5.143147173401271
1	52	4.955057630377162
1	53	4.964367075703805
1	54	5.629271221712667
1	55	4.94849272373548
1	56	8.903302901851621
2	1	5.258245221036724
2	2	6.482139518917883
2	3	7.4675130845384246
2	4	7.299257448139812
2	5	6.572471773095884
2	6	8.507142603976831
2	7	7.120496313456414
2	8	7.45001498070495
2	9	7.272173265196173
2	10	7.8694899476254605
2	11	7.222425273329819
2	12	4.761876942176368
2	13	6.458757876348255
2	14	7.69776744170224
2	15	9.614856573809789
2	16	6.936864882085139
2	17	9.088852015058418
2	18	7.389875887267927
2	19	8.01058679375722
2	20	7.584413880871264
2	21	8.643177986883693
2	22	9.023930693090307
2	23	8.603408392499137
2	24	9.45432492653065
2	25	8.599938192377532
2	26	7.917307821192054
2	27	7.77488482530627
2	28	7.679638513851376
2	29	8.554921229383154
2	30	8.751611331002287
2	31	6.899105932240744
2	32	7.727075539730155
2	33	7.374552380743864
2	34	7.9495658048418925
2	35	7.668227741235864
2	36	9.629150329725796
2	37	9.36049578785391
2	38	9.771729678249738
2	39	9.508059335370344
2	40	10.049248882323997
2	41	13.436789552668301
2	42	11.992434135613092
2	43	11.828018623070225
2	44	2.136344664691517
2	45	12.739667510099917
2	46	11.978096819616905
2	47	8.940233767082326
2	48	11.819090969162811
2	49	5.200769644229755
2	50	11.476275362671625
2	51	13.439589861148306
2	52	13.265334310219057
2	53	13.6046832812374
2	54	12.624330510250218
2	55	14.189599443103074
2	56	13.826227803037394
3	1	4.740129279007739
3	2	6.217646765294695
3	3	7.371498591784807
3	4	6.773895129299234
3	5	7.001819599182838
3	6	7.9564179662956676
3	7	6.906728197514383
3	8	6.740775850270168
3	9	6.796629786935306
3	10	7.845033425886328
3	11	7.125504983474741
3	12	8.160427666814055
3	13	9.158939035768544
3	14	7.640702224310935
3	15	3.856704399896742
3	16	7.874364882085139
3	17	9.00370708752219
3	18	10.244042553934591
3	19	8.527796938684752
3	20	7.948544315653873
3	21	8.531764943405435
3	22	9.057445185843925
3	23	8.203046073658559
3	24	9.048527825081376
3	25	7.679648337305064
3	26	7.763322313945672
3	27	6.613652941248301
3	28	7.879819673271665
3	29	7.025935722136772
3	30	7.725343215060256
3	31	7.518671149632048
3	32	7.23251032233885
3	33	7.1408567285699505
3	34	7.677826674407111
3	35	7.13743063978659
3	36	8.732411199291015
3	37	8.251800135679996
3	38	9.04165721448162
3	39	9.286139045515267
3	40	11.993089462034138
3	41	14.112514190349465
3	42	12.941709497931928
3	43	12.816243260751389
3	44	13.069315679184268
3	45	12.690754466621659
3	46	14.029727254399514
3	47	13.023567100415661
3	48	14.450431548872952
3	49	14.69080587611381
3	50	13.593123188758579
3	51	13.440495658249752
3	52	14.237254600074134
3	53	13.149973136309868
3	54	14.078134858076304
3	55	14.493947269190027
3	56	12.401408962457683
4	1	5.14999322458597
4	2	6.522068534002173
4	3	7.285614238043308
4	4	7.09872506127202
4	5	6.876819599182838
4	6	8.45301660575145
4	7	6.561490102276288
4	8	6.846643197208941
4	9	7.124435909384282
4	10	7.680492609559799
4	11	7.773889337216239
4	12	7.7250535171541905
4	13	8.885554682027045
4	14	8.930243040637464
4	15	8.766143175406945
4	16	8.681762841268814
4	17	10.473945182760286
4	18	10.471508540329154
4	19	8.80118129242625
4	20	8.772523907490605
4	21	8.64273433116054
4	22	8.685421376320114
4	23	8.566141311753793
4	24	8.796827144809267
4	25	8.278287793087376
4	26	7.231859728911658
4	27	7.364503281384355
4	28	7.259496544019964
4	29	7.185799667715003
4	30	7.978319405536446
4	31	6.268671149632048
4	32	6.949347057032728
4	33	6.929122034692398
4	34	7.580887898896908
4	35	7.133178939106319
4	36	7.250268342148161
4	37	7.966085849965712
4	38	8.362235445774143
4	39	8.753400950277172
4	40	-0.45163842912232255
4	41	13.388874734567153
4	42	12.04034895371424
4	43	11.749491560071117
4	44	13.578669420680871
4	45	12.54534630335635
4	46	14.839676233991351
4	47	15.154519481368041
4	48	14.542693453634858
4	49	15.614700433936942
4	50	15.239806862227969
4	51	14.042961644644315
4	52	14.132237593271409
4	53	14.7426602111398
4	54	15.12787975603549
4	55	14.106192167149214
4	56	14.110167465859039
5	1	4.401240390118851
5	2	5.467646765294695
5	3	5.87427636956258
5	4	5.97945068485479
5	5	5.654597376960616
5	6	5.84530685518456
5	7	4.967839308625493
5	8	4.417164739159055
5	9	4.792463120268636
5	10	5.426977870330774
5	11	6.118560539030298
5	12	6.115983222369611
5	13	7.029772369101874
5	14	6.80598000208871
5	15	8.685871066563408
5	16	6.6590871043073605
5	17	8.578707087522186
5	18	9.434320331712371
5	19	7.323630272018086
5	20	6.19021098232054
5	21	7.280376054516545
5	22	7.272722963621703
5	23	5.594712740325225
5	24	6.915194491748043
5	25	6.0129816706384
5	26	5.454988980612342
5	27	5.294208496803851
5	28	5.195097451049438
5	29	5.814824611025664
5	30	5.521176548393591
5	31	4.290893371854274
5	32	4.379732544561076
5	33	3.1880789507921747
5	34	4.550048896629331
5	35	4.640208417564363
5	36	6.107411199291015
5	37	5.851800135679998
5	38	6.527768325592735
5	39	7.248639045515272
5	40	7.84586723981192
5	41	11.209736412571687
5	42	9.241709497931932
5	43	9.145409927418054
5	44	9.938760123628715
5	45	10.543532244399433
5	46	11.317227254399516
5	47	12.151344878193434
5	48	11.793487104428511
5	49	12.38386143166937
5	50	11.325067633203023
5	51	8.561328991583089
5	52	8.291421266740798
5	53	10.036084247420977
5	54	10.07119041363186
5	55	10.027280602523362
5	56	9.530575629124346
6	1	3.823462612341075
6	2	6.043404341052266
6	3	6.276801622087838
6	4	6.417834523238625
6	5	6.240455962819205
6	6	7.426114935992636
6	7	5.330970621756812
6	8	4.996457668451988
6	9	5.7909479687534855
6	10	5.896169789522695
6	11	6.759974680444444
6	12	7.622548878935266
6	13	8.467651156980661
6	14	7.169111315220029
6	15	9.055568036260375
6	16	7.255046700266959
6	17	8.431737390552488
6	18	8.003512250904294
6	19	7.287266635654454
6	20	7.664453406562966
6	21	7.741992216132708
6	22	7.532823973722714
6	23	6.65380364941613
6	24	7.078830855384407
6	25	6.422072579729306
6	26	5.471655647279007
6	27	5.268955971551332
6	28	6.30216815812015
6	29	6.700178146379201
6	30	6.7802674574844986
6	31	4.973216604177502
6	32	3.834783049611577
6	33	5.061311274024497
6	34	3.859644856225291
6	35	3.9518245791805278
6	36	5.6528657447455615
6	37	5.160891044770903
6	38	5.999990547814956
6	39	5.522881469757696
6	40	2.201422795367474
6	41	10.08599903883431
6	42	8.956861013083444
6	43	9.545409927418053
6	44	9.232194467063056
6	45	8.247572648439842
6	46	10.87631816349042
6	47	10.917506494355052
6	48	10.880355791297198
6	49	12.507093754901693
6	50	9.894259552394942
6	51	10.66587444612854
6	52	10.682330357649889
6	53	10.078003439340169
6	54	9.33381667625812
6	55	10.19849272373548
6	56	8.8351210836698
7	1	4.187098975977442
7	2	5.861586159234086
7	3	6.867710712996924
7	4	6.326925432329531
7	5	6.399546871910111
7	6	7.90338766326537
7	7	5.876425167211352
7	8	6.314639486633801
7	9	5.995493423298939
7	10	7.077987971340875
7	11	7.055429225898983
7	12	7.668003424389813
7	13	8.717651156980661
7	14	8.373656769765482
7	15	8.805568036260375
7	16	8.50504670026696
7	17	9.318101026916128
7	18	9.77623952363156
7	19	8.12817572656354
7	20	7.982635224744779
7	21	7.537446761587255
7	22	8.555551246449987
7	23	7.7674400130524965
7	24	8.46519449174804
7	25	7.899345307002033
7	26	7.085292010915374
7	27	6.382592335187692
7	28	5.80216815812015
7	29	6.177450873651928
7	30	6.802994730211772
7	31	5.132307513268415
7	32	5.60751032233885
7	33	5.879493092206317
7	34	5.496008492588931
7	35	5.656370033725981
7	36	6.539229381109195
7	37	7.52452740840727
7	38	7.818172365996777
7	39	7.704699651575876
7	40	7.565059159003841
7	41	10.97236267519795
7	42	11.115951922174357
7	43	10.02268265469078
7	44	10.982194467063056
7	45	10.452118102985292
7	46	12.671772708944967
7	47	12.985688312536873
7	48	12.539446700388108
7	49	12.80254830035624
7	50	11.530623188758579
7	51	10.370419900673998
7	52	10.568693994013525
7	53	10.850730712067442
7	54	11.538362130803577
7	55	11.312129087371844
7	56	10.948757447306164
8	1	4.779018167896631
8	2	6.156535654183585
8	3	7.207609702895915
8	4	6.935006240410345
8	5	6.810152932516168
8	6	8.089751299629
8	7	6.434505975292161
8	8	6.483831405825725
8	9	6.792463120268636
8	10	7.271422314775215
8	11	8.02967165014141
8	12	8.293761000147388
8	13	9.518661257990761
8	14	7.783757779866491
8	15	9.108093288785632
8	16	8.614642659862916
8	17	10.512040420855524
8	18	10.234320331712368
8	19	8.834741383129199
8	20	8.390210982320543
8	21	8.435931610072103
8	22	9.739389630288372
8	23	8.194712740325226
8	24	9.426305602859149
8	25	7.835203892860623
8	26	7.099433425056787
8	27	6.9386529412482965
8	28	7.350653006604997
8	29	6.748157944358994
8	30	6.698954326171368
8	31	6.046448927409827
8	32	6.335288100116625
8	33	6.099190061903286
8	34	5.372271118851554
8	35	6.306875084231034
8	36	6.862966754846568
8	37	6.940689024568886
8	38	7.038879436703848
8	39	8.115305712181932
8	40	2.7792005731452534
8	41	12.009736412571684
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116	44	0.8925138773824663
116	45	5.863051763918957
116	46	2.1496596868319457
116	47	-5.77598244913389
116	48	0.7550486659900706
116	49	-1.4437661959582613
116	50	3.199542107677498
116	51	4.9343019645560595
116	52	5.496826672146206
116	53	5.894342505679234
116	54	5.596715939157384
116	55	4.575028350271108
116	56	6.698143196691916
117	1	3.823462612341075
117	2	5.13431343196136
117	3	5.5231652584514705
117	4	5.530561795965902
117	5	5.516819599182838
117	6	6.929751299629004
117	7	5.670061530847718
117	8	5.608275850270168
117	9	5.5791297869353045
117	10	5.862533425886326
117	11	0.8563383168080776
117	12	-7.492905666519281
117	13	-6.63689429756479
117	14	-0.4117977756890632
117	15	-7.740795600103258
117	16	-8.24313511791486
117	17	0.005373754188855884
117	18	-1.8501241127320718
117	19	0.043630272018088334
117	20	-0.9964556843461274
117	21	5.049264943405433
117	22	4.50827851917726
117	23	3.5547127403252254
117	24	4.155194491748038
117	25	3.8529816706384032
117	26	5.92165564727901
117	27	5.205319607914962
117	28	4.643986339938326
117	29	5.4992690554701085
117	30	5.2411765483935895
117	31	4.5486711496320495
117	32	4.677510322338851
117	33	5.245856728569947
117	34	5.887826674407112
117	35	5.129097306453257
117	36	6.467411199291014
117	37	6.251800135679996
117	38	6.029990547814958
117	39	7.541972378848602
117	40	9.561422795367474
117	41	8.65418085701613
117	42	6.6950428312652654
117	43	-4.6545900725819465
117	44	0.3476490125176035
117	45	6.805754466621657
117	46	0.037227254399514464
117	47	-0.35976623291767496
117	48	3.1712648822062874
117	49	-2.678360790552855
117	50	3.72062318875858
117	51	8.361328991583086
117	52	9.091421266740799
117	53	7.43163980297653
117	54	8.075634858076302
117	55	8.693947269190026
117	56	12.05057562912435
118	1	3.490129279007739
118	2	4.88431343196136
118	3	5.913165258451471
118	4	5.065561795965898
118	5	5.626819599182838
118	6	6.9564179662956676
118	7	5.198394864181054
118	8	5.053275850270168
118	9	4.942463120268641
118	10	6.115866759219664
118	11	3.2296716501414124
118	12	-7.714572333185949
118	13	-3.945227630898124
118	14	-5.796797775689065
118	15	-7.622462266769926
118	16	-7.938135117914861
118	17	-0.2879595791444771
118	18	-3.6934574460654055
118	19	0.04863027201808734
118	20	0.32354431565387287
118	21	5.469264943405435
118	22	6.32827851917726
118	23	5.078046073658559
118	24	5.923527825081376
118	25	5.2629816706384
118	26	5.304988980612343
118	27	4.863652941248301
118	28	5.067319673271665
118	29	6.192602388803444
118	30	5.371176548393592
118	31	4.893671149632048
118	32	4.73251032233885
118	33	5.3908567285699505
118	34	5.136160007740447
118	35	5.679097306453254
118	36	7.899077865957679
118	37	6.751800135679996
118	38	7.666657214481621
118	39	8.848639045515267
118	40	10.90975612870081
118	41	8.987514190349462
118	42	7.275042831265264
118	43	-2.454590072581947
118	44	2.3609823458509354
118	45	6.524087799954991
118	46	1.5505605877328463
118	47	0.8569004337489936
118	48	1.387931548872956
118	49	0.1699725427804779
118	50	2.155623188758579
118	51	8.461328991583088
118	52	9.174754600074131
118	53	7.441639802976532
118	54	8.182301524742972
118	55	9.035613935856695
118	56	9.422242295791015
119	1	3.5642033530818154
119	2	4.912091209739138
119	3	5.755757851044066
119	4	5.135006240410341
119	5	5.321264043627281
119	6	7.067529077406782
119	7	5.656728197514383
119	8	5.5208684428627635
119	9	5.562833490639008
119	10	6.264014907367809
119	11	1.6000420205117791
119	12	-4.209942703556319
119	13	-2.125783186453681
119	14	-4.431057034948324
119	15	-6.062277081584739
119	16	-7.044616599396342
119	17	1.3787070875221872
119	18	-4.980494483102444
119	19	-0.11340676501895075
119	20	0.02724801935757526
119	21	5.11741309155358
119	22	5.939389630288375
119	23	5.328046073658559
119	24	5.270750047303597
119	25	5.087055744712476
119	26	6.2401741657975265
119	27	5.442356644952003
119	28	5.8543567103087035
119	29	5.933343129544184
119	30	6.195250622467668
119	31	4.3057081866690865
119	32	5.098251063079591
119	33	5.654745617458836
119	34	5.557456304036737
119	35	5.9661343434902925
119	36	6.625929717809534
119	37	6.696244580124439
119	38	7.527768325592735
119	39	7.4782686751449
119	40	10.164385758330436
119	41	5.431958634793908
119	42	4.367635423857855
119	43	-3.4916271096189853
119	44	-1.0464250615564694
119	45	1.3435322443994373
119	46	-2.3568468196745584
119	47	-4.485692158843602
119	48	-4.287994377052971
119	49	-5.223545975738039
119	50	-1.4230805149451236
119	51	2.368736398990496
119	52	2.8321620074815392
119	53	2.3397879511246806
119	54	1.886005228446674
119	55	2.049502824745584
119	56	5.760205258753977
120	1	3.920236805889459
120	2	5.295603754542007
120	3	5.743810419741791
120	4	5.142174699191706
120	5	5.7800454056344535
120	6	6.934912589951587
120	7	5.409416369557398
120	8	5.218598430915328
120	9	5.37525881919337
120	10	5.718017296854072
120	11	3.444725413582269
120	12	-1.5277443761967007
120	13	-1.882055587887372
120	14	-2.3976042273019686
120	15	2.290172141832226
120	16	-4.014748021140669
120	17	1.916341496124339
120	18	-1.0617370159578812
120	19	1.6333076913729272
120	20	0.5816088317829049
120	21	4.1950713950183385
120	22	5.796020454661132
120	23	5.220519191938127
120	24	5.13454933045772
120	25	4.6581429609609835
120	26	5.399075002117719
120	27	5.018222833721413
120	28	5.4194702109060735
120	29	5.214107765147524
120	30	6.411499129038752
120	31	4.849316310922369
120	32	4.873639354596914
120	33	4.798114793086079
120	34	5.2826653840845275
120	35	4.864581177420998
120	36	6.526766038000694
120	37	6.735671103421932
120	38	6.814506676847213
120	39	8.665843346590538
120	40	8.330455053431994
120	41	-2.4425933365322585
120	42	-0.35398942679925227
120	43	-2.0997513629045272
120	44	-13.746544535869493
120	45	-2.047148759184793
120	46	-5.15374048753597
120	47	-5.269443652272512
120	48	-3.641638343600164
120	49	-13.231909177649628
120	50	-1.945183262854325
120	51	2.9613289915830876
120	52	2.510776105450475
120	53	3.2561559320087916
120	54	2.7414413096892076
120	55	2.590721462738415
120	56	5.3563820807372515
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1327.0
5	139.0
6	104.0
7	60.0
8	104.0
9	78.0
10	68.0
11	56.0
12	61.0
13	74.0
14	86.0
15	131.0
16	166.0
17	191.0
18	245.0
19	304.0
20	352.0
21	463.0
22	517.0
23	604.0
24	736.0
25	798.0
26	861.0
27	930.0
28	975.0
29	932.0
30	1038.0
31	1273.0
32	1337.0
33	1611.0
34	1780.0
35	1988.0
36	2193.0
37	2345.0
38	2496.0
39	2479.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.36336585703411	0.5432150024219777	0.05535949069268562	0.03805964985122137
8	99.55020413812193	0.3771365303439208	0.041519618019514225	0.031139713514635667
9	98.80280959136361	0.38060966748555414	0.5951351164319574	0.22144562471886783
10	50.04497958618781	19.784097986298526	23.853020552210918	6.317901875302748
11	29.620787488755102	22.39983392152792	15.462597744100753	32.516780845616225
12	25.340806864576848	30.745277143450277	18.81876686734482	25.09514912462805
13	19.66299910040828	14.199709362673863	21.289184139505917	44.848107397411944
14	18.770327312988723	36.03556847277005	22.47941318939866	22.71469102484257
15	39.31849791376912	17.02712100139082	20.653685674547983	23.00069541029207
16	23.826177285318558	34.729916897506925	22.018698060941826	19.425207756232687
17	24.658245371171482	17.127530714656515	38.598373421007096	19.615850493164906
18	22.15071621341084	16.068092173552003	20.984706940696146	40.796484672341016
19	21.97494116018275	16.48553232728783	21.940329502976603	39.59919700955282
20	21.4352509264711	19.994458490631388	38.33339105738926	20.23689952550826
21	22.30026642676724	16.91290958790353	23.071866025397046	37.714957959932185
22	22.015777454847417	17.58009826309598	37.852051761123796	22.55207252093281
23	21.673240606186422	18.856826517196044	37.492215071621345	21.977717804996193
24	19.965397923875432	21.356401384083046	21.290657439446367	37.38754325259516
25	22.23721541761816	20.13009480312781	36.44038474846031	21.19230503079372
26	40.8570142951092	19.54241805406528	18.83977709321242	20.7607905576131
27	23.020735971198118	35.4242392771835	18.575829958112646	22.97919479350573
28	31.402671095425923	29.16407169054045	18.55234931838627	20.88090789564736
29	32.79512144416341	29.12580991649631	18.259935553168635	19.81913308617165
30	29.657320872274145	19.636552440290757	30.26306680512288	20.44305988231222
31	32.3518217362721	27.255112279851907	20.89201065707069	19.5010553268053
32	33.17645430321486	28.59120323909056	19.804824030176142	18.42751842751843
33	34.34577820876913	26.29355129181963	19.044815404862504	20.31585509454873
34	23.124913350894218	19.610425620407597	38.03549147372799	19.229169554970195
35	23.151804891567934	20.58477101087785	39.025150696320935	17.238273401233283
36	29.37532445059699	21.138605295033745	29.378785256964875	20.107284997404395
37	31.399175529150934	19.323102504590018	29.99618942044549	19.28153254581356
38	31.26191383911552	20.33757321595675	29.532457630055802	18.86805531487194
39	31.390957907500432	19.663952884115712	20.647843408972804	28.29724579941105
40	24.712162445049195	16.551531644686342	28.560463331239973	30.175842579024494
41	21.583715155118743	14.508061977545381	26.08163408065475	37.82658878668113
42	21.96898740763867	27.016338848995737	21.78860096437368	29.22607277899192
43	18.68688621299927	25.908318006732138	28.198632751500853	27.206163028767744
44	19.205781190285933	26.96730709099121	28.96848834381406	24.8584233749088
45	27.520360422803673	19.771270143822562	31.789984404782533	20.91838502859123
46	20.709525131907803	26.65231880033324	22.157039711191338	30.48111635656762
47	17.751582168440088	25.241671882606582	21.16280687113151	35.84393907782182
48	28.37164220437552	22.331071725283856	23.168789808917197	26.12849626142343
49	19.811910049972237	25.80163797890061	21.60258189894503	32.78387007218212
50	19.44415566413695	27.896177703850018	23.297640087327164	29.362026544685865
51	19.733555370524563	28.507493755203996	23.11615320566195	28.642797668609493
52	20.627165627165628	36.2023562023562	23.26749826749827	19.902979902979904
53	19.515155718942914	34.97606991745856	25.008670319761393	20.500104043837137
54	20.34127562168349	24.38872125689314	29.469704852079214	25.80029826934415
55	20.51335414498786	21.328477280610475	30.88796392646549	27.270204647936175
56	21.29459487570641	22.157889262559376	31.036993377942657	25.510522483791558
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.5
5	2.0
6	2.5
7	3.0
8	9.5
9	16.0
10	16.0
11	16.0
12	32.0
13	47.5
14	47.0
15	52.0
16	57.0
17	56.0
18	55.0
19	55.0
20	55.0
21	60.5
22	80.5
23	95.0
24	120.0
25	145.0
26	157.5
27	170.0
28	167.5
29	181.0
30	197.0
31	224.5
32	252.0
33	294.5
34	337.0
35	386.5
36	436.0
37	472.5
38	535.5
39	562.0
40	602.0
41	642.0
42	939.5
43	1237.0
44	1908.0
45	2579.0
46	2691.0
47	2362.0
48	1921.0
49	1770.5
50	1620.0
51	1576.0
52	1532.0
53	1600.0
54	1695.0
55	1722.0
56	1751.5
57	1781.0
58	1771.5
59	1762.0
60	1641.0
61	1520.0
62	1401.5
63	1181.5
64	1080.0
65	955.0
66	830.0
67	696.5
68	563.0
69	493.5
70	424.0
71	358.5
72	238.5
73	184.0
74	144.5
75	105.0
76	82.5
77	60.0
78	49.5
79	26.0
80	13.0
81	9.5
82	6.0
83	3.0
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0034599681682928513
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.49131547989758495
16	0.07611929970244274
17	0.02421977717804996
18	0.0
19	0.03459968168292851
20	0.10033907688049269
21	0.0034599681682928513
22	0.0
23	0.0
24	0.006919936336585703
25	0.0
26	0.03805964985122137
27	0.051899522524392776
28	0.0
29	0.1418586949000069
30	0.041519618019514225
31	0.0034599681682928513
32	0.017299840841464256
33	0.09687910871219985
34	0.1729984084146426
35	0.12455885405854267
36	0.02421977717804996
37	0.12109888589024981
38	0.16953844024634973
39	0.12801882222683553
40	0.8303923603902844
41	1.076050100339077
42	0.25949761262196386
43	0.2940972943048924
44	0.41173621202684935
45	0.16261850390976404
46	0.3252370078195281
47	0.49823541623417067
48	0.04843955435609992
49	0.29755726247318526
50	0.15569856757317835
51	0.26987751712684244
52	0.14531866306829977
53	0.23527783544391392
54	0.23873780361220676
55	0.24911770811708533
56	0.20413812192927824
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	28902.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.21278804235001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.33204526635386	67.18912186007888
2	6.541540160088324	9.84014947062487
3	1.766491857576594	3.9858833298733654
4	0.7544392308400036	2.2697391184001106
5	0.4324224859692704	1.6261850390976402
6	0.3358174625080504	1.515466057712269
7	0.1886098077100009	0.9930108643000485
8	0.11960621952341521	0.7196733790049131
9	0.10120526267365904	0.6850736973219846
>10	0.39562057226975805	4.71939658155145
>50	0.004600239212439047	0.2075980900975711
>100	0.01840095684975619	1.9998616012732684
>500	0.009200478424878094	4.248840910663622
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	714	2.470417272161096	No Hit
AACCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	514	1.7784236385025256	No Hit
AACCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	183	0.6331741747975919	No Hit
AACCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	145	0.5016953844024635	No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	132	0.4567157982146564	No Hit
AACCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	118	0.4082762438585565	No Hit
AACCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	60	0.2075980900975711	No Hit
AACCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	48	0.1660784720780569	No Hit
AACCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	45	0.15569856757317835	No Hit
AACCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAAAAA	32	0.11071898138537124	No Hit
AACCTAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC	31	0.10725901321707841	No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	31	0.10725901321707841	No Hit
AACCTAGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	29	0.10033907688049269	No Hit
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	29	0.10033907688049269	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.01729984084146426	0.0	0.0	0.0	0.0
7	0.01729984084146426	0.0	0.0	0.0	0.0
8	0.01729984084146426	0.0	0.0	0.0	0.0
9	0.01729984084146426	0.0	0.0	0.0	0.0
10	0.02421977717804996	0.0	0.0	0.0	0.0
11	0.031139713514635667	0.0	0.0	0.0	0.0
12	0.031139713514635667	0.0	0.0	0.0	0.0
13	0.03459968168292852	0.0	0.0	0.0	0.0
14	0.07611929970244274	0.0	0.0	0.0	0.0
15	0.07611929970244274	0.0	0.0	0.0	0.0
16	0.07957926787073559	0.0	0.0	0.0	0.0
17	0.0864992042073213	0.0	0.0	0.0	0.0
18	0.0864992042073213	0.0	0.0	0.0	0.0
19	0.0864992042073213	0.0	0.0	0.0	0.0
20	0.08995917237561414	0.0	0.0	0.0	0.0
21	0.093419140543907	0.0	0.0	0.0	0.0
22	0.09687910871219985	0.0	0.0	0.0	0.0
23	0.09687910871219985	0.0	0.0	0.0	0.0
24	0.1003390768804927	0.0	0.0	0.0	0.0
25	0.1003390768804927	0.0	0.0	0.0	0.0
26	0.10379904504878555	0.0	0.0	0.0	0.0
27	0.11071898138537126	0.0	0.0	0.0	0.0
28	0.12455885405854267	0.0	0.0	0.0	0.0
29	0.12455885405854267	0.0	0.0	0.0	0.0
30	0.12455885405854267	0.0	0.0	0.0	0.0
31	0.1418586949000069	0.0	0.0	0.0	0.0
32	0.1522385994048855	0.0	0.0	0.0	0.0
33	0.1522385994048855	0.0	0.0	0.0	0.0
34	0.1522385994048855	0.0	0.0	0.0	0.0
35	0.1522385994048855	0.0	0.0	0.0	0.0
36	0.15569856757317832	0.0	0.0	0.0	0.0
37	0.16607847207805687	0.0	0.0	0.0	0.0
38	0.16607847207805687	0.0	0.0	0.0	0.0
39	0.16607847207805687	0.0	0.0	0.0	0.0
40	0.16607847207805687	0.0	0.0	0.0	0.0
41	0.16607847207805687	0.0	0.0	0.0	0.0
42	0.17991834475122828	0.0	0.0	0.0	0.0
43	0.18337831291952114	0.0	0.0	0.0	0.0
44	0.2075980900975711	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATCC	20	3.8576685E-4	50.65785	41
GATTGAC	20	3.8576685E-4	50.65785	41
CATTGAA	35	4.5542038E-8	50.657845	41
TTGTCCA	20	3.8914778E-4	50.568665	37
TGTCCAA	20	3.8914778E-4	50.568665	38
CACCCGA	20	3.8914778E-4	50.568665	40
TCCAATC	20	3.8914778E-4	50.568665	40
CGCATTG	35	4.6182322E-8	50.568665	39
GCATTGA	35	4.6182322E-8	50.568665	40
CGGATCC	15	0.008264332	50.1274	13
TCTCGGC	15	0.008264332	50.1274	15
TTCTCGG	15	0.008264332	50.1274	14
CCATCCT	15	0.008264332	50.1274	14
TCCCATG	15	0.008321633	50.04007	48
CCATGCT	15	0.008321633	50.04007	50
ACTCCGC	15	0.008321633	50.04007	43
CACTCCG	15	0.008321633	50.04007	42
AGTCCCG	15	0.008321633	50.04007	11
ACGGATC	15	0.008321633	50.04007	12
CTCCGCC	15	0.008321633	50.04007	44
>>END_MODULE