FastQCFastQC Report
Fri 17 Jun 2016
SRR1525889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11381
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1611.414638432475178No Hit
AACCTAGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT600.5271944468851595No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG570.5008347245409015No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT500.43932870573763283No Hit
AACCTAGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT440.38660926104911697No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC390.34267639047535364No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC260.2284509269835691No Hit
AACCTAGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC230.20209120463931113No Hit
AACCTAGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT180.15815833406554783No Hit
AACCTAGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT180.15815833406554783No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG170.14937175995079519No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC170.14937175995079519No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT160.14058518583604254No Hit
AACCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT150.13179861172128987No Hit
AACCTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT130.11422546349178456No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCGCG120.10543888937703189No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC120.10543888937703189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTAG800.034.19111326
GCCAGTA800.034.19111325
CCCGTAG359.2800656E-734.19111326
TGCCAGT900.034.19111324
CTGCCAG900.034.19111323
GCCCGTA359.2800656E-734.19111325
CCTGCCC301.3066805E-534.1911122
CCTGCCA1050.034.1911122
AGGGGGG1500.034.0398256
AGGGGGC454.8949005E-934.0398256
AGTAGCA454.8949005E-934.03982528
GGGTACC1500.034.0398257
AGGGTAC1500.034.0398256
CTAGGGA3100.034.0398254
ATATGCT552.5465852E-1134.03982534
CATATGC552.5465852E-1134.03982533
GTAGCAT750.034.03982529
AGGGGCT200.002658995634.039826
AGGGGCA251.891247E-434.039826
AGGGGAG601.8189894E-1234.039826
Produced by FastQC (version 0.11.5)