FastQCFastQC Report
Fri 17 Jun 2016
SRR1525887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016
Sequences flagged as poor quality0
Sequence length40
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG242.3622047244094486No Hit
AACCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT232.263779527559055No Hit
AACCTAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT70.6889763779527559No Hit
AACCTAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG40.39370078740157477No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT30.2952755905511811No Hit
AACCTAGGGAGACACTGATGGACTTTGTGACTATGGGCCA30.2952755905511811No Hit
AACCTAGGGAAGCAGTCGTATCAACGCAGAGTCGACTTTT20.19685039370078738No Hit
AACCTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCT20.19685039370078738No Hit
AACCTAGGGATCCTGGGCCATTGGCCTCTCTGTGGCAGAC20.19685039370078738No Hit
AACCTAGGGGACTTACAGCGTATGTAGCACAAGTGTAACC20.19685039370078738No Hit
AACCTAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT20.19685039370078738No Hit
AACCTAGGGAAGAAGTGGTATCAACCCAAAAAAAAAAAAA20.19685039370078738No Hit
AACCTAGGGGAAATTGAAATTTGTGATCTTTTTAGAATAA20.19685039370078738No Hit
AACCTAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAG20.19685039370078738No Hit
AACCTAGGGAGCATACAAATTTTAAAGTAACCAGAATCGT20.19685039370078738No Hit
AACCTAGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.19685039370078738No Hit
AACCTAGGGTCAACGATCCTCTTCTGAGATGAGTTTTTGT20.19685039370078738No Hit
AACCTAGGGGCCCGTGCAGAGCAGACAGAGGGCCGCATAA20.19685039370078738No Hit
AACCTAGGGATGTCTTGTGCACTAAGCTGGGCTAGTTCAG20.19685039370078738No Hit
AACCTAGGGGTTCTTCAGGCTCATTTGCTGTGCTGGTTTT20.19685039370078738No Hit
AACCTAGGGAGACTTAGGACCCTACTGTCCTTTGGGCTCT20.19685039370078738No Hit
AACCTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGAT20.19685039370078738No Hit
AACCTAGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT20.19685039370078738No Hit
AACCTAGGGTCACAGATCCTCTTCTGAGATGCGTTTTTGT20.19685039370078738No Hit
AACCTAGGGTCAAAGATCCTCTCCTGAAGGAGATTGGGCG20.19685039370078738No Hit
AACCTAGGGTCACAGATCCTCTTCTGAAGGACATTGGGCG20.19685039370078738No Hit
AACCTAGGGGCCCTTTCTTGGTGTGTGGGGGATCTATGGC20.19685039370078738No Hit
AACCTAGGGGATGAAAAATGAGCAAAAGCACACTTCGCCA20.19685039370078738No Hit
AACCTAGGGAACTCATCAAATGTAGCACTTTTTGTTTCTG20.19685039370078738No Hit
AACCTAGGGTCACCTTCATCTTTTTCACTGGAGCCTTCCT20.19685039370078738No Hit
AACCTAGGGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19685039370078738No Hit
AACCTAGGGCATTGTATCTCTGTCTGATGCAAAAAGGTAG20.19685039370078738No Hit
AACCTAGGGATAGCAGGATTTGTTCACTGATTCCCATTAT20.19685039370078738No Hit
AACCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCG20.19685039370078738No Hit
AACCTAGGGTCAAAGATCCTATTCTGAGATGAGTTTTTGT20.19685039370078738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCTAG1000.033.751
CTAGGGG352.4706424E-733.754
CTAGGGA550.033.754
TAGGGGG200.001723563833.755
ACCTAGG1000.033.752
TAGGGAA200.001723563833.755
CCTAGGG1000.033.753
Produced by FastQC (version 0.11.5)