##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525887_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1016 Sequences flagged as poor quality 0 Sequence length 40 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.32283464566929 4.0 4.0 27.0 4.0 31.0 2 10.146653543307087 4.0 4.0 16.0 4.0 27.0 3 8.020669291338583 4.0 4.0 10.0 4.0 19.0 4 9.062007874015748 4.0 4.0 14.0 4.0 23.0 5 9.201771653543307 4.0 4.0 13.0 4.0 25.0 6 8.941929133858268 4.0 4.0 11.0 4.0 23.0 7 9.983267716535433 4.0 4.0 18.0 4.0 26.0 8 10.354330708661417 4.0 4.0 18.0 4.0 29.0 9 10.021653543307087 4.0 4.0 16.0 4.0 29.0 10 9.156496062992126 4.0 4.0 11.0 4.0 26.0 11 10.184055118110237 4.0 4.0 14.0 4.0 31.0 12 9.65748031496063 4.0 4.0 14.0 4.0 29.0 13 9.0501968503937 4.0 4.0 10.0 4.0 26.0 14 9.565944881889763 4.0 4.0 10.0 4.0 30.0 15 9.499015748031496 4.0 4.0 4.0 4.0 30.0 16 8.55216535433071 4.0 4.0 4.0 4.0 26.0 17 8.15748031496063 4.0 4.0 4.0 4.0 25.0 18 7.824803149606299 4.0 4.0 4.0 4.0 23.0 19 7.724409448818897 4.0 4.0 4.0 4.0 24.0 20 8.043307086614174 4.0 4.0 4.0 4.0 26.0 21 7.318897637795276 4.0 4.0 4.0 4.0 22.0 22 7.205708661417323 4.0 4.0 4.0 4.0 20.0 23 7.368110236220472 4.0 4.0 4.0 4.0 21.0 24 7.150590551181103 4.0 4.0 4.0 4.0 20.0 25 7.4202755905511815 4.0 4.0 4.0 4.0 22.0 26 7.358267716535433 4.0 4.0 4.0 4.0 22.0 27 7.573818897637795 4.0 4.0 4.0 4.0 24.0 28 7.738188976377953 4.0 4.0 4.0 4.0 25.0 29 7.21751968503937 4.0 4.0 4.0 4.0 22.0 30 7.197834645669292 4.0 4.0 4.0 4.0 22.0 31 7.622047244094488 4.0 4.0 4.0 4.0 24.0 32 7.655511811023622 4.0 4.0 4.0 4.0 26.0 33 7.3858267716535435 4.0 4.0 4.0 4.0 23.0 34 7.682086614173229 4.0 4.0 4.0 4.0 25.0 35 7.405511811023622 4.0 4.0 4.0 4.0 24.0 36 7.730314960629921 4.0 4.0 4.0 4.0 29.0 37 7.458661417322834 4.0 4.0 4.0 4.0 25.0 38 7.422244094488189 4.0 4.0 4.0 4.0 24.0 39 7.15255905511811 4.0 4.0 4.0 4.0 23.0 40 7.381889763779528 4.0 4.0 4.0 4.0 28.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 5 1 1.2602564102564102 5 2 1.920192307692309 5 3 0.5756410256410263 5 4 3.2355769230769234 5 5 3.852564102564102 5 6 -0.10576923076923173 5 7 4.158653846153847 5 8 3.3878205128205128 5 9 3.490384615384615 5 10 5.333333333333332 5 11 1.4663461538461533 5 12 -4.116987179487179 5 13 -2.065705128205127 5 14 2.5016025641025657 5 15 -6.886217948717949 5 16 -6.116987179487179 5 17 -4.915064102564102 5 18 -4.766025641025639 5 19 -5.033653846153847 5 20 -4.820512820512819 5 21 -3.8942307692307683 5 22 -3.2740384615384617 5 23 -3.6298076923076916 5 24 -3.0753205128205128 5 25 -3.144230769230769 5 26 -3.5801282051282053 5 27 -3.5544871794871806 5 28 -3.6506410256410255 5 29 -3.0785256410256405 5 30 -3.2355769230769234 5 31 -4.195512820512819 5 32 -4.608974358974358 5 33 -3.5464743589743595 5 34 -4.514423076923077 5 35 -4.613782051282051 5 36 -4.403846153846153 5 37 -4.557692307692308 5 38 -4.038461538461538 5 39 -3.717948717948717 5 40 -4.302884615384615 10 1 7.26025641025641 10 2 7.253525641025643 10 3 5.575641025641026 10 4 4.902243589743589 10 5 4.852564102564102 10 6 10.560897435897434 10 7 11.49198717948718 10 8 10.721153846153847 10 9 12.823717948717949 10 10 1.666666666666666 10 11 18.79967948717949 10 12 23.88301282051282 10 13 25.267628205128204 10 14 24.834935897435898 10 15 25.11378205128205 10 16 22.88301282051282 10 17 18.084935897435898 10 18 24.23397435897436 10 19 17.966346153846153 10 20 21.179487179487182 10 21 28.105769230769234 10 22 23.72596153846154 10 23 29.370192307692307 10 24 24.92467948717949 10 25 23.85576923076923 10 26 17.419871794871796 10 27 13.44551282051282 10 28 19.349358974358974 10 29 1.9214743589743595 10 30 7.764423076923077 10 31 18.804487179487182 10 32 18.391025641025642 10 33 10.45352564102564 10 34 18.485576923076923 10 35 17.38621794871795 10 36 29.596153846153847 10 37 22.442307692307693 10 38 29.96153846153846 10 39 30.282051282051285 10 40 29.697115384615387 16 1 -7.73974358974359 16 2 -4.746474358974357 16 3 -4.424358974358974 16 4 -4.097756410256411 16 5 -4.147435897435898 16 6 -4.439102564102566 16 7 -5.508012820512819 16 8 -6.278846153846153 16 9 -6.176282051282051 16 10 -4.333333333333334 16 11 -6.200320512820513 16 12 -6.116987179487179 16 13 -5.732371794871794 16 14 -6.1650641025641 16 15 -6.886217948717949 16 16 -6.116987179487179 16 17 -4.915064102564102 16 18 -4.766025641025639 16 19 -5.033653846153847 16 20 -4.820512820512819 16 21 -3.8942307692307683 16 22 -3.2740384615384617 16 23 -3.6298076923076916 16 24 -3.0753205128205128 16 25 -3.144230769230769 16 26 -3.5801282051282053 16 27 -3.5544871794871806 16 28 -3.6506410256410255 16 29 -3.0785256410256405 16 30 -3.2355769230769234 16 31 -4.195512820512819 16 32 -4.608974358974358 16 33 -3.5464743589743595 16 34 -4.514423076923077 16 35 -4.613782051282051 16 36 -4.403846153846153 16 37 -4.557692307692308 16 38 -4.038461538461538 16 39 -3.717948717948717 16 40 -4.302884615384615 18 1 12.76025641025641 18 2 11.503525641025643 18 3 8.075641025641026 18 4 7.402243589743589 18 5 13.352564102564102 18 6 18.060897435897434 18 7 15.99198717948718 18 8 15.471153846153847 18 9 16.57371794871795 18 10 18.916666666666664 18 11 17.79967948717949 18 12 17.88301282051282 18 13 14.267628205128206 18 14 18.834935897435898 18 15 17.36378205128205 18 16 7.633012820512821 18 17 14.084935897435898 18 18 3.7339743589743613 18 19 10.966346153846153 18 20 1.1794871794871806 18 21 2.1057692307692317 18 22 1.2259615384615383 18 23 8.620192307692308 18 24 6.424679487179487 18 25 7.355769230769231 18 26 8.419871794871796 18 27 6.945512820512819 18 28 7.5993589743589745 18 29 7.6714743589743595 18 30 8.014423076923077 18 31 10.30448717948718 18 32 9.641025641025642 18 33 8.95352564102564 18 34 10.985576923076923 18 35 8.636217948717949 18 36 6.346153846153847 18 37 8.942307692307692 18 38 8.211538461538462 18 39 7.532051282051283 18 40 9.697115384615385 19 1 19.26025641025641 19 2 2.253525641025643 19 3 22.575641025641026 19 4 20.90224358974359 19 5 20.852564102564102 19 6 11.560897435897434 19 7 18.491987179487182 19 8 21.721153846153847 19 9 22.82371794871795 19 10 4.666666666666666 19 11 13.799679487179487 19 12 21.88301282051282 19 13 23.267628205128204 19 14 17.834935897435898 19 15 26.11378205128205 19 16 20.88301282051282 19 17 4.084935897435898 19 18 19.23397435897436 19 19 20.966346153846153 19 20 3.1794871794871806 19 21 18.105769230769234 19 22 13.725961538461538 19 23 3.3701923076923084 19 24 1.9246794871794872 19 25 13.85576923076923 19 26 23.419871794871796 19 27 12.44551282051282 19 28 7.3493589743589745 19 29 20.921474358974358 19 30 16.764423076923077 19 31 24.804487179487182 19 32 27.391025641025642 19 33 22.453525641025642 19 34 24.485576923076923 19 35 27.38621794871795 19 36 -4.403846153846153 19 37 -4.557692307692308 19 38 -4.038461538461538 19 39 -3.717948717948717 19 40 -4.302884615384615 25 1 10.26025641025641 25 2 3.253525641025643 25 3 7.575641025641026 25 4 13.90224358974359 25 5 20.852564102564102 25 6 17.560897435897434 25 7 13.49198717948718 25 8 19.721153846153847 25 9 18.82371794871795 25 10 16.666666666666664 25 11 27.79967948717949 25 12 25.88301282051282 25 13 28.267628205128204 25 14 27.834935897435898 25 15 20.11378205128205 25 16 26.88301282051282 25 17 27.084935897435898 25 18 26.23397435897436 25 19 26.966346153846153 25 20 28.179487179487182 25 21 21.105769230769234 25 22 4.725961538461538 25 23 17.370192307692307 25 24 2.9246794871794872 25 25 2.855769230769231 25 26 15.419871794871796 25 27 8.44551282051282 25 28 19.349358974358974 25 29 9.92147435897436 25 30 3.7644230769230766 25 31 17.804487179487182 25 32 22.391025641025642 25 33 16.453525641025642 25 34 22.485576923076923 25 35 22.38621794871795 25 36 20.596153846153847 25 37 25.442307692307693 25 38 20.96153846153846 25 39 9.282051282051283 25 40 24.697115384615387 28 1 18.01025641025641 28 2 0.0035256410256430115 28 3 4.825641025641026 28 4 10.40224358974359 28 5 11.852564102564102 28 6 -0.18910256410256565 28 7 8.74198717948718 28 8 13.471153846153847 28 9 6.823717948717949 28 10 9.166666666666666 28 11 1.0496794871794872 28 12 0.8830128205128212 28 13 2.017628205128206 28 14 0.8349358974358996 28 15 -1.386217948717949 28 16 2.633012820512821 28 17 3.084935897435898 28 18 0.9839743589743613 28 19 2.2163461538461533 28 20 -0.5705128205128194 28 21 -0.8942307692307683 28 22 -1.5240384615384617 28 23 -0.6298076923076916 28 24 -1.3253205128205128 28 25 3.605769230769231 28 26 1.6698717948717947 28 27 3.6955128205128194 28 28 3.8493589743589745 28 29 1.4214743589743595 28 30 4.264423076923077 28 31 2.3044871794871806 28 32 1.1410256410256423 28 33 4.2035256410256405 28 34 2.7355769230769234 28 35 2.636217948717949 28 36 3.3461538461538467 28 37 3.1923076923076916 28 38 3.7115384615384617 28 39 4.032051282051283 28 40 3.447115384615385 31 1 10.26025641025641 31 2 13.253525641025643 31 3 13.575641025641026 31 4 4.902243589743589 31 5 1.8525641025641022 31 6 1.5608974358974343 31 7 17.491987179487182 31 8 13.721153846153847 31 9 16.82371794871795 31 10 0.6666666666666661 31 11 22.79967948717949 31 12 19.88301282051282 31 13 4.267628205128206 31 14 22.834935897435898 31 15 22.11378205128205 31 16 19.88301282051282 31 17 -4.915064102564102 31 18 -4.766025641025639 31 19 -5.033653846153847 31 20 -4.820512820512819 31 21 -3.8942307692307683 31 22 -3.2740384615384617 31 23 -3.6298076923076916 31 24 -3.0753205128205128 31 25 -3.144230769230769 31 26 -3.5801282051282053 31 27 -3.5544871794871806 31 28 -3.6506410256410255 31 29 -3.0785256410256405 31 30 -3.2355769230769234 31 31 -4.195512820512819 31 32 -4.608974358974358 31 33 -3.5464743589743595 31 34 -4.514423076923077 31 35 -4.613782051282051 31 36 -4.403846153846153 31 37 -4.557692307692308 31 38 -4.038461538461538 31 39 -3.717948717948717 31 40 -4.302884615384615 37 1 -7.73974358974359 37 2 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-4.746474358974357 39 3 -4.424358974358974 39 4 -4.097756410256411 39 5 -4.147435897435898 39 6 -4.439102564102566 39 7 -5.508012820512819 39 8 -6.278846153846153 39 9 -6.176282051282051 39 10 -4.333333333333334 39 11 -6.200320512820513 39 12 -6.116987179487179 39 13 -5.732371794871794 39 14 -6.1650641025641 39 15 -6.886217948717949 39 16 -6.116987179487179 39 17 -4.915064102564102 39 18 -4.766025641025639 39 19 -5.033653846153847 39 20 -4.820512820512819 39 21 -3.8942307692307683 39 22 -3.2740384615384617 39 23 -3.6298076923076916 39 24 -3.0753205128205128 39 25 -3.144230769230769 39 26 -3.5801282051282053 39 27 -3.5544871794871806 39 28 -3.6506410256410255 39 29 -3.0785256410256405 39 30 -3.2355769230769234 39 31 -4.195512820512819 39 32 -4.608974358974358 39 33 -3.5464743589743595 39 34 -4.514423076923077 39 35 -4.613782051282051 39 36 -4.403846153846153 39 37 -4.557692307692308 39 38 -4.038461538461538 39 39 -3.717948717948717 39 40 -4.302884615384615 40 1 11.26025641025641 40 2 10.253525641025643 40 3 18.575641025641026 40 4 7.902243589743589 40 5 5.852564102564102 40 6 3.5608974358974343 40 7 3.4919871794871806 40 8 13.721153846153847 40 9 14.823717948717949 40 10 11.666666666666666 40 11 -0.20032051282051277 40 12 10.883012820512821 40 13 9.267628205128206 40 14 0.8349358974358996 40 15 18.11378205128205 40 16 17.88301282051282 40 17 19.084935897435898 40 18 17.23397435897436 40 19 8.966346153846153 40 20 11.17948717948718 40 21 16.105769230769234 40 22 8.725961538461538 40 23 11.370192307692308 40 24 7.924679487179487 40 25 8.85576923076923 40 26 19.419871794871796 40 27 10.44551282051282 40 28 16.349358974358974 40 29 18.921474358974358 40 30 12.764423076923077 40 31 22.804487179487182 40 32 22.391025641025642 40 33 15.45352564102564 40 34 16.485576923076923 40 35 24.38621794871795 40 36 19.596153846153847 40 37 27.442307692307693 40 38 19.96153846153846 40 39 28.282051282051285 40 40 24.697115384615387 45 1 12.76025641025641 45 2 16.753525641025643 45 3 3.0756410256410263 45 4 8.90224358974359 45 5 9.852564102564102 45 6 11.560897435897434 45 7 7.991987179487181 45 8 10.721153846153847 45 9 6.823717948717949 45 10 -0.8333333333333339 45 11 10.299679487179487 45 12 3.883012820512821 45 13 7.767628205128206 45 14 3.8349358974358996 45 15 4.113782051282051 45 16 17.88301282051282 45 17 6.584935897435898 45 18 17.23397435897436 45 19 16.466346153846153 45 20 15.67948717948718 45 21 21.105769230769234 45 22 20.22596153846154 45 23 24.370192307692307 45 24 22.42467948717949 45 25 23.35576923076923 45 26 15.419871794871796 45 27 21.945512820512818 45 28 20.849358974358974 45 29 14.92147435897436 45 30 16.764423076923077 45 31 16.804487179487182 45 32 20.391025641025642 45 33 14.45352564102564 45 34 16.485576923076923 45 35 16.38621794871795 45 36 24.596153846153847 45 37 25.942307692307693 45 38 12.961538461538462 45 39 13.282051282051283 45 40 11.697115384615385 49 1 21.26025641025641 49 2 2.253525641025643 49 3 24.575641025641026 49 4 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-4.195512820512819 109 32 -4.608974358974358 109 33 -3.5464743589743595 109 34 -4.514423076923077 109 35 -4.613782051282051 109 36 -4.403846153846153 109 37 -4.557692307692308 109 38 -4.038461538461538 109 39 -3.717948717948717 109 40 -4.302884615384615 110 1 -7.73974358974359 110 2 -4.746474358974357 110 3 -4.424358974358974 110 4 -4.097756410256411 110 5 -4.147435897435898 110 6 -4.439102564102566 110 7 -5.508012820512819 110 8 -6.278846153846153 110 9 -6.176282051282051 110 10 -4.333333333333334 110 11 -6.200320512820513 110 12 -6.116987179487179 110 13 -5.732371794871794 110 14 -6.1650641025641 110 15 -6.886217948717949 110 16 -6.116987179487179 110 17 -4.915064102564102 110 18 -4.766025641025639 110 19 -5.033653846153847 110 20 -4.820512820512819 110 21 -3.8942307692307683 110 22 -3.2740384615384617 110 23 -3.6298076923076916 110 24 -3.0753205128205128 110 25 -3.144230769230769 110 26 -3.5801282051282053 110 27 -3.5544871794871806 110 28 -3.6506410256410255 110 29 -3.0785256410256405 110 30 -3.2355769230769234 110 31 -4.195512820512819 110 32 -4.608974358974358 110 33 -3.5464743589743595 110 34 -4.514423076923077 110 35 -4.613782051282051 110 36 -4.403846153846153 110 37 -4.557692307692308 110 38 -4.038461538461538 110 39 -3.717948717948717 110 40 -4.302884615384615 111 1 14.26025641025641 111 2 15.253525641025643 111 3 9.575641025641026 111 4 7.902243589743589 111 5 4.852564102564102 111 6 21.560897435897434 111 7 20.491987179487182 111 8 17.721153846153847 111 9 15.823717948717949 111 10 13.666666666666666 111 11 0.7996794871794872 111 12 16.88301282051282 111 13 13.267628205128206 111 14 9.8349358974359 111 15 17.11378205128205 111 16 8.883012820512821 111 17 14.084935897435898 111 18 18.23397435897436 111 19 23.966346153846153 111 20 24.179487179487182 111 21 -3.8942307692307683 111 22 -3.2740384615384617 111 23 -3.6298076923076916 111 24 -3.0753205128205128 111 25 -3.144230769230769 111 26 -3.5801282051282053 111 27 -3.5544871794871806 111 28 -3.6506410256410255 111 29 -3.0785256410256405 111 30 -3.2355769230769234 111 31 -4.195512820512819 111 32 -4.608974358974358 111 33 -3.5464743589743595 111 34 -4.514423076923077 111 35 -4.613782051282051 111 36 -4.403846153846153 111 37 -4.557692307692308 111 38 -4.038461538461538 111 39 -3.717948717948717 111 40 -4.302884615384615 114 1 -7.73974358974359 114 2 -4.746474358974357 114 3 -4.424358974358974 114 4 -4.097756410256411 114 5 -4.147435897435898 114 6 -4.439102564102566 114 7 -5.508012820512819 114 8 -6.278846153846153 114 9 -6.176282051282051 114 10 -4.333333333333334 114 11 -6.200320512820513 114 12 -6.116987179487179 114 13 -5.732371794871794 114 14 -6.1650641025641 114 15 -6.886217948717949 114 16 -6.116987179487179 114 17 -4.915064102564102 114 18 -4.766025641025639 114 19 -5.033653846153847 114 20 -4.820512820512819 114 21 -3.8942307692307683 114 22 -3.2740384615384617 114 23 -3.6298076923076916 114 24 -3.0753205128205128 114 25 -3.144230769230769 114 26 -3.5801282051282053 114 27 -3.5544871794871806 114 28 -3.6506410256410255 114 29 -3.0785256410256405 114 30 -3.2355769230769234 114 31 -4.195512820512819 114 32 -4.608974358974358 114 33 -3.5464743589743595 114 34 -4.514423076923077 114 35 -4.613782051282051 114 36 -4.403846153846153 114 37 -4.557692307692308 114 38 -4.038461538461538 114 39 -3.717948717948717 114 40 -4.302884615384615 116 1 -7.73974358974359 116 2 -4.746474358974357 116 3 -4.424358974358974 116 4 -4.097756410256411 116 5 -4.147435897435898 116 6 -4.439102564102566 116 7 -5.508012820512819 116 8 -6.278846153846153 116 9 -6.176282051282051 116 10 -4.333333333333334 116 11 -6.200320512820513 116 12 -6.116987179487179 116 13 -5.732371794871794 116 14 -6.1650641025641 116 15 -6.886217948717949 116 16 -6.116987179487179 116 17 -4.915064102564102 116 18 -4.766025641025639 116 19 -5.033653846153847 116 20 -4.820512820512819 116 21 -3.8942307692307683 116 22 -3.2740384615384617 116 23 -3.6298076923076916 116 24 -3.0753205128205128 116 25 -3.144230769230769 116 26 -3.5801282051282053 116 27 -3.5544871794871806 116 28 -3.6506410256410255 116 29 -3.0785256410256405 116 30 -3.2355769230769234 116 31 -4.195512820512819 116 32 -4.608974358974358 116 33 -3.5464743589743595 116 34 -4.514423076923077 116 35 -4.613782051282051 116 36 -4.403846153846153 116 37 -4.557692307692308 116 38 -4.038461538461538 116 39 -3.717948717948717 116 40 -4.302884615384615 117 1 -7.73974358974359 117 2 -4.746474358974357 117 3 -4.424358974358974 117 4 -4.097756410256411 117 5 -4.147435897435898 117 6 -4.439102564102566 117 7 -5.508012820512819 117 8 -6.278846153846153 117 9 -6.176282051282051 117 10 -4.333333333333334 117 11 -6.200320512820513 117 12 -6.116987179487179 117 13 -5.732371794871794 117 14 -6.1650641025641 117 15 -6.886217948717949 117 16 -6.116987179487179 117 17 -4.915064102564102 117 18 -4.766025641025639 117 19 -5.033653846153847 117 20 -4.820512820512819 117 21 -3.8942307692307683 117 22 -3.2740384615384617 117 23 -3.6298076923076916 117 24 -3.0753205128205128 117 25 -3.144230769230769 117 26 -3.5801282051282053 117 27 -3.5544871794871806 117 28 -3.6506410256410255 117 29 -3.0785256410256405 117 30 -3.2355769230769234 117 31 -4.195512820512819 117 32 -4.608974358974358 117 33 -3.5464743589743595 117 34 -4.514423076923077 117 35 -4.613782051282051 117 36 -4.403846153846153 117 37 -4.557692307692308 117 38 -4.038461538461538 117 39 -3.717948717948717 117 40 -4.302884615384615 118 1 -7.73974358974359 118 2 -4.746474358974357 118 3 -4.424358974358974 118 4 -4.097756410256411 118 5 -4.147435897435898 118 6 -4.439102564102566 118 7 -5.508012820512819 118 8 -6.278846153846153 118 9 -6.176282051282051 118 10 -4.333333333333334 118 11 -6.200320512820513 118 12 -6.116987179487179 118 13 -5.732371794871794 118 14 -6.1650641025641 118 15 -6.886217948717949 118 16 -6.116987179487179 118 17 -4.915064102564102 118 18 -4.766025641025639 118 19 -5.033653846153847 118 20 -4.820512820512819 118 21 -3.8942307692307683 118 22 -3.2740384615384617 118 23 -3.6298076923076916 118 24 -3.0753205128205128 118 25 -3.144230769230769 118 26 -3.5801282051282053 118 27 -3.5544871794871806 118 28 -3.6506410256410255 118 29 -3.0785256410256405 118 30 -3.2355769230769234 118 31 -4.195512820512819 118 32 -4.608974358974358 118 33 -3.5464743589743595 118 34 -4.514423076923077 118 35 -4.613782051282051 118 36 -4.403846153846153 118 37 -4.557692307692308 118 38 -4.038461538461538 118 39 -3.717948717948717 118 40 -4.302884615384615 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 667.0 5 24.0 6 18.0 7 8.0 8 12.0 9 15.0 10 19.0 11 20.0 12 13.0 13 18.0 14 11.0 15 15.0 16 6.0 17 4.0 18 3.0 19 11.0 20 30.0 21 18.0 22 31.0 23 16.0 24 16.0 25 9.0 26 13.0 27 7.0 28 4.0 29 4.0 30 3.0 31 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 96.64694280078896 1.6765285996055226 0.4930966469428008 1.183431952662722 8 95.26627218934911 2.465483234714004 1.4792899408284024 0.7889546351084813 9 96.45669291338582 1.7716535433070866 0.984251968503937 0.7874015748031495 10 45.463510848126234 26.035502958579883 19.62524654832347 8.875739644970414 11 27.85433070866142 25.29527559055118 19.19291338582677 27.65748031496063 12 27.559055118110237 32.57874015748031 20.57086614173228 19.291338582677163 13 19.92110453648915 26.13412228796844 24.45759368836292 29.48717948717949 14 17.913385826771652 36.90944881889764 24.507874015748033 20.669291338582678 15 38.38582677165354 22.539370078740156 23.917322834645667 15.157480314960631 16 25.88582677165354 35.039370078740156 25.29527559055118 13.779527559055119 17 23.523622047244093 25.098425196850393 36.22047244094488 15.157480314960631 18 25.492125984251967 23.62204724409449 24.015748031496063 26.87007874015748 19 20.669291338582678 24.70472440944882 26.37795275590551 28.248031496062993 20 20.47244094488189 25.787401574803148 36.318897637795274 17.421259842519685 21 21.358267716535433 22.933070866141733 27.95275590551181 27.755905511811026 22 20.669291338582678 22.34251968503937 37.696850393700785 19.291338582677163 23 20.295566502463057 23.054187192118228 35.172413793103445 21.47783251231527 24 17.716535433070867 29.7244094488189 25.88582677165354 26.673228346456696 25 21.893491124260358 24.65483234714004 33.33333333333333 20.118343195266274 26 32.77393879565646 25.962487660414613 22.90227048371175 18.36130306021718 27 20.138203356367228 36.92003948667325 21.816386969397826 21.1253701875617 28 24.2603550295858 33.33333333333333 22.781065088757398 19.62524654832347 29 31.454005934718097 32.34421364985163 19.683481701285853 16.51829871414441 30 26.502463054187192 26.108374384236456 28.866995073891626 18.52216748768473 31 26.87007874015748 30.01968503937008 23.228346456692915 19.88188976377953 32 29.191321499013807 30.96646942800789 23.076923076923077 16.765285996055226 33 30.511811023622048 28.1496062992126 23.12992125984252 18.208661417322837 34 20.57086614173228 23.72047244094488 38.77952755905512 16.92913385826772 35 21.062992125984252 26.08267716535433 36.71259842519685 16.141732283464567 36 23.24431256181998 27.398615232443124 30.66271018793274 18.694362017804153 37 26.08267716535433 27.460629921259844 31.10236220472441 15.354330708661418 38 27.389162561576352 25.02463054187192 28.57142857142857 19.014778325123153 39 24.606299212598426 27.36220472440945 21.8503937007874 26.181102362204722 40 22.539370078740156 28.444881889763778 31.39763779527559 17.61811023622047 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 1.0 11 1.5 12 2.0 13 2.0 14 4.5 15 7.0 16 7.5 17 8.0 18 8.0 19 7.5 20 7.0 21 8.5 22 10.0 23 10.0 24 9.0 25 8.0 26 8.5 27 9.0 28 9.0 29 15.5 30 22.0 31 24.0 32 26.0 33 26.0 34 30.5 35 35.0 36 40.0 37 45.0 38 45.0 39 54.0 40 63.0 41 80.5 42 98.0 43 98.0 44 88.5 45 79.0 46 83.5 47 88.0 48 88.0 49 99.0 50 110.0 51 124.0 52 138.0 53 138.0 54 108.0 55 78.0 56 72.5 57 67.0 58 67.0 59 58.5 60 50.0 61 40.5 62 31.0 63 31.0 64 24.0 65 17.0 66 13.0 67 9.0 68 9.0 69 7.0 70 5.0 71 3.5 72 2.0 73 2.0 74 1.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.19685039370078738 8 0.19685039370078738 9 0.0 10 0.19685039370078738 11 0.0 12 0.0 13 0.19685039370078738 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.09842519685039369 24 0.0 25 0.19685039370078738 26 0.2952755905511811 27 0.2952755905511811 28 0.19685039370078738 29 0.4921259842519685 30 0.09842519685039369 31 0.0 32 0.19685039370078738 33 0.0 34 0.0 35 0.0 36 0.4921259842519685 37 0.0 38 0.09842519685039369 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1016.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.43700787401575 #Duplication Level Percentage of deduplicated Percentage of total 1 96.23250807319698 87.99212598425197 2 3.1216361679224973 5.708661417322835 3 0.2152852529601722 0.5905511811023622 4 0.1076426264800861 0.39370078740157477 5 0.0 0.0 6 0.0 0.0 7 0.1076426264800861 0.6889763779527559 8 0.0 0.0 9 0.0 0.0 >10 0.2152852529601722 4.625984251968504 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 24 2.3622047244094486 No Hit AACCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 23 2.263779527559055 No Hit AACCTAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 7 0.6889763779527559 No Hit AACCTAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 4 0.39370078740157477 No Hit AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 3 0.2952755905511811 No Hit AACCTAGGGAGACACTGATGGACTTTGTGACTATGGGCCA 3 0.2952755905511811 No Hit AACCTAGGGAAGCAGTCGTATCAACGCAGAGTCGACTTTT 2 0.19685039370078738 No Hit AACCTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCT 2 0.19685039370078738 No Hit AACCTAGGGATCCTGGGCCATTGGCCTCTCTGTGGCAGAC 2 0.19685039370078738 No Hit AACCTAGGGGACTTACAGCGTATGTAGCACAAGTGTAACC 2 0.19685039370078738 No Hit AACCTAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT 2 0.19685039370078738 No Hit AACCTAGGGAAGAAGTGGTATCAACCCAAAAAAAAAAAAA 2 0.19685039370078738 No Hit AACCTAGGGGAAATTGAAATTTGTGATCTTTTTAGAATAA 2 0.19685039370078738 No Hit AACCTAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAG 2 0.19685039370078738 No Hit AACCTAGGGAGCATACAAATTTTAAAGTAACCAGAATCGT 2 0.19685039370078738 No Hit AACCTAGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.19685039370078738 No Hit AACCTAGGGTCAACGATCCTCTTCTGAGATGAGTTTTTGT 2 0.19685039370078738 No Hit AACCTAGGGGCCCGTGCAGAGCAGACAGAGGGCCGCATAA 2 0.19685039370078738 No Hit AACCTAGGGATGTCTTGTGCACTAAGCTGGGCTAGTTCAG 2 0.19685039370078738 No Hit AACCTAGGGGTTCTTCAGGCTCATTTGCTGTGCTGGTTTT 2 0.19685039370078738 No Hit AACCTAGGGAGACTTAGGACCCTACTGTCCTTTGGGCTCT 2 0.19685039370078738 No Hit AACCTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGAT 2 0.19685039370078738 No Hit AACCTAGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT 2 0.19685039370078738 No Hit AACCTAGGGTCACAGATCCTCTTCTGAGATGCGTTTTTGT 2 0.19685039370078738 No Hit AACCTAGGGTCAAAGATCCTCTCCTGAAGGAGATTGGGCG 2 0.19685039370078738 No Hit AACCTAGGGTCACAGATCCTCTTCTGAAGGACATTGGGCG 2 0.19685039370078738 No Hit AACCTAGGGGCCCTTTCTTGGTGTGTGGGGGATCTATGGC 2 0.19685039370078738 No Hit AACCTAGGGGATGAAAAATGAGCAAAAGCACACTTCGCCA 2 0.19685039370078738 No Hit AACCTAGGGAACTCATCAAATGTAGCACTTTTTGTTTCTG 2 0.19685039370078738 No Hit AACCTAGGGTCACCTTCATCTTTTTCACTGGAGCCTTCCT 2 0.19685039370078738 No Hit AACCTAGGGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.19685039370078738 No Hit AACCTAGGGCATTGTATCTCTGTCTGATGCAAAAAGGTAG 2 0.19685039370078738 No Hit AACCTAGGGATAGCAGGATTTGTTCACTGATTCCCATTAT 2 0.19685039370078738 No Hit AACCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCG 2 0.19685039370078738 No Hit AACCTAGGGTCAAAGATCCTATTCTGAGATGAGTTTTTGT 2 0.19685039370078738 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCTAG 100 0.0 33.75 1 CTAGGGG 35 2.4706424E-7 33.75 4 CTAGGGA 55 0.0 33.75 4 TAGGGGG 20 0.0017235638 33.75 5 ACCTAGG 100 0.0 33.75 2 TAGGGAA 20 0.0017235638 33.75 5 CCTAGGG 100 0.0 33.75 3 >>END_MODULE