SRR1525886_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1525886_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26334
Sequences flagged as poor quality	0
Sequence length	56
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	346	1.313890787574998	No Hit
TGTCACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	192	0.7290954659375711	No Hit
TGTCACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	170	0.6455532771322244	No Hit
TGTCACGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	149	0.5658084605453026	No Hit
TGTCACGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	114	0.4329004329004329	No Hit
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	111	0.42150831624515833	No Hit
TGTCACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	80	0.303789777473988	No Hit
TGTCACGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	73	0.27720817194501407	No Hit
TGTCACGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC	63	0.23923444976076555	No Hit
TGTCACGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	63	0.23923444976076555	No Hit
TGTCACGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	59	0.22404496088706616	No Hit
TGTCACGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT	58	0.22024758866864128	No Hit
TGTCACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	55	0.20885547201336674	No Hit
TGTCACGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	51	0.19366598313966735	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	50	0.1898686109212425	No Hit
TGTCACGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG	49	0.18607123870281764	No Hit
TGTCACGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT	47	0.17847649426596796	No Hit
TGTCACGGGAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGA	47	0.17847649426596796	No Hit
TGTCACGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	46	0.1746791220475431	No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	42	0.1594896331738437	No Hit
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	41	0.15569226095541885	No Hit
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	41	0.15569226095541885	No Hit
TGTCACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	38	0.1443001443001443	No Hit
TGTCACGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTCATGGTTCCTTTGGTC	37	0.14050277208171943	No Hit
TGTCACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	36	0.1367053998632946	No Hit
TGTCACGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG	35	0.13290802764486975	No Hit
TGTCACGGGACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGG	34	0.1291106554264449	No Hit
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	33	0.12531328320802004	No Hit
TGTCACGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	33	0.12531328320802004	No Hit
TGTCACGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	33	0.12531328320802004	No Hit
TGTCACGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC	32	0.12151591098959522	No Hit
TGTCACGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG	31	0.11771853877117035	No Hit
TGTCACGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG	30	0.11392116655274549	No Hit
TGTCACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC	30	0.11392116655274549	No Hit
TGTCACGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA	30	0.11392116655274549	No Hit
TGTCACGGGGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACAT	28	0.10632642211589581	No Hit
TGTCACGGGCATTACCTTTATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAG	28	0.10632642211589581	No Hit
TGTCACGGGAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG	28	0.10632642211589581	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGGA	20	3.7552457E-4	50.916183	38
CTCGGAT	20	3.7552457E-4	50.916183	39
GCCCACG	15	0.0077604507	50.91618	38
TCGGATA	15	0.0077604507	50.91618	40
CCCACGG	15	0.0077604507	50.91618	39
CGGATAA	15	0.0077604507	50.91618	41
TACTCGG	20	3.8653033E-4	50.62016	37
TGCCCAC	15	0.007941937	50.620155	37
ATGCCCA	15	0.0080646705	50.42471	36
CATGCCC	15	0.0080646705	50.42471	35
TCCCAGG	15	0.008188809	50.23077	15
GACCCAC	20	4.0931057E-4	50.03832	9
GAAGCCA	15	0.008314364	50.038315	9
GATTGGA	15	0.008314364	50.038315	9
GACGCAC	15	0.008314364	50.038315	9
GATCCAA	15	0.008314364	50.038315	9
GCTCCTA	15	0.008314364	50.038315	9
TCAGCGG	20	4.2109375E-4	49.752384	27
CTCAGCG	20	4.2109375E-4	49.752384	26
CAGCGGT	20	4.2109375E-4	49.752384	28

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content