##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525886_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26334
Sequences flagged as poor quality	0
Sequence length	56
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.658768132452344	38.0	36.0	39.0	32.0	40.0
2	35.799346851978434	38.0	35.0	39.0	29.0	40.0
3	36.05582137161085	38.0	36.0	39.0	29.0	40.0
4	35.91824257613731	38.0	36.0	39.0	29.0	40.0
5	32.7398800030379	38.0	29.0	39.0	15.0	40.0
6	35.81320726057568	38.0	36.0	39.0	28.0	40.0
7	35.88368648894965	38.0	35.0	39.0	29.0	40.0
8	35.943760917445125	38.0	35.0	39.0	30.0	40.0
9	35.97387407913724	38.0	36.0	39.0	30.0	40.0
10	35.12762968026126	38.0	35.0	39.0	26.0	40.0
11	35.23877876509455	38.0	35.0	39.0	28.0	40.0
12	34.142591326801856	38.0	33.0	39.0	23.0	40.0
13	33.559011164274324	38.0	32.0	39.0	21.0	40.0
14	34.077010708589654	38.0	33.0	39.0	23.0	40.0
15	32.93776106934002	37.0	31.0	39.0	18.0	40.0
16	32.67380572643731	36.0	30.0	39.0	19.0	40.0
17	31.705741626794257	36.0	28.0	38.0	16.0	40.0
18	31.10742766005924	35.0	27.0	38.0	14.0	40.0
19	32.21280473912053	36.0	29.0	38.0	18.0	40.0
20	32.94300144300144	36.0	30.0	38.0	22.0	40.0
21	33.187780056201106	38.0	31.0	39.0	20.0	40.0
22	32.4925191767297	37.0	30.0	39.0	17.0	40.0
23	33.08285866180603	38.0	31.0	39.0	20.0	40.0
24	32.455570745044426	37.0	30.0	39.0	17.0	40.0
25	32.83891547049442	37.0	31.0	39.0	19.0	40.0
26	33.26817042606516	38.0	32.0	39.0	19.0	40.0
27	33.5988455988456	38.0	33.0	39.0	22.0	40.0
28	33.565808460545306	38.0	33.0	39.0	23.0	40.0
29	33.04040404040404	37.0	31.0	39.0	19.0	40.0
30	32.80458722563986	37.0	31.0	39.0	19.0	40.0
31	33.588516746411486	38.0	33.0	39.0	22.0	40.0
32	33.44277360066834	38.0	33.0	39.0	22.0	40.0
33	33.24337358547885	37.0	33.0	39.0	21.0	40.0
34	33.17027417027417	37.0	33.0	39.0	20.0	40.0
35	33.02901192374877	37.0	33.0	39.0	19.0	40.0
36	32.51773372826005	36.0	31.0	38.0	18.0	40.0
37	32.2374496848181	36.0	31.0	38.0	15.0	40.0
38	31.51868307131465	36.0	30.0	38.0	10.0	40.0
39	30.36207944102681	35.0	27.0	38.0	4.0	39.0
40	28.504442925495557	34.0	23.0	38.0	4.0	39.0
41	26.473114604693553	32.0	17.0	38.0	4.0	39.0
42	27.173501936659832	32.0	20.0	37.0	4.0	39.0
43	27.384294068504595	33.0	21.0	38.0	4.0	39.0
44	26.317726133515606	31.0	17.0	37.0	4.0	39.0
45	27.29137237031974	33.0	21.0	38.0	4.0	39.0
46	25.209652920179234	30.0	15.0	36.0	4.0	39.0
47	24.28719526087947	29.0	10.0	36.0	4.0	39.0
48	24.852320194425456	29.0	14.0	36.0	4.0	39.0
49	23.86458570669097	28.0	8.0	35.0	4.0	39.0
50	24.544467228677757	29.0	10.0	36.0	4.0	39.0
51	24.649198754461914	30.0	4.0	36.0	4.0	39.0
52	24.443646996278574	30.0	4.0	36.0	4.0	39.0
53	24.26384142173616	30.0	4.0	36.0	4.0	39.0
54	23.849244322928534	30.0	4.0	36.0	4.0	38.0
55	23.659603554340396	30.0	4.0	36.0	4.0	38.0
56	22.64365459102301	28.0	4.0	35.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	2.8221369836381527
1	2	3.233758070028344
1	3	3.6345594659388
1	4	3.477765457053458
1	5	7.6505855065804695
1	6	4.096624461230533
1	7	3.2309925261699064
1	8	3.0095130259895413
1	9	3.4083613406319913
1	10	3.072328974468199
1	11	-1.0337070378221753
1	12	-9.374898598672853
1	13	-18.319901732823283
1	14	-6.875190489934553
1	15	0.22160008659197672
1	16	-2.177633650500958
1	17	-6.661193834581855
1	18	-5.593957114389912
1	19	-0.802399935673435
1	20	-2.1444138264622907
1	21	5.362519698530569
1	22	6.446563259784405
1	23	5.643513781799346
1	24	6.375009797891
1	25	5.373964895623185
1	26	4.1162417557314654
1	27	5.187786362003212
1	28	4.558067835743117
1	29	5.7157650116907135
1	30	4.947673941030143
1	31	3.830985580726434
1	32	3.217206676030191
1	33	4.432955583810706
1	34	3.1465446316795145
1	35	4.535518689458623
1	36	3.766946009235088
1	37	4.246423795254358
1	38	4.915996458637313
1	39	3.818905609672985
1	40	-9.724200269301132
1	41	1.8759552023552928
1	42	0.18661507145568734
1	43	-9.360648361340463
1	44	-14.41975378986168
1	45	-1.3360362764537825
1	46	-4.843345300632013
1	47	-8.021143210298659
1	48	-4.263088628561793
1	49	-12.803120483703385
1	50	-2.7448971724352837
1	51	4.1037947517182225
1	52	4.382448683484476
1	53	3.496370044803438
1	54	3.8240284212695244
1	55	3.848068501069722
1	56	7.074253565443371
2	1	2.8996174741621203
2	2	3.9422307567619015
2	3	4.417168161590972
2	4	4.113774375671078
2	5	8.176226532221499
2	6	4.486256568253943
2	7	4.04035707466489
2	8	4.069713694885863
2	9	4.186510727477028
2	10	4.784703556407997
2	11	2.6279429064364663
2	12	-1.8091237937676112
2	13	0.5808786462179683
2	14	3.396827347300672
2	15	3.6179211568260854
2	16	4.00296835618801
2	17	5.968683534425949
2	18	2.6814052044506695
2	19	5.310755025307614
2	20	4.106422293939044
2	21	6.183032519043387
2	22	7.026830818312838
2	23	5.427237304653307
2	24	6.852155840254429
2	25	5.2909102690903005
2	26	4.259497051160672
2	27	3.9921341880901657
2	28	5.053051113335094
2	29	4.347872035102085
2	30	5.075879069235274
2	31	2.6252999619973423
2	32	2.40895695473699
2	33	2.3404249260626564
2	34	2.7390752894275643
2	35	3.944660272513243
2	36	4.459253701542782
2	37	4.735275523236524
2	38	4.764379959194724
2	39	5.966062800308439
2	40	3.3850527964736727
2	41	9.894907264785616
2	42	8.26855487078679
2	43	6.076363901758757
2	44	-1.9353613706866533
2	45	7.731410769254136
2	46	6.183410552210795
2	47	4.108734158709147
2	48	7.066342809565295
2	49	0.7102574427180208
2	50	7.353765034922574
2	51	9.662880593412758
2	52	9.008981570886927
2	53	9.227696689173563
2	54	9.54699386162627
2	55	10.387087453132153
2	56	10.746494368118956
3	1	3.6402645924634385
3	2	4.746578582848855
3	3	4.750838535706244
3	4	4.63399682258715
3	5	8.676226532221499
3	6	4.94277830738438
3	7	4.506484476080459
3	8	4.222393169102247
3	9	4.641515783088757
3	10	5.331720745487878
3	11	4.543514190561844
3	12	5.157003607647958
3	13	5.699179960677014
3	14	5.483783869039804
3	15	0.7761618039443903
3	16	6.181937011395288
3	17	7.527834191655472
3	18	7.816895598788385
3	19	6.545840970707005
3	20	5.513904599297998
3	21	7.159776705089897
3	22	7.575364690911421
3	23	6.381736799092135
3	24	6.985623989900535
3	25	7.097280339868863
3	26	4.536039012738023
3	27	5.37383287363112
3	28	5.073273560251167
3	29	5.427750700420589
3	30	5.424716278537598
3	31	4.9311644716030045
3	32	4.749705185272884
3	33	4.951142822928173
3	34	4.721380648376002
3	35	4.370342780096667
3	36	5.761579282938133
3	37	6.120007575814881
3	38	6.843247502167426
3	39	8.473646217901969
3	40	9.226306588182467
3	41	11.588537194007053
3	42	10.376239400614903
3	43	11.046030231384641
3	44	11.198612340132357
3	45	10.489246967434116
3	46	11.222844323697142
3	47	12.307419699659597
3	48	12.71902228378168
3	49	13.218346421484448
3	50	12.26225846262733
3	51	11.60170769149163
3	52	11.170761146215543
3	53	12.30555310980046
3	54	12.863980717035776
3	55	11.150990385386951
3	56	10.943663225550704
4	1	3.328119889621057
4	2	3.746578582848855
4	3	4.056781688161024
4	4	3.7905859698739732
4	5	7.720670976665943
4	6	4.209444974051046
4	7	4.070308765486146
4	8	3.770196786673303
4	9	3.4869938192644696
4	10	3.8552349573741864
4	11	2.993643389528252
4	12	5.0319390081647555
4	13	4.951893138971592
4	14	5.483783869039804
4	15	5.727582992574881
4	16	6.72663985377255
4	17	8.020857447469425
4	18	8.361598441165647
4	19	7.3189675856940895
4	20	6.7102870282385645
4	21	6.62747696348783
4	22	7.097845311066457
4	23	6.346077884363449
4	24	7.361334584215786
4	25	6.7107170323753245
4	26	5.673506712996421
4	27	4.610008584225437
4	28	4.8076404853157655
4	29	5.247388943314647
4	30	5.920323513679715
4	31	4.574575324316179
4	32	4.90267676150027
4	33	4.320135070990194
4	34	5.08158736672225
4	35	3.9765443304842627
4	36	3.9481425904316687
4	37	4.109671658502222
4	38	5.3672785099193625
4	39	5.112922703690078
4	40	-1.6729182180190811
4	41	9.819544945945033
4	42	9.325076609917229
4	43	8.600035399343298
4	44	9.655459885351995
4	45	9.380203039785531
4	46	12.395970938684226
4	47	12.260908071752624
4	48	12.044603679130514
4	49	11.205426524843624
4	50	11.287581460043349
4	51	9.650803298726768
4	52	10.334585435621229
4	53	10.000643549076944
4	54	11.270182267423369
4	55	11.060034313035533
4	56	8.920407411597218
5	1	3.7708549323561016
5	2	4.720937557207826
5	3	4.09609792747726
5	4	4.170073149361151
5	5	8.291611147606112
5	6	4.763291127897197
5	7	4.205351500528877
5	8	3.881307897784417
5	9	4.228874161144809
5	10	4.66207256421179
5	11	4.68424168012654
5	12	4.907152683378428
5	13	5.32112390820236
5	14	5.2786556639116
5	15	7.093394958386845
5	16	7.283904811037505
5	17	8.056754883366864
5	18	9.252196731763938
5	19	7.505292372018872
5	20	7.035073353024888
5	21	6.849699185710051
5	22	7.600409413630558
5	23	7.284539422824988
5	24	6.964753387634591
5	25	7.040631562289857
5	26	5.808549448039159
5	27	5.854453028669877
5	28	5.583708861384146
5	29	6.331149627075327
5	30	6.383571376927581
5	31	5.548934298675157
5	32	5.140283599107111
5	33	6.330391481246604
5	34	4.454236939371828
5	35	4.048339202279134
5	36	6.356689598978679
5	37	7.143859692690256
5	38	6.915996458637313
5	39	8.921469712237087
5	40	8.532209987109127
5	41	11.901596227996315
5	42	10.571230456071074
5	43	11.690633689941592
5	44	12.067425697317809
5	45	9.894732954315447
5	46	12.464347007060294
5	47	12.363472174316726
5	48	12.172808807335645
5	49	12.940469259886356
5	50	12.6397182121801
5	51	10.590974238897712
5	52	11.408089709125502
5	53	10.59893414736754
5	54	12.054797652038754
5	55	9.566017219018438
5	56	8.843484334674141
6	1	3.847778009279182
6	2	4.5414503777206505
6	3	4.865328696708033
6	4	4.811098790386794
6	5	8.958277814272783
6	6	5.070983435589511
6	7	4.564325859503235
6	8	4.214641231117746
6	9	4.8186177508884
6	10	5.354380256519484
6	11	5.120139116023978
6	12	6.291768067993814
6	13	6.551893138971586
6	14	6.124809510065447
6	15	7.221600086591977
6	16	7.694161221293914
6	17	8.877267703879681
6	18	9.636812116379318
6	19	8.428369295095798
6	20	7.163278481230019
6	21	7.798417134428
6	22	8.574768387989536
6	23	7.874283012568579
6	24	7.939112361993562
6	25	7.066272587930879
6	26	6.603421242910947
6	27	6.521119695336548
6	28	6.301657579332861
6	29	6.382431678357378
6	30	6.178443171799376
6	31	5.62585737559823
6	32	5.909514368337877
6	33	5.073981224836341
6	34	4.710647195782084
6	35	5.30474945868939
6	36	7.510535752824833
6	37	8.041295590126154
6	38	7.633945176586028
6	39	9.61377740454478
6	40	7.019389474288616
6	41	13.15800648440657
6	42	12.443025327865943
6	43	12.48550548481338
6	44	13.452041081933189
6	45	11.58704064662314
6	46	14.541270083983367
6	47	14.671164482009033
6	48	14.685629320156156
6	49	15.453289772706874
6	50	14.665359237821129
6	51	14.385846033769507
6	52	14.3055256065614
6	53	14.470729019162416
6	54	14.747105344346448
6	55	13.976273629274846
6	56	14.715279206469013
7	1	3.568228290705072
7	2	4.063651753580565
7	3	4.6345594659388
7	4	4.462130685321128
7	5	8.407933849294672
7	6	4.967168551286818
7	7	3.936433426732755
7	8	3.913828222987668
7	9	4.640381353139809
7	10	4.513229537319987
7	11	4.3665431185255414
7	12	4.196708655861229
7	13	5.8608362284024835
7	14	5.3618326495276065
7	15	6.048992206667023
7	16	7.364580233176344
7	17	7.669637935274302
7	18	8.950758332764558
7	19	7.526555661574847
7	20	6.981289738265662
7	21	6.475715445872652
7	22	7.294593278546131
7	23	7.0550209737943455
7	24	6.5976489473594135
7	25	6.082532750532504
7	26	4.824184219771489
7	27	5.529249776637361
7	28	4.852626935180268
7	29	5.625708726512478
7	30	6.466122971674299
7	31	5.3713232917958535
7	32	4.923272967462335
7	33	5.570541575055234
7	34	5.496763518483771
7	35	5.461097176012718
7	36	6.288521994225707
7	37	6.253303094816587
7	38	6.804676883903102
7	39	8.503708611549161
7	40	8.237650887671975
7	41	12.578894539440967
7	42	12.13971075625869
7	43	11.096512364112947
7	44	11.770365034403483
7	45	10.243075668511825
7	46	13.03282730724791
7	47	13.18773734004531
7	48	12.629969532789051
7	49	13.793502405602432
7	50	14.332025904487793
7	51	13.328310073794523
7	52	13.381197901745889
7	53	13.493868481326263
7	54	14.101076576366456
7	55	14.177649489187292
7	56	11.798456192085023
8	1	3.6901795102172628
8	2	4.746578582848855
8	3	4.829681417158312
8	4	4.730423368247955
8	5	8.237202141977598
8	6	4.918388063481942
8	7	4.521799280391292
8	8	4.377242857134007
8	9	4.737942328749561
8	10	5.659571000734616
8	11	4.488494338037739
8	12	5.757684265617328
8	13	5.787665496695169
8	14	5.800857039771515
8	15	6.6587483042279985
8	16	7.8523851112251215
8	17	8.596467203566988
8	18	8.36539247910602
8	19	8.331433710355334
8	20	7.078850713875422
8	21	7.085471543433634
8	22	7.611666449277841
8	23	7.44526487623337
8	24	8.573258703456975
8	25	7.594727872483727
8	26	5.311989097820266
8	27	4.895103435173947
8	28	4.925797666887583
8	29	5.991562385049065
8	30	5.978318093625521
8	31	4.51766475521049
8	32	4.557419308925748
8	33	4.570541575055234
8	34	3.7406659575081598
8	35	4.900121566256619
8	36	6.215351262518393
8	37	6.4240348021336615
8	38	7.072969566829929
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115	5	6.342893198888163
115	6	4.2390746036806775
115	7	3.8406791358565116
115	8	3.7331597496362647
115	9	3.5166234488941015
115	10	2.981160883300113
115	11	1.2454952413801053
115	12	-3.064357288131543
115	13	-2.559217972139521
115	14	-4.923623538367604
115	15	-6.576120711128823
115	16	3.3266398537725443
115	17	3.620857447469426
115	18	4.48011695968416
115	19	3.5708194375459357
115	20	3.451027768979305
115	21	5.960810296821165
115	22	5.794141607362754
115	23	6.375707513993081
115	24	6.494667917549119
115	25	5.9181244397827335
115	26	4.888321527811236
115	27	4.410008584225434
115	28	3.8002330779083593
115	29	4.425166721092424
115	30	4.40921240256861
115	31	4.685686435427293
115	32	4.450824909648421
115	33	5.549764700619821
115	34	4.881587366722254
115	35	4.880248034187968
115	36	6.570364812653896
115	37	7.109671658502222
115	38	6.85616739880825
115	39	7.357367148134525
115	40	9.141896596795732
115	41	9.249174575574663
115	42	8.058409943250563
115	43	6.88151688082478
115	44	-5.707503077610969
115	45	6.350573410155903
115	46	1.7293042720175578
115	47	-8.776128965284412
115	48	1.859418493945327
115	49	-0.12790680848971192
115	50	2.369062941524831
115	51	5.502655150578622
115	52	5.653103954139748
115	53	5.852495400928795
115	54	6.596108193349295
115	55	6.289663942665165
115	56	8.179666670856477
116	1	3.036363617219628
116	2	3.714320518332727
116	3	3.892623982067832
116	4	4.334671991379352
116	5	7.289129758027947
116	6	4.200842823513412
116	7	4.047369697385783
116	8	3.6124906934833376
116	9	3.761545790590631
116	10	4.546274383897483
116	11	3.2681953608544134
116	12	3.3107920547597374
116	13	4.304581311014601
116	14	4.67733225613658
116	15	-7.703958226063111
116	16	2.9538799971417227
116	17	4.685373576501682
116	18	4.717870842599339
116	19	5.296028517593726
116	20	3.44863828271885
116	21	5.731419615817579
116	22	6.186017354077208
116	23	4.85360476608388
116	24	6.842338168445178
116	25	6.044767211586798
116	26	5.661320333068105
116	27	5.187786362003212
116	28	4.257819696785305
116	29	4.2889660042465465
116	30	5.366201649880434
116	31	3.7143602705527385
116	32	4.546404360066578
116	33	4.4470167914203
116	34	4.422806004715081
116	35	5.187297018656306
116	36	5.362479507994397
116	37	4.274546210473552
116	38	4.569429047553772
116	39	8.196076825553877
116	40	9.348587158325003
116	41	6.866856773902025
116	42	5.692818545401099
116	43	1.4143723169060216
116	44	1.3809079140258298
116	45	5.342210208244314
116	46	1.5536770318741873
116	47	-7.997156444376408
116	48	0.302668195259546
116	49	-1.8304157690631868
116	50	2.267509775455533
116	51	6.141842725250065
116	52	5.324549593327323
116	53	3.832184767714935
116	54	4.422870439466379
116	55	5.1689948865122375
116	56	6.7591170890165735
117	1	2.5336754451766126
117	2	3.0799119161821906
117	3	3.3428927992721356
117	4	3.4072526365406404
117	5	5.759559865554834
117	6	3.7761116407177155
117	7	3.6091976543750306
117	8	3.1729745644510814
117	9	3.5397715970422468
117	10	4.069123846263075
117	11	0.046421167306029076
117	12	-10.365283214057467
117	13	-11.239773527695078
117	14	-2.7245494642935277
117	15	-10.858528118536231
117	16	-8.22891570178301
117	17	-1.379142552530574
117	18	-5.343957114389912
117	19	-0.9338101920836905
117	20	-1.234157416205882
117	21	3.474699185710051
117	22	4.956178644399792
117	23	3.371077884363448
117	24	4.064112361993562
117	25	4.816272587930879
117	26	5.462395601885312
117	27	4.062786362003212
117	28	4.679862707537993
117	29	4.911277832203531
117	30	5.450879069235274
117	31	4.157908657649514
117	32	4.441565650389162
117	33	4.545135070990188
117	34	4.492698477833361
117	35	4.051544330484258
117	36	6.375920368209446
117	37	6.387449436280001
117	38	4.5506118432526925
117	39	6.399033814801189
117	40	7.785415115314251
117	41	5.202878279278369
117	42	2.5584099432505596
117	43	-10.030520156212257
117	44	-2.2306512257591145
117	45	2.619091928674422
117	46	-2.465140172426885
117	47	-1.7807585949140439
117	48	0.419603679130514
117	49	-1.905684586267487
117	50	0.7486925711544608
117	51	5.7480255209489926
117	52	4.731807657843451
117	53	5.028421326854719
117	54	5.211848934090035
117	55	4.2711454241466456
117	56	6.2120740782638855
118	1	2.6170087785099483
118	2	2.5243563606266335
118	3	3.6345594659388
118	4	3.6016970809850832
118	5	6.39844875444372
118	6	3.109444974051044
118	7	3.081419876597252
118	8	3.1035301200066385
118	9	3.1092160414866896
118	10	3.499679401818625
118	11	1.2269767228615862
118	12	-10.101394325168577
118	13	-6.003662416583968
118	14	-4.793993908737974
118	15	-9.094639229647342
118	16	-6.284471257338563
118	17	1.620857447469426
118	18	-4.593957114389912
118	19	0.718967585694088
118	20	0.09917591712745377
118	21	6.12747696348783
118	22	5.7200675332886775
118	23	5.801633439919005
118	24	5.550223473104676
118	25	6.177383699041993
118	26	4.6290622685519764
118	27	3.521119695336548
118	28	3.652084929760214
118	29	3.2029444988702025
118	30	4.298101291457495
118	31	2.9079086576495143
118	32	3.358232317055826
118	33	2.920135070990188
118	34	2.5482540333889183
118	35	3.6765443304842584
118	36	4.903698145987224
118	37	5.220782769613336
118	38	5.7450562876971425
118	39	6.024033814801189
118	40	8.215970670869808
118	41	7.341767168167259
118	42	5.613965498806117
118	43	-1.5999646006567012
118	44	4.533237663129775
118	45	5.813536373118868
118	46	2.0070820497953363
118	47	2.1497969606415133
118	48	3.1557147902416247
118	49	2.8165376359547345
118	50	2.7764703489322358
118	51	8.428581076504546
118	52	9.13458543562123
118	53	5.111754660188055
118	54	8.781293378534482
118	55	8.215589868591088
118	56	10.86485185604166
119	1	2.1822261698142995
119	2	3.0074481480662456
119	3	3.1562985963735812
119	4	3.092035245236289
119	5	5.632748271351936
119	6	3.3775609160800286
119	7	2.779487509447499
119	8	3.1131919557554326
119	9	3.3169455100857235
119	10	3.5673122520601765
119	11	1.1279429064364663
119	12	-8.874341185071959
119	13	-2.8539039624776876
119	14	-5.820563957047153
119	15	-8.751644060565216
119	16	-5.562249035116341
119	17	-0.37914255253057405
119	18	-4.144681752071069
119	19	-0.9283754094749952
119	20	-1.371838575626171
119	21	5.009119475565129
119	22	5.483352557443268
119	23	5.253324261175042
119	24	4.765199318515304
119	25	5.3996059212642145
119	26	4.9768883555085
119	27	5.013873318524954
119	28	4.226964156813352
119	29	5.478306817710781
119	30	5.119357330104837
119	31	4.342691266345163
119	32	3.6698265199543805
119	33	4.5143379695409145
119	34	3.891249202471048
119	35	5.162051576861071
119	36	6.241862397194954
119	37	5.887449436280001
119	38	5.938293002672982
119	39	7.6617149742214785
119	40	7.950270187778024
119	41	6.329689873481268
119	42	3.594641827308532
119	43	-1.3584187069368951
119	44	3.91246471626987
119	45	4.274889030123699
119	46	0.31384533481949006
119	47	0.260908071752624
119	48	-5.303222407826009
119	49	0.7319965731528022
119	50	2.375504165357359
119	51	7.467228419499715
119	52	7.09593809262606
119	53	6.227696689173563
119	54	6.1339503833653986
119	55	6.234913540088673
119	56	8.789972628988522
120	1	2.6170087785099483
120	2	4.064760401030675
120	3	3.770923102302433
120	4	3.9754344547224605
120	5	7.448953804948772
120	6	3.715505580111653
120	7	4.010712805890186
120	8	3.775247291723808
120	9	3.8920443243149734
120	10	3.656245058384286
120	11	0.3986938945787557
120	12	-6.7478589716332245
120	13	-3.751137164058715
120	14	-4.061670676414742
120	15	-0.8623159973241101
120	16	-5.9713399442072514
120	17	1.2572210838330626
120	18	-1.3363813568141545
120	19	-0.6951738284473272
120	20	0.7506910686426025
120	21	3.2739416099524803
120	22	5.240269553490698
120	23	4.98850212678769
120	24	3.9845669074481087
120	25	3.7177877394460346
120	26	5.856334995824703
120	27	4.505968180185032
120	28	4.596529374204657
120	29	5.096883892809593
120	30	6.6667881601443675
120	31	5.271545021285881
120	32	4.964292923116435
120	33	2.708013858868977
120	34	4.5533045384394235
120	35	3.555332209272141
120	36	4.868344610633688
120	37	4.614722163552727
120	38	5.133945176586028
120	39	6.1300944208617985
120	40	8.13011208501122
120	41	-4.941061114661025
120	42	-1.9112870264464092
120	43	-6.716126216818317
120	44	-14.704136074243964
120	45	-4.736968677386184
120	46	-10.199988657275371
120	47	-7.193637382792831
120	48	-6.319032684505849
120	49	-13.617805798388702
120	50	-3.9407013682394805
120	51	0.2063588542823247
120	52	1.4477167487525406
120	53	0.854178902612297
120	54	1.9580610553021565
120	55	-0.031884878883658985
120	56	3.4658619570517644
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	566.0
5	15.0
6	26.0
7	66.0
8	93.0
9	135.0
10	84.0
11	45.0
12	61.0
13	55.0
14	79.0
15	75.0
16	111.0
17	129.0
18	163.0
19	264.0
20	311.0
21	427.0
22	507.0
23	639.0
24	739.0
25	785.0
26	902.0
27	921.0
28	965.0
29	1041.0
30	1102.0
31	1270.0
32	1391.0
33	1630.0
34	1706.0
35	1858.0
36	2096.0
37	2338.0
38	2124.0
39	1615.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.24812030075188	0.6379585326953748	0.06455532771322245	0.04936583883952305
8	99.58988380041012	0.27720817194501407	0.09113693324219639	0.04177109440267335
9	98.86838307890939	0.3113845219108377	0.40252145515303417	0.4177109440267335
10	50.455684666210985	26.706918812181968	14.09204830257462	8.74534821903243
11	33.77762588288904	25.02088554720134	19.01724006987165	22.184248500037974
12	28.761297182349814	18.47041847041847	21.705779600516443	31.062504746715273
13	23.809523809523807	13.868003341687551	25.005696058327636	37.316776790461006
14	22.704488493962177	26.741095162147793	26.13351560719982	24.42090073669021
15	30.201419089036392	20.19150072480354	24.17792019531548	25.429159990844585
16	31.73131719919456	24.698909615896053	21.959651988906195	21.610121196003192
17	27.794447187511867	18.994264879030727	32.40913061643055	20.802157317026854
18	22.79182805498595	19.305840358471936	28.203083466241363	29.69924812030075
19	29.11695417584923	21.661980393646935	20.31309370012919	28.907971730374648
20	26.98406662356923	23.66809902270221	26.89660417538122	22.45123017834734
21	25.23353839143313	19.936204146730464	27.492974861395915	27.337282600440492
22	22.442469810890863	18.81597934229513	31.12326270221007	27.618288144603937
23	20.946305156831475	22.514619883040936	27.363864205969467	29.175210754158122
24	20.62352851826536	21.565276828434722	27.713222450064556	30.09797220323536
25	27.224395245509434	24.930695325257282	25.059810883682072	22.78509854555121
26	31.752906314109868	22.17536661347922	20.33280145885571	25.738925613555203
27	22.593394899859383	26.27978565727967	21.346862767453352	29.779956675407593
28	25.491759702286014	25.3816359079517	23.752563226247435	25.37404116351485
29	31.536910964895597	23.504354771231885	20.290571635035942	24.668162628836573
30	28.07964131012995	20.974238163994226	28.524203966866786	22.42191655900904
31	28.727687048993545	27.341435624762628	22.495252563615647	21.43562476262818
32	32.68646513747532	24.137931034482758	21.7340118487012	21.44159197934073
33	28.443261737312298	24.10948488880441	22.471013115377307	24.97624025850599
34	24.06392694063927	26.088280060882802	29.901065449010655	19.946727549467276
35	23.817310617584422	22.51292972315181	32.67797383632492	20.991785822938848
36	28.072641616959842	24.774894570874967	25.671517039626153	21.480946772539035
37	26.844386979008217	21.524186188013385	29.38469729236386	22.24672954061454
38	31.553508905465062	24.96955396559598	22.769827979905617	20.70710914903334
39	28.115015974440894	22.223490034991634	21.873573710634414	27.787920279933058
40	23.142616421568626	17.631740196078432	25.938265931372552	33.287377450980394
41	20.759571445028993	15.168388310740754	22.73338197457855	41.3386582696517
42	25.12187690432663	26.35588056063376	22.73765996343693	25.784582571602684
43	19.645168659103025	20.368537272519607	30.26345846341278	29.722835604964594
44	22.795575896262395	23.699466056445463	23.901601830663616	29.60335621662853
45	26.71436182357866	21.413349569982497	28.567623106781337	23.30466549965751
46	21.232433255893664	26.031153597136004	25.471302890657732	27.265110256312603
47	18.19603952840627	23.835323743752145	25.38440993551833	32.58422679232324
48	24.232522796352583	24.198328267477205	24.870820668693007	26.698328267477205
49	20.986290936785988	25.853008377760855	22.955064737242957	30.205635948210208
50	24.91344214891755	24.3008788951033	26.321196210478252	24.46448274550089
51	22.539718825008574	25.107631348344572	28.136548938926353	24.2161008877205
52	22.40566037735849	27.172093730979913	27.849208764455263	22.57303712720633
53	23.23774705815149	25.290376632773526	27.11832133744621	24.353554971628775
54	23.523367331405083	23.96864058456386	27.355761912010962	25.152230172020097
55	20.687816582244732	24.87717561031344	28.560003046806564	25.87500476063526
56	24.574626013474933	20.85569639526474	29.172852194434927	25.396825396825395
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	2.0
10	5.0
11	8.0
12	15.0
13	31.5
14	41.0
15	49.5
16	58.0
17	60.0
18	62.0
19	52.0
20	42.0
21	51.0
22	61.5
23	63.0
24	69.0
25	75.0
26	82.0
27	89.0
28	96.5
29	108.0
30	112.0
31	132.5
32	153.0
33	193.0
34	233.0
35	261.0
36	289.0
37	315.5
38	364.5
39	387.0
40	434.5
41	482.0
42	545.5
43	609.0
44	892.5
45	1176.0
46	1672.0
47	2057.5
48	1947.0
49	1691.5
50	1436.0
51	1503.5
52	1571.0
53	1571.5
54	1740.5
55	1909.0
56	1773.5
57	1638.0
58	1662.0
59	1686.0
60	1709.0
61	1732.0
62	1655.5
63	1380.0
64	1181.0
65	1092.5
66	1004.0
67	807.0
68	610.0
69	599.5
70	589.0
71	510.0
72	397.5
73	364.0
74	272.5
75	181.0
76	135.5
77	90.0
78	86.0
79	97.5
80	113.0
81	72.0
82	31.0
83	19.0
84	7.0
85	5.5
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.45568466621098197
16	0.04936583883952305
17	0.01898686109212425
18	0.0
19	0.06075795549479761
20	0.14050277208171943
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0037973722184248505
26	0.045568466621098196
27	0.07974481658692185
28	0.0
29	0.15569226095541885
30	0.06075795549479761
31	0.015189488873699402
32	0.007594744436849701
33	0.11012379433432064
34	0.2050580997949419
35	0.1443001443001443
36	0.04936583883952305
37	0.1443001443001443
38	0.22024758866864128
39	0.1594896331738437
40	0.8430166324903167
41	1.112630059998481
42	0.2961950330371383
43	0.2582213108528898
44	0.4329004329004329
45	0.21265284423179162
46	0.29239766081871343
47	0.47467152730310624
48	0.053163211057947905
49	0.28100554416343887
50	0.19366598313966735
51	0.3303713830029619
52	0.1746791220475431
53	0.2848029163818638
54	0.22024758866864128
55	0.29239766081871343
56	0.23923444976076555
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	26334.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.2740943267259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.0599087893864	65.25784157363105
2	6.7837893864013274	9.941520467836257
3	1.6998341625207296	3.7366142629300527
4	0.7462686567164178	2.1872863978127137
5	0.3990464344941957	1.461988304093567
6	0.2798507462686567	1.2303485987696514
7	0.18656716417910446	0.9569377990430622
8	0.11401326699834163	0.6683375104427736
9	0.0881011608623549	0.5809979494190021
>10	0.5700663349917081	7.9630895420369106
>50	0.04145936981757877	1.9062808536492746
>100	0.03109452736318408	4.1087567403356875
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	346	1.313890787574998	No Hit
TGTCACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	192	0.7290954659375711	No Hit
TGTCACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	170	0.6455532771322244	No Hit
TGTCACGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	149	0.5658084605453026	No Hit
TGTCACGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	114	0.4329004329004329	No Hit
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	111	0.42150831624515833	No Hit
TGTCACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	80	0.303789777473988	No Hit
TGTCACGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	73	0.27720817194501407	No Hit
TGTCACGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC	63	0.23923444976076555	No Hit
TGTCACGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	63	0.23923444976076555	No Hit
TGTCACGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	59	0.22404496088706616	No Hit
TGTCACGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT	58	0.22024758866864128	No Hit
TGTCACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	55	0.20885547201336674	No Hit
TGTCACGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	51	0.19366598313966735	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	50	0.1898686109212425	No Hit
TGTCACGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG	49	0.18607123870281764	No Hit
TGTCACGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT	47	0.17847649426596796	No Hit
TGTCACGGGAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGA	47	0.17847649426596796	No Hit
TGTCACGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	46	0.1746791220475431	No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	42	0.1594896331738437	No Hit
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	41	0.15569226095541885	No Hit
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	41	0.15569226095541885	No Hit
TGTCACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	38	0.1443001443001443	No Hit
TGTCACGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTCATGGTTCCTTTGGTC	37	0.14050277208171943	No Hit
TGTCACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	36	0.1367053998632946	No Hit
TGTCACGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG	35	0.13290802764486975	No Hit
TGTCACGGGACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGG	34	0.1291106554264449	No Hit
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	33	0.12531328320802004	No Hit
TGTCACGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	33	0.12531328320802004	No Hit
TGTCACGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	33	0.12531328320802004	No Hit
TGTCACGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC	32	0.12151591098959522	No Hit
TGTCACGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG	31	0.11771853877117035	No Hit
TGTCACGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG	30	0.11392116655274549	No Hit
TGTCACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC	30	0.11392116655274549	No Hit
TGTCACGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA	30	0.11392116655274549	No Hit
TGTCACGGGGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACAT	28	0.10632642211589581	No Hit
TGTCACGGGCATTACCTTTATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAG	28	0.10632642211589581	No Hit
TGTCACGGGAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG	28	0.10632642211589581	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.00379737221842485	0.0	0.0	0.0	0.0
12	0.00379737221842485	0.0	0.0	0.0	0.0
13	0.01139211665527455	0.0	0.0	0.0	0.0
14	0.0455684666210982	0.0	0.0	0.0	0.0
15	0.0531632110579479	0.0	0.0	0.0	0.0
16	0.0607579554947976	0.0	0.0	0.0	0.0
17	0.0683526999316473	0.0	0.0	0.0	0.0
18	0.0683526999316473	0.0	0.0	0.0	0.0
19	0.07215007215007214	0.0	0.0	0.0	0.0
20	0.07215007215007214	0.0	0.0	0.0	0.0
21	0.075947444368497	0.0	0.0	0.0	0.0
22	0.075947444368497	0.0	0.0	0.0	0.0
23	0.07974481658692185	0.0	0.0	0.0	0.0
24	0.0835421888053467	0.0	0.0	0.0	0.0
25	0.0911369332421964	0.0	0.0	0.0	0.0
26	0.0911369332421964	0.0	0.0	0.0	0.0
27	0.0911369332421964	0.0	0.0	0.0	0.0
28	0.0987316776790461	0.0	0.0	0.0	0.0
29	0.10252904989747096	0.0	0.0	0.0	0.0
30	0.1063264221158958	0.0	0.0	0.0	0.0
31	0.1139211665527455	0.0	0.0	0.0	0.0
32	0.11771853877117035	0.0	0.0	0.0	0.0
33	0.1215159109895952	0.0	0.0	0.0	0.0
34	0.1215159109895952	0.0	0.0	0.0	0.0
35	0.12531328320802004	0.0	0.0	0.0	0.0
36	0.1291106554264449	0.0	0.0	0.0	0.0
37	0.1291106554264449	0.0	0.0	0.0	0.0
38	0.1291106554264449	0.0	0.0	0.0	0.0
39	0.1291106554264449	0.0	0.0	0.0	0.0
40	0.1291106554264449	0.0	0.0	0.0	0.0
41	0.1291106554264449	0.0	0.0	0.0	0.0
42	0.1443001443001443	0.0	0.0	0.0	0.0
43	0.14809751651856914	0.0	0.0	0.0	0.0
44	0.1670843776106934	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGGA	20	3.7552457E-4	50.916183	38
CTCGGAT	20	3.7552457E-4	50.916183	39
GCCCACG	15	0.0077604507	50.91618	38
TCGGATA	15	0.0077604507	50.91618	40
CCCACGG	15	0.0077604507	50.91618	39
CGGATAA	15	0.0077604507	50.91618	41
TACTCGG	20	3.8653033E-4	50.62016	37
TGCCCAC	15	0.007941937	50.620155	37
ATGCCCA	15	0.0080646705	50.42471	36
CATGCCC	15	0.0080646705	50.42471	35
TCCCAGG	15	0.008188809	50.23077	15
GACCCAC	20	4.0931057E-4	50.03832	9
GAAGCCA	15	0.008314364	50.038315	9
GATTGGA	15	0.008314364	50.038315	9
GACGCAC	15	0.008314364	50.038315	9
GATCCAA	15	0.008314364	50.038315	9
GCTCCTA	15	0.008314364	50.038315	9
TCAGCGG	20	4.2109375E-4	49.752384	27
CTCAGCG	20	4.2109375E-4	49.752384	26
CAGCGGT	20	4.2109375E-4	49.752384	28
>>END_MODULE