FastQCFastQC Report
Fri 17 Jun 2016
SRR1525884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107
Sequences flagged as poor quality0
Sequence length40
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGCCCTGGTATCCCCCANCANANNAACACAACA10.9345794392523363No Hit
TGTCACGGGCTGATTTTCTTCCTTGAGACTCACAGTGCCT10.9345794392523363No Hit
TGTCACGCCCGCCACCCCAGACCCCACGTCACCCACCACT10.9345794392523363No Hit
TGTCACGGGTTATCACCCCCGAGCCCACTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGTGGCCAGGTCGCCTTTTTTGGGTTTGTTTCG10.9345794392523363No Hit
TGTCACGGGCTCCCCCCCTTTTTTTTTTTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGACTTGATACAGTGCATACTGCCCCCCAAAAA10.9345794392523363No Hit
TGTCACGGGAACCTAGCCCGAACCCAAGCGCACCCTCATC10.9345794392523363No Hit
TGTCACGGGCTCCTTAACCCCCCTTTTCAAATGTGGAAAG10.9345794392523363No Hit
TGTCACGGGGCCGTGTCCTTAATCTTAATGTGGTCTCCCT10.9345794392523363No Hit
TGTCACGGGGGGGCTGGAGAGCTGCCTCCGCGGGTAAGAG10.9345794392523363No Hit
TGTCACGGGTTCCGTTTTTTTTTTTTTTTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGGTGCTTGTCCCGGGGGAGAAGCGGCGGCGCC10.9345794392523363No Hit
TGTCACGGGGCTTGTCCCGGGGGCCCGAGGGCCCTTGCGC10.9345794392523363No Hit
TGTCACGGGGCCTCGATTTTTTTTTTTTTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGCCGTGGTTCCCCCCCCGCCGCCTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGCCATGGTATCCCCCGCGCCGCCTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGCTGCTTCGCGAGTCGCCTTCTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGCAGCATGGTATCAACCCCGCGTCGCCTTTTT10.9345794392523363No Hit
TGTCACGGGAGGGTTTTACTATGTTGATCTAAGGTCTCCT10.9345794392523363No Hit
TGTCACGGGCCCTGCCCCCCCCCCCCCCCCCACCCACCAA10.9345794392523363No Hit
TGTCACGGGCACCAGCCAGGACAACAGACCGGAAGAGCTC10.9345794392523363No Hit
TGTCACGGGGGGGTTGGGGCTTTACCTCCCTGGCAGCCCC10.9345794392523363No Hit
TGTCACGGGCTCTGCGGTGAGGGGATCATGTAGCTTGGCT10.9345794392523363No Hit
TGTCACGGGTGCCAGAGCGACCTTTTTTTTTCTTTTTTTT10.9345794392523363No Hit
TGTCACGGGGTCCTTTTTTTTTTTTTTTTTTTTTTCTCCC10.9345794392523363No Hit
TGTCACGGGGGGTCTGCCTCTTGGAACAATTAATACAAGT10.9345794392523363No Hit
TGTCACGGCTCAAACTAAAACTATTTGTTATATATTTGAA10.9345794392523363No Hit
TGTCACGGGCGGTCTACCCCCTTACCCCCCCCCCTTTCCC10.9345794392523363No Hit
TGTCACGGGAGCCCTGACCTATGACCCCCGCCCGATTGAT10.9345794392523363No Hit
TGTCACGGGACTATTGACCTCCCGACTCCCACCACAAATG10.9345794392523363No Hit
TGTCACGGGGCCGTGCTTGTCCCGGGCTTGTTCTTCCCTC10.9345794392523363No Hit
TGTCACGGGGAGTGTTTGGGTTTCCCCCCACCCCCCCCCG10.9345794392523363No Hit
TGTCACGGGAACAGTTCAACCCAGAGTCGACTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGGGGCCGGGGTCTTTGTGAATGTGGCCCTTGT10.9345794392523363No Hit
TGTCACGGGCTCGATTTTTTTTTTTTTTTTTCCACACCAC10.9345794392523363No Hit
TGTCACGGGGCTTCTCTGCCCCCCCCGCCCCCCTGGCCCC10.9345794392523363No Hit
TGTCACGGGATCGCTTTTTTTTTTTTTTTTTCCCAACAAA10.9345794392523363No Hit
TGTCACGGGCCCTGGGGTTGCCCCCTCCTGTTTCTTTTTA10.9345794392523363No Hit
TGTCACGGGAAGCATGTTCAACCCCGCGTCGCCTTTTTTT10.9345794392523363No Hit
TGTCACGGGAGGCATTCAGTTAAAAGCATGCTGCCTAGTC10.9345794392523363No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCCGCGGTAGACCC10.9345794392523363No Hit
TGTCACGGGAGCAGTCTCAACCGCCCCCCTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGGGTTTGTTTACCTTGGAGCGCCGGGTGGGGT10.9345794392523363No Hit
TGTCACGGCTCCCCCCCAGCGCCCCCTCTTCTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGTCCCCGGGGCTGAGCCCTCAAACCCCACCCT10.9345794392523363No Hit
TGTCACGTGACGCCTGCTCACATGAACCCGTCCGGTTTGA10.9345794392523363No Hit
TGTCACGGGGGTATTTCTATCCGCCCAGCCCGCCCCTCCC10.9345794392523363No Hit
TGTCACGGGGCGTTGCCCAGCCTGTCCCCGGACCCGCCCC10.9345794392523363No Hit
TGTCACGGGAAGCAGTGTATCAACCCAGCGCCCCCCCTTT10.9345794392523363No Hit
TGTCACGGGAACAGTGGCCTAACCCCCACAACCACAAACA10.9345794392523363No Hit
TGTCACCGGGCGTCCCCTTTTTTTTTTTTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGGGCCCTCTTGGTTGGGTGGCTTTCCCCCTCT10.9345794392523363No Hit
TGTCACGGGTCAAGGTTTCCTTGTAACCCAAACACTGCCT10.9345794392523363No Hit
TGTCACGGGCTCCCGTGTCGACTTTTTTTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGGGGAATTCGCGGAGAAAGAAATGGCTGATCG10.9345794392523363No Hit
TGTCACGGGGGTTTGTTTTCCAGGAGCCTGGCTGGCCTTC10.9345794392523363No Hit
TGTCACGGGGTAAGGAATACCTCAGCAAAACATCCTCCCT10.9345794392523363No Hit
TGTCACGGGGTTTTTCAGGCCGCTTTGCAAGGGTAGCTGG10.9345794392523363No Hit
TGTCACGGGTGGCCACGCCGAGTCGACTTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGCTGTCCCGCTCGACTTTTTTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGAAGAGTGGCTAACCCCCCGCCGATTTTTTTT10.9345794392523363No Hit
TGTCACGGGAACAGTGGTATCAACCCCGCTCGCCTTTTTT10.9345794392523363No Hit
TGTCACGGGGTGTGGTCCCCGTTTCCTCAGTCCTTTCCTG10.9345794392523363No Hit
TGTCACGGGAGGGCTGTTGGGAATTGAGAAACCCTGTCTC10.9345794392523363No Hit
TGTCACGGGAAGCAGTGTCTCAACCCAACCCCCCCCAACA10.9345794392523363No Hit
TGTCACGGGGCTCCTCCGCTCTCCTCNGCCCCCCCGGCCG10.9345794392523363No Hit
TGTCACGGGGGCTTCCCTGGCCTGTATATCTCTTCCGCTC10.9345794392523363No Hit
TGTCACGGGAAGCATGTTCAACCCCAAGCCGACTTTCTTT10.9345794392523363No Hit
TGTCACGGGAAGCATGTACCCCCAGAGTCGAGGAAGAGCT10.9345794392523363No Hit
TGTCACGGGGAGCTTTGCGATTGCCCCCCCCCGTGTGCCG10.9345794392523363No Hit
TGTCACGGGGCCTGTCCCTCTCCGAGGTAATATCGGGGTA10.9345794392523363No Hit
TGTCACGGGGGTGGAGACAGAACCCGGGCCCACCAGCTTC10.9345794392523363No Hit
TGTCACGGGTCCGACGTCGACTTTTTTTTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGGCGACGGCCCTTCCCCTACCCTTCTCCCCTC10.9345794392523363No Hit
TGTCACGGGGGGAATCCCTGGAGAAAGACATGCCTCCCCT10.9345794392523363No Hit
TGTCACCCCCCCTTCCCCGCCCCCCTTAGATTTGTGATTT10.9345794392523363No Hit
TGTCACGGGCTGTTGGCCGTTGCCTTTTCATGTTTACCTT10.9345794392523363No Hit
TGTCACGGGATGTATCACGAGAGCCGACTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGTGGTTCCCCCCGAGCCCATTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGTTAACCAGAGTCCCCTTTTTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGCAGCCCCTAGAGAACACATTAAGTACAACAC10.9345794392523363No Hit
TGTCACGGGACCACTGTCAACCCCGACCCGACTTTTTTTT10.9345794392523363No Hit
TGTCACGGGTCCCTGCTTCACCCCCCCCCTACCCCATCCT10.9345794392523363No Hit
TGTCACGGGGTCTGTTTGCCGTCTTCTGCTTGCTCGGCAG10.9345794392523363No Hit
TGTCACGGGGTGTGGTCCCAGTTTACCCAGTGCTTTCATG10.9345794392523363No Hit
TGTCACGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTCC10.9345794392523363No Hit
TGTCACGGGACGCCTCGTCCCCACCCACCGTTTTCTTTTT10.9345794392523363No Hit
TGTCACGGGTCCCTGGTTGATCCCCCCAGTGGCCTATGCT10.9345794392523363No Hit
TGTCACGGGGGTCCGTTGTGGAGAGCCTGTAGCCGAGTTG10.9345794392523363No Hit
TGTCACGGGCCTGTCTCCCCCCCTGGACTGTCTCCCTCTG10.9345794392523363No Hit
TGTCACGGGGTACTTATCCCCGGGGGCAACTATTCCTTCC10.9345794392523363No Hit
TGTCACGGGAGGTATTCAGTTAAAACCACGCTGCCTAGTC10.9345794392523363No Hit
TGTCACGGGAGAGCCAACCCCCCGGCCCCGGCCCTTGTCC10.9345794392523363No Hit
TGTCACGGGTACCTGGTTGATCCAGATCGGAAGACCCCGT10.9345794392523363No Hit
TGTCACGGGGCCAACACAATCAGAATAACCAGCAGGTATC10.9345794392523363No Hit
TGTCACGGGACAGCAAATGAAGAACTGGTGCACCCAGAGC10.9345794392523363No Hit
TGTCACGGATTCCAGTGTCCCCTTTCTTTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGGGGTAACTATATTCCCATCCCCATGATGTTT10.9345794392523363No Hit
TGTCACGGGGGATACAGAGTCGACTTTTTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGGAGTTGGGAGGGCTTGGAGATCCCCCCTTGC10.9345794392523363No Hit
TGTCACGGGAAGCATGGTACAACCCAAACAAAAAACAACA10.9345794392523363No Hit
TGTCACGGGAGTGCAGTGCTTGCCCCGGGCCCGGTAGTGG10.9345794392523363No Hit
TGTCACGGTGTATGAAATGCTGCAGATCCCGAGTTTTTGA10.9345794392523363No Hit
TGTCACGGGGGCTACCGCGTCGCCTTTTTTTTTTTTTTTT10.9345794392523363No Hit
TGTCACGGGGCAGTGCTTGTCCCGGGATTGTTCTTCCATC10.9345794392523363No Hit
TGTCACGGGCCTTCTCTTCGGCAAAAACAACACCCACCAC10.9345794392523363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG100.034.02
TGTCACG100.034.01
TCACGGG100.034.03
Produced by FastQC (version 0.11.5)