FastQCFastQC Report
Fri 17 Jun 2016
SRR1525883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT201.3315579227696404No Hit
TGTCACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT120.7989347536617843No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC80.5326231691078562No Hit
TGTCACGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT70.4660452729693742No Hit
TGTCACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG60.3994673768308922No Hit
TGTCACGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT50.3328894806924101No Hit
TGTCACGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG40.2663115845539281No Hit
TGTCACGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT40.2663115845539281No Hit
TGTCACGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCT40.2663115845539281No Hit
TGTCACGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGA30.1997336884154461No Hit
TGTCACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA30.1997336884154461No Hit
TGTCACGGGGGGGAGGTAGTGACGAAAAATAACAATACAG30.1997336884154461No Hit
TGTCACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1997336884154461No Hit
TGTCACGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTA30.1997336884154461No Hit
TGTCACGGGGTTGTTGTTTTTGTTTTTAACTTGCAAGCCA30.1997336884154461No Hit
TGTCACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT30.1997336884154461No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG30.1997336884154461No Hit
TGTCACGGGTCACAGATCCCCTTCTGAAGGAGATTGGGCG20.13315579227696406No Hit
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC20.13315579227696406No Hit
TGTCACGGGATAGTTGTTCTTTCATCACGATCTTATGGCG20.13315579227696406No Hit
TGTCACGGGGAGGGGTTGGAGAACTGGCTTCGCGGTGTGT20.13315579227696406No Hit
TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA20.13315579227696406No Hit
TGTCACGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGC20.13315579227696406No Hit
TGTCACGGGCGACCTACCTGGAATAACGAAAGTGCCCGTG20.13315579227696406No Hit
TGTCACGGGACTAATCCTAGCCCTAGCCCTACACAAATAT20.13315579227696406No Hit
TGTCACGGGGCTGCGCTCCAATTGCGACTCTAGCCTTTTC20.13315579227696406No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAAAC20.13315579227696406No Hit
TGTCACGGGAGGGTTGTTCTGTGTAGCTTTGGCTCTCCTA20.13315579227696406No Hit
TGTCACGGGGTTTTTTAATGTTTCATGACTCCCACTTTGA20.13315579227696406No Hit
TGTCACGGGATGTTTAAGTTTTCTGGGTTACTGGATTTCA20.13315579227696406No Hit
TGTCACGGGGCCAAGGCTGAGGAAGATCAAGCATGCCCGG20.13315579227696406No Hit
TGTCACGGGACTTTAAATCCTTTAACGAGGATCCATTGGA20.13315579227696406No Hit
TGTCACGGGGGAGGGGTGTATATTTTGATAAAATACTTGA20.13315579227696406No Hit
TGTCACGGGCTACAGCTTTACCACCACTGCTGAGAGGGAG20.13315579227696406No Hit
TGTCACGGGGAAGCTAGCTTGCATTCTCCCCTCATTCATC20.13315579227696406No Hit
TGTCACGGGAGCATTTTTTACTTTCAATGCCTTAGCTAGC20.13315579227696406No Hit
TGTCACGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT20.13315579227696406No Hit
TGTCACGGGGGTCGGGCGCCGGCGGCTTTGGTGACTCTAG20.13315579227696406No Hit
TGTCACGGGGGGCACCATGGTTCTGACCAATTTGACAGCT20.13315579227696406No Hit
TGTCACGGGTCATAGATCCTCTTCTGAGATGAGTTTTTGT20.13315579227696406No Hit
TGTCACGGGGGCTATATAGGAATAGTATGAGCAATAATGT20.13315579227696406No Hit
TGTCACGGGAGCTGGTGCCATGGATGTTCCTCTGACTTAG20.13315579227696406No Hit
TGTCACGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT20.13315579227696406No Hit
TGTCACGGGGTTTGTTTTGTGTTTTGAGGTAGGGTCTCTA20.13315579227696406No Hit
TGTCACGGGATCCCAAATACTGGTTAACTCCCCCGGCCTT20.13315579227696406No Hit
TGTCACGGGGTGTTACTTGACCTTTTTCCCTTTCTGCTTT20.13315579227696406No Hit
TGTCACGGGAGGAAGTATACTGTGTGCTTTTATATAACCA20.13315579227696406No Hit
TGTCACGGGTGTGCGCTCCGTGTTAGTGTAGCCCTCCACG20.13315579227696406No Hit
TGTCACGGGGACTTTGAGAAACTCTGCTCCATTTCTCTCT20.13315579227696406No Hit
TGTCACGGGGTTCTATCAATGGGAGCAGTGTTTGCTATCA20.13315579227696406No Hit
TGTCACGGGGGGTGCTGGAATTCTAGGCCTGCATGGCCTC20.13315579227696406No Hit
TGTCACGGGGTTATAAATTAACTTATTTCTAGATTGTTTT20.13315579227696406No Hit
TGTCACGGGGTGCTCTCCTCGCCTGATCATGACCATGCAC20.13315579227696406No Hit
TGTCACGGGCCCTGGACCTTTTGGATTTGAGATTACTAGG20.13315579227696406No Hit
TGTCACGGGCATTACCTTTATGTTACTTTATGCTGCTGGC20.13315579227696406No Hit
TGTCACGGGCTCCTGTGTAGCCCTGTGGTGCAATGCGACG20.13315579227696406No Hit
TGTCACGGGGGAATTGTTTTATCCAACTCATCCCTTGACA20.13315579227696406No Hit
TGTCACGGGTGACTTAGGAAGTTTCCAGGTAGACTTGGAG20.13315579227696406No Hit
TGTCACGGGGTTTGAAAATGTCAGGTCTTCTGCCTCTTGT20.13315579227696406No Hit
TGTCACGGGACCCGGGGAGGTAGTGACGAAAAATAACAAT20.13315579227696406No Hit
TGTCACGGGAGTGAAAATGCGAATGGCTCATTAAATCAGT20.13315579227696406No Hit
TGTCACGGGACGCACGGCCGGTACAGTGAAACTGCGAATG20.13315579227696406No Hit
TGTCACGGGAGTGACGAAAAATAACAATACAGGACTCTTT20.13315579227696406No Hit
TGTCACGGGCCTTGGTCAAGTTGGACTTCTCCTGTAATAA20.13315579227696406No Hit
TGTCACGGGAGAAGAAATATTTTTGAAAGGTTATGCATAC20.13315579227696406No Hit
TGTCACGGGATGTGACCAAGTAATTAACTTATAACAGTGT20.13315579227696406No Hit
TGTCACGGGAGAGTCGGTGGGAAAAGGTGGTGCAGCGCCT20.13315579227696406No Hit
TGTCACGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT20.13315579227696406No Hit
TGTCACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13315579227696406No Hit
TGTCACGGGCCCCTGGGGTCAACACAAGCTTGTTTCAATT20.13315579227696406No Hit
TGTCACGGGTACCTGGTTGATACTGCCAGTAGCATATGCT20.13315579227696406No Hit
TGTCACGGGAGCCCACTCCCTGTGTTTCCTTTCTCTCTGG20.13315579227696406No Hit
TGTCACGGGTCGAATTTTTTTTTTTTTTTTTTTTTTTTTT20.13315579227696406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG1500.034.02
TGTCACG1500.034.01
TCACGGG1500.034.03
ACGGGAT251.22016616E-434.05
CACGGGG650.034.04
CACGGGC251.22016616E-434.04
CACGGGA506.002665E-1134.04
ACGGGGG200.00199550533.9999965
ACGGGGA200.00199550533.9999965
Produced by FastQC (version 0.11.5)