##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525883_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1502 Sequences flagged as poor quality 0 Sequence length 40 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 18.542609853528628 27.0 4.0 33.0 4.0 34.0 2 11.292276964047936 4.0 4.0 18.0 4.0 29.0 3 11.920772303595207 4.0 4.0 20.0 4.0 30.0 4 11.559920106524634 4.0 4.0 19.0 4.0 29.0 5 11.85219707057257 4.0 4.0 20.0 4.0 33.0 6 11.544607190412783 4.0 4.0 20.0 4.0 30.0 7 12.004660452729693 4.0 4.0 23.0 4.0 31.0 8 12.179094540612518 4.0 4.0 23.0 4.0 31.0 9 11.888814913448735 4.0 4.0 23.0 4.0 31.0 10 11.163781624500666 4.0 4.0 20.0 4.0 30.0 11 11.55659121171771 4.0 4.0 20.0 4.0 33.0 12 11.301597869507324 4.0 4.0 21.0 4.0 32.0 13 10.586551264980027 4.0 4.0 17.0 4.0 30.0 14 10.790279627163782 4.0 4.0 17.0 4.0 32.0 15 10.22237017310253 4.0 4.0 15.0 4.0 31.0 16 8.46271637816245 4.0 4.0 4.0 4.0 26.0 17 8.242343541944075 4.0 4.0 4.0 4.0 25.0 18 7.923435419440746 4.0 4.0 4.0 4.0 23.0 19 7.860186418109188 4.0 4.0 4.0 4.0 24.0 20 7.900798934753662 4.0 4.0 4.0 4.0 23.0 21 7.322902796271638 4.0 4.0 4.0 4.0 21.0 22 7.238348868175765 4.0 4.0 4.0 4.0 20.0 23 7.386151797603196 4.0 4.0 4.0 4.0 21.0 24 7.207057256990679 4.0 4.0 4.0 4.0 19.0 25 7.440745672436751 4.0 4.0 4.0 4.0 22.0 26 7.741011984021305 4.0 4.0 4.0 4.0 23.0 27 7.701065246338215 4.0 4.0 4.0 4.0 24.0 28 8.000665778961384 4.0 4.0 4.0 4.0 25.0 29 7.343541944074567 4.0 4.0 4.0 4.0 21.0 30 7.673102529960053 4.0 4.0 4.0 4.0 23.0 31 8.151797603195739 4.0 4.0 4.0 4.0 27.0 32 7.994673768308921 4.0 4.0 4.0 4.0 27.0 33 8.047270306258323 4.0 4.0 4.0 4.0 27.0 34 8.064580559254328 4.0 4.0 4.0 4.0 27.0 35 7.839547270306259 4.0 4.0 4.0 4.0 26.0 36 7.813581890812251 4.0 4.0 4.0 4.0 28.0 37 7.79560585885486 4.0 4.0 4.0 4.0 26.0 38 7.720372836218376 4.0 4.0 4.0 4.0 27.0 39 7.567243675099867 4.0 4.0 4.0 4.0 26.0 40 7.634487350199734 4.0 4.0 4.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -12.267763157894734 2 2 -5.850219298245614 2 3 -6.36732456140351 2 4 -6.025 2 5 -6.172149122807017 2 6 -5.678728070175438 2 7 -6.822807017543861 2 8 -6.944736842105264 2 9 -6.697149122807016 2 10 -6.181140350877195 2 11 -6.536842105263158 2 12 -5.756140350877194 2 13 -5.30065789473684 2 14 -5.888377192982455 2 15 -5.574342105263158 2 16 -4.427412280701754 2 17 -4.319517543859648 2 18 -3.58530701754386 2 19 -3.7978070175438594 2 20 -3.97280701754386 2 21 -2.9557017543859647 2 22 -2.547587719298246 2 23 -2.7949561403508785 2 24 -2.7203947368421053 2 25 -2.432675438596492 2 26 -3.025438596491229 2 27 -2.7377192982456133 2 28 -3.1826754385964913 2 29 -2.720175438596492 2 30 -3.0625 2 31 -3.2644736842105253 2 32 -3.2949561403508785 2 33 -3.1802631578947373 2 34 -2.9857456140350873 2 35 -3.2256578947368437 2 36 -2.7427631578947373 2 37 -3.103289473684211 2 38 -2.8484649122807015 2 39 -2.895833333333334 2 40 -3.0046052631578952 5 1 -12.267763157894734 5 2 -5.850219298245614 5 3 -6.36732456140351 5 4 -6.025 5 5 -6.172149122807017 5 6 -5.678728070175438 5 7 -6.822807017543861 5 8 -6.944736842105264 5 9 -6.697149122807016 5 10 -6.181140350877195 5 11 -6.536842105263158 5 12 -5.756140350877194 5 13 -5.30065789473684 5 14 -5.888377192982455 5 15 -5.574342105263158 5 16 -4.427412280701754 5 17 -4.319517543859648 5 18 -3.58530701754386 5 19 -3.7978070175438594 5 20 -3.97280701754386 5 21 -2.9557017543859647 5 22 -2.547587719298246 5 23 -2.7949561403508785 5 24 -2.7203947368421053 5 25 -2.432675438596492 5 26 -3.025438596491229 5 27 -2.7377192982456133 5 28 -3.1826754385964913 5 29 -2.720175438596492 5 30 -3.0625 5 31 -3.2644736842105253 5 32 -3.2949561403508785 5 33 -3.1802631578947373 5 34 -2.9857456140350873 5 35 -3.2256578947368437 5 36 -2.7427631578947373 5 37 -3.103289473684211 5 38 -2.8484649122807015 5 39 -2.895833333333334 5 40 -3.0046052631578952 6 1 16.732236842105266 6 2 15.149780701754386 6 3 11.966008771929822 6 4 15.308333333333332 6 5 9.49451754385965 6 6 2.3212719298245617 6 7 1.1771929824561393 6 8 2.7219298245614016 6 9 2.9695175438596504 6 10 1.4855263157894711 6 11 -5.536842105263158 6 12 -1.4228070175438603 6 13 -1.3006578947368403 6 14 -4.221710526315788 6 15 2.758991228070176 6 16 3.2392543859649123 6 17 4.347149122807018 6 18 1.0813596491228061 6 19 1.2021929824561406 6 20 3.360526315789474 6 21 0.3776315789473683 6 22 0.11907894736842106 6 23 5.5383771929824555 6 24 1.9462719298245608 6 25 1.2339912280701748 6 26 1.641228070175437 6 27 4.928947368421053 6 28 1.4839912280701748 6 29 4.946491228070174 6 30 2.270833333333334 6 31 1.0688596491228086 6 32 6.0383771929824555 6 33 2.1530701754385966 6 34 6.014254385964913 6 35 -3.2256578947368437 6 36 -2.7427631578947373 6 37 -3.103289473684211 6 38 -2.8484649122807015 6 39 -2.895833333333334 6 40 -3.0046052631578952 7 1 -3.601096491228068 7 2 -1.183552631578948 7 3 1.9660087719298236 7 4 -5.025 7 5 2.494517543859649 7 6 -5.678728070175438 7 7 -6.822807017543861 7 8 -6.944736842105264 7 9 -6.697149122807016 7 10 -6.181140350877195 7 11 -6.536842105263158 7 12 -5.756140350877194 7 13 -5.30065789473684 7 14 -5.888377192982455 7 15 -5.574342105263158 7 16 -4.427412280701754 7 17 -4.319517543859648 7 18 -3.58530701754386 7 19 -3.7978070175438594 7 20 -3.97280701754386 7 21 -2.9557017543859647 7 22 -2.547587719298246 7 23 -2.7949561403508785 7 24 -2.7203947368421053 7 25 -2.432675438596492 7 26 -3.025438596491229 7 27 -2.7377192982456133 7 28 -3.1826754385964913 7 29 -2.720175438596492 7 30 -3.0625 7 31 -3.2644736842105253 7 32 -3.2949561403508785 7 33 -3.1802631578947373 7 34 -2.9857456140350873 7 35 -3.2256578947368437 7 36 -2.7427631578947373 7 37 -3.103289473684211 7 38 -2.8484649122807015 7 39 -2.895833333333334 7 40 -3.0046052631578952 8 1 -12.267763157894734 8 2 -5.850219298245614 8 3 -6.36732456140351 8 4 -6.025 8 5 -6.172149122807017 8 6 -5.678728070175438 8 7 -6.822807017543861 8 8 -6.944736842105264 8 9 -6.697149122807016 8 10 -6.181140350877195 8 11 -6.536842105263158 8 12 -5.756140350877194 8 13 -5.30065789473684 8 14 -5.888377192982455 8 15 -5.574342105263158 8 16 -4.427412280701754 8 17 -4.319517543859648 8 18 -3.58530701754386 8 19 -3.7978070175438594 8 20 -3.97280701754386 8 21 -2.9557017543859647 8 22 -2.547587719298246 8 23 -2.7949561403508785 8 24 -2.7203947368421053 8 25 -2.432675438596492 8 26 -3.025438596491229 8 27 -2.7377192982456133 8 28 -3.1826754385964913 8 29 -2.720175438596492 8 30 -3.0625 8 31 -3.2644736842105253 8 32 -3.2949561403508785 8 33 -3.1802631578947373 8 34 -2.9857456140350873 8 35 -3.2256578947368437 8 36 -2.7427631578947373 8 37 -3.103289473684211 8 38 -2.8484649122807015 8 39 -2.895833333333334 8 40 -3.0046052631578952 9 1 -12.267763157894734 9 2 -5.850219298245614 9 3 -6.36732456140351 9 4 -6.025 9 5 -6.172149122807017 9 6 -5.678728070175438 9 7 -6.822807017543861 9 8 -6.944736842105264 9 9 -6.697149122807016 9 10 -6.181140350877195 9 11 -6.536842105263158 9 12 -5.756140350877194 9 13 -5.30065789473684 9 14 -5.888377192982455 9 15 -5.574342105263158 9 16 -4.427412280701754 9 17 -4.319517543859648 9 18 -3.58530701754386 9 19 -3.7978070175438594 9 20 -3.97280701754386 9 21 -2.9557017543859647 9 22 -2.547587719298246 9 23 -2.7949561403508785 9 24 -2.7203947368421053 9 25 -2.432675438596492 9 26 -3.025438596491229 9 27 -2.7377192982456133 9 28 -3.1826754385964913 9 29 -2.720175438596492 9 30 -3.0625 9 31 -3.2644736842105253 9 32 -3.2949561403508785 9 33 -3.1802631578947373 9 34 -2.9857456140350873 9 35 -3.2256578947368437 9 36 -2.7427631578947373 9 37 -3.103289473684211 9 38 -2.8484649122807015 9 39 -2.895833333333334 9 40 -3.0046052631578952 11 1 19.732236842105266 11 2 23.149780701754388 11 3 24.63267543859649 11 4 24.975 11 5 15.827850877192983 11 6 27.32127192982456 11 7 26.17719298245614 11 8 25.055263157894736 11 9 25.302850877192984 11 10 21.818859649122807 11 11 9.463157894736842 11 12 2.2438596491228058 11 13 18.69934210526316 11 14 13.111622807017545 11 15 23.425657894736844 11 16 14.572587719298246 11 17 19.680482456140354 11 18 15.41469298245614 11 19 2.2021929824561406 11 20 20.02719298245614 11 21 19.044298245614037 11 22 20.452412280701754 11 23 17.205043859649123 11 24 15.279605263157894 11 25 17.56732456140351 11 26 17.974561403508773 11 27 27.262280701754385 11 28 18.81732456140351 11 29 21.279824561403508 11 30 15.9375 11 31 11.735526315789475 11 32 14.705043859649122 11 33 11.819736842105263 11 34 13.014254385964913 11 35 24.774342105263155 11 36 7.257236842105263 11 37 17.89671052631579 11 38 23.1515350877193 11 39 21.104166666666664 11 40 24.995394736842105 12 1 18.93223684210527 12 2 19.949780701754385 12 3 13.832675438596489 12 4 17.174999999999997 12 5 19.627850877192984 12 6 22.32127192982456 12 7 24.17719298245614 12 8 22.25526315789474 12 9 22.902850877192986 12 10 23.01885964912281 12 11 19.86315789473684 12 12 22.243859649122804 12 13 13.69934210526316 12 14 21.511622807017545 12 15 20.825657894736842 12 16 2.9725877192982466 12 17 1.880482456140351 12 18 6.81469298245614 12 19 3.0021929824561413 12 20 5.02719298245614 12 21 5.644298245614035 12 22 8.652412280701753 12 23 10.805043859649123 12 24 6.479605263157894 12 25 8.567324561403508 12 26 9.574561403508772 12 27 12.462280701754386 12 28 10.81732456140351 12 29 8.879824561403508 12 30 9.9375 12 31 14.135526315789473 12 32 10.90504385964912 12 33 14.019736842105262 12 34 11.414254385964911 12 35 13.774342105263155 12 36 6.657236842105263 12 37 14.89671052631579 12 38 10.551535087719298 12 39 11.104166666666666 12 40 11.595394736842106 15 1 17.232236842105266 15 2 13.899780701754386 15 3 14.38267543859649 15 4 13.225 15 5 18.577850877192983 15 6 23.82127192982456 15 7 24.42719298245614 15 8 22.055263157894736 15 9 17.052850877192984 15 10 18.068859649122807 15 11 15.963157894736842 15 12 16.743859649122804 15 13 18.44934210526316 15 14 11.111622807017545 15 15 15.175657894736842 15 16 9.822587719298246 15 17 4.180482456140352 15 18 5.91469298245614 15 19 6.952192982456141 15 20 9.777192982456139 15 21 11.294298245614035 15 22 11.452412280701754 15 23 7.2050438596491215 15 24 6.279605263157895 15 25 3.317324561403508 15 26 11.22456140350877 15 27 11.012280701754387 15 28 10.06732456140351 15 29 6.779824561403508 15 30 13.6875 15 31 13.485526315789475 15 32 8.705043859649122 15 33 13.319736842105263 15 34 9.764254385964913 15 35 12.274342105263155 15 36 9.507236842105263 15 37 14.39671052631579 15 38 14.9015350877193 15 39 16.104166666666664 15 40 17.745394736842105 16 1 13.732236842105266 16 2 7.149780701754386 16 3 9.63267543859649 16 4 -0.025000000000000355 16 5 4.827850877192983 16 6 -0.6787280701754383 16 7 16.17719298245614 16 8 16.055263157894736 16 9 18.302850877192984 16 10 18.818859649122807 16 11 23.46315789473684 16 12 17.243859649122804 16 13 2.6993421052631597 16 14 22.111622807017547 16 15 20.425657894736844 16 16 22.572587719298248 16 17 6.680482456140352 16 18 14.41469298245614 16 19 23.20219298245614 16 20 23.02719298245614 16 21 25.044298245614037 16 22 14.452412280701754 16 23 22.205043859649123 16 24 22.279605263157894 16 25 17.56732456140351 16 26 18.974561403508773 16 27 15.262280701754387 16 28 23.81732456140351 16 29 12.279824561403508 16 30 8.9375 16 31 9.735526315789475 16 32 10.705043859649122 16 33 1.8197368421052627 16 34 11.014254385964913 16 35 22.774342105263155 16 36 -2.7427631578947373 16 37 -3.103289473684211 16 38 -2.8484649122807015 16 39 -2.895833333333334 16 40 -3.0046052631578952 17 1 6.065570175438598 17 2 -4.183552631578947 17 3 -3.7006578947368434 17 4 -2.6916666666666673 17 5 3.494517543859649 17 6 2.6546052631578956 17 7 -0.4894736842105267 17 8 -0.6114035087719305 17 9 0.9695175438596504 17 10 -0.847807017543861 17 11 0.7964912280701757 17 12 2.910526315789472 17 13 3.6993421052631597 17 14 3.111622807017545 17 15 5.092324561403508 17 16 -4.427412280701754 17 17 -4.319517543859648 17 18 -3.58530701754386 17 19 -3.7978070175438594 17 20 -3.97280701754386 17 21 -2.9557017543859647 17 22 -2.547587719298246 17 23 -2.7949561403508785 17 24 -2.7203947368421053 17 25 -2.432675438596492 17 26 -3.025438596491229 17 27 -2.7377192982456133 17 28 -3.1826754385964913 17 29 -2.720175438596492 17 30 -3.0625 17 31 -3.2644736842105253 17 32 -3.2949561403508785 17 33 -3.1802631578947373 17 34 -2.9857456140350873 17 35 -3.2256578947368437 17 36 -2.7427631578947373 17 37 -3.103289473684211 17 38 -2.8484649122807015 17 39 -2.895833333333334 17 40 -3.0046052631578952 18 1 4.398903508771934 18 2 -0.18355263157894797 18 3 2.9660087719298236 18 4 0.30833333333333357 18 5 0.49451754385964897 18 6 -2.0120614035087714 18 7 5.177192982456139 18 8 6.388596491228068 18 9 3.3028508771929843 18 10 3.485526315789471 18 11 13.463157894736842 18 12 14.243859649122806 18 13 10.366008771929828 18 14 14.778289473684213 18 15 11.425657894736842 18 16 8.239254385964914 18 17 9.34714912280702 18 18 1.748026315789474 18 19 12.20219298245614 18 20 13.027192982456139 18 21 2.7109649122807014 18 22 3.785745614035088 18 23 2.8717105263157876 18 24 3.9462719298245608 18 25 6.567324561403508 18 26 0.9745614035087709 18 27 4.595614035087721 18 28 4.150657894736843 18 29 2.279824561403508 18 30 6.604166666666666 18 31 -3.2644736842105253 18 32 -3.2949561403508785 18 33 -3.1802631578947373 18 34 -2.9857456140350873 18 35 -3.2256578947368437 18 36 -2.7427631578947373 18 37 -3.103289473684211 18 38 -2.8484649122807015 18 39 -2.895833333333334 18 40 -3.0046052631578952 19 1 15.732236842105266 19 2 0.649780701754386 19 3 10.63267543859649 19 4 7.725 19 5 8.827850877192983 19 6 10.821271929824562 19 7 16.92719298245614 19 8 16.805263157894736 19 9 16.052850877192984 19 10 18.068859649122807 19 11 22.46315789473684 19 12 15.743859649122806 19 13 19.69934210526316 19 14 12.111622807017545 19 15 10.675657894736842 19 16 14.822587719298246 19 17 15.680482456140352 19 18 3.66469298245614 19 19 14.20219298245614 19 20 14.277192982456139 19 21 9.294298245614035 19 22 6.202412280701754 19 23 4.7050438596491215 19 24 12.529605263157894 19 25 16.31732456140351 19 26 10.22456140350877 19 27 8.262280701754387 19 28 8.81732456140351 19 29 8.279824561403508 19 30 10.4375 19 31 5.485526315789475 19 32 7.9550438596491215 19 33 2.8197368421052627 19 34 5.764254385964913 19 35 4.774342105263156 19 36 8.507236842105263 19 37 10.89671052631579 19 38 4.4015350877192985 19 39 4.354166666666666 19 40 4.245394736842105 20 1 -12.267763157894734 20 2 -5.850219298245614 20 3 -6.36732456140351 20 4 -6.025 20 5 -6.172149122807017 20 6 -5.678728070175438 20 7 -6.822807017543861 20 8 -6.944736842105264 20 9 -6.697149122807016 20 10 -6.181140350877195 20 11 -6.536842105263158 20 12 -5.756140350877194 20 13 -5.30065789473684 20 14 -5.888377192982455 20 15 -5.574342105263158 20 16 -4.427412280701754 20 17 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120 4 1.9749999999999996 120 5 5.827850877192983 120 6 10.321271929824562 120 7 12.17719298245614 120 8 11.055263157894736 120 9 7.302850877192984 120 10 1.818859649122805 120 11 18.46315789473684 120 12 16.243859649122804 120 13 11.69934210526316 120 14 19.111622807017547 120 15 17.425657894736844 120 16 20.572587719298248 120 17 21.680482456140354 120 18 21.41469298245614 120 19 25.20219298245614 120 20 -3.97280701754386 120 21 -2.9557017543859647 120 22 -2.547587719298246 120 23 -2.7949561403508785 120 24 -2.7203947368421053 120 25 -2.432675438596492 120 26 -3.025438596491229 120 27 -2.7377192982456133 120 28 -3.1826754385964913 120 29 -2.720175438596492 120 30 -3.0625 120 31 -3.2644736842105253 120 32 -3.2949561403508785 120 33 -3.1802631578947373 120 34 -2.9857456140350873 120 35 -3.2256578947368437 120 36 -2.7427631578947373 120 37 -3.103289473684211 120 38 -2.8484649122807015 120 39 -2.895833333333334 120 40 -3.0046052631578952 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 860.0 5 22.0 6 38.0 7 19.0 8 23.0 9 43.0 10 56.0 11 46.0 12 31.0 13 27.0 14 27.0 15 13.0 16 10.0 17 6.0 18 7.0 19 16.0 20 31.0 21 38.0 22 38.0 23 38.0 24 27.0 25 38.0 26 15.0 27 14.0 28 10.0 29 4.0 30 2.0 31 2.0 32 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.06790945406125 0.5326231691078562 0.06657789613848203 0.3328894806924101 8 98.8 0.33333333333333337 0.6 0.26666666666666666 9 98.86666666666667 0.33333333333333337 0.5333333333333333 0.26666666666666666 10 49.533954727030626 27.563249001331556 12.916111850865514 9.986684420772303 11 30.492676431424766 21.904127829560586 27.430093209054597 20.17310252996005 12 28.828229027962713 21.770972037283624 26.2982689747004 23.10252996005326 13 22.969374167776298 21.70439414114514 28.56191744340879 26.764314247669773 14 18.575233022636482 30.22636484687084 31.89081225033289 19.307589880159785 15 29.294274300932088 25.099866844207725 27.96271637816245 17.643142476697736 16 32.15712383488682 27.430093209054597 24.167776298268976 16.245006657789617 17 25.449700199866754 23.850766155896068 33.44437041972019 17.25516322451699 18 22.63648468708389 24.30093209054594 30.09320905459387 22.969374167776298 19 25.499334221038616 26.564580559254324 27.82956058588549 20.10652463382157 20 26.165113182423433 26.098535286284953 30.426098535286282 17.310252996005325 21 25.166444740346204 22.769640479360852 30.02663115845539 22.03728362183755 22 20.77230359520639 20.70572569906791 35.41944074567244 23.10252996005326 23 18.242343541944077 27.563249001331556 32.822902796271634 21.371504660452732 24 21.052631578947366 24.716855429713526 30.246502331778814 23.984010659560294 25 24.816544362908605 30.95396931287525 26.21747831887925 18.01200800533689 26 27.67379679144385 28.00802139037433 22.86096256684492 21.4572192513369 27 19.238476953907817 30.72812291249165 25.517702070808284 24.51569806279225 28 20.77230359520639 30.62583222370173 26.830892143808256 21.770972037283624 29 27.00534759358289 29.27807486631016 24.197860962566846 19.518716577540108 30 24.167776298268976 27.563249001331556 29.094540612516646 19.174434087882823 31 24.766977363515313 30.02663115845539 27.030625832223702 18.17576564580559 32 29.47860962566845 26.203208556149733 25.13368983957219 19.184491978609625 33 24.36750998668442 28.29560585885486 25.832223701731028 21.50466045272969 34 20.505992010652463 25.96537949400799 34.953395472703065 18.575233022636482 35 19.04127829560586 28.29560585885486 34.88681757656458 17.7762982689747 36 21.977287909151634 27.85571142284569 29.258517034068138 20.908483633934534 37 21.904127829560586 27.496671105193077 31.69107856191744 18.908122503328894 38 24.96671105193076 26.764314247669773 29.094540612516646 19.174434087882823 39 23.56857523302264 25.5659121171771 28.1624500665779 22.70306258322237 40 22.63648468708389 24.500665778961384 32.9560585885486 19.906790945406126 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 2.0 16 4.5 17 7.0 18 7.0 19 9.5 20 12.0 21 15.5 22 19.0 23 19.0 24 14.0 25 9.0 26 8.0 27 7.0 28 7.0 29 16.0 30 25.0 31 21.5 32 18.0 33 18.0 34 29.0 35 40.0 36 43.5 37 47.0 38 47.0 39 63.0 40 79.0 41 94.0 42 109.0 43 109.0 44 119.5 45 130.0 46 132.0 47 134.0 48 134.0 49 135.5 50 137.0 51 144.0 52 151.0 53 151.0 54 155.5 55 160.0 56 147.5 57 135.0 58 135.0 59 120.5 60 106.0 61 94.5 62 83.0 63 83.0 64 62.0 65 41.0 66 34.0 67 27.0 68 27.0 69 22.5 70 18.0 71 11.0 72 4.0 73 4.0 74 2.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.5 82 1.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.13315579227696406 9 0.13315579227696406 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.06657789613848203 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.06657789613848203 25 0.1997336884154461 26 0.3994673768308922 27 0.3328894806924101 28 0.0 29 0.3994673768308922 30 0.0 31 0.0 32 0.3994673768308922 33 0.0 34 0.0 35 0.0 36 0.3328894806924101 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1502.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.14513981358189 #Duplication Level Percentage of deduplicated Percentage of total 1 94.66764061358656 86.28495339547271 2 4.090577063550037 7.456724367509987 3 0.5843681519357196 1.5978695073235687 4 0.2191380569758948 0.7989347536617843 5 0.07304601899196494 0.3328894806924101 6 0.07304601899196494 0.3994673768308922 7 0.07304601899196494 0.4660452729693742 8 0.07304601899196494 0.5326231691078562 9 0.0 0.0 >10 0.1460920379839299 2.130492676431425 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 20 1.3315579227696404 No Hit TGTCACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 12 0.7989347536617843 No Hit TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 8 0.5326231691078562 No Hit TGTCACGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT 7 0.4660452729693742 No Hit TGTCACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 6 0.3994673768308922 No Hit TGTCACGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 5 0.3328894806924101 No Hit TGTCACGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 4 0.2663115845539281 No Hit TGTCACGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT 4 0.2663115845539281 No Hit TGTCACGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCT 4 0.2663115845539281 No Hit TGTCACGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGA 3 0.1997336884154461 No Hit TGTCACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA 3 0.1997336884154461 No Hit TGTCACGGGGGGGAGGTAGTGACGAAAAATAACAATACAG 3 0.1997336884154461 No Hit TGTCACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.1997336884154461 No Hit TGTCACGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTA 3 0.1997336884154461 No Hit TGTCACGGGGTTGTTGTTTTTGTTTTTAACTTGCAAGCCA 3 0.1997336884154461 No Hit TGTCACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT 3 0.1997336884154461 No Hit TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 3 0.1997336884154461 No Hit TGTCACGGGTCACAGATCCCCTTCTGAAGGAGATTGGGCG 2 0.13315579227696406 No Hit TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC 2 0.13315579227696406 No Hit TGTCACGGGATAGTTGTTCTTTCATCACGATCTTATGGCG 2 0.13315579227696406 No Hit TGTCACGGGGAGGGGTTGGAGAACTGGCTTCGCGGTGTGT 2 0.13315579227696406 No Hit TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 2 0.13315579227696406 No Hit TGTCACGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGC 2 0.13315579227696406 No Hit TGTCACGGGCGACCTACCTGGAATAACGAAAGTGCCCGTG 2 0.13315579227696406 No Hit TGTCACGGGACTAATCCTAGCCCTAGCCCTACACAAATAT 2 0.13315579227696406 No Hit TGTCACGGGGCTGCGCTCCAATTGCGACTCTAGCCTTTTC 2 0.13315579227696406 No Hit TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAAAC 2 0.13315579227696406 No Hit TGTCACGGGAGGGTTGTTCTGTGTAGCTTTGGCTCTCCTA 2 0.13315579227696406 No Hit TGTCACGGGGTTTTTTAATGTTTCATGACTCCCACTTTGA 2 0.13315579227696406 No Hit TGTCACGGGATGTTTAAGTTTTCTGGGTTACTGGATTTCA 2 0.13315579227696406 No Hit TGTCACGGGGCCAAGGCTGAGGAAGATCAAGCATGCCCGG 2 0.13315579227696406 No Hit TGTCACGGGACTTTAAATCCTTTAACGAGGATCCATTGGA 2 0.13315579227696406 No Hit TGTCACGGGGGAGGGGTGTATATTTTGATAAAATACTTGA 2 0.13315579227696406 No Hit TGTCACGGGCTACAGCTTTACCACCACTGCTGAGAGGGAG 2 0.13315579227696406 No Hit TGTCACGGGGAAGCTAGCTTGCATTCTCCCCTCATTCATC 2 0.13315579227696406 No Hit TGTCACGGGAGCATTTTTTACTTTCAATGCCTTAGCTAGC 2 0.13315579227696406 No Hit TGTCACGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT 2 0.13315579227696406 No Hit TGTCACGGGGGTCGGGCGCCGGCGGCTTTGGTGACTCTAG 2 0.13315579227696406 No Hit TGTCACGGGGGGCACCATGGTTCTGACCAATTTGACAGCT 2 0.13315579227696406 No Hit TGTCACGGGTCATAGATCCTCTTCTGAGATGAGTTTTTGT 2 0.13315579227696406 No Hit TGTCACGGGGGCTATATAGGAATAGTATGAGCAATAATGT 2 0.13315579227696406 No Hit TGTCACGGGAGCTGGTGCCATGGATGTTCCTCTGACTTAG 2 0.13315579227696406 No Hit TGTCACGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT 2 0.13315579227696406 No Hit TGTCACGGGGTTTGTTTTGTGTTTTGAGGTAGGGTCTCTA 2 0.13315579227696406 No Hit TGTCACGGGATCCCAAATACTGGTTAACTCCCCCGGCCTT 2 0.13315579227696406 No Hit TGTCACGGGGTGTTACTTGACCTTTTTCCCTTTCTGCTTT 2 0.13315579227696406 No Hit TGTCACGGGAGGAAGTATACTGTGTGCTTTTATATAACCA 2 0.13315579227696406 No Hit TGTCACGGGTGTGCGCTCCGTGTTAGTGTAGCCCTCCACG 2 0.13315579227696406 No Hit TGTCACGGGGACTTTGAGAAACTCTGCTCCATTTCTCTCT 2 0.13315579227696406 No Hit TGTCACGGGGTTCTATCAATGGGAGCAGTGTTTGCTATCA 2 0.13315579227696406 No Hit TGTCACGGGGGGTGCTGGAATTCTAGGCCTGCATGGCCTC 2 0.13315579227696406 No Hit TGTCACGGGGTTATAAATTAACTTATTTCTAGATTGTTTT 2 0.13315579227696406 No Hit TGTCACGGGGTGCTCTCCTCGCCTGATCATGACCATGCAC 2 0.13315579227696406 No Hit TGTCACGGGCCCTGGACCTTTTGGATTTGAGATTACTAGG 2 0.13315579227696406 No Hit TGTCACGGGCATTACCTTTATGTTACTTTATGCTGCTGGC 2 0.13315579227696406 No Hit TGTCACGGGCTCCTGTGTAGCCCTGTGGTGCAATGCGACG 2 0.13315579227696406 No Hit TGTCACGGGGGAATTGTTTTATCCAACTCATCCCTTGACA 2 0.13315579227696406 No Hit TGTCACGGGTGACTTAGGAAGTTTCCAGGTAGACTTGGAG 2 0.13315579227696406 No Hit TGTCACGGGGTTTGAAAATGTCAGGTCTTCTGCCTCTTGT 2 0.13315579227696406 No Hit TGTCACGGGACCCGGGGAGGTAGTGACGAAAAATAACAAT 2 0.13315579227696406 No Hit TGTCACGGGAGTGAAAATGCGAATGGCTCATTAAATCAGT 2 0.13315579227696406 No Hit TGTCACGGGACGCACGGCCGGTACAGTGAAACTGCGAATG 2 0.13315579227696406 No Hit TGTCACGGGAGTGACGAAAAATAACAATACAGGACTCTTT 2 0.13315579227696406 No Hit TGTCACGGGCCTTGGTCAAGTTGGACTTCTCCTGTAATAA 2 0.13315579227696406 No Hit TGTCACGGGAGAAGAAATATTTTTGAAAGGTTATGCATAC 2 0.13315579227696406 No Hit TGTCACGGGATGTGACCAAGTAATTAACTTATAACAGTGT 2 0.13315579227696406 No Hit TGTCACGGGAGAGTCGGTGGGAAAAGGTGGTGCAGCGCCT 2 0.13315579227696406 No Hit TGTCACGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT 2 0.13315579227696406 No Hit TGTCACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.13315579227696406 No Hit TGTCACGGGCCCCTGGGGTCAACACAAGCTTGTTTCAATT 2 0.13315579227696406 No Hit TGTCACGGGTACCTGGTTGATACTGCCAGTAGCATATGCT 2 0.13315579227696406 No Hit TGTCACGGGAGCCCACTCCCTGTGTTTCCTTTCTCTCTGG 2 0.13315579227696406 No Hit TGTCACGGGTCGAATTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.13315579227696406 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.06657789613848203 0.0 0.0 0.0 0.0 13 0.06657789613848203 0.0 0.0 0.0 0.0 14 0.06657789613848203 0.0 0.0 0.0 0.0 15 0.06657789613848203 0.0 0.0 0.0 0.0 16 0.06657789613848203 0.0 0.0 0.0 0.0 17 0.06657789613848203 0.0 0.0 0.0 0.0 18 0.06657789613848203 0.0 0.0 0.0 0.0 19 0.06657789613848203 0.0 0.0 0.0 0.0 20 0.06657789613848203 0.0 0.0 0.0 0.0 21 0.06657789613848203 0.0 0.0 0.0 0.0 22 0.06657789613848203 0.0 0.0 0.0 0.0 23 0.06657789613848203 0.0 0.0 0.0 0.0 24 0.06657789613848203 0.0 0.0 0.0 0.0 25 0.06657789613848203 0.0 0.0 0.0 0.0 26 0.06657789613848203 0.0 0.0 0.0 0.0 27 0.06657789613848203 0.0 0.0 0.0 0.0 28 0.06657789613848203 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCACGG 150 0.0 34.0 2 TGTCACG 150 0.0 34.0 1 TCACGGG 150 0.0 34.0 3 ACGGGAT 25 1.22016616E-4 34.0 5 CACGGGG 65 0.0 34.0 4 CACGGGC 25 1.22016616E-4 34.0 4 CACGGGA 50 6.002665E-11 34.0 4 ACGGGGG 20 0.001995505 33.999996 5 ACGGGGA 20 0.001995505 33.999996 5 >>END_MODULE