FastQCFastQC Report
Fri 17 Jun 2016
SRR1525882_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525882_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70357
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT8831.2550279289907187No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG7551.0730986255809656No Hit
CCATCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC6100.8670068365621046No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2800.39797035120883495No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2770.39370638316016887No Hit
CCATCTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT2690.3823358016970593No Hit
CCATCTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC2410.3425387665761758No Hit
CCATCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC2180.3098483448697358No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG2100.29847776340662624No Hit
CCATCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1910.27147263243174097No Hit
CCATCTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1360.19329988487286268No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1330.1890359168241966No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1190.16913739926375485No Hit
CCATCTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC1140.16203078584931138No Hit
CCATCTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1120.15918814048353397No Hit
CCATCTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA1050.14923888170331312No Hit
CCATCTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC1030.1463962363375357No Hit
CCATCTGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC1000.1421322682888696No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG940.13360433219153744No Hit
CCATCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC910.12934036414287137No Hit
CCATCTGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG800.11370581463109569No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG800.11370581463109569No Hit
CCATCTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG750.10659920121665221No Hit
CCATCTGGGGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA750.10659920121665221No Hit
CCATCTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC740.10517787853376352No Hit
CCATCTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT730.10375655585087482No Hit
CCATCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC730.10375655585087482No Hit
CCATCTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC710.10091391048509743No Hit
CCATCTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT710.10091391048509743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTA150.00821484350.2797740
TTGTCTA150.00821484350.2797737
CGATTGA309.946234E-750.2797740
AGCGCTT150.00821484350.2797737
AAGAGAC204.0581689E-450.2437335
ATGCTCG204.0581689E-450.2437336
TATGCCT150.00823818550.2437335
AATACCT204.0581689E-450.2437335
TAGAGCG150.00826157550.20773734
ATATGCA150.00826157550.20773734
ACTTATC150.00828501650.171846
ATACTAT150.00828501650.171845
TACTATT150.00828501650.171846
TATTGTT150.00830850550.13590643
TTATACT150.00830850550.13590643
ATTATAC150.00830850550.13590642
TATACTA150.00830850550.13590644
TTATTGT150.00830850550.13590642
TGTCAAA150.00835563250.06428549
CAAAACT204.1305448E-450.06428549
Produced by FastQC (version 0.11.5)