##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525882_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70357 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.87869010901545 39.0 38.0 40.0 35.0 40.0 2 37.484173571926036 39.0 38.0 40.0 33.0 40.0 3 36.58464687237944 39.0 38.0 40.0 31.0 40.0 4 37.103088534189915 39.0 38.0 40.0 32.0 40.0 5 36.87434086160581 39.0 38.0 40.0 32.0 40.0 6 36.821680856204786 39.0 38.0 40.0 32.0 40.0 7 37.38188097843854 39.0 38.0 40.0 33.0 40.0 8 37.15969981664937 39.0 38.0 40.0 33.0 40.0 9 37.14068251915232 39.0 38.0 40.0 33.0 40.0 10 36.2542319882883 39.0 36.0 40.0 30.0 40.0 11 35.73196696846085 38.0 35.0 40.0 29.0 40.0 12 34.54931279048282 38.0 34.0 40.0 23.0 40.0 13 33.99884872862686 38.0 33.0 40.0 21.0 40.0 14 34.583836718450186 38.0 34.0 40.0 23.0 40.0 15 33.34425856702247 38.0 32.0 39.0 18.0 40.0 16 32.86667993234504 38.0 30.0 39.0 19.0 40.0 17 31.838637235811646 36.0 28.0 39.0 15.0 40.0 18 31.252768025924926 36.0 27.0 39.0 14.0 40.0 19 32.45736742612675 36.0 29.0 39.0 18.0 40.0 20 33.01056042753386 37.0 30.0 39.0 21.0 40.0 21 33.20873544920904 38.0 31.0 39.0 19.0 40.0 22 32.403371377403815 37.0 29.0 39.0 16.0 40.0 23 33.16407749051267 38.0 31.0 39.0 19.0 40.0 24 32.49608425600864 37.0 30.0 39.0 17.0 40.0 25 32.958298392484046 38.0 31.0 39.0 19.0 40.0 26 33.37137740381199 38.0 32.0 39.0 19.0 40.0 27 33.63213326321475 38.0 33.0 39.0 21.0 40.0 28 33.60013928962292 38.0 33.0 39.0 21.0 40.0 29 33.06407322654462 38.0 32.0 39.0 18.0 40.0 30 32.88170331310317 37.0 31.0 39.0 18.0 40.0 31 33.68853134727177 38.0 33.0 39.0 21.0 40.0 32 33.56949557257984 38.0 33.0 39.0 21.0 40.0 33 33.348181417627245 38.0 33.0 39.0 20.0 40.0 34 33.18471509586821 37.0 33.0 39.0 19.0 40.0 35 33.17947041516835 38.0 33.0 39.0 19.0 40.0 36 32.35810224995381 36.0 31.0 39.0 17.0 40.0 37 32.129809400628226 36.0 31.0 38.0 15.0 40.0 38 31.435734894893187 36.0 30.0 38.0 9.0 40.0 39 30.47807609761644 35.0 28.0 38.0 4.0 40.0 40 28.07343974302486 34.0 20.0 38.0 4.0 40.0 41 26.24799238171042 31.0 16.0 37.0 4.0 39.0 42 27.156473414159215 32.0 20.0 37.0 4.0 39.0 43 27.323137711954747 32.0 21.0 38.0 4.0 39.0 44 26.449123754566 31.0 18.0 37.0 4.0 39.0 45 27.342950950154215 32.0 21.0 38.0 4.0 39.0 46 25.348209844080902 30.0 17.0 36.0 4.0 39.0 47 24.36879059652913 28.0 11.0 36.0 4.0 39.0 48 25.037366573333145 29.0 16.0 36.0 4.0 39.0 49 24.031098540301606 28.0 10.0 35.0 4.0 39.0 50 24.664980030416306 29.0 14.0 36.0 4.0 39.0 51 24.928052645792175 30.0 10.0 36.0 4.0 39.0 52 24.751424875989596 30.0 4.0 36.0 4.0 39.0 53 24.59738192361812 30.0 4.0 36.0 4.0 39.0 54 24.228363915459727 30.0 4.0 36.0 4.0 39.0 55 23.959861847435224 30.0 4.0 36.0 4.0 39.0 56 23.419645522122888 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.686135859967635 1 2 2.2396132976212044 1 3 3.3724744857322904 1 4 2.618900858184851 1 5 2.822873887670589 1 6 3.1636706775080583 1 7 2.4054266538731213 1 8 2.6091394389409004 1 9 2.551646657825117 1 10 3.7267620662388268 1 11 0.7702917595773116 1 12 -7.002154316106303 1 13 -19.313694418218205 1 14 -6.450394827673382 1 15 1.5552730979696214 1 16 -0.22072848566287462 1 17 -4.892596797208363 1 18 -2.6485857562789548 1 19 0.5214214323211408 1 20 0.0827689015767561 1 21 6.2421800650989 1 22 7.565576771396604 1 23 6.213158268743914 1 24 7.226302701592754 1 25 6.5696719161853245 1 26 6.757057563301085 1 27 6.264866971453099 1 28 6.270867006826787 1 29 6.7902773645268795 1 30 7.120693715509361 1 31 5.509648909187938 1 32 5.5675076578708484 1 33 5.666639584385262 1 34 5.734206079135102 1 35 6.07449251168995 1 36 4.973738623902996 1 37 5.6195782119523905 1 38 6.667832151203605 1 39 5.968890828202728 1 40 -9.692122563568189 1 41 2.092241603114676 1 42 0.898585202098527 1 43 -6.376118166137278 1 44 -15.365842483188764 1 45 0.39213653304394214 1 46 -3.7483154591110086 1 47 -9.401720017536029 1 48 -2.76704028935756 1 49 -13.44195470246356 1 50 -2.370097404454185 1 51 5.4443038499581675 1 52 5.546402290740481 1 53 5.587442603687684 1 54 5.960361037353138 1 55 5.920564977866814 1 56 9.37541597400234 2 1 1.8999645493381223 2 2 2.4338341438544333 2 3 3.472577684906696 2 4 2.7615221172147812 2 5 2.8347417927273497 2 6 3.251493174928079 2 7 2.375602092469613 2 8 2.4217297382185095 2 9 2.517074934398906 2 10 3.6257300744947614 2 11 3.171942946367814 2 12 -1.8905960085727642 2 13 0.8447163144959333 2 14 3.9885112610779103 2 15 4.997687958650733 2 16 4.333657479249407 2 17 6.410911974721461 2 18 3.625408051770581 2 19 5.6514523920734625 2 20 4.778228137902868 2 21 6.628660973251634 2 22 7.153708866339844 2 23 6.706037525709856 2 24 7.664383196948794 2 25 6.628701843945901 2 26 5.9910100916808595 2 27 4.929056857934011 2 28 5.712043477415019 2 29 6.251784072473221 2 30 6.874150887851982 2 31 5.055572541798881 2 32 5.552750175930704 2 33 5.302140100381134 2 34 5.805826306173284 2 35 5.46210861076116 2 36 6.461870718846235 2 37 6.500383165512762 2 38 6.558131428809382 2 39 6.605836132640288 2 40 5.015926972035526 2 41 9.710920653682273 2 42 8.447398411592847 2 43 7.804067592376654 2 44 -4.489062297430248 2 45 8.078823839545485 2 46 7.027329535729034 2 47 2.9830065046517937 2 48 6.15153556203563 2 49 -1.8507266322881222 2 50 6.605134793688229 2 51 9.973302817966424 2 52 10.060127780936558 2 53 10.771756329177876 2 54 10.399163926930022 2 55 9.979491706452986 2 56 11.299977377511109 3 1 2.056470788197302 3 2 2.646311862214503 3 3 3.5519003230528696 3 4 3.0399534897638034 3 5 3.202299724991164 3 6 3.4732400554984864 3 7 2.8422687591362745 3 8 2.726364319323679 3 9 2.7349002463418657 3 10 3.777958238487635 3 11 4.059286974888316 3 12 5.202630372888912 3 13 5.550420414317678 3 14 4.911327660364897 3 15 1.2356558730892395 3 16 6.845778691370615 3 17 7.426063489872977 3 18 7.981557784390905 3 19 6.988885547153675 3 20 6.097122968562406 3 21 7.328304467012771 3 22 8.204332752257844 3 23 7.096411857260659 3 24 7.916254854702803 3 25 7.192159954462834 3 26 6.1790671326790765 3 27 6.349555966668415 3 28 5.934503370463151 3 29 6.200803680316355 3 30 6.481459265748597 3 31 5.090510153207077 3 32 5.018703830119655 3 33 5.480036713571867 3 34 4.937555361431752 3 35 5.452482942311963 3 36 6.1670400593096915 3 37 6.9554633794165035 3 38 7.901803443069632 3 39 8.848795134422819 3 40 9.966729111072958 3 41 12.213772703593143 3 42 11.357915345639196 3 43 11.201393795585211 3 44 11.881525937863866 3 45 10.755294427780782 3 46 12.789005115051673 3 47 13.2523469681099 3 48 12.511428610163975 3 49 13.397758216196724 3 50 12.358610729517103 3 51 12.362964137039508 3 52 12.637311381649571 3 53 13.041988058233137 3 54 12.506294051707204 3 55 12.790899906096481 3 56 11.015128892662627 4 1 1.994233025959538 4 2 2.5380950789977206 4 3 3.351725497878043 4 4 2.8111073359176473 4 5 2.8886633613548014 4 6 3.310128167386601 4 7 2.6961149129824307 4 8 2.312378305337667 4 9 2.6852498966915093 4 10 3.584251944781336 4 11 4.315231030832372 4 12 5.400882121140661 4 13 5.740455379352646 4 14 4.821467520504754 4 15 5.846669859103226 4 16 7.173401068992991 4 17 8.23462992343941 4 18 8.474389952223074 4 19 7.012312120580251 4 20 6.80726282870226 4 21 6.941066704775011 4 22 6.772864220789312 4 23 6.160223046071845 4 24 7.271849260297206 4 25 6.74163547893836 4 26 5.730640559252503 4 27 5.489765756878203 4 28 4.905482391442174 4 29 6.195034449547123 4 30 5.955410314699648 4 31 4.785964698661623 4 32 5.05576676718259 4 33 5.478987762522916 4 34 4.751716200592593 4 35 4.7912941311231485 4 36 6.225256842526477 4 37 5.950043798996926 4 38 6.889390855657048 4 39 7.802291637919328 4 40 2.7336871530310027 4 41 10.625136339956782 4 42 9.582390870114718 4 43 10.21922596341738 4 44 10.875756707094634 4 45 9.178371350857702 4 46 12.154214905261462 4 47 11.70566864643158 4 48 12.196218819954183 4 49 11.258247726686239 4 50 12.523121219027594 4 51 11.715761339836714 4 52 10.65986382920202 4 53 11.602827219072296 4 54 11.56451083492399 4 55 11.472892913089485 4 56 10.662156864690598 5 1 1.9162110479375585 5 2 2.222069437972081 5 3 3.345406816559361 5 4 2.8209058707161816 5 5 3.0719966946881314 5 6 3.4147984970569283 5 7 2.7041735210410422 5 8 2.478312371271727 5 9 2.8669348783764974 5 10 3.57839113892053 5 11 3.9181614337627764 5 12 4.956742927001464 5 13 5.6612429251401934 5 14 5.078427227464466 5 15 6.592365829799196 5 16 7.20984795543988 5 17 8.044245308054794 5 18 8.496276399109515 5 19 7.539967798235931 5 20 7.168984440423873 5 21 6.9975685362768445 5 22 8.049787297712395 5 23 6.376065536914336 5 24 6.781189919637871 5 25 6.677441339744224 5 26 6.38512773873968 5 27 5.619252936365381 5 28 5.651819387779167 5 29 6.353184632697307 5 30 6.613926798216127 5 31 4.83596469866162 5 32 5.095327206743029 5 33 5.155361388896541 5 34 5.187338911215303 5 35 5.37023186006088 5 36 6.753187245456878 5 37 6.120831344784474 5 38 7.05028829155448 5 39 7.764379550007238 5 40 9.291837336181182 5 41 11.583469673290118 5 42 10.419387207111054 5 43 11.497064791256207 5 44 11.152954509292439 5 45 10.855294427780784 5 46 11.868225894272449 5 47 11.985247401010337 5 48 12.360779259514622 5 49 12.62070193914045 5 50 12.145190816097191 5 51 10.485907859983232 5 52 10.83211657645477 5 53 9.777918794163874 5 54 10.906726952140108 5 55 10.722068737265314 5 56 8.65712023465397 6 1 2.0672314561008207 6 2 2.396219097836024 6 3 3.443366000232828 6 4 3.0746473673148245 6 5 3.0366225450282727 6 6 3.2086760480773364 6 7 2.7565544734219927 6 8 2.5109654324962207 6 9 2.680540320553362 6 10 3.6117244722538686 6 11 4.316800889545085 6 12 4.970348369178339 6 13 5.861923197249034 6 14 5.3532571594372556 6 15 6.369236578098512 6 16 7.194201696936478 6 17 8.213633063156838 6 18 8.241854630402035 6 19 7.222280723405998 6 20 6.604358590083738 6 21 7.026820236957114 6 22 7.829379134447084 6 23 6.435929482492572 6 24 8.038332776780724 6 25 6.73594474110477 6 26 5.839549507447163 6 27 5.790001235685114 6 28 5.903520068051275 6 29 6.484477149704112 6 30 6.160865573726333 6 31 5.648209596620809 6 32 5.219817002661394 6 33 5.488014450121032 6 34 5.063529387405776 6 35 4.692000567543875 6 36 6.515092007361638 6 37 6.433756514852501 6 38 7.385662441214343 6 39 7.892950978578664 6 40 6.278231894004314 6 41 10.964422054242497 6 42 10.426189928199495 6 43 10.708629417106543 6 44 11.34887287663938 6 45 10.663457693086904 6 46 11.52332793508878 6 47 12.112458285364081 6 48 12.352615994208499 6 49 12.530225748664261 6 50 12.197571768478142 6 51 11.826043914405002 6 52 11.450483923393545 6 53 11.57927933838156 6 54 12.027815387514256 6 55 11.799619757673472 6 56 12.38841275166077 7 1 1.7638300955566066 7 2 2.336355152257795 7 3 3.6596925308450707 7 4 2.6970963469066547 7 5 2.9672347899262306 7 6 3.4147984970569283 7 7 2.3613163781838935 7 8 2.2592647522241123 7 9 2.4478872593288727 7 10 2.702200662730057 7 11 3.8705423861437254 7 12 5.051981022239559 7 13 5.299338163235426 7 14 5.230808179845418 7 15 6.201889639323003 7 16 6.7050860506779735 7 17 7.949007212816699 7 18 8.153419256252374 7 19 6.225682083950215 7 20 6.826127297566732 7 21 6.826139964848267 7 22 7.373596821521918 7 23 6.6332083940571955 7 24 7.066904205352156 7 25 5.7536318159347 7 26 5.289889643501585 7 27 5.095443412555863 7 28 5.204200340160117 7 29 5.162708442221117 7 30 5.613926798216127 7 31 4.416917079614002 7 32 3.9429462543620772 7 33 4.260123293658442 7 34 3.968291292167681 7 35 4.427374717203737 7 36 5.315092007361642 7 37 4.777974201927325 7 38 6.221716862983051 7 39 6.34533193095962 7 40 6.834694479038326 7 41 10.54537443519488 7 42 9.724149111872965 7 43 9.058969553160964 7 44 9.486287842625774 7 45 8.93148490397126 7 46 10.353940179986736 7 47 11.213818829581765 7 48 10.494112592847955 7 49 10.658797177235687 7 50 10.116619387525766 7 51 10.133526907602278 7 52 10.413068957407145 7 53 10.225537841782923 7 54 10.182917428330583 7 55 10.083973499170071 7 56 8.82854880608254 8 1 2.197529729256239 8 2 2.740018155920801 8 3 3.842110113262656 8 4 2.9608326106429246 8 5 3.149652372343816 8 6 3.564249046507477 8 7 2.926884143751664 8 8 2.6365541295134847 8 9 2.9599751714167866 8 10 3.804032164561555 8 11 4.798747514348854 8 12 5.766266736525274 8 13 5.774796038693303 8 14 5.780258729295966 8 15 6.738153375586737 8 16 7.747576893168816 8 17 8.376113439922928 8 18 9.124115226948344 8 19 7.646927505195638 8 20 7.117702389141822 8 21 7.522110660818967 8 22 8.223413671338765 8 23 7.351157112005911 8 24 7.928442666890614 8 25 7.50674536904825 8 26 6.308937262549204 8 27 5.954051471163918 8 28 5.831306567266349 8 29 6.141463020975699 8 30 6.066674050963378 8 31 5.2007998634967905 8 32 5.341481052896874 8 33 5.805910839445989 8 34 5.206386530262922 8 35 5.370964460793477 8 36 5.954652446922083 8 37 6.15746138141451 8 38 6.893511734777924 8 39 8.162181747809438 8 40 3.020775065118915 8 41 11.700685790506235 8 42 10.818654606378455 8 43 10.941753435944847 8 44 11.849657805995736 8 45 10.519030691517045 8 46 13.136357762404323 8 47 13.138360954123893 8 48 12.94759244632781 8 49 14.43681915525767 8 50 13.18108825199463 8 51 12.108618482693853 8 52 12.405742950081137 8 53 12.053376669621748 8 54 12.749950395363552 8 55 12.548442363638937 8 56 11.41902499655873 9 1 1.4926469831363818 9 2 1.912103802233247 9 3 3.069707258385577 9 4 2.09768544852669 9 5 2.1695911964063512 9 6 2.774739586894924 9 7 1.6556708209919506 9 8 1.7766923418166485 9 9 1.9580492622743861 9 10 2.937939494345173 9 11 3.736325400380352 9 12 4.580699726215997 9 13 5.052013666426397 9 14 4.245535720346155 9 15 5.515880802798701 9 16 6.897231362410917 9 17 7.522595823518717 9 18 8.236384401073192 9 19 6.776983016694448 9 20 5.9735990697807715 9 21 6.337676538240515 9 22 6.901333689464973 9 23 6.596880460822046 9 24 7.412412305499426 9 25 6.032178698605296 9 26 4.9363304878805785 9 27 5.323033005093908 9 28 5.312104120228849 9 29 5.512143886501924 9 30 6.2748988158891805 9 31 4.809160574950283 9 32 4.611674776698848 9 33 4.694094820413479 9 34 4.667358547935972 9 35 4.765322679893966 9 36 5.503309974961049 9 37 5.851808271637683 9 38 6.68052884138266 9 39 6.965852304057311 9 40 5.679662569367242 9 41 9.899620876039258 9 42 8.693810378441444 9 43 8.959313195772651 9 44 10.30111356672985 9 45 8.657356283450888 9 46 10.816090400899846 9 47 10.75215952668535 9 48 10.413209303697244 9 49 11.120063745718749 9 50 10.211562931953843 9 51 10.014135645942975 9 52 9.292794043317802 9 53 10.18331889234748 9 54 9.291017575605988 9 55 9.729137956705664 9 56 9.112692154143414 10 1 2.0206753336518446 10 2 2.5845694379720783 10 3 3.5945139594165028 10 4 2.5795368230971363 10 5 3.1178300280214657 10 6 3.0192627827712144 10 7 2.313102092469613 10 8 2.433371895081251 10 9 2.564256306947925 10 10 3.666783996063387 10 11 4.143756671858014 10 12 5.120433403191939 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114 9 1.6544806659222786 114 10 2.3688673293967213 114 11 0.8690771846785239 114 12 -2.5106563403978015 114 13 -4.315313851416587 114 14 -3.951609402572167 114 15 -7.778330140896777 114 16 -2.815060469468545 114 17 0.12116838497787441 114 18 0.9570822599153779 114 19 -0.02999557172744005 114 20 0.264955136394569 114 21 3.9660667047750096 114 22 3.711325759250851 114 23 3.762146122994924 114 24 4.368003106451052 114 25 4.0935585558614385 114 26 3.174871328483274 114 27 3.147458064570511 114 28 3.8708670068267885 114 29 3.9700344495471285 114 30 4.068871853161184 114 31 3.189810852507776 114 32 2.1634590748748934 114 33 3.3289877625229103 114 34 3.2151777390541305 114 35 3.0566787465077674 114 36 4.196410688680324 114 37 5.082736106689236 114 38 5.152852394118582 114 39 5.452291637919327 114 40 7.893302537646385 114 41 8.896290186110626 114 42 6.836237023960873 114 43 6.007687501878916 114 44 -1.3569356005976694 114 45 7.086063658550014 114 46 2.771522597569156 114 47 -1.0154851997222636 114 48 1.4904495891849514 114 49 -6.745598427159916 114 50 2.19427506518144 114 51 3.750376724452096 114 52 3.915633059971249 114 53 3.6182118344569147 114 54 3.8202800656932183 114 55 4.436354451551026 114 56 4.871772249305984 115 1 0.9850239252011406 115 2 1.5525858698969657 115 3 2.6460104382897427 115 4 1.7413619404680247 115 5 1.9414802627632497 115 6 2.3005127827712144 115 7 1.4859307309672616 115 8 1.3270045241892348 115 9 1.8466800158681096 115 10 2.461825075875595 115 11 1.9891205216232706 115 12 -0.17739523530570978 115 13 -1.4923452707015272 115 14 -3.071002685345732 115 15 -7.460887020636754 115 16 2.8453945684512796 115 17 4.272414322139301 115 18 4.983734480933794 115 19 3.5851723589334448 115 20 3.7035250172179737 115 21 5.803336477256053 115 22 6.258238001937745 115 23 5.119459232420706 115 24 6.287829758672071 115 25 5.395617060736843 115 26 4.860245109631702 115 27 4.635215377679938 115 28 4.425796584291579 115 29 5.5613670605980445 115 30 5.11372559097267 115 31 4.599344980351766 115 32 3.9983453153949426 115 33 4.117936841612835 115 34 3.520269830061711 115 35 3.3544035569086326 115 36 4.629780136134279 115 37 5.4038628672526166 115 38 5.71319908967655 115 39 6.0187055057416465 115 40 8.564808496476289 115 41 9.081122255449742 115 42 8.424752733603345 115 43 7.434556407621059 115 44 -0.638192371995288 115 45 7.579238089752614 115 46 2.9281856528237604 115 47 -7.783632545334399 115 48 3.1229522977439963 115 49 -3.6558909515369464 115 50 3.3193021507719074 115 51 3.4403008847987913 115 52 3.844993840036256 115 53 3.8606820403074025 115 54 4.66621722712334 115 55 4.481424874086237 115 56 7.964296626337401 116 1 1.7378227695492825 116 2 2.240018155920801 116 3 3.303648574801116 116 4 2.3399534897638006 116 5 2.907894130585568 116 6 3.09538457764301 116 7 2.337140554008073 116 8 2.2226347155940758 116 9 2.544224255665874 116 10 3.122713483242876 116 11 3.225487441088781 116 12 2.52267699293553 116 13 4.179557943455208 116 14 3.520185469222703 116 15 -6.188586551153186 116 16 3.98750363309556 116 17 5.231424795234286 116 18 4.887851490684607 116 19 4.63410699237512 116 20 4.6470064184458515 116 21 5.630169268877573 116 22 6.70876165668675 116 23 5.131376892225692 116 24 6.111592850040797 116 25 5.975609837912717 116 26 5.2851277387396856 116 27 4.329509346621794 116 28 4.9759952119549915 116 29 5.044393423906101 116 30 4.920153904443232 116 31 4.818015980712907 116 32 5.107048818464641 116 33 4.536680070215223 116 34 4.415177739054126 116 35 4.374627464456484 116 36 6.491282483552119 116 37 5.780172004125131 116 38 6.334903676169866 116 39 7.03947112509881 116 40 9.493302537646386 116 41 7.570649160469603 116 42 5.595211382935233 116 43 1.802559296750708 116 44 0.519987476325408 116 45 4.5245251970115525 116 46 0.3433174693640275 116 47 -7.4513826356197 116 48 -1.345447846712485 116 49 -4.337906119467608 116 50 0.148121219027594 116 51 3.863197237272608 116 52 3.4387099830481738 116 53 3.1669297831748615 116 54 4.022844168257322 116 55 3.9953288105253826 116 56 6.184592762126496 117 1 0.5646371980586267 117 2 1.287606161135919 117 3 2.450975823823285 117 4 1.7721568795943057 117 5 1.0030701410158187 117 6 1.9784788844661279 117 7 1.3558280811701735 117 8 1.24392980468577 117 9 1.1181051770044235 117 10 2.0366639395662176 117 11 -0.9025003902888784 117 12 -8.165936653305234 117 13 -10.23786926210759 117 14 -2.5706446046582165 117 15 -6.702710844938498 117 16 -6.440353820185624 117 17 0.7696690368683576 117 18 -1.617363633174584 117 19 0.975480308324709 117 20 0.02532019506471528 117 21 4.263589520941892 117 22 5.171821196017479 117 23 4.55249814385542 117 24 4.735669338524065 117 25 4.543623744909677 117 26 4.604336778287706 117 27 4.3910043487947625 117 28 4.050528023775943 117 29 4.360125714214661 117 30 5.37082752460838 117 31 3.5783375800175534 117 32 3.5485959718762032 117 33 3.227032091075717 117 34 4.1361686125873725 117 35 4.054331940771132 117 36 5.030830506150984 117 37 4.153922547367198 117 38 5.207350438447136 117 39 6.482539356302635 117 40 8.237761468545997 117 41 5.724712611143222 117 42 4.687345237780953 117 43 -6.268714062658244 117 44 -2.5777960960344366 117 45 3.9671588345604434 117 46 -1.7559871807880825 117 47 -0.7351722922907129 117 48 -1.5757824838267815 117 49 -4.0569413215536585 117 50 -0.41380837680030425 117 51 4.682840870475566 117 52 4.373520934808276 117 53 3.8010019257215824 117 54 5.242965854480705 117 55 4.602456146466277 117 56 8.506192066776649 118 1 0.8773970317650495 118 2 1.4308744694186188 118 3 2.292037544322163 118 4 1.7267459425939862 118 5 1.855644493430276 118 6 2.092965612959894 118 7 1.4101932874381617 118 8 1.509040134074965 118 9 1.7055692000296858 118 10 1.836791857698607 118 11 -0.924246472796078 118 12 -7.422412508757745 118 13 -10.463644765785237 118 14 -9.575121739292051 118 15 -10.364687180084005 118 16 -10.094015476725438 118 17 -1.3972641259074656 118 18 -6.8879104831905735 118 19 -1.9045964425547268 118 20 -2.438383034868128 118 21 3.647343918127696 118 22 4.81205144865579 118 23 4.3331185467975395 118 24 5.356392075972099 118 25 4.5861565239311055 118 26 5.225379311066728 118 27 5.08591960303205 118 28 5.365206629468293 118 29 5.140426321825792 118 30 5.552471272609658 118 31 4.507662811869167 118 32 4.341688392726859 118 33 4.686897777036702 118 34 3.6430442122036233 118 35 3.938246235622426 118 36 5.091911414369726 118 37 5.788396484047727 118 38 6.689282002246308 118 39 6.368692218470851 118 40 9.414928081913438 118 41 7.740702377692632 118 42 7.655685500013124 118 43 1.543246282720716 118 44 2.1324693340902847 118 45 6.293030276837385 118 46 2.087632902358706 118 47 0.6840793866347745 118 48 2.8335555398380734 118 49 -0.4576448712818326 118 50 3.500806269825855 118 51 6.996240294843968 118 52 7.557723045457461 118 53 6.44027279236693 118 54 7.931165406767239 118 55 7.994845017588762 118 56 11.491801276882182 119 1 0.9342782748283156 119 2 1.296579241893646 119 3 2.6588521947106187 119 4 1.7958358427049745 119 5 1.8739574790018594 119 6 2.3887480768888594 119 7 1.1746217003127484 119 8 1.5883964048851738 119 9 1.7180553265557634 119 10 2.6012202705731937 119 11 1.1376292208776206 119 12 -5.924909734062961 119 13 -1.9003817247197539 119 14 -5.080116189902483 119 15 -8.54642968840809 119 16 -11.26438173643687 119 17 0.21483354334891303 119 18 -7.271650771758829 119 19 -0.8338417255735955 119 20 -0.9472620581755642 119 21 4.476700188937905 119 22 5.1880225918300376 119 23 4.044272819827501 119 24 3.590853785184084 119 25 4.5600743929655145 119 26 4.937088523053408 119 27 4.674154897149695 119 28 4.285572889179729 119 29 5.094921327375182 119 30 4.981994025106886 119 31 3.8447882280733907 119 32 4.209612921028743 119 33 4.189395002341914 119 34 3.6097478748007354 119 35 4.094461551937631 119 36 5.4079491502187835 119 37 4.794500812571588 119 38 5.7542098601819305 119 39 6.145051818914798 119 40 9.104162266153175 119 41 6.617783398780311 119 42 4.084653313553634 119 43 -3.4018147605645233 119 44 1.3638788790403389 119 45 5.064117957192547 119 46 -0.31580771917292694 119 47 -1.3944444757403645 119 48 -4.642582085023193 119 49 -1.9634717303273383 119 50 1.3404289113352874 119 51 6.198793014044856 119 52 5.540519937799303 119 53 6.266854368393567 119 54 5.92367373085159 119 55 6.298119157433376 119 56 8.07448718143268 120 1 0.7680270285670971 120 2 1.1520129407969364 120 3 2.2984334509419284 120 4 1.4670721338316 120 5 1.7996803105073411 120 6 1.9615297319237541 120 7 1.4744721489667825 120 8 1.3625738724823862 120 9 1.202850939716285 120 10 2.595985973464522 120 11 -1.2753817462210861 120 12 -10.454072246525573 120 13 -10.71244553329403 120 14 -7.299458163980251 120 15 -1.1603379635825668 120 16 -9.677641955778846 120 17 0.8883131046649666 120 18 -4.210583972157632 120 19 -1.4821468103193567 120 20 -0.46620522866409786 120 21 2.619521724331726 120 22 4.171821196017479 120 23 3.2982608557198283 120 24 3.8373642537783006 120 25 3.5944712025367984 120 26 4.977218134219907 120 27 4.950326382693067 120 28 4.491205989877631 120 29 4.98724435828246 120 30 5.184386846642276 120 31 4.391896902051457 120 32 4.633341734588065 120 33 4.464320226668935 120 34 4.288710985468729 120 35 4.596704822127062 120 36 5.624050845134036 120 37 6.594600513468894 120 38 6.020909760481032 120 39 7.296098678336538 120 40 7.966575027868032 120 41 -4.580372134619488 120 42 -3.1940106944224382 120 43 -4.455154740624344 120 44 -14.88288084179715 120 45 -2.9141970976429477 120 46 -7.332258367228759 120 47 -6.633477377036476 120 48 -4.880867229589494 120 49 -14.31117860968925 120 50 -3.8375371903596296 120 51 1.089620531492514 120 52 0.44131754497776754 120 53 1.173883281653783 120 54 1.0734743290569746 120 55 1.1617781803645855 120 56 4.5400903718613925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 983.0 5 101.0 6 196.0 7 289.0 8 399.0 9 477.0 10 350.0 11 310.0 12 250.0 13 152.0 14 180.0 15 227.0 16 252.0 17 316.0 18 428.0 19 546.0 20 753.0 21 968.0 22 1236.0 23 1396.0 24 1721.0 25 1938.0 26 2080.0 27 2299.0 28 2306.0 29 2578.0 30 2845.0 31 3226.0 32 3834.0 33 4148.0 34 4585.0 35 4957.0 36 5415.0 37 5887.0 38 6409.0 39 6319.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 100.0 0.0 7 99.38598860099208 0.430660772915275 0.09807126511932004 0.08527936097332177 8 99.44568415367341 0.35390934803928537 0.13218300950864875 0.06822348877865742 9 98.0897423141976 0.4178688687692767 1.0020324914365308 0.49035632559660014 10 57.21534459968447 15.958611083474281 17.317395568315874 9.508648748525378 11 37.441903435336926 21.77182085648905 14.15068863083986 26.635587077334165 12 31.630114985005047 20.62907741944654 19.472120755575137 28.268686839973277 13 24.04451582642807 13.025001065992011 21.069687451142034 41.86079565643788 14 22.689995309635147 26.53467316684907 25.23984820273747 25.53548332077832 15 34.05929564993859 18.3642283853646 22.391534089286225 25.184941875410583 16 30.881892898785658 25.34624463214174 23.04553081363934 20.726331655433267 17 27.606943318974707 16.958814915909638 33.69016647474446 21.744075290371192 18 25.347868726637007 17.72673650098782 25.600864164191194 31.324530608183977 19 30.433051835423242 20.75265148284 18.721032727686314 30.093263954050443 20 29.193059371130058 21.999060538339997 27.92763298364483 20.880247106885115 21 26.50340406782552 20.390295208721234 22.998422331821995 30.107878391631253 22 22.991315718407552 18.2071435678042 33.73793652372898 25.06360419005927 23 24.08004889350029 20.26237616726125 29.26361271799537 26.39396222124309 24 23.741559456962115 20.678086573317223 23.700334067808658 31.880019901912004 25 27.085998578535893 19.658848614072493 27.995735607675904 25.259417199715706 26 33.202036289175815 20.091291735396165 21.156361981684775 25.550309993743248 27 23.6703981337923 26.65177872921497 19.48565495071334 30.19216818627939 28 26.875133252789425 27.9482623836259 20.81728377514036 24.35932058844432 29 34.38523073419307 24.19364021977396 18.460158852165 22.960970193867965 30 31.37461247475753 20.038965840894225 27.597485707784635 20.98893597656361 31 33.12151711588764 25.19049243716593 20.952746502900034 20.7352439440464 32 36.750071083309635 23.268410577196473 20.477679840773387 19.503838498720498 33 32.99944510052217 23.429563336795525 21.75632798827597 21.814663574406328 34 24.2822966507177 22.311460469355207 34.10942128047391 19.296821599453178 35 23.57052367870411 23.52639744921925 34.956514312556045 17.94656455952059 36 27.86791996700843 26.494219365481154 25.086389556462507 20.55147111104791 37 29.996726491225573 22.23566416646504 27.79920581830603 19.96840352400336 38 33.190410393014346 25.02528454010627 22.36862722753237 19.41567783934702 39 31.594603057129035 22.68509293786115 18.978964447354187 26.74133955765563 40 24.576283327841374 17.157838937520594 24.814109084657375 33.45176864998066 41 21.259819902626777 15.8253026756093 22.092806158353557 40.82207126341036 42 23.322260083548382 25.664751422175964 20.73596715094313 30.277021343332528 43 18.87900558786635 21.259550689930435 28.399760520013682 31.461683202189533 44 21.202215433808206 23.35198561110001 25.227681502840664 30.21811745225112 45 26.922693408925795 21.090867651457955 27.462571758237065 24.523867181379185 46 20.83368985482445 26.536607626707738 24.106557143264595 28.523145375203217 47 18.790077262535526 23.922823153054086 23.130203796004057 34.15689578840633 48 25.094229593070395 21.756012914788002 25.162501600125164 27.987255892016442 49 21.781515000356304 23.87515142877503 23.367775956673555 30.97555761419511 50 23.526312041586557 23.81827244890693 24.926297799615465 27.72911770989105 51 21.747439883618107 25.58690133211627 26.01905468237442 26.646604101891207 52 21.089288003304326 27.44299326316389 27.374627907307968 24.09309082622381 53 21.27186417482787 25.785114541903663 28.39812399321444 24.544897290054028 54 22.435541112583916 21.916734368096236 29.161499978620604 26.48622454069925 55 20.14315657393809 22.551437982119687 28.816676885346414 28.48872855859581 56 22.685614105104122 21.207542866916576 28.833079148790603 27.2737638791887 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 2.0 10 6.5 11 11.0 12 23.0 13 44.5 14 54.0 15 61.0 16 68.0 17 76.0 18 84.0 19 88.0 20 92.0 21 101.5 22 102.0 23 93.0 24 130.5 25 168.0 26 167.5 27 167.0 28 189.0 29 208.5 30 206.0 31 290.0 32 374.0 33 385.5 34 397.0 35 484.0 36 571.0 37 633.0 38 742.5 39 790.0 40 883.0 41 976.0 42 1161.0 43 1346.0 44 1980.5 45 2615.0 46 4036.5 47 5503.0 48 5548.0 49 4708.0 50 3868.0 51 3809.0 52 3750.0 53 3782.0 54 4002.5 55 4191.0 56 4253.0 57 4315.0 58 4769.5 59 5224.0 60 5480.0 61 5736.0 62 5473.0 63 4608.5 64 4007.0 65 3524.5 66 3042.0 67 2526.5 68 2011.0 69 1833.0 70 1655.0 71 1483.0 72 1141.0 73 971.0 74 724.0 75 477.0 76 362.0 77 247.0 78 217.0 79 186.0 80 185.0 81 127.0 82 69.0 83 41.5 84 14.0 85 7.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.47614309876771327 16 0.044061003169549584 17 0.022741162926219142 18 0.0 19 0.027005130974885227 20 0.14781755902042443 21 0.0 22 0.0 23 0.0 24 0.017055872194664354 25 0.009949258780220873 26 0.046903648535326976 27 0.0767514248759896 28 0.0028426453657773927 29 0.1463962363375357 30 0.055431584632659146 31 0.018477194877553053 32 0.024162485609107835 33 0.10517787853376352 34 0.1890359168241966 35 0.14781755902042443 36 0.05116761658399306 37 0.13644697755731483 38 0.22172633853063659 39 0.13502565487442614 40 0.7930980570518924 41 1.034722913142971 42 0.3098483448697358 43 0.2913711499921827 44 0.430660772915275 45 0.22172633853063659 46 0.3340108304788436 47 0.47756442145060196 48 0.0710661341444348 49 0.27289395511462966 50 0.20182782097019486 51 0.3453814119419532 52 0.20751311170174963 53 0.29563511804084885 54 0.27857924584618443 55 0.31837628096706794 56 0.2814218912119618 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 70357.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.79648080503718 #Duplication Level Percentage of deduplicated Percentage of total 1 87.72661503106647 58.59829157013516 2 6.892075921355009 9.207328339752973 3 2.219337815984339 4.44731867475873 4 0.8702868329219509 2.3252839092059068 5 0.5553664141629074 1.8548261011697487 6 0.30215337475529835 1.210966925821169 7 0.28938633075155334 1.3530991941100388 8 0.1851221380543025 0.9892405872905327 9 0.11915907736828667 0.7163466321759029 >10 0.7319771895480467 9.289765055360519 >50 0.07234658268788834 3.247722330400671 >100 0.029789769342071667 3.5646772886848503 >500 0.0063835220018725 3.195133391133789 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCATCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 883 1.2550279289907187 No Hit CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 755 1.0730986255809656 No Hit CCATCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 610 0.8670068365621046 No Hit CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 280 0.39797035120883495 No Hit CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 277 0.39370638316016887 No Hit CCATCTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 269 0.3823358016970593 No Hit CCATCTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 241 0.3425387665761758 No Hit CCATCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 218 0.3098483448697358 No Hit CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 210 0.29847776340662624 No Hit CCATCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 191 0.27147263243174097 No Hit CCATCTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 136 0.19329988487286268 No Hit CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 133 0.1890359168241966 No Hit CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 119 0.16913739926375485 No Hit CCATCTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC 114 0.16203078584931138 No Hit CCATCTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 112 0.15918814048353397 No Hit CCATCTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 105 0.14923888170331312 No Hit CCATCTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 103 0.1463962363375357 No Hit CCATCTGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC 100 0.1421322682888696 No Hit CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 94 0.13360433219153744 No Hit CCATCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC 91 0.12934036414287137 No Hit CCATCTGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG 80 0.11370581463109569 No Hit CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 80 0.11370581463109569 No Hit CCATCTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 75 0.10659920121665221 No Hit CCATCTGGGGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA 75 0.10659920121665221 No Hit CCATCTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC 74 0.10517787853376352 No Hit CCATCTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT 73 0.10375655585087482 No Hit CCATCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC 73 0.10375655585087482 No Hit CCATCTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC 71 0.10091391048509743 No Hit CCATCTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 71 0.10091391048509743 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.017055872194664354 0.0 0.0 0.0 0.0 15 0.01847719487755305 0.0 0.0 0.0 0.0 16 0.019898517560441746 0.0 0.0 0.0 0.0 17 0.021319840243330442 0.0 0.0 0.0 0.0 18 0.021319840243330442 0.0 0.0 0.0 0.0 19 0.02984777634066262 0.0 0.0 0.0 0.0 20 0.031269099023551315 0.0 0.0 0.0 0.0 21 0.03411174438932871 0.0 0.0 0.0 0.0 22 0.0369543897551061 0.0 0.0 0.0 0.0 23 0.04121835780377219 0.0 0.0 0.0 0.0 24 0.04121835780377219 0.0 0.0 0.0 0.0 25 0.04548232585243828 0.0 0.0 0.0 0.0 26 0.04832497121821567 0.0 0.0 0.0 0.0 27 0.05258893926688176 0.0 0.0 0.0 0.0 28 0.058274229998436546 0.0 0.0 0.0 0.0 29 0.06253819804710263 0.0 0.0 0.0 0.0 30 0.06253819804710263 0.0 0.0 0.0 0.0 31 0.06822348877865742 0.0 0.0 0.0 0.0 32 0.07106613414443481 0.0 0.0 0.0 0.0 33 0.07106613414443481 0.0 0.0 0.0 0.0 34 0.07106613414443481 0.0 0.0 0.0 0.0 35 0.07248745682732351 0.0 0.0 0.0 0.0 36 0.0739087795102122 0.0 0.0 0.0 0.0 37 0.0753301021931009 0.0 0.0 0.0 0.0 38 0.0753301021931009 0.0 0.0 0.0 0.0 39 0.0753301021931009 0.0 0.0 0.0 0.0 40 0.0753301021931009 0.0 0.0 0.0 0.0 41 0.07817274755887829 0.0 0.0 0.0 0.0 42 0.08812200633909917 0.0 0.0 0.0 0.0 43 0.09664994243643134 0.0 0.0 0.0 0.0 44 0.10375655585087483 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGCTA 15 0.008214843 50.27977 40 TTGTCTA 15 0.008214843 50.27977 37 CGATTGA 30 9.946234E-7 50.27977 40 AGCGCTT 15 0.008214843 50.27977 37 AAGAGAC 20 4.0581689E-4 50.24373 35 ATGCTCG 20 4.0581689E-4 50.24373 36 TATGCCT 15 0.008238185 50.24373 35 AATACCT 20 4.0581689E-4 50.24373 35 TAGAGCG 15 0.008261575 50.207737 34 ATATGCA 15 0.008261575 50.207737 34 ACTTATC 15 0.008285016 50.1718 46 ATACTAT 15 0.008285016 50.1718 45 TACTATT 15 0.008285016 50.1718 46 TATTGTT 15 0.008308505 50.135906 43 TTATACT 15 0.008308505 50.135906 43 ATTATAC 15 0.008308505 50.135906 42 TATACTA 15 0.008308505 50.135906 44 TTATTGT 15 0.008308505 50.135906 42 TGTCAAA 15 0.008355632 50.064285 49 CAAAACT 20 4.1305448E-4 50.064285 49 >>END_MODULE