FastQCFastQC Report
Fri 17 Jun 2016
SRR1525881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7657
Sequences flagged as poor quality0
Sequence length40
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT440.5746375865221366No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC230.3003787384092987No Hit
CCATCTGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC170.22201906751991643No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT140.1828392320752253No Hit
CCATCTGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT130.1697792869269949No Hit
CCATCTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT100.13059945148230376No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG100.13059945148230376No Hit
CCATCTGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC90.1175395063340734No Hit
CCATCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA90.1175395063340734No Hit
CCATCTGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT90.1175395063340734No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC80.10447956118584303No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC80.10447956118584303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC251.8102003E-434.11184316
GCGATGG251.8102003E-434.11184318
CCAGTAG200.00257723834.11184326
TGCGATG200.00257723834.11184317
TGATCCT251.8102003E-434.11184318
AGTGGGT200.00257723834.11184328
TAAGAGC301.2709157E-534.11184334
TAGCATA301.2709157E-534.11184330
GCCAGTA200.00257723834.11184325
CTGGTTG301.2709157E-534.11184313
TTGATCC251.8102003E-434.11184317
GTAAGAG200.00257723834.11184333
ATATGCT301.2709157E-534.11184334
AGCATAT301.2709157E-534.11184331
GTGCGAT200.00257723834.11184316
TGGTGCG200.00257723834.11184314
CAGTGGG200.00257723834.11184327
CATATGC301.2709157E-534.11184333
GCATATG301.2709157E-534.11184332
GTAGCAT301.2709157E-534.11184329
Produced by FastQC (version 0.11.5)