FastQCFastQC Report
Fri 17 Jun 2016
SRR1525880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG60.5150214592274678No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC40.34334763948497854No Hit
CCATCTGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2575107296137339No Hit
CCATCTGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT30.2575107296137339No Hit
CCATCTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT30.2575107296137339No Hit
CCATCTGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTTT30.2575107296137339No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCCCC30.2575107296137339No Hit
CCATCTGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT20.17167381974248927No Hit
CCATCTGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTTT20.17167381974248927No Hit
CCATCTGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT20.17167381974248927No Hit
CCATCTGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17167381974248927No Hit
CCATCTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT20.17167381974248927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGGG200.001787722834.05
CTGGGAG200.001787722834.05
TCTGGGG352.9377952E-734.04
TCTGGGC200.001787722834.04
TCTGGGA401.4437319E-834.04
CATCTGG1150.034.02
CCATCTG1150.034.01
ATCTGGG1050.034.03
Produced by FastQC (version 0.11.5)