FastQCFastQC Report
Fri 17 Jun 2016
SRR1525879_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525879_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4614
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC861.8638925010836587No Hit
CCATCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG821.7771998266146511No Hit
CCATCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT781.6905071521456438No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT651.4087559601213697No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG400.8669267446900737No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA320.693541395752059No Hit
CCATCTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA150.3250975292587776No Hit
CCATCTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT140.30342436064152584No Hit
CCATCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA130.28175119202427396No Hit
CCATCTGGGGGGGAGGTAGTGACGAAAAATAACAATACAG130.28175119202427396No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC130.28175119202427396No Hit
CCATCTGGGGGGGCTGGTGAGCTGGCTCAGTGGGTAAGAG120.26007802340702213No Hit
CCATCTGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT120.26007802340702213No Hit
CCATCTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT110.23840485478977025No Hit
CCATCTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT100.21673168617251842No Hit
CCATCTGGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAG90.1950585175552666No Hit
CCATCTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG80.17338534893801474No Hit
CCATCTGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT80.17338534893801474No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC80.17338534893801474No Hit
CCATCTGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA80.17338534893801474No Hit
CCATCTGGGGGTTTTCATAGAGCTGAATCACATTAAAAAG70.15171218032076292No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG70.15171218032076292No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAG70.15171218032076292No Hit
CCATCTGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT70.15171218032076292No Hit
CCATCTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC70.15171218032076292No Hit
CCATCTGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCA60.13003901170351106No Hit
CCATCTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT60.13003901170351106No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAG60.13003901170351106No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGA60.13003901170351106No Hit
CCATCTGGGTCATAGATCCTCTTCTGAAGGAGATTGGGCG60.13003901170351106No Hit
CCATCTGGGGCTCCATCTGGGTCACAGATCCTCTTCTGAA60.13003901170351106No Hit
CCATCTGGGGCTCCATCTGGGTCACAGATCCTCTTCTGAG60.13003901170351106No Hit
CCATCTGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG50.10836584308625921No Hit
CCATCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATT50.10836584308625921No Hit
CCATCTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT50.10836584308625921No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC50.10836584308625921No Hit
CCATCTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT50.10836584308625921No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGGA200.002403732434.29670325
GAAGGAG200.002403732434.29670326
CTGGGGT358.260158E-733.9239165
CTGGGGC301.207467E-533.9239165
CTGGGGA405.6144927E-833.9239165
CTGGGCA200.002539170433.9239165
CTGGGAA200.002539170433.9239165
TGGGGGG800.033.9239166
TGGGGGA358.260158E-733.9239166
TGGGGAG251.7552891E-433.9239166
TCTGGGG2350.033.9239124
TCTGGGC453.788955E-933.9239124
CTGGGGG1350.032.6674735
CCATCTG4850.032.175261
ATCTGGG4700.032.119453
CATCTGG4850.031.8255272
TCTTCTG651.8189894E-1231.65849520
CTTCTGA651.8189894E-1231.65849521
TCTGGGA653.6379788E-1231.3143794
TCCTCTT604.7293724E-1131.09692217
Produced by FastQC (version 0.11.5)