##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525879_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4614
Sequences flagged as poor quality	0
Sequence length	40
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.169050715214563	33.0	30.0	35.0	4.0	38.0
2	24.206762028608583	29.0	14.0	34.0	4.0	37.0
3	19.09904638058084	19.0	4.0	30.0	4.0	35.0
4	21.82184655396619	25.0	4.0	33.0	4.0	36.0
5	20.806025140875597	25.0	4.0	33.0	4.0	36.0
6	18.983745123537062	20.0	4.0	31.0	4.0	35.0
7	18.245990463805807	20.0	4.0	30.0	4.0	35.0
8	18.08084091894235	19.0	4.0	31.0	4.0	35.0
9	17.06978760294755	15.0	4.0	30.0	4.0	35.0
10	17.298439531859557	15.0	4.0	31.0	4.0	35.0
11	14.810359774599046	4.0	4.0	28.0	4.0	33.0
12	13.78045080190724	4.0	4.0	26.0	4.0	32.0
13	13.17208495882098	4.0	4.0	24.0	4.0	31.0
14	13.080407455570004	4.0	4.0	25.0	4.0	33.0
15	12.540312093628089	4.0	4.0	24.0	4.0	33.0
16	10.244690073688773	4.0	4.0	16.0	4.0	29.0
17	9.705461638491547	4.0	4.0	14.0	4.0	27.0
18	9.68747290853923	4.0	4.0	14.0	4.0	27.0
19	9.058951018638925	4.0	4.0	10.0	4.0	26.0
20	9.244039878630256	4.0	4.0	11.0	4.0	26.0
21	8.963589076723016	4.0	4.0	9.0	4.0	27.0
22	8.940398786302557	4.0	4.0	10.0	4.0	26.0
23	9.14022540095362	4.0	4.0	10.0	4.0	27.0
24	8.925661031642827	4.0	4.0	10.0	4.0	26.0
25	9.154529692241006	4.0	4.0	11.0	4.0	27.0
26	9.246207195491982	4.0	4.0	10.0	4.0	28.0
27	9.195925444299956	4.0	4.0	10.0	4.0	28.0
28	9.323146944083225	4.0	4.0	9.0	4.0	29.0
29	8.58777633289987	4.0	4.0	4.0	4.0	26.0
30	9.260511486779366	4.0	4.0	4.0	4.0	28.0
31	9.720849588209797	4.0	4.0	4.0	4.0	31.0
32	9.5765062852189	4.0	4.0	4.0	4.0	31.0
33	9.430429128738622	4.0	4.0	4.0	4.0	30.0
34	9.439965322930213	4.0	4.0	4.0	4.0	31.0
35	9.255526657997398	4.0	4.0	4.0	4.0	30.0
36	9.063285652362376	4.0	4.0	4.0	4.0	31.0
37	8.830732553099264	4.0	4.0	4.0	4.0	30.0
38	8.73667100130039	4.0	4.0	4.0	4.0	30.0
39	8.481361074989163	4.0	4.0	4.0	4.0	29.0
40	8.431512787169485	4.0	4.0	4.0	4.0	29.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-23.95425117953208
1	2	-18.72546167939426
1	3	-13.156110057233654
1	4	-16.59940496457351
1	5	-15.170072717544624
1	6	-13.908227134912526
1	7	-13.368643902913568
1	8	-12.96650291032314
1	9	-12.007370656808856
1	10	-12.257316341586005
1	11	-8.708753668304231
1	12	-8.24785292734731
1	13	-7.459004166869336
1	14	-7.450633137149989
1	15	-6.902341229307519
1	16	-5.304856348395674
1	17	-5.087550059179273
1	18	-5.376137376980074
1	19	-4.890724662353875
1	20	-4.831052904648409
1	21	-4.477696710280981
1	22	-4.572030497592294
1	23	-4.8129039188027924
1	24	-4.510540395933656
1	25	-4.891761920975407
1	26	-4.884164680512992
1	27	-4.675658268073997
1	28	-4.6504734341813005
1	29	-3.9441327399192563
1	30	-4.623734536374984
1	31	-4.902842631775217
1	32	-4.76216782593187
1	33	-4.600080662159312
1	34	-4.6423115586036925
1	35	-4.361158130259254
1	36	-4.345265658187007
1	37	-4.039299090422684
1	38	-3.8304521944971377
1	39	-3.471268868459881
1	40	-3.883494657651961
2	1	7.3790821538012565
2	2	2.941204987272407
2	3	10.177223276099678
2	4	4.567261702093159
2	5	9.663260615788708
2	6	5.591772865087474
2	7	-0.7019772362469006
2	8	-0.7998362436564719
2	9	-4.174037323475522
2	10	7.076016991747327
2	11	-0.8754203349708973
2	12	8.25214707265269
2	13	4.20766249979733
2	14	7.382700196183343
2	15	5.097658770692481
2	16	5.361810318270992
2	17	2.579116607487393
2	18	8.457195956353258
2	19	9.109275337646125
2	20	5.335613762018257
2	21	10.355636623052352
2	22	6.427969502407706
2	23	7.6870960811972076
2	24	2.8227929373996776
2	25	13.274904745691261
2	26	5.449168652820342
2	27	7.324341731926003
2	28	7.682859899152032
2	29	7.72253392674741
2	30	10.209598796958348
2	31	13.430490701558115
2	32	14.73783217406813
2	33	12.566586004507355
2	34	12.69102177472964
2	35	10.638841869740746
2	36	19.154734341812993
2	37	17.960700909577316
2	38	18.502881138836194
2	39	13.52873113154012
2	40	12.616505342348038
3	1	-4.732028957309858
3	2	-7.5032394571720396
3	3	-4.933887835011433
3	4	-2.8216271867957303
3	5	-4.392294939766845
3	6	-5.24156046824586
3	7	-4.701977236246902
3	8	-4.96650291032314
3	9	-4.896259545697745
3	10	-7.923983008252671
3	11	-2.5976425571931205
3	12	-3.0256307051250886
3	13	-1.6812263890915577
3	14	-2.117299803816655
3	15	-4.457896784863074
3	16	-2.415967459506785
3	17	-0.8653278369570518
3	18	-1.4872484880911845
3	19	-2.5573913290205423
3	20	-0.49771957131507527
3	21	-2.2554744880587583
3	22	-0.016474942036738938
3	23	0.18709608119720755
3	24	-1.5105403959336563
3	25	-2.113984143197629
3	26	-1.217498013846325
3	27	0.8798972874815583
3	28	1.2384154547075887
3	29	1.2780894823029652
3	30	0.7095987969583497
3	31	0.7638240348914493
3	32	-0.9843900481540926
3	33	0.8443637822851322
3	34	2.0243551080629736
3	35	1.4166196475185249
3	36	-0.900821213742562
3	37	-1.5948546459782396
3	38	-1.386007750052693
3	39	-2.915713312904326
3	40	-0.6612724354297388
4	1	5.3314631061822055
4	2	4.417395463462881
4	3	7.843889942766346
4	4	5.257737892569349
4	5	1.829927282455376
4	6	-0.7653699920553834
4	7	5.917070382800716
4	8	8.747782803962576
4	9	10.421200771762571
4	10	2.45696937269971
4	11	12.005532045981484
4	12	11.466432786938405
4	13	8.826710118844948
4	14	4.120795434278582
4	15	8.383373056406766
4	16	17.98085793731861
4	17	10.912449940820727
4	18	14.1952911944485
4	19	11.680703909074698
4	20	17.02608995249445
4	21	15.808017575433304
4	22	12.856540930979133
4	23	13.187096081197208
4	24	14.346602461209201
4	25	14.53680950759602
4	26	11.544406748058435
4	27	13.038627446211718
4	28	10.492383708675842
4	29	12.913010117223601
4	30	13.947694035053589
4	31	12.52572879679621
4	32	9.809260745496703
4	33	12.257062194983545
4	34	12.92911701282488
4	35	14.353127584026462
4	36	12.654734341812993
4	37	13.389272338148743
4	38	15.740976376931435
4	39	10.243016845825835
4	40	13.545076770919465
5	1	-10.95425117953208
5	2	-18.72546167939426
5	3	-13.156110057233654
5	4	-16.59940496457351
5	5	-15.170072717544624
5	6	-13.908227134912526
5	7	-13.368643902913568
5	8	-12.96650291032314
5	9	-12.007370656808856
5	10	-12.257316341586005
5	11	-8.708753668304231
5	12	-8.24785292734731
5	13	-7.459004166869336
5	14	-7.450633137149989
5	15	-6.902341229307519
5	16	-5.304856348395674
5	17	-5.087550059179273
5	18	-5.376137376980074
5	19	-4.890724662353875
5	20	-4.831052904648409
5	21	-4.477696710280981
5	22	-4.572030497592294
5	23	-4.8129039188027924
5	24	-4.510540395933656
5	25	-4.891761920975407
5	26	-4.884164680512992
5	27	-4.675658268073997
5	28	-4.6504734341813005
5	29	-3.9441327399192563
5	30	-4.623734536374984
5	31	-4.902842631775217
5	32	-4.76216782593187
5	33	-4.600080662159312
5	34	-4.6423115586036925
5	35	-4.361158130259254
5	36	-4.345265658187007
5	37	-4.039299090422684
5	38	-3.8304521944971377
5	39	-3.471268868459881
5	40	-3.883494657651961
6	1	5.156859931579035
6	2	7.38564943171685
6	3	8.621667720544124
6	4	4.845039479870934
6	5	1.0521495046775975
6	6	-6.574893801579192
6	7	3.853578319308653
6	8	3.9223859785657496
6	9	2.2148515654133654
6	10	2.4093503250806627
6	11	4.069024109473547
6	12	3.8632581837638007
6	13	3.5409958331306637
6	14	4.882700196183343
6	15	9.319880992914703
6	16	5.695143651604326
6	17	7.579116607487395
6	18	10.179418178575483
6	19	6.220386448757235
6	20	4.835613762018257
6	21	8.188969956385687
6	22	7.205747280185484
6	23	6.853762747863874
6	24	11.933904048510787
6	25	6.330460301246815
6	26	9.33805754170923
6	27	7.213230620814892
6	28	7.905082121374257
6	29	5.055867260080744
6	30	8.931821019180573
6	31	9.541601812669226
6	32	8.571165507401462
6	33	8.511030448951798
6	34	12.357688441396308
6	35	10.194397425296303
6	36	9.21028989736855
6	37	11.738478687355094
6	38	10.83621447216953
6	39	9.084286687095677
6	40	7.22761645345915
7	1	5.920748820467921
7	2	-1.350461679394261
7	3	11.093889942766346
7	4	5.900595035426491
7	5	8.204927282455376
7	6	-2.1582271349125257
7	7	5.006356097086432
7	8	13.90849708967686
7	9	-5.382370656808856
7	10	12.117683658413995
7	11	-0.8337536683042313
7	12	1.37714707265269
7	13	3.6659958331306637
7	14	0.424366862850011
7	15	-2.6523412293075186
7	16	2.570143651604326
7	17	2.1624499408207267
7	18	-0.5011373769800738
7	19	7.234275337646125
7	20	-2.206052904648409
7	21	9.77230328971902
7	22	5.427969502407706
7	23	3.5620960811972076
7	24	2.8644596040663437
7	25	4.858238079024593
7	26	8.990835319487008
7	27	2.574341731926003
7	28	3.8495265658186995
7	29	6.430867260080744
7	30	7.876265463625016
7	31	5.972157368224783
7	32	4.11283217406813
7	33	5.649919337840688
7	34	3.9826884413963075
7	35	5.138841869740746
7	36	5.404734341812993
7	37	4.335700909577316
7	38	3.6695478055028623
7	39	4.153731131540119
7	40	1.8665053423480389
8	1	6.045748820467921
8	2	-7.892128346060927
8	3	-2.1561100572336542
8	4	-5.599404964573509
8	5	8.496593949122044
8	6	-3.574893801579192
8	7	1.7980227637530994
8	8	-0.6331695769898076
8	9	-2.507370656808856
8	10	5.076016991747327
8	11	6.457912998362437
8	12	-1.414519594013976
8	13	4.7076624997973315
8	14	-0.6172998038166551
8	15	-6.402341229307519
8	16	-4.471523015062341
8	17	-4.25421672584594
8	18	-4.376137376980074
8	19	-2.0573913290205423
8	20	-2.831052904648409
8	21	-1.9776967102809806
8	22	-3.5720304975922943
8	23	-2.3129039188027924
8	24	-4.010540395933656
8	25	-1.0584285876420738
8	26	-1.5508313471796589
8	27	-3.675658268073997
8	28	-1.4838067675146336
8	29	-2.9441327399192563
8	30	-2.4570678697083173
8	31	-4.069509298441884
8	32	-3.7621678259318703
8	33	-0.2667473288259785
8	34	-1.3089782252703595
8	35	-1.8611581302592537
8	36	0.32140100847965947
8	37	1.2940342429106497
8	38	1.0028811388361962
8	39	0.8620644648734528
8	40	1.4498386756813728
9	1	8.500294275013374
9	2	10.819992866060286
9	3	15.207526306402713
9	4	12.946049580881038
9	5	14.739018191546283
9	6	16.273591046905654
9	7	16.085901551631885
9	8	14.306224362404134
9	9	16.083538434100237
9	10	14.651774567504905
9	11	11.563973604423042
9	12	5.206692527198143
9	13	6.995541287676117
9	14	0.549366862850011
9	15	10.188567861601571
9	16	1.8769618334225076
9	17	3.6397226680934534
9	18	1.714771713929018
9	19	2.8365480649188513
9	20	4.714401640806136
9	21	3.3404851079008377
9	22	6.427969502407706
9	23	6.278005172106299
9	24	4.489459604066344
9	25	7.4718744426609565
9	26	7.570380774032461
9	27	4.96070536828964
9	28	8.531344747636883
9	29	4.146776350989835
9	30	7.558083645443199
9	31	4.642611913679328
9	32	6.23783217406813
9	33	5.490828428749779
9	34	6.994052077759944
9	35	8.184296415195293
9	36	5.836552523631175
9	37	6.960700909577316
9	38	4.987729623684681
9	39	6.7105493133583005
9	40	5.2983235241662205
10	1	7.045748820467921
10	2	12.274538320605739
10	3	15.843889942766346
10	4	9.400595035426491
10	5	13.829927282455376
10	6	14.091772865087474
10	7	19.63135609708643
10	8	20.03349708967686
10	9	13.992629343191144
10	10	15.742683658413995
10	11	-0.7087536683042313
10	12	14.75214707265269
10	13	2.5409958331306637
10	14	-1.450633137149989
10	15	22.097658770692483
10	16	-1.304856348395674
10	17	15.912449940820727
10	18	-1.3761373769800738
10	19	16.109275337646125
10	20	19.16894709535159
10	21	1.5223032897190194
10	22	13.427969502407706
10	23	17.187096081197208
10	24	9.489459604066344
10	25	18.108238079024595
10	26	23.115835319487008
10	27	22.324341731926005
10	28	22.3495265658187
10	29	7.055867260080744
10	30	3.376265463625016
10	31	7.097157368224783
10	32	4.23783217406813
10	33	8.399919337840688
10	34	9.357688441396308
10	35	10.638841869740746
10	36	23.654734341812993
10	37	23.960700909577316
10	38	28.169547805502862
10	39	25.52873113154012
10	40	25.116505342348038
11	1	9.04574882046792
11	2	13.774538320605739
11	3	1.8438899427663458
11	4	7.400595035426491
11	5	3.329927282455376
11	6	18.591772865087474
11	7	15.131356097086432
11	8	6.53349708967686
11	9	8.492629343191144
11	10	14.742683658413995
11	11	12.791246331695769
11	12	11.25214707265269
11	13	10.540995833130664
11	14	9.549366862850011
11	15	11.097658770692481
11	16	0.1951436516043259
11	17	15.412449940820727
11	18	12.123862623019926
11	19	5.109275337646125
11	20	11.66894709535159
11	21	12.02230328971902
11	22	16.927969502407706
11	23	9.687096081197208
11	24	14.489459604066344
11	25	14.108238079024593
11	26	23.115835319487008
11	27	18.324341731926005
11	28	15.3495265658187
11	29	24.055867260080745
11	30	21.876265463625018
11	31	18.597157368224785
11	32	15.23783217406813
11	33	10.899919337840688
11	34	13.857688441396308
11	35	14.638841869740746
11	36	17.154734341812993
11	37	13.460700909577316
11	38	7.669547805502862
11	39	15.02873113154012
11	40	17.616505342348038
12	1	-4.579251179532079
12	2	0.899538320605739
12	3	-1.1561100572336542
12	4	-9.724404964573509
12	5	-10.420072717544624
12	6	-6.408227134912526
12	7	3.8813560970864316
12	8	4.28349708967686
12	9	4.867629343191144
12	10	5.617683658413995
12	11	9.791246331695769
12	12	10.25214707265269
12	13	8.790995833130664
12	14	10.174366862850011
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105	9	1.492629343191144
105	10	11.909350325080663
105	11	1.9579129983624348
105	12	2.585480405986024
105	13	-1.2923375002026702
105	14	5.716033529516679
105	15	-1.4023412293075186
105	16	2.02847698493766
105	17	6.079116607487393
105	18	1.1238626230199262
105	19	3.1092753376461246
105	20	-2.997719571315076
105	21	1.3556366230523533
105	22	2.4279695024077057
105	23	1.0204294145305415
105	24	2.1561262707330098
105	25	3.7749047456912592
105	26	0.28250198615367417
105	27	0.6576750652593368
105	28	0.6828598991520334
105	29	1.3892005934140776
105	30	0.5429321302916819
105	31	-4.902842631775217
105	32	-4.76216782593187
105	33	-4.600080662159312
105	34	-4.6423115586036925
105	35	-4.361158130259254
105	36	-4.345265658187007
105	37	-4.039299090422684
105	38	-3.8304521944971377
105	39	-3.471268868459881
105	40	-3.883494657651961
106	1	6.845748820467918
106	2	10.474538320605742
106	3	10.643889942766346
106	4	7.80059503542649
106	5	2.829927282455376
106	6	18.291772865087477
106	7	10.83135609708643
106	8	11.433497089676859
106	9	11.592629343191145
106	10	16.542683658413992
106	11	10.09124633169577
106	12	13.152147072652689
106	13	12.340995833130664
106	14	14.549366862850011
106	15	11.097658770692481
106	16	10.095143651604324
106	17	4.112449940820726
106	18	7.623862623019926
106	19	1.9092753376461253
106	20	-1.4310529046484088
106	21	-0.2776967102809813
106	22	-0.17203049759229394
106	23	-0.2129039188027928
106	24	-0.11054039593365594
106	25	0.30823807902459244
106	26	-4.884164680512992
106	27	-4.675658268073997
106	28	-4.6504734341813005
106	29	-3.9441327399192563
106	30	-4.623734536374984
106	31	-4.902842631775217
106	32	-4.76216782593187
106	33	-4.600080662159312
106	34	-4.6423115586036925
106	35	-4.361158130259254
106	36	-4.345265658187007
106	37	-4.039299090422684
106	38	-3.8304521944971377
106	39	-3.471268868459881
106	40	-3.883494657651961
107	1	5.170748820467921
107	2	6.274538320605739
107	3	-1.6561100572336542
107	4	6.650595035426491
107	5	7.454927282455376
107	6	0.3417728650874743
107	7	-5.118643902913568
107	8	-4.84150291032314
107	9	-2.132370656808856
107	10	4.992683658413995
107	11	7.041246331695769
107	12	5.62714707265269
107	13	7.665995833130664
107	14	6.799366862850011
107	15	-6.152341229307519
107	16	-2.554856348395674
107	17	-1.7125500591792733
107	18	-2.6261373769800738
107	19	-1.5157246623538754
107	20	-1.4560529046484092
107	21	-1.3526967102809806
107	22	-0.6970304975922943
107	23	-2.3129039188027924
107	24	-3.1355403959336563
107	25	-1.0167619209754069
107	26	-3.634164680512992
107	27	-3.675658268073997
107	28	-4.2754734341813005
107	29	-0.8191327399192563
107	30	-1.1237345363749842
107	31	-4.027842631775217
107	32	-4.13716782593187
107	33	-3.7250806621593124
107	34	-2.6423115586036925
107	35	-1.2361581302592537
107	36	-2.0952656581870066
107	37	-0.1642990904226842
107	38	-0.2054521944971377
107	39	-1.7212688684598811
107	40	-1.1334946576519611
108	1	8.17074882046792
108	2	11.899538320605739
108	3	15.218889942766346
108	4	12.275595035426491
108	5	13.204927282455376
108	6	6.466772865087474
108	7	3.8813560970864316
108	8	2.9084970896768603
108	9	10.992629343191144
108	10	12.117683658413995
108	11	-3.2087536683042313
108	12	0.87714707265269
108	13	0.16599583313066368
108	14	3.299366862850011
108	15	0.9726587706924814
108	16	3.570143651604326
108	17	4.787449940820727
108	18	-2.8761373769800738
108	19	-2.1407246623538754
108	20	-1.4560529046484092
108	21	-1.4776967102809806
108	22	-1.5720304975922943
108	23	-2.3129039188027924
108	24	-1.5105403959336563
108	25	-2.141761920975407
108	26	-4.134164680512992
108	27	-1.300658268073997
108	28	-2.4004734341813005
108	29	-0.9441327399192563
108	30	-1.6237345363749842
108	31	-1.4028426317752167
108	32	-2.6371678259318703
108	33	-1.1000806621593124
108	34	-1.3923115586036925
108	35	-1.8611581302592537
108	36	-1.0952656581870066
108	37	-4.039299090422684
108	38	-3.8304521944971377
108	39	-3.471268868459881
108	40	-3.883494657651961
109	1	6.188605963325067
109	2	7.988824034891454
109	3	12.272461371337773
109	4	4.400595035426491
109	5	3.1156415681696608
109	6	11.234630007944617
109	7	3.9170703828007163
109	8	16.747782803962576
109	9	13.135486486048286
109	10	11.742683658413995
109	11	10.291246331695769
109	12	8.323575644081263
109	13	9.398138690273521
109	14	6.406509719992869
109	15	7.097658770692481
109	16	12.409429365890041
109	17	2.198164226535013
109	18	6.481005480162784
109	19	2.252132480503267
109	20	4.311804238208733
109	21	9.093731861147592
109	22	6.8565409309791345
109	23	11.044238938340065
109	24	6.346602461209201
109	25	10.251095221881735
109	26	2.115835319487008
109	27	2.7529131604974317
109	28	1.7780979943901283
109	29	4.627295831509315
109	30	2.6619797493393023
109	31	3.2400145110819256
109	32	3.523546459782416
109	33	3.54277648069783
109	34	0.07197415568202103
109	35	-0.7897295588306825
109	36	0.08330577038442222
109	37	-2.32501337613697
109	38	0.31240494836000465
109	39	-2.328411725602738
109	40	0.25936248520518124
110	1	7.045748820467921
110	2	12.274538320605739
110	3	2.3438899427663458
110	4	13.400595035426491
110	5	12.329927282455376
110	6	-13.908227134912526
110	7	-13.368643902913568
110	8	-12.96650291032314
110	9	-12.007370656808856
110	10	-12.257316341586005
110	11	-8.708753668304231
110	12	-8.24785292734731
110	13	-7.459004166869336
110	14	-7.450633137149989
110	15	-6.902341229307519
110	16	-5.304856348395674
110	17	-5.087550059179273
110	18	-5.376137376980074
110	19	-4.890724662353875
110	20	-4.831052904648409
110	21	-4.477696710280981
110	22	-4.572030497592294
110	23	-4.8129039188027924
110	24	-4.510540395933656
110	25	-4.891761920975407
110	26	-4.884164680512992
110	27	-4.675658268073997
110	28	-4.6504734341813005
110	29	-3.9441327399192563
110	30	-4.623734536374984
110	31	-4.902842631775217
110	32	-4.76216782593187
110	33	-4.600080662159312
110	34	-4.6423115586036925
110	35	-4.361158130259254
110	36	-4.345265658187007
110	37	-4.039299090422684
110	38	-3.8304521944971377
110	39	-3.471268868459881
110	40	-3.883494657651961
111	1	3.24574882046792
111	2	7.07453832060574
111	3	-6.956110057233655
111	4	-0.9994049645735075
111	5	3.4299272824553775
111	6	-0.7082271349125264
111	7	2.03135609708643
111	8	2.433497089676859
111	9	8.392629343191143
111	10	-4.257316341586005
111	11	3.89124633169577
111	12	1.75214707265269
111	13	-0.6590041668693356
111	14	4.549366862850011
111	15	2.897658770692482
111	16	8.895143651604325
111	17	6.312449940820727
111	18	6.423862623019927
111	19	0.10927533764612463
111	20	-0.031052904648408486
111	21	1.3223032897190201
111	22	2.627969502407705
111	23	5.587096081197208
111	24	-0.11054039593365594
111	25	-3.6917619209754067
111	26	0.9158353194870088
111	27	0.12434173192600362
111	28	-3.050473434181301
111	29	0.4558672600807441
111	30	0.1762654636250165
111	31	0.897157368224784
111	32	1.0378321740681304
111	33	-2.8000806621593126
111	34	1.1576884413963082
111	35	-4.361158130259254
111	36	-4.345265658187007
111	37	-4.039299090422684
111	38	-3.8304521944971377
111	39	-3.471268868459881
111	40	-3.883494657651961
112	1	-23.95425117953208
112	2	-18.72546167939426
112	3	-13.156110057233654
112	4	-16.59940496457351
112	5	-15.170072717544624
112	6	-13.908227134912526
112	7	-13.368643902913568
112	8	-12.96650291032314
112	9	-12.007370656808856
112	10	-12.257316341586005
112	11	-8.708753668304231
112	12	-8.24785292734731
112	13	-7.459004166869336
112	14	-7.450633137149989
112	15	-6.902341229307519
112	16	-5.304856348395674
112	17	-5.087550059179273
112	18	-5.376137376980074
112	19	-4.890724662353875
112	20	-4.831052904648409
112	21	-4.477696710280981
112	22	-4.572030497592294
112	23	-4.8129039188027924
112	24	-4.510540395933656
112	25	-4.891761920975407
112	26	-4.884164680512992
112	27	-4.675658268073997
112	28	-4.6504734341813005
112	29	-3.9441327399192563
112	30	-4.623734536374984
112	31	-4.902842631775217
112	32	-4.76216782593187
112	33	-4.600080662159312
112	34	-4.6423115586036925
112	35	-4.361158130259254
112	36	-4.345265658187007
112	37	-4.039299090422684
112	38	-3.8304521944971377
112	39	-3.471268868459881
112	40	-3.883494657651961
117	1	6.712415487134585
117	2	10.941204987272403
117	3	3.5105566094330136
117	4	3.7339283687598233
117	5	4.163260615788708
117	6	1.4251061984208064
117	7	-3.0353105695802345
117	8	6.700163756343528
117	9	7.992629343191144
117	10	5.076016991747327
117	11	-0.04208700163756518
117	12	-0.5811862606806439
117	13	-1.4590041668693363
117	14	-0.11729980381665506
117	15	2.7643254373591475
117	16	-5.304856348395674
117	17	-5.087550059179273
117	18	-5.376137376980074
117	19	-4.890724662353875
117	20	-4.831052904648409
117	21	-4.477696710280981
117	22	-4.572030497592294
117	23	-4.8129039188027924
117	24	-4.510540395933656
117	25	-4.891761920975407
117	26	-4.884164680512992
117	27	-4.675658268073997
117	28	-4.6504734341813005
117	29	-3.9441327399192563
117	30	-4.623734536374984
117	31	-4.902842631775217
117	32	-4.76216782593187
117	33	-4.600080662159312
117	34	-4.6423115586036925
117	35	-4.361158130259254
117	36	-4.345265658187007
117	37	-4.039299090422684
117	38	-3.8304521944971377
117	39	-3.471268868459881
117	40	-3.883494657651961
119	1	8.54574882046792
119	2	13.274538320605739
119	3	14.343889942766346
119	4	16.90059503542649
119	5	14.829927282455376
119	6	13.591772865087474
119	7	15.131356097086432
119	8	0.03349708967686027
119	9	-0.5073706568088561
119	10	2.242683658413995
119	11	-3.7087536683042313
119	12	2.75214707265269
119	13	7.540995833130664
119	14	6.549366862850011
119	15	-6.902341229307519
119	16	-5.304856348395674
119	17	-5.087550059179273
119	18	-5.376137376980074
119	19	-4.890724662353875
119	20	-4.831052904648409
119	21	-4.477696710280981
119	22	-4.572030497592294
119	23	-4.8129039188027924
119	24	-4.510540395933656
119	25	-4.891761920975407
119	26	-4.884164680512992
119	27	-4.675658268073997
119	28	-4.6504734341813005
119	29	-3.9441327399192563
119	30	-4.623734536374984
119	31	-4.902842631775217
119	32	-4.76216782593187
119	33	-4.600080662159312
119	34	-4.6423115586036925
119	35	-4.361158130259254
119	36	-4.345265658187007
119	37	-4.039299090422684
119	38	-3.8304521944971377
119	39	-3.471268868459881
119	40	-3.883494657651961
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1008.0
5	225.0
6	187.0
7	282.0
8	216.0
9	229.0
10	300.0
11	275.0
12	188.0
13	175.0
14	128.0
15	81.0
16	66.0
17	56.0
18	40.0
19	57.0
20	92.0
21	125.0
22	153.0
23	154.0
24	133.0
25	111.0
26	100.0
27	83.0
28	73.0
29	40.0
30	22.0
31	10.0
32	4.0
33	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.00238559965301	0.32530904359141183	0.3903708523096942	0.2819345044458903
8	98.74159253634194	0.585810371013235	0.32545020611846387	0.3471468865263615
9	97.4609375	0.4991319444444444	1.5842013888888888	0.4557291666666667
10	56.36521361960529	16.52569941444372	17.198004771199308	9.91108219475168
11	38.94668400520156	21.1530125704378	15.821413090593845	24.078890333766797
12	35.602775368603645	23.113616652211622	20.09973980919341	21.183868169991328
13	25.26064291920069	17.723718505647263	22.046046915725455	34.969591659426584
14	21.39141742522757	31.079323797139143	28.218465539661896	19.310793237971392
15	35.99913307325531	22.756827048114435	24.989163415691372	16.25487646293888
16	34.22193324664066	27.37321196358908	24.664065886432596	13.740788903337666
17	28.088426527958386	21.60814911140009	35.50065019505852	14.802774165583008
18	27.61161681837885	21.673168617251843	27.0914607715648	23.623753792804507
19	29.03015838576698	26.057713169885005	21.17596007810805	23.736168366239966
20	29.34547030775899	25.249241439098398	29.887299523190293	15.51798872995232
21	25.53099263112267	25.097529258777634	25.20589510186389	24.165583008235807
22	21.824880797572604	20.71954919809276	37.19115734720416	20.264412657130475
23	23.347062649035337	22.978538911771082	32.75525688272274	20.919141556470844
24	25.35760728218466	22.756827048114435	27.264846120502817	24.620719549198093
25	27.16424387068778	22.130614016055546	28.596224777609024	22.108917335647646
26	30.036948489458815	23.38622038687242	23.125407520104325	23.451423603564443
27	18.81661953447901	30.998477267783336	23.40656950184903	26.778333695888623
28	23.520485584218513	33.34055928896596	21.41773249512248	21.721222631693042
29	31.617967727867423	27.976450065416486	22.590492804186653	17.815089402529438
30	28.094515499674834	25.1896813353566	29.04834164318231	17.667461521786258
31	30.529054640069386	28.707718993928882	24.045967042497832	16.717259323503903
32	35.00217675228559	24.531998258598172	23.421854592947323	17.043970396168916
33	31.772865192891203	25.79107065452969	24.1222366710013	18.313827481577807
34	21.08799306458604	25.42262678803641	37.79800606848721	15.691374078890332
35	19.007368877329867	28.413524057217167	37.971391417425224	14.607715648027742
36	21.92582953806116	33.59358056820646	25.80785079158534	18.67273910214704
37	26.57130472475076	29.19375812743823	27.65496315561335	16.579973992197658
38	30.775899436497618	28.738621586475944	23.75379280450802	16.731686172518422
39	27.77536860364267	27.797051170858627	20.121422376409367	24.30615784908933
40	25.27091460771565	24.68573905504985	28.738621586475944	21.30472475075856
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	1.0
14	2.0
15	3.0
16	10.0
17	17.0
18	17.0
19	16.0
20	15.0
21	16.5
22	18.0
23	18.0
24	24.0
25	30.0
26	30.5
27	31.0
28	31.0
29	33.5
30	36.0
31	43.0
32	50.0
33	50.0
34	66.0
35	82.0
36	101.0
37	120.0
38	120.0
39	135.5
40	151.0
41	209.0
42	267.0
43	267.0
44	295.5
45	324.0
46	328.5
47	333.0
48	333.0
49	329.0
50	325.0
51	384.5
52	444.0
53	444.0
54	495.5
55	547.0
56	505.5
57	464.0
58	464.0
59	478.0
60	492.0
61	487.0
62	482.0
63	482.0
64	346.0
65	210.0
66	151.0
67	92.0
68	92.0
69	69.0
70	46.0
71	33.0
72	20.0
73	20.0
74	14.0
75	8.0
76	5.0
77	2.0
78	2.0
79	1.5
80	1.0
81	1.5
82	2.0
83	2.0
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.06501950585175553
8	0.10836584308625921
9	0.13003901170351106
10	0.06501950585175553
11	0.0
12	0.043346337234503686
13	0.21673168617251842
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.10836584308625921
20	0.0
21	0.0
22	0.0
23	0.021673168617251843
24	0.0
25	0.10836584308625921
26	0.28175119202427396
27	0.3684438664932813
28	0.021673168617251843
29	0.6068487212830517
30	0.021673168617251843
31	0.043346337234503686
32	0.43346337234503685
33	0.0
34	0.0
35	0.0
36	0.06501950585175553
37	0.0
38	0.0
39	0.043346337234503686
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	4614.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.91200693541396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.2661356770118	72.01993931512787
2	5.877506179620983	9.276116168183789
3	1.2084592145015105	2.860858257477243
4	0.6316945893985169	1.9939315127871695
5	0.137324910738808	0.541829215431296
6	0.19225487503433122	0.9102730819245773
7	0.137324910738808	0.7585609016038145
8	0.10985992859104642	0.693541395752059
9	0.027464982147761604	0.1950585175552666
>10	0.3021148036253776	4.009536194191591
>50	0.10985992859104642	6.740355439965323
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	86	1.8638925010836587	No Hit
CCATCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	82	1.7771998266146511	No Hit
CCATCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	78	1.6905071521456438	No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	65	1.4087559601213697	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	40	0.8669267446900737	No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA	32	0.693541395752059	No Hit
CCATCTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA	15	0.3250975292587776	No Hit
CCATCTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT	14	0.30342436064152584	No Hit
CCATCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA	13	0.28175119202427396	No Hit
CCATCTGGGGGGGAGGTAGTGACGAAAAATAACAATACAG	13	0.28175119202427396	No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC	13	0.28175119202427396	No Hit
CCATCTGGGGGGGCTGGTGAGCTGGCTCAGTGGGTAAGAG	12	0.26007802340702213	No Hit
CCATCTGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT	12	0.26007802340702213	No Hit
CCATCTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT	11	0.23840485478977025	No Hit
CCATCTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT	10	0.21673168617251842	No Hit
CCATCTGGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAG	9	0.1950585175552666	No Hit
CCATCTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG	8	0.17338534893801474	No Hit
CCATCTGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT	8	0.17338534893801474	No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC	8	0.17338534893801474	No Hit
CCATCTGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA	8	0.17338534893801474	No Hit
CCATCTGGGGGTTTTCATAGAGCTGAATCACATTAAAAAG	7	0.15171218032076292	No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG	7	0.15171218032076292	No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAG	7	0.15171218032076292	No Hit
CCATCTGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT	7	0.15171218032076292	No Hit
CCATCTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC	7	0.15171218032076292	No Hit
CCATCTGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCA	6	0.13003901170351106	No Hit
CCATCTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT	6	0.13003901170351106	No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAG	6	0.13003901170351106	No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGA	6	0.13003901170351106	No Hit
CCATCTGGGTCATAGATCCTCTTCTGAAGGAGATTGGGCG	6	0.13003901170351106	No Hit
CCATCTGGGGCTCCATCTGGGTCACAGATCCTCTTCTGAA	6	0.13003901170351106	No Hit
CCATCTGGGGCTCCATCTGGGTCACAGATCCTCTTCTGAG	6	0.13003901170351106	No Hit
CCATCTGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG	5	0.10836584308625921	No Hit
CCATCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATT	5	0.10836584308625921	No Hit
CCATCTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT	5	0.10836584308625921	No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC	5	0.10836584308625921	No Hit
CCATCTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.10836584308625921	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.021673168617251843	0.0	0.0	0.0	0.0
23	0.021673168617251843	0.0	0.0	0.0	0.0
24	0.021673168617251843	0.0	0.0	0.0	0.0
25	0.021673168617251843	0.0	0.0	0.0	0.0
26	0.021673168617251843	0.0	0.0	0.0	0.0
27	0.021673168617251843	0.0	0.0	0.0	0.0
28	0.021673168617251843	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAAGGA	20	0.0024037324	34.296703	25
GAAGGAG	20	0.0024037324	34.296703	26
CTGGGGT	35	8.260158E-7	33.923916	5
CTGGGGC	30	1.207467E-5	33.923916	5
CTGGGGA	40	5.6144927E-8	33.923916	5
CTGGGCA	20	0.0025391704	33.923916	5
CTGGGAA	20	0.0025391704	33.923916	5
TGGGGGG	80	0.0	33.923916	6
TGGGGGA	35	8.260158E-7	33.923916	6
TGGGGAG	25	1.7552891E-4	33.923916	6
TCTGGGG	235	0.0	33.923912	4
TCTGGGC	45	3.788955E-9	33.923912	4
CTGGGGG	135	0.0	32.667473	5
CCATCTG	485	0.0	32.17526	1
ATCTGGG	470	0.0	32.11945	3
CATCTGG	485	0.0	31.825527	2
TCTTCTG	65	1.8189894E-12	31.658495	20
CTTCTGA	65	1.8189894E-12	31.658495	21
TCTGGGA	65	3.6379788E-12	31.314379	4
TCCTCTT	60	4.7293724E-11	31.096922	17
>>END_MODULE