FastQCFastQC Report
Fri 17 Jun 2016
SRR1525878_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525878_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27712
Sequences flagged as poor quality0
Sequence length56
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1750.6314953810623557No Hit
CGTGGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC920.331986143187067No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC720.25981524249422633No Hit
CGTGGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT590.2129041570438799No Hit
CGTGGCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT490.17681870669745958No Hit
CGTGGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC370.1335161662817552No Hit
CGTGGCGGGGCCCACTGTGAAACCACTGGGTTCTGGGTCCCAGTGGCTGAATCAGG370.1335161662817552No Hit
CGTGGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC350.12629907621247113No Hit
CGTGGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC350.12629907621247113No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC350.12629907621247113No Hit
CGTGGCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT310.11186489607390301No Hit
CGTGGCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA280.10103926096997691No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAC150.00809595250.38321341
TATCATT150.00809595250.38321338
TATGCTT309.709238E-750.2914435
AAGAGCC203.9961154E-450.2914435
ATATCAT150.00815466450.2914437
ATATGCT402.4338078E-950.20000534
TATACTA204.0687266E-450.10889444
TTATACT204.0687266E-450.10889443
ATTATAC204.0687266E-450.10889442
CACAATT150.00827303950.1088942
CAATTAT150.00827303950.1088944
ATCCTAG204.105423E-450.01811614
TGGTTGA402.5138434E-950.01811614
TCCTAGC204.105423E-450.01811615
AATTATC204.105423E-450.01811645
TATCTCA150.00833270250.01811648
TATTATA204.105423E-450.01811649
ACTATTA204.105423E-450.01811647
ACTAATC204.142382E-449.9276710
CATGATT150.00839268749.9276732