FastQCFastQC Report
Fri 17 Jun 2016
SRR1525875_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525875_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111.0242085661080074No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.74487895716946No Hit
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.5586592178770949No Hit
CGTGGCGGGGTGTTCTTTATTAATATCCTAACACTCCTCG30.27932960893854747No Hit
CGTGGCGGGGGAACAAAAAAAAAAAAAAAAAAAAAAAAAA30.27932960893854747No Hit
CGTGGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.27932960893854747No Hit
CGTGGCGGGAGTCGAATTTTTTTTTTTTTTTTTTTTTTTT30.27932960893854747No Hit
CGTGGCGGGAGCAGGATTTGTTCACTGATTCCCATTATTT30.27932960893854747No Hit
CGTGGCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.186219739292365No Hit
CGTGGCGGGACGAATTCAAAGGAAAACATGGGGTGTTTAC20.186219739292365No Hit
CGTGGCGGCACTGGAAAAAAAAAAAAAAAAAAAAAAAAAA20.186219739292365No Hit
CGTGGCGGGAAGCAGTGGTATCAAACCAAAAAAAAAAAAA20.186219739292365No Hit
CGTGGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT20.186219739292365No Hit
CGTGGCGGGAAAACAAAAAAAAAAAAAAAAAAAAAAAAAA20.186219739292365No Hit
CGTGGCGGGGGGGAGGCTTTCCCTCAGCGGAAAGGGGCCC20.186219739292365No Hit
CGTGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.186219739292365No Hit
CGTGGCGGGGGTTAAAAAAAAAAAAAAAAAAAAAACAAAA20.186219739292365No Hit
CGTGGCGGGCCCAACACCCCCAATAAAAAAACCCAACCCC20.186219739292365No Hit
CGTGGCGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT20.186219739292365No Hit
CGTGGCGGGGGGTTTTTTTTATAAAAAAAAAAAAAAAAAA20.186219739292365No Hit
CGTGGCGGGCAAGAGGATTGTGTTTTGCAATGTAACCGTA20.186219739292365No Hit
CGTGGCGGGGGAAGAGTGAGCCTGGTCAGGTGAAAGCAGC20.186219739292365No Hit
CGTGGCGGGAAAAAAAAAAAACAAAAAAAAAAAAAAAAAA20.186219739292365No Hit
CGTGGCGGGAAATCAGTTATGGTTCCTTTGGTCGCTCGCT20.186219739292365No Hit
CGTGGCGGGGCAAAAAAAAAACAAAAAAAAAAAAAAAAAA20.186219739292365No Hit
CGTGGCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG20.186219739292365No Hit
CGTGGCGGGGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAA20.186219739292365No Hit
CGTGGCGGGTAAATGGTTGAACCCGCCACTAACATATGCT20.186219739292365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGG200.001707216534.05
GCGGGGA259.5802614E-534.05
CGTGGCG1000.034.01
GTGGCGG950.034.02
TGGCGGG950.034.03
GGCGGGG700.034.04
Produced by FastQC (version 0.11.5)