SRR1525874_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1525874_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20192
Sequences flagged as poor quality	0
Sequence length	56
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	220	1.0895404120443741	No Hit
ATCTCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	182	0.9013470681458003	No Hit
ATCTCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	133	0.658676703645008	No Hit
ATCTCTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	100	0.4952456418383518	No Hit
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	66	0.3268621236133122	No Hit
ATCTCTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	61	0.30209984152139463	No Hit
ATCTCTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	59	0.29219492868462754	No Hit
ATCTCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	37	0.18324088748019018	No Hit
ATCTCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	35	0.17333597464342312	No Hit
ATCTCTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	32	0.15847860538827258	No Hit
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	32	0.15847860538827258	No Hit
ATCTCTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	30	0.14857369255150554	No Hit
ATCTCTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	30	0.14857369255150554	No Hit
ATCTCTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	30	0.14857369255150554	No Hit
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	28	0.1386687797147385	No Hit
ATCTCTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	26	0.12876386687797148	No Hit
ATCTCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	25	0.12381141045958795	No Hit
ATCTCTGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG	23	0.11390649762282092	No Hit
ATCTCTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC	22	0.1089540412044374	No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	21	0.10400158478605387	No Hit
ATCTCTGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	21	0.10400158478605387	No Hit
ATCTCTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	21	0.10400158478605387	No Hit
ATCTCTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC	21	0.10400158478605387	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCAATC	40	2.3464963E-9	50.272503	40
TTTTTGC	20	3.977188E-4	50.272503	34
ATATGCC	20	3.977188E-4	50.272503	34
TTTGTTC	50	5.456968E-12	50.272503	36
TTGTTCA	45	1.1641532E-10	50.2725	37
CCAATCC	15	0.008134512	50.2725	41
AAGAGCC	15	0.008134512	50.2725	35
GTTCAAT	45	1.1641532E-10	50.2725	39
TCAATCC	45	1.1641532E-10	50.2725	41
AGAGCCC	15	0.008134512	50.2725	36
TGGGCCG	15	0.008134512	50.2725	35
GCCGATT	15	0.008134512	50.2725	38
CCGATTG	15	0.008134512	50.2725	39
TCCGCCC	65	0.0	50.147137	45
CGCCCAA	60	0.0	50.147133	47
ATCCGCC	60	0.0	50.147133	44
TGGGGTC	50	5.456968E-12	49.898266	6
TGGGGGA	95	0.0	49.898266	6
TGGGTGT	35	5.0302333E-8	49.898266	6
TGGGATG	35	5.0302333E-8	49.898266	6

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content