##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525874_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20192
Sequences flagged as poor quality	0
Sequence length	56
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.80680467511886	39.0	37.0	40.0	33.0	40.0
2	36.13302297939778	39.0	38.0	40.0	29.0	40.0
3	35.82126584786054	39.0	36.0	40.0	28.0	40.0
4	36.083894611727416	39.0	38.0	40.0	29.0	40.0
5	35.86539223454834	39.0	37.0	40.0	29.0	40.0
6	35.82493066561014	39.0	36.0	40.0	29.0	40.0
7	36.29254160063392	39.0	38.0	40.0	31.0	40.0
8	36.14718700475436	39.0	37.0	40.0	30.0	40.0
9	36.179179873217116	39.0	38.0	40.0	30.0	40.0
10	35.49158082408875	39.0	36.0	40.0	27.0	40.0
11	35.030011885895405	38.0	35.0	40.0	27.0	40.0
12	33.916897781299525	38.0	33.0	40.0	21.0	40.0
13	33.3140352614897	38.0	32.0	39.0	18.0	40.0
14	34.02931854199683	38.0	33.0	40.0	22.0	40.0
15	32.84810816164818	38.0	31.0	39.0	15.0	40.0
16	32.33706418383518	37.0	30.0	39.0	17.0	40.0
17	31.36831418383518	36.0	28.0	39.0	13.0	40.0
18	30.7104793977813	35.0	26.0	39.0	12.0	40.0
19	31.96850237717908	36.0	29.0	39.0	16.0	40.0
20	32.43918383518225	37.0	30.0	39.0	18.0	40.0
21	32.6378763866878	38.0	30.0	39.0	17.0	40.0
22	31.854447305863708	37.0	29.0	39.0	14.0	40.0
23	32.58755942947702	38.0	30.0	39.0	17.0	40.0
24	31.985736925515056	37.0	29.0	39.0	14.0	40.0
25	32.37460380348653	37.0	30.0	39.0	17.0	40.0
26	32.89565174326466	38.0	32.0	39.0	17.0	40.0
27	33.03174524564184	38.0	32.0	39.0	18.0	40.0
28	32.975931061806655	38.0	32.0	39.0	18.0	40.0
29	32.570077258320126	38.0	31.0	39.0	16.0	40.0
30	32.34904912836767	37.0	31.0	39.0	15.0	40.0
31	32.99554278922346	38.0	33.0	39.0	16.0	40.0
32	32.84479001584786	38.0	32.0	39.0	16.0	40.0
33	32.68066561014263	37.0	32.0	39.0	14.0	40.0
34	32.55026743264659	37.0	32.0	39.0	12.0	40.0
35	32.40724049128368	37.0	32.0	39.0	10.0	40.0
36	31.52040412044374	36.0	30.0	39.0	4.0	40.0
37	31.26371830427892	36.0	30.0	38.0	4.0	40.0
38	30.65585380348653	35.0	29.0	38.0	4.0	40.0
39	29.76926505546751	35.0	27.0	38.0	4.0	40.0
40	27.509508716323296	33.0	18.0	38.0	4.0	40.0
41	25.40109944532488	31.0	12.0	37.0	4.0	39.0
42	26.369403724247228	31.0	17.0	37.0	4.0	39.0
43	26.30873613312203	32.0	17.0	37.0	4.0	39.0
44	25.445869651347067	31.0	12.0	37.0	4.0	39.0
45	26.4243264659271	32.0	18.0	37.0	4.0	39.0
46	24.342115689381934	29.0	10.0	36.0	4.0	39.0
47	23.413133914421554	28.0	6.0	35.0	4.0	39.0
48	23.994007527733757	28.0	4.0	35.0	4.0	39.0
49	22.978902535657685	27.0	4.0	35.0	4.0	39.0
50	23.612767432646592	28.0	4.0	35.0	4.0	39.0
51	23.8177000792393	29.0	4.0	36.0	4.0	39.0
52	23.63317155309033	29.0	4.0	36.0	4.0	39.0
53	23.42199881141046	29.0	4.0	36.0	4.0	39.0
54	23.10192155309033	29.0	4.0	35.0	4.0	39.0
55	22.918730190174326	29.0	4.0	35.0	4.0	39.0
56	22.517581220285262	28.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	2.6648750303226407
1	2	4.011893126781878
1	3	4.0833363401705896
1	4	4.204333663501899
1	5	4.446450072645952
1	6	4.5700475345499
1	7	3.8153999764414834
1	8	3.9722597221344955
1	9	3.9474699699300473
1	10	4.790025672475544
1	11	1.2935322399009692
1	12	-4.735699980217291
1	13	-18.577454220902943
1	14	-4.261376142550965
1	15	1.0823745305081047
1	16	-0.012706533911511997
1	17	-5.79129039232064
1	18	-3.6452269999841818
1	19	1.3138684556331093
1	20	0.8446286125826532
1	21	7.084563109607494
1	22	7.9241566652254
1	23	7.421326764098538
1	24	7.262560275748214
1	25	7.30551145880148
1	26	7.44570432113678
1	27	6.368061346442197
1	28	6.757740713543242
1	29	6.6449670183774465
1	30	7.746904015630758
1	31	5.706239404828398
1	32	5.235127779874222
1	33	5.267122041559613
1	34	6.567700103329017
1	35	7.183047706535497
1	36	5.617514616579388
1	37	6.17262765649761
1	38	6.2330927655249155
1	39	7.933610450695721
1	40	-2.181821276801138
1	41	5.988771505183468
1	42	4.5090144105626315
1	43	-3.328701277745008
1	44	-11.532805013920148
1	45	4.484874644207196
1	46	-1.9285968967592773
1	47	-6.892294356531664
1	48	-0.31441582542096924
1	49	-10.545573558133249
1	50	2.188507488574224
1	51	9.160874644309391
1	52	8.411862853938498
1	53	9.24592630640526
1	54	9.24477218241692
1	55	9.212861703386647
1	56	12.833601540034753
2	1	3.3135929790405854
2	2	4.165739280628031
2	3	4.206413263247512
2	4	4.109461868630106
2	5	3.8464500726459505
2	6	4.50338086788323
2	7	3.6871948482363592
2	8	3.6055930554678284
2	9	4.252598175058253
2	10	4.215666698116571
2	11	4.047378393747124
2	12	0.3848128402955311
2	13	2.81741757396885
2	14	5.197598216423394
2	15	5.602887351020925
2	16	6.315498594293615
2	17	7.806145505115257
2	18	5.257337102579921
2	19	6.480535122299774
2	20	5.088218356172394
2	21	7.5512297762741625
2	22	8.206207947276678
2	23	7.659788302560081
2	24	7.55743207062001
2	25	6.746537099827119
2	26	5.0354479108803645
2	27	5.58857416695502
2	28	5.685945841748371
2	29	5.850095223505651
2	30	5.644339913066656
2	31	3.765213763802759
2	32	5.591538036284472
2	33	4.67994255438013
2	34	4.86513600076492
2	35	4.877919501407291
2	36	4.861104360169133
2	37	5.103396887266847
2	38	6.371554303986457
2	39	6.751559168644437
2	40	4.72587103089117
2	41	9.452874069286032
2	42	8.998758000306225
2	43	7.889247440203707
2	44	-2.2404973216124553
2	45	8.866925926258478
2	46	5.748326180163801
2	47	-0.24870461294192125
2	48	5.206096995091851
2	49	-0.948137660697352
2	50	6.557738257804996
2	51	7.860874644309391
2	52	8.945196187271833
2	53	8.958746819225773
2	54	9.655028592673329
2	55	7.407733498258441
2	56	8.646422052855264
3	1	2.2469263123739225
3	2	3.5990726139613614
3	3	4.106413263247511
3	4	3.5761285352967747
3	5	4.046450072645953
3	6	3.770047534549903
3	7	2.1538615149030207
3	8	3.13892638880116
3	9	3.319264841724923
3	10	4.249000031449903
3	11	3.847378393747121
3	12	4.78481284029553
3	13	4.750750907302187
3	14	5.03093154975673
3	15	0.702887351020923
3	16	7.448831927626948
3	17	7.139478838448593
3	18	7.824003769246591
3	19	6.647201788966445
3	20	4.821551689505728
3	21	6.0512297762741625
3	22	8.239541280610016
3	23	6.793121635893414
3	24	7.05743207062001
3	25	7.613203766493786
3	26	4.43544791088037
3	27	5.655240833621683
3	28	4.419279175081705
3	29	6.183428556838987
3	30	6.111006579733324
3	31	5.565213763802756
3	32	3.724871369617812
3	33	5.17994255438013
3	34	5.29846933409825
3	35	5.244586168073958
3	36	5.6944376935024685
3	37	6.503396887266845
3	38	6.638220970653123
3	39	5.1182258353111045
3	40	7.825871030891172
3	41	10.519540735952695
3	42	8.565424666972888
3	43	8.322580773537041
3	44	9.02616934505421
3	45	8.46692592625848
3	46	10.58165951349713
3	47	12.451295387058078
3	48	10.139430328425181
3	49	9.718529005969316
3	50	9.92440492447166
3	51	9.760874644309393
3	52	9.478529520605164
3	53	9.092080152559106
3	54	9.25502859267333
3	55	8.274400164925108
3	56	6.4797553861886
4	1	3.462077827525434
4	2	4.741496856385609
4	3	4.779140535974783
4	4	4.667037626205861
4	5	4.779783405979288
4	6	5.085199049701416
4	7	4.308406969448477
4	8	4.305593055467831
4	9	4.440476962937041
4	10	5.073242455692331
4	11	5.568590514959247
4	12	6.327237082719769
4	13	6.535599392150669
4	14	5.885477004302182
4	15	6.8271297752633515
4	16	8.979134957929979
4	17	9.697054596024348
4	18	10.199761345004166
4	19	9.16235330411796
4	20	8.542763810717855
4	21	8.523957049001432
4	22	8.933480674549404
4	23	7.914333757105531
4	24	8.345310858498799
4	25	8.09805225134227
4	26	6.838478213910669
4	27	7.006755985136834
4	28	6.082915538718069
4	29	6.244034617445049
4	30	7.962521731248476
4	31	6.398547097136092
4	32	6.627901672648111
4	33	6.825397099834671
4	34	6.707560243189157
4	35	7.23549525898305
4	36	7.467164966229742
4	37	7.888245372115328
4	38	8.907917940350089
4	39	7.7424682595535295
4	40	-0.7377653327451945
4	41	10.174086190498151
4	42	10.962394363942586
4	43	11.025611076567344
4	44	12.083745102629965
4	45	9.569956229288781
4	46	13.04226557410319
4	47	14.987659023421717
4	48	14.233369722364575
4	49	13.306407793848106
4	50	13.79107159113833
4	51	13.009359492794243
4	52	12.83610527818092
4	53	13.773898334377286
4	54	13.821695259339997
4	55	14.053188043712982
4	56	14.649452355885568
5	1	3.1135929790405896
5	2	4.299072613961364
5	3	4.406413263247508
5	4	4.27612853529677
5	5	4.679783405979286
5	6	4.63671420121657
5	7	3.9205281815696935
5	8	4.005593055467827
5	9	4.019264841724919
5	10	4.11566669811657
5	11	5.347378393747121
5	12	5.651479506962197
5	13	7.084084240635523
5	14	6.2309315497567255
5	15	6.669554017687588
5	16	8.282165260960284
5	17	9.206145505115256
5	18	10.324003769246591
5	19	8.88053512229978
5	20	6.721551689505727
5	21	7.317896442940828
5	22	8.572874613943345
5	23	7.759788302560075
5	24	8.55743207062001
5	25	7.746537099827119
5	26	6.835447910880369
5	27	6.95524083362168
5	28	6.119279175081701
5	29	7.116761890172317
5	30	7.544339913066661
5	31	5.265213763802759
5	32	6.758204702951144
5	33	6.746609221046793
5	34	6.29846933409825
5	35	6.744586168073958
5	36	7.2611043601691385
5	37	7.003396887266845
5	38	6.4048876373197885
5	39	7.984892501977772
5	40	9.325871030891172
5	41	10.619540735952697
5	42	10.69875800030622
5	43	10.955914106870374
5	44	11.426169345054209
5	45	9.933592592925148
5	46	10.448326180163797
5	47	11.817962053724745
5	48	10.272763661758514
5	49	11.985195672635978
5	50	12.124404924471659
5	51	11.960874644309389
5	52	12.311862853938496
5	53	11.892080152559103
5	54	11.255028592673327
5	55	11.674400164925107
5	56	8.513088719521932
6	1	3.5469263123739196
6	2	4.6324059472947
6	3	4.839746596580845
6	4	4.376128535296772
6	5	4.746450072645949
6	6	5.0700475345499
6	7	4.153861514903021
6	8	4.172259722134498
6	9	4.319264841724923
6	10	4.882333364783236
6	11	5.714045060413788
6	12	6.451479506962194
6	13	7.050750907302184
6	14	6.264264883090064
6	15	6.969554017687592
6	16	8.382165260960278
6	17	9.772812171781926
6	18	10.024003769246587
6	19	8.780535122299778
6	20	7.954885022839061
6	21	8.451229776274161
6	22	8.539541280610013
6	23	7.959788302560078
6	24	7.690765403953343
6	25	8.446537099827122
6	26	6.30211457754703
6	27	6.355240833621686
6	28	6.119279175081701
6	29	7.116761890172317
6	30	6.077673246399993
6	31	5.7318804304694275
6	32	5.858204702951145
6	33	5.279942554380124
6	34	5.665136000764917
6	35	5.811252834740621
6	36	8.127771026835799
6	37	7.370063553933512
6	38	8.238220970653124
6	39	8.351559168644439
6	40	5.22587103089117
6	41	13.619540735952697
6	42	11.532091333639556
6	43	12.389247440203711
6	44	11.392836011720878
6	45	11.500259259591811
6	46	13.21499284683047
6	47	13.584628720391411
6	48	14.006096995091848
6	49	14.251862339302651
6	50	14.591071591138327
6	51	13.827541310976056
6	52	13.1118628539385
6	53	13.25874681922577
6	54	13.52169525934
6	55	13.141066831591775
6	56	13.21308871952193
7	1	2.7318725489330618
7	2	3.810900570950615
7	3	4.441897134215253
7	4	4.081504879382791
7	5	4.349675879097568
7	6	4.4410152764853805
7	7	3.757087321354639
7	8	3.9077435931022393
7	9	3.9859315083915874
7	10	4.433946268009045
7	11	4.382862264714866
7	12	5.256855851048215
7	13	6.932471337409712
7	14	5.334157356208344
7	15	6.736220684354258
7	16	8.303670637304364
7	17	8.745930451351814
7	18	9.559487640214332
7	19	8.442900713697629
7	20	7.453809754021858
7	21	7.261982464446206
7	22	8.410509022545494
7	23	7.233981850947174
7	24	7.159582608254418
7	25	6.613203766493786
7	26	6.481684470020149
7	27	6.199326855127062
7	28	5.46121465895267
7	29	6.764073718129307
7	30	6.251866794787087
7	31	5.656611613265124
7	32	6.077559541660822
7	33	6.2584371780360435
7	34	5.959759656678898
7	35	5.563941006783637
7	36	6.517018338663757
7	37	6.435654951782972
7	38	7.594134949147744
7	39	8.092419383698203
7	40	6.948451676052457
7	41	10.493734284339794
7	42	10.448220365897619
7	43	11.184946364934895
7	44	11.949825259032703
7	45	10.76154958217246
7	46	12.680584244679928
7	47	14.265273881681733
7	48	13.387817425199376
7	49	12.733582769410173
7	50	14.070641483611443
7	51	12.547971418502943
7	52	12.218314466841722
7	53	11.979176926752658
7	54	12.499114614178708
7	55	12.238916293957363
7	56	10.099110224898276
8	1	3.346926312373924
8	2	4.165739280628031
8	3	4.506413263247509
8	4	4.042795201963436
8	5	3.6131167393126162
8	6	4.270047534549903
8	7	3.887194848236355
8	8	3.2722597221344927
8	9	3.752598175058253
8	10	3.849000031449904
8	11	4.74737839374712
8	12	6.084812840295527
8	13	6.550750907302184
8	14	5.96426488309006
8	15	6.2695540176875895
8	16	8.715498594293614
8	17	9.772812171781926
8	18	9.824003769246591
8	19	7.280535122299778
8	20	7.321551689505728
8	21	7.751229776274158
8	22	8.572874613943345
8	23	7.659788302560081
8	24	8.124098737286673
8	25	6.046537099827116
8	26	5.335447910880369
8	27	4.388574166955017
8	28	5.852612508415035
8	29	5.550095223505654
8	30	6.177673246399994
8	31	3.698547097136089
8	32	3.591538036284472
8	33	4.279942554380124
8	34	4.065136000764916
8	35	3.6112528347406254
8	36	5.594437693502467
8	37	5.070063553933508
8	38	5.304887637319787
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115	24	6.779654292842231
115	25	5.946537099827122
115	26	5.5354479108803645
115	27	5.355240833621686
115	28	5.663723619526145
115	29	4.794539667950101
115	30	5.5332288019555484
115	31	3.398547097136092
115	32	4.991538036284478
115	33	3.5577203321579027
115	34	5.076247111876029
115	35	5.422363945851735
115	36	6.805548804613583
115	37	7.181174665044622
115	38	6.493776526208677
115	39	8.651559168644443
115	40	10.181426586446726
115	41	9.163985180397141
115	42	7.987646889195112
115	43	8.833691884648154
115	44	0.32616934505421113
115	45	7.600259259591812
115	46	4.037215069052689
115	47	-5.426482390719698
115	48	5.172763661758516
115	49	-1.2036932162529084
115	50	4.457738257804994
115	51	6.2164301998649485
115	52	3.411862853938498
115	53	4.647635708114663
115	54	4.9883619260066645
115	55	6.007733498258439
115	56	6.068644275077489
116	1	3.2385929790405896
116	2	4.124072613961367
116	3	4.548079929914174
116	4	3.867795201963439
116	5	3.7381167393126162
116	6	4.278380867883236
116	7	3.4371948482363592
116	8	3.4722597221344955
116	9	3.4025981750582517
116	10	4.149000031449901
116	11	4.1973783937471225
116	12	4.4181461736288625
116	13	5.842417573968856
116	14	4.555931549756728
116	15	-1.6804459823124098
116	16	3.948831927626948
116	17	6.397812171781926
116	18	5.332337102579924
116	19	6.480535122299774
116	20	5.588218356172394
116	21	6.29289644294083
116	22	8.497874613943349
116	23	6.126454969226742
116	24	7.974098737286674
116	25	6.9048704331604505
116	26	5.4104479108803645
116	27	5.27190750028835
116	28	5.3859458417483665
116	29	6.475095223505651
116	30	5.727673246399991
116	31	5.3568804304694275
116	32	5.783204702951142
116	33	6.029942554380124
116	34	5.67346933409825
116	35	5.352919501407293
116	36	6.3194376935024685
116	37	7.86173022060018
116	38	7.354887637319791
116	39	9.151559168644443
116	40	8.584204364224505
116	41	11.052874069286034
116	42	6.848758000306223
116	43	4.597580773537043
116	44	4.826169345054211
116	45	8.016925926258477
116	46	4.439992846830467
116	47	-2.3570379462752555
116	48	2.214430328425184
116	49	0.9768623393026488
116	50	3.707738257804994
116	51	6.452541310976059
116	52	6.411862853938498
116	53	7.342080152559106
116	54	7.405028592673329
116	55	6.632733498258439
116	56	8.3047553861886
117	1	1.753943856233569
117	2	2.5867919122069765
117	3	2.9274658948264545
117	4	2.716479412489754
117	5	2.867502704224897
117	6	3.201626481918318
117	7	2.485440462271441
117	8	2.5512070905555504
117	9	2.4596157189179024
117	10	2.1928596805727096
117	11	1.0263257621681774
117	12	-6.5292222474237676
117	13	-8.773810496206586
117	14	-1.0427526607695867
117	15	-10.947989841961533
117	16	-8.156431230267788
117	17	0.8342156805538536
117	18	-1.1128383360165692
117	19	0.5243947714225818
117	20	-0.5258167315469002
117	21	4.7950894253969665
117	22	5.750067596399486
117	23	5.249261986770605
117	24	5.645151368865619
117	25	5.350045871756944
117	26	3.92141282316107
117	27	4.153486447656768
117	28	3.40348970139749
117	29	4.183428556838987
117	30	5.161883772715779
117	31	3.4511786760834617
117	32	4.079257334530087
117	33	3.66590746666083
117	34	3.956364070940353
117	35	3.618270378600272
117	36	5.983911377712996
117	37	5.140238992530001
117	38	7.061027988196983
117	39	7.791910045837422
117	40	9.889028925628011
117	41	5.877435472794804
117	42	3.45840712311324
117	43	-3.4704016826033097
117	44	-3.8141815321387718
117	45	4.968680312223391
117	46	1.069378811742748
117	47	-0.5258975953980602
117	48	1.365746117898869
117	49	-5.814804327364019
117	50	1.1945803630681517
117	51	4.58192727588834
117	52	5.464494432885868
117	53	5.855238047295948
117	54	5.769063680392627
117	55	5.700715954398792
117	56	8.785018544083336
118	1	1.8135929790405854
118	2	2.6990726139613628
118	3	3.039746596580841
118	4	2.5094618686301047
118	5	2.8464500726459505
118	6	3.0367142012165687
118	7	2.387194848236355
118	8	2.505593055467827
118	9	2.319264841724923
118	10	3.3823333647832357
118	11	0.5140450604137925
118	12	-5.848520493037803
118	13	-3.2159157593644814
118	14	-9.902401783576604
118	15	-9.397112648979075
118	16	-13.584501405706387
118	17	0.20614550511525565
118	18	-7.309329564086745
118	19	-0.2194648777002257
118	20	-0.3117816438276044
118	21	4.717896442940827
118	22	5.939541280610012
118	23	5.393121635893408
118	24	5.890765403953338
118	25	5.146537099827118
118	26	5.80211457754703
118	27	5.721907500288353
118	28	5.4526125084150365
118	29	5.916761890172321
118	30	6.2776732463999885
118	31	4.065213763802756
118	32	5.124871369617811
118	33	5.479942554380127
118	34	5.86513600076492
118	35	5.811252834740621
118	36	7.7277710268358
118	37	7.536730220600177
118	38	8.338220970653126
118	39	9.118225835311105
118	40	11.092537697557837
118	41	11.719540735952698
118	42	10.19875800030622
118	43	3.6559141068703767
118	44	5.192836011720878
118	45	8.733592592925145
118	46	5.5149928468304665
118	47	5.617962053724746
118	48	4.506096995091852
118	49	3.2518623393026473
118	50	6.924404924471659
118	51	11.427541310976057
118	52	12.345196187271828
118	53	10.458746819225773
118	54	12.221695259339995
118	55	11.974400164925104
118	56	13.6797553861886
119	1	1.8802596457072553
119	2	3.2157392806280285
119	3	3.456413263247512
119	4	2.8427952019634404
119	5	3.2964500726459534
119	6	3.1200475345498973
119	7	2.9038615149030207
119	8	3.0222597221344927
119	9	2.885931508391586
119	10	3.732333364783237
119	11	2.297378393747124
119	12	-5.881853826371138
119	13	-0.9159157593644807
119	14	-3.6190684502432724
119	15	-8.563779315645743
119	16	-12.801168072373052
119	17	1.18947883844859
119	18	-3.8759962307534117
119	19	0.5638684556331093
119	20	-0.27844831049426944
119	21	5.184563109607495
119	22	4.539541280610013
119	23	4.659788302560081
119	24	3.3240987372866755
119	25	4.413203766493783
119	26	5.8687812442137
119	27	4.621907500288351
119	28	5.069279175081704
119	29	5.333428556838985
119	30	5.677673246399994
119	31	4.54854709713609
119	32	5.058204702951141
119	33	4.063275887713463
119	34	5.54846933409825
119	35	5.644586168073957
119	36	5.994437693502466
119	37	6.103396887266847
119	38	6.521554303986456
119	39	7.318225835311107
119	40	8.625871030891169
119	41	6.219540735952698
119	42	7.732091333639556
119	43	-1.7440858931296255
119	44	0.05950267838754186
119	45	7.6502592595918095
119	46	0.04832618016379797
119	47	2.1846287203914123
119	48	-4.5605696715748145
119	49	-2.2648043273640184
119	50	0.7077382578049942
119	51	6.06087464430939
119	52	6.711862853938499
119	53	6.892080152559107
119	54	5.521695259339996
119	55	6.2244001649251075
119	56	5.079755386188598
120	1	1.9802596457072568
120	2	3.81573928062803
120	3	3.5564132632475065
120	4	3.742795201963439
120	5	3.946450072645952
120	6	4.020047534549903
120	7	3.503861514903022
120	8	3.622259722134494
120	9	3.2359315083915874
120	10	3.6323333647832357
120	11	-0.35262160625287464
120	12	-3.881853826371138
120	13	-6.5159157593644785
120	14	-6.669068450243273
120	15	0.736220684354258
120	16	-9.70116807237305
120	17	1.0394788384485913
120	18	-3.825996230753411
120	19	0.26386845563310857
120	20	-0.07844831049427015
120	21	4.434563109607495
120	22	6.489541280610016
120	23	5.159788302560081
120	24	5.874098737286673
120	25	5.113203766493786
120	26	6.3687812442137
120	27	6.171907500288349
120	28	6.769279175081699
120	29	5.6334285568389895
120	30	6.5276732463999885
120	31	5.898547097136092
120	32	3.858204702951145
120	33	4.2632758877134584
120	34	5.29846933409825
120	35	5.84458616807396
120	36	6.644437693502471
120	37	6.703396887266841
120	38	7.421554303986454
120	39	8.618225835311105
120	40	8.875871030891169
120	41	-6.530459264047302
120	42	-2.9679086663604437
120	43	-11.144085893129624
120	44	-13.140497321612457
120	45	-6.199740740408188
120	46	-8.9516738198362
120	47	-10.765371279608587
120	48	-7.810569671574816
120	49	-14.164804327364019
120	50	-5.742261742195005
120	51	-1.68912535569061
120	52	-1.2881371460615014
120	53	-0.9579198474408948
120	54	-1.3783047406600026
120	55	-0.9755998350748918
120	56	1.3797553861885987
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	859.0
5	9.0
6	15.0
7	90.0
8	141.0
9	92.0
10	81.0
11	59.0
12	61.0
13	51.0
14	73.0
15	61.0
16	99.0
17	133.0
18	142.0
19	158.0
20	208.0
21	291.0
22	334.0
23	398.0
24	466.0
25	526.0
26	593.0
27	662.0
28	689.0
29	729.0
30	794.0
31	849.0
32	1056.0
33	1172.0
34	1329.0
35	1366.0
36	1486.0
37	1598.0
38	1728.0
39	1793.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.23236925515056	0.6240095087163232	0.09904912836767037	0.04457210776545167
8	99.5790412044374	0.22286053882725831	0.11885895404120445	0.07923930269413629
9	98.80150554675119	0.4754358161648178	0.39619651347068147	0.3268621236133122
10	53.59053090332805	19.586965134706816	16.744255150554675	10.07824881141046
11	36.72741679873217	24.29179873217116	17.843700475435817	21.137083993660855
12	31.131141045958792	19.066957210776543	20.38431061806656	29.4175911251981
13	23.826267828843108	15.506141045958795	23.390451664025356	37.277139461172744
14	23.177496038034864	26.069730586370838	26.460974643423135	24.29179873217116
15	30.266825965750698	20.89804858622063	23.043608124253286	25.791517323775388
16	29.875148632580263	24.796868806975823	23.414585810543002	21.91339674990091
17	28.16524668119675	19.03606102635229	31.736675252625325	21.06201703982564
18	23.806458003169574	17.93284469096672	28.020998415213942	30.239698890649763
19	27.816308332507678	20.81145348261171	20.563757059348063	30.80848112553255
20	26.617741855506523	22.71532701938811	28.670600485942384	21.996330639162988
21	24.980190174326466	20.79536450079239	25.92610935023772	28.298335974643425
22	22.7169175911252	19.141244057052297	31.289619651347067	26.852218700475433
23	22.20186212361331	21.66699683042789	28.838153724247228	27.29298732171157
24	21.74666864813989	21.93986228761084	25.536236191608463	30.77723287264081
25	26.59469096671949	23.504358161648177	26.698692551505548	23.202258320126784
26	31.55835149593818	22.191400832177532	21.28987517337032	24.96037249851397
27	25.090422632908883	25.917851657335383	20.740226923648617	28.25149878610712
28	27.99128367670364	26.13906497622821	22.563391442155307	23.30625990491284
29	32.69860160666468	24.090052563721116	18.714668253495983	24.49667757611822
30	28.519050686221075	21.116781449734926	27.384432443145222	22.979735420898777
31	28.83253256723959	26.60854920996582	22.338897419386793	22.2200208034078
32	32.64820959833589	24.85760982616017	21.43529295230548	21.058887623198455
33	29.245563596708635	24.556359670863486	22.29602458610092	23.902052146326955
34	24.87974212744855	23.367220431440614	29.957847756012896	21.79518968509794
35	24.032929974211466	23.497321959928584	32.57290220194406	19.896845863915892
36	26.776335348330193	25.49796848677039	26.201565751659896	21.52413041323952
37	28.286635259112323	21.725762459707415	28.29159434664022	21.696007934540045
38	30.27491067884081	24.220921000396984	25.277888050813814	20.226280269948393
39	28.28738595795319	22.991868306227687	21.008528361761204	27.712217374057914
40	22.941734484996754	19.067352339108293	24.6143092515852	33.37660392430975
41	21.56539134783696	15.973993498374595	22.99574893723431	39.46486621655414
42	24.431536093734486	25.12660113196306	22.83288650580876	27.608976268493695
43	19.621385272781477	21.867236410613135	28.31660538606777	30.19477293053761
44	21.62485065710872	23.830147351652727	25.393269613699722	29.15173237753883
45	25.158761659059337	22.608652510418732	28.418336971621354	23.814248858900573
46	21.218748446741888	25.84124459466176	24.62846065907848	28.31154629951787
47	18.989295494149864	24.321633059497135	24.311675379636544	32.37739606671646
48	25.02229709642255	23.382221781785752	25.01238727579031	26.583093846001386
49	20.950677991357473	24.89941886455074	24.164307356082055	29.985595788009732
50	24.258358964182953	24.03512253199722	26.327016569104078	25.379501934715748
51	23.81685454635745	24.90937080995183	26.53821323931072	24.735561404379997
52	22.419434804164602	27.124442240951907	26.722855726326227	23.733267228557263
53	22.263027295285358	25.955334987593055	27.38957816377171	24.392059553349878
54	23.338132353671252	25.130318224693443	27.746611726157973	23.78493769547734
55	22.456575682382134	23.905707196029777	27.83622828784119	25.801488833746898
56	24.34001587931719	21.29813418023025	29.06907502977372	25.29277491067884
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	6.0
9	10.0
10	16.5
11	23.0
12	29.5
13	39.0
14	42.0
15	40.5
16	39.0
17	35.5
18	32.0
19	33.0
20	34.0
21	31.5
22	39.0
23	49.0
24	52.5
25	56.0
26	63.5
27	71.0
28	80.0
29	99.0
30	109.0
31	131.0
32	153.0
33	184.5
34	216.0
35	238.0
36	260.0
37	280.5
38	333.5
39	366.0
40	444.5
41	523.0
42	709.5
43	896.0
44	1228.0
45	1560.0
46	1563.0
47	1362.0
48	1158.0
49	1154.5
50	1151.0
51	1180.0
52	1209.0
53	1220.5
54	1244.0
55	1256.0
56	1281.5
57	1307.0
58	1325.5
59	1344.0
60	1253.0
61	1162.0
62	1081.0
63	929.5
64	859.0
65	716.5
66	574.0
67	528.5
68	483.0
69	414.5
70	346.0
71	318.5
72	226.5
73	162.0
74	119.5
75	77.0
76	61.0
77	45.0
78	46.0
79	33.0
80	19.0
81	13.0
82	7.0
83	4.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5150554675118859
16	0.039619651347068144
17	0.019809825673534072
18	0.0
19	0.02971473851030111
20	0.12381141045958795
21	0.0
22	0.0
23	0.0
24	0.02476228209191759
25	0.0
26	0.019809825673534072
27	0.04457210776545167
28	0.0
29	0.12876386687797148
30	0.04457210776545167
31	0.014857369255150556
32	0.004952456418383518
33	0.08914421553090333
34	0.133716323296355
35	0.1386687797147385
36	0.04952456418383518
37	0.133716323296355
38	0.19809825673534073
39	0.11885895404120445
40	0.8072503961965134
41	0.9756339144215531
42	0.2476228209191759
43	0.3268621236133122
44	0.5150554675118859
45	0.17828843106180667
46	0.3615293185419968
47	0.5299128367670365
48	0.04952456418383518
49	0.29219492868462754
50	0.16838351822503964
51	0.27238510301109353
52	0.1089540412044374
53	0.20800316957210774
54	0.24267036450079238
55	0.20800316957210774
56	0.19809825673534073
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	20192.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.66858161648177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.00451600235617	67.34845483359746
2	6.688919431899993	10.122820919175911
3	1.8456705281759276	4.189778129952456
4	0.7788467831664375	2.3573692551505547
5	0.4188755808626219	1.5847860538827259
6	0.2552523070881602	1.1588748019017432
7	0.18325806662739708	0.9706814580031695
8	0.15053341187250474	0.9112519809825673
9	0.09162903331369854	0.6240095087163232
>10	0.5366843379802343	6.666006339144216
>50	0.026179723803913868	1.4164025356576861
>100	0.0196347928529354	2.6495641838351824
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	220	1.0895404120443741	No Hit
ATCTCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	182	0.9013470681458003	No Hit
ATCTCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	133	0.658676703645008	No Hit
ATCTCTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	100	0.4952456418383518	No Hit
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	66	0.3268621236133122	No Hit
ATCTCTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	61	0.30209984152139463	No Hit
ATCTCTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	59	0.29219492868462754	No Hit
ATCTCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	37	0.18324088748019018	No Hit
ATCTCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	35	0.17333597464342312	No Hit
ATCTCTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	32	0.15847860538827258	No Hit
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	32	0.15847860538827258	No Hit
ATCTCTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	30	0.14857369255150554	No Hit
ATCTCTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	30	0.14857369255150554	No Hit
ATCTCTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	30	0.14857369255150554	No Hit
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	28	0.1386687797147385	No Hit
ATCTCTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	26	0.12876386687797148	No Hit
ATCTCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	25	0.12381141045958795	No Hit
ATCTCTGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG	23	0.11390649762282092	No Hit
ATCTCTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC	22	0.1089540412044374	No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	21	0.10400158478605387	No Hit
ATCTCTGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	21	0.10400158478605387	No Hit
ATCTCTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	21	0.10400158478605387	No Hit
ATCTCTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC	21	0.10400158478605387	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.004952456418383518	0.0	0.0	0.0	0.0
14	0.02476228209191759	0.0	0.0	0.0	0.0
15	0.029714738510301108	0.0	0.0	0.0	0.0
16	0.029714738510301108	0.0	0.0	0.0	0.0
17	0.029714738510301108	0.0	0.0	0.0	0.0
18	0.03466719492868463	0.0	0.0	0.0	0.0
19	0.03466719492868463	0.0	0.0	0.0	0.0
20	0.03466719492868463	0.0	0.0	0.0	0.0
21	0.039619651347068144	0.0	0.0	0.0	0.0
22	0.04457210776545167	0.0	0.0	0.0	0.0
23	0.04457210776545167	0.0	0.0	0.0	0.0
24	0.04457210776545167	0.0	0.0	0.0	0.0
25	0.04457210776545167	0.0	0.0	0.0	0.0
26	0.04952456418383518	0.0	0.0	0.0	0.0
27	0.04952456418383518	0.0	0.0	0.0	0.0
28	0.0544770206022187	0.0	0.0	0.0	0.0
29	0.059429477020602216	0.0	0.0	0.0	0.0
30	0.059429477020602216	0.0	0.0	0.0	0.0
31	0.07923930269413629	0.0	0.0	0.0	0.0
32	0.07923930269413629	0.0	0.0	0.0	0.0
33	0.0841917591125198	0.0	0.0	0.0	0.0
34	0.08914421553090333	0.0	0.0	0.0	0.0
35	0.09409667194928685	0.0	0.0	0.0	0.0
36	0.09409667194928685	0.0	0.0	0.0	0.0
37	0.09904912836767037	0.0	0.0	0.0	0.0
38	0.10400158478605388	0.0	0.0	0.0	0.0
39	0.10400158478605388	0.0	0.0	0.0	0.0
40	0.1089540412044374	0.0	0.0	0.0	0.0
41	0.1089540412044374	0.0	0.0	0.0	0.0
42	0.1089540412044374	0.0	0.0	0.0	0.0
43	0.11390649762282092	0.0	0.0	0.0	0.0
44	0.11390649762282092	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCAATC	40	2.3464963E-9	50.272503	40
TTTTTGC	20	3.977188E-4	50.272503	34
ATATGCC	20	3.977188E-4	50.272503	34
TTTGTTC	50	5.456968E-12	50.272503	36
TTGTTCA	45	1.1641532E-10	50.2725	37
CCAATCC	15	0.008134512	50.2725	41
AAGAGCC	15	0.008134512	50.2725	35
GTTCAAT	45	1.1641532E-10	50.2725	39
TCAATCC	45	1.1641532E-10	50.2725	41
AGAGCCC	15	0.008134512	50.2725	36
TGGGCCG	15	0.008134512	50.2725	35
GCCGATT	15	0.008134512	50.2725	38
CCGATTG	15	0.008134512	50.2725	39
TCCGCCC	65	0.0	50.147137	45
CGCCCAA	60	0.0	50.147133	47
ATCCGCC	60	0.0	50.147133	44
TGGGGTC	50	5.456968E-12	49.898266	6
TGGGGGA	95	0.0	49.898266	6
TGGGTGT	35	5.0302333E-8	49.898266	6
TGGGATG	35	5.0302333E-8	49.898266	6
>>END_MODULE