FastQCFastQC Report
Fri 17 Jun 2016
SRR1525873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG90.37052284890901604No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC60.24701523260601072No Hit
ATCTCTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT50.20584602717167558No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC50.20584602717167558No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG50.20584602717167558No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC40.16467682173734047No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG40.16467682173734047No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC40.16467682173734047No Hit
ATCTCTGGGTGTTAACTTTGGGGAAGGAAACATTGTATGT30.12350761630300536No Hit
ATCTCTGGGCTCGTCTTACTCAGCAACATCTTTTGATACA30.12350761630300536No Hit
ATCTCTGGGGATCCAGTGACAGATACCATTAGATTCAAGA30.12350761630300536No Hit
ATCTCTGGGGTGGCTTTAGGTCTGACATTGTATTTGACCT30.12350761630300536No Hit
ATCTCTGGGGAGCTGCAGGCCTAGCGGGGGACCAGGTTCG30.12350761630300536No Hit
ATCTCTGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.12350761630300536No Hit
ATCTCTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCC30.12350761630300536No Hit
ATCTCTGGGGGCTGGTGTGTAGAACTGCTCCCGGCTTTCT30.12350761630300536No Hit
ATCTCTGGGTCGCAGAGTCGATTTTTTTTTTTTTTTTTTT30.12350761630300536No Hit
ATCTCTGGGGGTTAGAGGAGAGAGCTGTGACCGGTTGTAC30.12350761630300536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGGG559.094947E-1233.8541685
CTGGGCT200.002321320833.8541685
CTGGGAA200.002321320833.8541685
TCTGGGC356.2883737E-733.8541684
TGGGGGG403.9261067E-833.8541686
CTGGGGC309.885391E-633.8541645
TCTGGGT309.885391E-633.8541644
ATCTCTG2500.032.51
TCTCTGG2450.032.4723632
TCTGGGG1100.032.3153424
CTCTGGG2450.031.7814643
TCTGGGA703.6379788E-1229.0178574
CTGGGAC250.006929682627.0833345
TTTTTTT3350.00685725135.55814733
Produced by FastQC (version 0.11.5)