FastQCFastQC Report
Fri 17 Jun 2016
SRR1525872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159
Sequences flagged as poor quality0
Sequence length40
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC21.257861635220126No Hit
ATCTCTGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT21.257861635220126No Hit
ATCTCTGGGGTGCTATCTCTGGGAGCATTACGCCGAGTCG10.628930817610063No Hit
ATCTCTGGGATTTCTTTCTTTCTAACCTTGCCCTCTGCCT10.628930817610063No Hit
ATCTCTGGGCCCTCGTATAACGCAGAGTCGACTTTTTTTT10.628930817610063No Hit
ATCTCTGGGAAATGTATACCCCCCCGCCCCCCTTTTTTTT10.628930817610063No Hit
ATCTCTGGGAAGCACTTATACAAAGAACCTCTGGGGTGTT10.628930817610063No Hit
ATCTCTGGGTCCCTCCTTTTTTTTTTTCTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGGGCTTTTTCGCTCCCCTGCTTCCCCCCCTCT10.628930817610063No Hit
ATCTCTGGGAGTGTTACGCAGAGTCCACTTTTTTTTTTTC10.628930817610063No Hit
ATCTCTGGGCTATGGTGATCATTGTGGCTTCCTTCTGGGG10.628930817610063No Hit
ATCTCTGGGATGGTTTGTTTTGTTTTTTTACTGGGTTGTG10.628930817610063No Hit
ATCTCTGGGATCATTGAAAAAAAACCAAACACAACCCCCC10.628930817610063No Hit
ATCTCTGGGGTCTGAGTGGCAACATCAGAGGCACACACTT10.628930817610063No Hit
ATCTCTGGGAACAGTGCTATCACCGCCGAGTCGCCTTTTT10.628930817610063No Hit
ATCTCTGGGTCCAGCGTCCCTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTAAACAAATTTCACTCAAAAAAACCCCACCTCCTC10.628930817610063No Hit
ATCTCTGGGAGGAAAAGGGAGAAACCACACCACCTTGAGA10.628930817610063No Hit
ATCTCTGGGAGTGCAGTGCTATCCCTGGGGTGCCCCACCC10.628930817610063No Hit
ATCTCTGGGGGAAAAAACCAAAAAAAAAAACAAAAAAAAA10.628930817610063No Hit
ATCTCTGGGAAGCAGTTCCCAACCCCGAGTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGACTCGTCATTGATCCTTTTTAAATGCTGCCA10.628930817610063No Hit
ATCTCTGGGTGTCAGTGGTCCCATCCCTTCACTGGAGCTC10.628930817610063No Hit
ATCTCTGGGACCATGTTCCACCCACCGCCGCCCTCCCTTT10.628930817610063No Hit
ATCTCTGGGGAAAACCCAAACCAAAAAAAAACCAACAAAA10.628930817610063No Hit
ATCTCTGGGGGGGCTGGGGATTTAGCTCAGTGGTAGACCC10.628930817610063No Hit
ATCTCTGGGGAATACAGTGCTTTCTCTGAAAACAAAACAC10.628930817610063No Hit
ATCTCTGGGACGCATGGTATCAACCCAAAAAAAAAAAAAA10.628930817610063No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTG10.628930817610063No Hit
ATCTCTGGGAAACAAACCTCTTTTAGCCGCAACTGTTGGA10.628930817610063No Hit
ATCTCTGGGGGGGTTGGGGCTTTAGCTCCGTGGTAGAGCC10.628930817610063No Hit
ATCTCTGGGAAGCATGTACAACCCCCCGTCGCCTTTTTTT10.628930817610063No Hit
ATCTCTGGGGCTTCTTCCGCCCCCCCCGGCCTCCCCCCCC10.628930817610063No Hit
ATCTCTGGGTGCAACCCAGAGCAAAAAAACACACCACACC10.628930817610063No Hit
ATCTCTGGGAACAGTGGTATCAACCCAGAATTGACTTTTT10.628930817610063No Hit
ATCTCTGGGATGGTCTCACCGCAAAACAACACACACCAAC10.628930817610063No Hit
ATCTCTGGGAGCTCCATCCTTTTTGTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGATCACGAGCGCGTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGGCGTGCCGAGAGCCTTTGTCCGGTCCGCCGG10.628930817610063No Hit
ATCTCTGGGACAGTGGTATCAACACCAAAAACCACACCCA10.628930817610063No Hit
ATCTCTGGGATGGTCTACCCCAGAGTCCACTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGGTGCTATCTNTAAAAAAAAAAAAAAAANAAA10.628930817610063No Hit
ATCTCTGGGTTACGCCGAGTTGACTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGACAAAAAAACAAAAAAAAAAACACACAAAAA10.628930817610063No Hit
ATCTCTGGGGTGCACCGGCCGCCCCCCTCGGCCTGGTCGC10.628930817610063No Hit
ATCTCTTACCCAACACATCTAGCCAGTACAGAAAAGAATA10.628930817610063No Hit
ATCTCTGGGTTCAAGCGACTTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGCCCGTGGCCCCCAGAGCCGACTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGTTACGCCGACCACTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGGCTCTTTGGTTTCCGTTCCTGCCCCATGTGA10.628930817610063No Hit
ATCTCTGGGGTGACTTGTGTGTGGTAGAGGTTACTGTCAT10.628930817610063No Hit
ATCTCTGAGCGGGGAGAAATCAATCCCCAATCCTGCATAG10.628930817610063No Hit
ATCTCTGGGATATCAACGCAAAAAAAAAAAAAAAAAAAAA10.628930817610063No Hit
ATCTCTGGGCTACCCTTTTTAAAAAAAAAAAAAAAAAAAA10.628930817610063No Hit
ATCTCTTGTGGTTTTTTTGTTTGTTTTCTTTTGTTCTCAC10.628930817610063No Hit
ATCTCTGGGTCCACGCGGAGTCGACTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGGTCACCCCCCACCTTCCCCCCATGCCCGCCC10.628930817610063No Hit
ATCTCTGGGGCTATCCCTGGGAACTGTCCAAAAAAAAAAA10.628930817610063No Hit
ATCTCTGGGTGCGTGCCGTGCCATCTCTGGGCTGGTCACC10.628930817610063No Hit
ATCTCTGGGGTGCAGTGCTGAGATCGGGGACACGCCGGCC10.628930817610063No Hit
ATCTCTGGGACGCATGGCATCCCCCCATAGCCCCCTCTCC10.628930817610063No Hit
ATCTCTGGGGAGTGCAGTGTTATCTCTGGGCCATCTCATC10.628930817610063No Hit
ATCTCTGGGGCTTTCAAAAATACCCACCCAAAAAAAAACA10.628930817610063No Hit
ATCTCTGGGGTGCAGTGCTATCTCTGGGACATCTCAACGC10.628930817610063No Hit
ATCTCTGGGACATCAACCCCGAGTCCACTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGCAGCAGTGGTTCCCCCCGCGTCTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGAAGCATGGTATCAACGCCGCGCCGACTTTTT10.628930817610063No Hit
ATCTCTGGGAAGCAGTGGTTCACCCAGAGTCTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGAAGTTCCACCCGCGTCGACTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGTACCCCCAGTTCTGAGCCGGAGACCCGCCGG10.628930817610063No Hit
ATCTCTGGGCTGGTCCGAGCCCCCTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGGGGCTGATTTGGAAACAGGGATGGAACACCC10.628930817610063No Hit
ATCTCTGGGCGAGTGCAGTGCTATCCCTGGGCCGTGCTCT10.628930817610063No Hit
ATCTCTCCCCCCCCCCCCCCCCCCCTTTCGTGTTTGGCTT10.628930817610063No Hit
ATCTCTGGGAGCTCTTTCTCGATTCCGCGGGGGGTGGTGC10.628930817610063No Hit
ATCTCTGGGGAGTCGAATTTTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGCAGCAGTGGTATCAACCCCGTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGGCTTCTGGGCTCTAGTTTCATTTCTCCGGTT10.628930817610063No Hit
ATCTCTGGAGAAGCATTCCCACCCCCCCATTGCGTCCCTG10.628930817610063No Hit
ATCTCTGGGAAGCATGGTATCAACCCAAAAAAAATCGGAA10.628930817610063No Hit
ATCTCTGGGATATTCAACTGAGTAAAATCTTGTCATGTCA10.628930817610063No Hit
ATCTCTGGGGAAACCTCCCAAACAAACAAAACACACAAAA10.628930817610063No Hit
ATCTCTGGGAGTGCAGTGCTATCTCTGGGTGCGAGTCGCT10.628930817610063No Hit
ATCTCTGGGGATCAGTTGGTCACTGCACCCCTGACCCCCG10.628930817610063No Hit
ATCTCTGGGGGTGGTATCCACGCAAAAAAACAAACACAAA10.628930817610063No Hit
ATCTCTGGGGCCGAATCGCCTCCCCCCCCAACCGCCCGGA10.628930817610063No Hit
ATCTCTGGGGTGCTATCTCTGGGAAGCATGTAGATCGCCC10.628930817610063No Hit
ATCTCTGGGCTCTGCTGCTAGCCCCTTTCTCCGCTTCTGC10.628930817610063No Hit
ATCTCTGGCCACCAGCCTTACCCACACCCCCCTCCCTCCC10.628930817610063No Hit
ATCTCTGGGACTGCTTCCTTCCAAAAAAAAAAAAAAAAAA10.628930817610063No Hit
ATCTCTAGGCTTTCCCGCCGTCGACTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGAATCTCACCCAGCGCCCCCTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGTATCCGGGAGTCGCCTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGCTCAACAGCCCCATTTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGACCGACGGGGTTTTCGTTTTCTCCCTCTCTT10.628930817610063No Hit
ATCTCTGGAAGTGGATACCCGAGCCCCCCTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGAAGCATGGTACCAACCCAGAGTCGCCTTTTT10.628930817610063No Hit
ATCTCTGGGCCATCTCACCCCTCCCCCCCCCCCCCCCACA10.628930817610063No Hit
ATCTCTGGGGGCATTCGTGGAGCCAGACATGCCTCCCCCC10.628930817610063No Hit
ATCTCTGGCGCGTCCACTTTTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGAATGGTACCCCCCCCGAGCCGCCTTTTTTTT10.628930817610063No Hit
ATCTCTGGGAGCAGTTTTTTTCTTTTTTTTTTTTTTTCAC10.628930817610063No Hit
ATCTCTGGGTCAGAGCCATTTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGAGCATGAGGGCTGAATATTGCTCAGTATTAT10.628930817610063No Hit
ATCTCTGGTGCGAAACCAACAAAAAAAAAAAAAAAAAACA10.628930817610063No Hit
ATCTCTGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGATGGTCTCACCCCAACCAAAAACCAAACCCC10.628930817610063No Hit
ATCTCTGGGCAGCATGGTACGCCCCCGAGCCGCCTTTTTT10.628930817610063No Hit
ATCTCTGGGGTGCAGTGCTACCTCTGGGAGTGGTCCCGCA10.628930817610063No Hit
ATCTCTGGGTGGCCGAGTCGCTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGTCCAGCCTTCTTCCCCCCCAACACCAACACC10.628930817610063No Hit
ATCTCTGGGCTCGGAAGACCTCGTATGCCGTCTTCTGCTT10.628930817610063Illumina Single End Adapter 2 (96% over 30bp)
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGCAGACCC10.628930817610063No Hit
ATCTCTGGGACCAGTGCCAAACCACCACAACCACACCACC10.628930817610063No Hit
ATCTCTGGGACCAACTCGTTCCATAATAACACCTCGTCTG10.628930817610063No Hit
ATCTCTGGGGTGCAGTGCCATCCCTGGGAACCCGTGCTAT10.628930817610063No Hit
ATCTCTGGGAAGCAGTGGTACCCGCCGCGCCCACCCAAAC10.628930817610063No Hit
ATCTCTGGGGGTTTTTTCCGCCCCCCGGGTTCGTGTCTGC10.628930817610063No Hit
ATCTCTGGGTGGTTACCCCCACCGACTTTTCTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGAGGGACGGGGTTTTCGTTTTCTCTCTCTCTT10.628930817610063No Hit
ATCTCTGGGAAGCATGTACAACCCCCAGTCCCCTTTTTTT10.628930817610063No Hit
ATCTCTGGGTGCAGTGCTATCTCTGGGGGAAAGCCGGACT10.628930817610063No Hit
ATCTCTGGGTTATCAACCCCCCGTCCCCTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGAGCATGGTATCACCGCCGCGTCGACCTTTTT10.628930817610063No Hit
ATCTCTGGGGAGTGCCGTGCCCCTTCTGGGCTCGTCTTCT10.628930817610063No Hit
ATCTCTGGGTCGACTTTTTTTTTTTTTNTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTACACCC10.628930817610063No Hit
ATCTCTGGGCAGTCGCCGCGCCGACCTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGGTGCCCATTTGTGTTTTTCCCCCCCCGCCTC10.628930817610063No Hit
ATCTCTGGGCACATAACCCTTACTACTACTGTAACGCACT10.628930817610063No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGACCG10.628930817610063No Hit
ATCTCTGGGCGATGCGTCCGACCCTTGGGACCCCCCCCCT10.628930817610063No Hit
ATCTCTGGGACGCATGGTATCACCGAGCCGCCTCTTTTTT10.628930817610063No Hit
ATCTCTGGGCAGCAGTGGTCTCAACCCAGAGTCCCCTTCT10.628930817610063No Hit
ATCTCTGGGATAAACCCCCCGTCCCCTCTCTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGGGGACCCCTTTGTAGCCACAGAACCCACCAG10.628930817610063No Hit
ATCTCTGGGAGCTTGCGTTTGTGTGTGTGTGTGTGTGTGT10.628930817610063No Hit
ATCTCTGGGAACATCGCTTTTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGCTGGGAAGTGCAAAAAAAAAAAAACAAAAAA10.628930817610063No Hit
ATCTCTGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGAAGTAAAATGATTTTTTAAAACACCACGCCT10.628930817610063No Hit
ATCTCTGGGAGCAGTGGTCCACCCAGAGTCTTTTTTCTTT10.628930817610063No Hit
ATCTCTGGGTGCAGTGCTATCTCTGGGCTGACACCCCCCG10.628930817610063No Hit
ATCTCTGGGAGGGACGGGGTTTTCGTTTTCCCTCTCTCTT10.628930817610063No Hit
ATCTCTGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGGACATGGTCACCCCCCCCCCCCCCCCCCCCC10.628930817610063No Hit
ATCTCTGGGCCAGTACCAACCCCCCGTCGACTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGGGCAAAAAAAAAAAAAAAAAACACAAAAACA10.628930817610063No Hit
ATCTCTGGGAAGCATGGCCTCAACCCAGAGCCGCCTTTTT10.628930817610063No Hit
ATCTCTGGGTGGTATCAACGCAGGGATGAGATGGACTTCA10.628930817610063No Hit
ATCTCTGGGACGCAGTGGTTCAACACAGAAAAAACACAAC10.628930817610063No Hit
ATCTCTGGGGAAGATGGTATCCGCCGGCCACTTTTTTTTT10.628930817610063No Hit
ATCTCTGGGAGCAGTGGTACCCCCCGCGCCCTTTTTCTTT10.628930817610063No Hit
ATCTCTGGGCTCTCTCCTAACCCTGAATGTGAACACATGA10.628930817610063No Hit
ATCTCTGGGCCCGTGGTCACCGCCGCGCCGCCTTTTTTTT10.628930817610063No Hit
ATCTCTGGGGTGCTATCTCTGGGATGGCCACCCGAGTCGA10.628930817610063No Hit
ATCTCTGGGGCCTCTTTTTTTTTTTTTTTTTTTTTTTTTC10.628930817610063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGGG150.034.03
ATCTCTG150.034.01
TCTCTGG150.034.02
Produced by FastQC (version 0.11.5)