FastQCFastQC Report
Fri 17 Jun 2016
SRR1525871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT311.9935691318327973No Hit
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT231.4790996784565917No Hit
ATCTCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG161.0289389067524115No Hit
ATCTCTGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATT70.45016077170418006No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG70.45016077170418006No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC50.3215434083601286No Hit
ATCTCTGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA50.3215434083601286No Hit
ATCTCTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT40.2572347266881029No Hit
ATCTCTGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGA40.2572347266881029No Hit
ATCTCTGGGCAGTGCTATCTCTGGGTAACCAAGGCACACC40.2572347266881029No Hit
ATCTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.2572347266881029No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA40.2572347266881029No Hit
ATCTCTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT30.19292604501607716No Hit
ATCTCTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG30.19292604501607716No Hit
ATCTCTGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG30.19292604501607716No Hit
ATCTCTGGGAGCTGGGGTTGGTATGAGTCCAAGTGAGTCC30.19292604501607716No Hit
ATCTCTGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGA30.19292604501607716No Hit
ATCTCTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT30.19292604501607716No Hit
ATCTCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.19292604501607716No Hit
ATCTCTGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTT30.19292604501607716No Hit
ATCTCTGGGGGGGAGGTAGTGACGAAAAATAACAATACAG30.19292604501607716No Hit
ATCTCTGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT30.19292604501607716No Hit
ATCTCTGGGCATCAAGCTCCGCTCGGTGGCCAGCAGAGAG20.12861736334405144No Hit
ATCTCTGGGGACATCGTGCTTCACGATACATACTATGTAG20.12861736334405144No Hit
ATCTCTGGGATCAGAAACCGTTACAATTTCCTGTTGCTGT20.12861736334405144No Hit
ATCTCTGGGGGCAGTGGCTCCTGGAAGAGGAACAAGTGTG20.12861736334405144No Hit
ATCTCTGGGGATCCTCTTCTGAAGGAGATTGGGCGGATTG20.12861736334405144No Hit
ATCTCTGGGACTCACCAATATCCTCACAATATATCAATGA20.12861736334405144No Hit
ATCTCTGGGGGGCTCAGTGGAGTCATGTGGGAATCACTGA20.12861736334405144No Hit
ATCTCTGGGTATCAATGGGAGCAGTGTTTGCTATCATAGC20.12861736334405144No Hit
ATCTCTGGGGCCGCCTGGATACCGCAGCTAGGAATAATGG20.12861736334405144No Hit
ATCTCTGGGGAATGGGATGTGAACTCCACAGGAAGACCAA20.12861736334405144No Hit
ATCTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12861736334405144No Hit
ATCTCTGGGTTCGTAGGAGTAAACATAACATTCTTCCCTC20.12861736334405144No Hit
ATCTCTGGGATAGTTTCTCAGCTTCCCTTTCAGTTCTCCT20.12861736334405144No Hit
ATCTCTGGGTACCTGGTTGATACTGCCAGTAGCATATGCT20.12861736334405144No Hit
ATCTCTGGGTTCTTCTGAGATGAGTTTTTGTTCAATCCGC20.12861736334405144No Hit
ATCTCTGGGGCAGTGCTATCTCTGGGGTAATTATCCTAAG20.12861736334405144No Hit
ATCTCTGGGTAACAGATCCTCTTCTGAGATGAGTTTTTGT20.12861736334405144No Hit
ATCTCTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAA20.12861736334405144No Hit
ATCTCTGGGATTCACAGTAGGATTAGATGTAGACACACGA20.12861736334405144No Hit
ATCTCTGGGGGAGAGGGAGCCTGAGAAACGGCTAACACAT20.12861736334405144No Hit
ATCTCTGGGAGGCTCTATCCTTTTACTGACCAGTTAAATT20.12861736334405144No Hit
ATCTCTGGGCAGATTTCCTGGGTGAACAAAATTAGTAAAG20.12861736334405144No Hit
ATCTCTGGGACTAAAAGTATTGGAGAAAGAAATTCGTACA20.12861736334405144No Hit
ATCTCTGGAGTTCTCCATTTACCCAGCCCCCCAGGTTTCC20.12861736334405144No Hit
ATCTCTGGGCACAGTGATTCGACATTTTGTGTAACGAGAA20.12861736334405144No Hit
ATCTCTGGGGGGCTGTGCTTGTTTGTGGCTTAGATGGTAG20.12861736334405144No Hit
ATCTCTGGGGACTTGCTGGGTTTAATTCAAATTGTCTAAT20.12861736334405144No Hit
ATCTCTGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG20.12861736334405144No Hit
ATCTCTGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGC20.12861736334405144No Hit
ATCTCTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT20.12861736334405144No Hit
ATCTCTGGGGAGGCCCTGTAATTGGAATGAGTCCACTTTA20.12861736334405144No Hit
ATCTCTGGGGGGGAATCAGGGTTCGATTCCGGAGAGGGAG20.12861736334405144No Hit
ATCTCTGGGGTCTCAAAGATTAAGCCATGCATGTCTAAGT20.12861736334405144No Hit
ATCTCTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG20.12861736334405144No Hit
ATCTCTGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTT20.12861736334405144No Hit
ATCTCTGGGGAGCCACATTAACCGGTGGCCAGTTCTGGAA20.12861736334405144No Hit
ATCTCTGGGATATGTATGTTCATATGTGAGTGTGTGTATG20.12861736334405144No Hit
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC20.12861736334405144No Hit
ATCTCTGGGGGGGGCGAAGTTTGAAGAAGAAAACAAAGAA20.12861736334405144No Hit
ATCTCTGGGCTGGTTGATCCTGCCAGTAGCATATGCTTGT20.12861736334405144No Hit
ATCTCTGGGGGGCACCCGGAAAAGGAAATACACTGAGGTG20.12861736334405144No Hit
ATCTCTGGGATGCCATCTACCTTCTTCAACCTCACCATAG20.12861736334405144No Hit
ATCTCTGGGGGGAGGTAGTGACGAAAAATAACAATACAGG20.12861736334405144No Hit
ATCTCTGGGAAGCAAATCCTATAGTGCATGGACATTTGGA20.12861736334405144No Hit
ATCTCTGGGAGCATCTTCAGGTTTAACAGGGTCTGTACAC20.12861736334405144No Hit
ATCTCTGGGTTGACATCGTGCTTCACGATACATACTATGT20.12861736334405144No Hit
ATCTCTGGGCACTTGTCTGCCCCAGCAAAACATACGACAA20.12861736334405144No Hit
ATCTCTGGGCCAAGATCTTCTGTTTGGGGTTACATAATCA20.12861736334405144No Hit
ATCTCTGGGATTGAAATAATTACCGATAGGCAGTCTGGAA20.12861736334405144No Hit
ATCTCTGGGCTCTGGGTCAGGTCTAAGTGAAGCTTCCTAG20.12861736334405144No Hit
ATCTCTGGGCAGGTGACGAATACAGTGAGGAGGAAGAGTC20.12861736334405144No Hit
ATCTCTGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGT20.12861736334405144No Hit
ATCTCTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12861736334405144No Hit
ATCTCTGGGGAAATGGCTCAGTGGTTAAGAGCACTGTCTG20.12861736334405144No Hit
ATCTCTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAAC20.12861736334405144No Hit
ATCTCTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGA20.12861736334405144No Hit
ATCTCTGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12861736334405144No Hit
ATCTCTGGGGTGCTATCTCTGGGATGCCATCTACCTTCTT20.12861736334405144No Hit
ATCTCTGGGGTGCTATCTCTGGGAGAGAAACCCTGTCTCA20.12861736334405144No Hit
ATCTCTGGGTGCGGGTTGGGTTCCAGATTAGACATTAGAA20.12861736334405144No Hit
ATCTCTGGGGTGTTCTTTATTAATATCCTAACACTCCTCG20.12861736334405144No Hit
ATCTCTGGGTGGAATATCTTGTTTTCTTCATCTATGGTGA20.12861736334405144No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGGG200.002018422434.05
CTGGGGA251.2417891E-434.05
TCTGGGT200.002018422434.04
TCTGGGC200.002018422434.04
TCTGGGA307.4042455E-634.04
CTCTGGG1500.032.8666653
ATCTCTG1650.031.9393921
TCTCTGG1650.031.9393922
TCTGGGG800.031.8754
CTGGGGT250.00603428527.1999995
Produced by FastQC (version 0.11.5)