FastQCFastQC Report
Fri 17 Jun 2016
SRR1525870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT1123.679369250985545No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC1103.613666228646518No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC280.9198423127463863No Hit
ATGGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA250.821287779237845No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.6241787122207622No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATATCGTATGCCGT180.5913272010512484No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT160.5256241787122208Illumina Single End Adapter 2 (95% over 24bp)
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT130.42706964520367935No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA120.39421813403416556No Hit
ATGGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGCTGTAGCGCCGGAAGA120.39421813403416556No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCTAATCCGCCCAATCT120.39421813403416556No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC120.39421813403416556No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA110.36136662286465177No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGAGCTCGTATGCCGT110.36136662286465177Illumina Single End Adapter 1 (95% over 23bp)
ATGGCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG90.2956636005256242No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGATATCGTATGCCGT90.2956636005256242No Hit
ATGGCGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC80.2628120893561104No Hit
ATGGCGGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC70.22996057818659657No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTTAATCCGCCCAATCT70.22996057818659657No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC70.22996057818659657No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATTCGCCCAATCT70.22996057818659657No Hit
ATGGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCCTTGTAGCGCCGGAAGA60.19710906701708278No Hit
ATGGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAAA60.19710906701708278No Hit
ATGGCGGGGTCACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCGGAAG50.164257555847569Illumina Single End Adapter 2 (100% over 34bp)
ATGGCGGGGTCACAGATCCTCTCCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT50.164257555847569No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGATCTCGTATGCCGT50.164257555847569No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATCTCGTATGCCGT50.164257555847569Illumina Single End Adapter 1 (95% over 23bp)
ATGGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT40.1314060446780552No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGTACAAAAACTC40.1314060446780552No Hit
ATGGCGGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC40.1314060446780552No Hit
ATGGCGAGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT40.1314060446780552No Hit
ATGGCGGGGTCCCCCCTCCTCTTCTGAGATGAGTTTTTGTTCCCTCCGCCCAATCT40.1314060446780552No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCTAATCCGCCCAAAAA40.1314060446780552No Hit
ATGGCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA40.1314060446780552No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCCATTGAACAAAAACTC40.1314060446780552No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA40.1314060446780552No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA40.1314060446780552No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGCCGAGCTCGTATGCCGT40.1314060446780552No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTT40.1314060446780552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT1350.050.0000044
GCGGGGC203.5472712E-450.04
GCGGGGA550.050.04
CCAATCT251.6275591E-550.050
CGGGGTC1300.050.05
CGGGGGG401.4606485E-950.05
AGGATCG203.5472712E-450.032
CGGGGAG203.5472712E-450.05
GAAGCAG203.5472712E-450.09
GAGCTGT203.5472712E-450.09
GCGGATT203.5472712E-450.038
GATGAGT501.8189894E-1250.028
GATGAGG251.6275591E-550.028
GGGAGCT203.5472712E-450.07
ACCGACC203.5472712E-450.018
GGAAGCA203.5472712E-450.08
ATTGAAC251.6275591E-550.042
TGAGGAT203.5472712E-450.030
GGGGTCC203.5472712E-450.06
GGGAAGC203.5472712E-450.07
Produced by FastQC (version 0.11.5)