##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525870_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3044
Sequences flagged as poor quality	0
Sequence length	56
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.58508541392904	38.0	36.0	39.0	33.0	39.0
2	35.78022339027595	38.0	36.0	39.0	31.0	39.0
3	35.422470433639944	38.0	35.0	39.0	30.0	39.0
4	35.542049934296976	38.0	35.0	39.0	30.0	39.0
5	35.584756898817346	38.0	35.0	39.0	30.0	39.0
6	35.539093298291725	38.0	35.0	39.0	30.0	39.0
7	35.851839684625496	38.0	36.0	39.0	33.0	39.0
8	34.434954007884365	35.0	35.0	39.0	30.0	39.0
9	34.23554533508541	35.0	35.0	39.0	29.0	39.0
10	32.90144546649146	35.0	32.0	38.0	24.0	39.0
11	32.41951379763469	35.0	31.0	38.0	22.0	39.0
12	32.133377135348226	35.0	31.0	38.0	20.0	39.0
13	30.10052562417871	33.0	27.0	37.0	14.0	38.0
14	32.380749014454665	35.0	31.0	38.0	21.0	39.0
15	28.29927726675427	31.0	25.0	35.0	10.0	38.0
16	29.691524310118265	33.0	27.0	37.0	13.0	38.0
17	27.17148488830486	31.0	21.0	35.0	10.0	38.0
18	28.107752956636006	32.0	23.0	36.0	11.0	38.0
19	29.71222076215506	33.0	26.0	38.0	13.0	38.0
20	29.577529566360052	33.0	26.0	36.0	15.0	38.0
21	31.246714848883048	35.0	29.0	38.0	15.0	39.0
22	30.469119579500656	35.0	29.0	38.0	13.0	38.0
23	31.6080814717477	36.0	30.0	38.0	15.0	39.0
24	29.873193166885677	34.0	27.0	37.0	10.0	38.0
25	30.988830486202364	35.0	29.0	38.0	10.0	38.0
26	28.85282522996058	33.0	26.0	36.0	7.0	38.0
27	29.042706964520367	33.0	26.0	36.0	4.0	38.0
28	30.229960578186596	35.0	29.0	38.0	4.0	38.0
29	28.996386333771355	33.0	27.0	36.0	4.0	38.0
30	29.102496714848883	33.0	27.0	37.0	4.0	38.0
31	28.019382391590014	33.0	24.0	36.0	4.0	38.0
32	28.68626806833114	33.0	26.0	36.0	4.0	38.0
33	27.92706964520368	33.0	24.0	36.0	4.0	38.0
34	26.811103810775297	31.0	22.0	35.0	4.0	38.0
35	28.333442838370566	33.0	26.0	36.0	4.0	38.0
36	26.714520367936924	32.0	21.0	36.0	4.0	38.0
37	26.64323258869908	33.0	19.0	36.0	4.0	38.0
38	26.38994743758213	33.0	16.0	36.0	4.0	38.0
39	24.066360052562416	30.0	4.0	35.0	4.0	38.0
40	22.52365308804205	29.0	4.0	35.0	4.0	38.0
41	20.303547963206306	25.0	4.0	33.0	4.0	36.0
42	19.11629434954008	21.0	4.0	32.0	4.0	36.0
43	20.68626806833114	25.0	4.0	33.0	4.0	37.0
44	20.80814717477004	25.0	4.0	34.0	4.0	38.0
45	20.442181340341655	25.0	4.0	33.0	4.0	36.0
46	18.77168199737188	19.0	4.0	32.0	4.0	36.0
47	18.936925098554532	20.0	4.0	33.0	4.0	36.0
48	18.740801576872535	19.0	4.0	32.0	4.0	36.0
49	18.42772667542707	18.0	4.0	32.0	4.0	36.0
50	19.403745072273324	23.0	4.0	33.0	4.0	37.0
51	19.724047306176082	23.0	4.0	35.0	4.0	38.0
52	19.31438896189225	23.0	4.0	34.0	4.0	37.0
53	19.369250985545335	23.0	4.0	34.0	4.0	37.0
54	18.867279894875164	20.0	4.0	34.0	4.0	37.0
55	19.29599211563732	22.0	4.0	35.0	4.0	38.0
56	17.810446780551906	4.0	4.0	33.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.992477876106193
1	2	4.217109144542768
1	3	4.072271386430678
1	4	4.27389380530974
1	5	3.4205014749262475
1	6	3.9560471976401104
1	7	3.7926253687315494
1	8	4.356489675516222
1	9	4.115486725663715
1	10	4.612684365781707
1	11	-1.5144542772861342
1	12	-9.732005899705015
1	13	-19.655899705014754
1	14	-3.9289085545722813
1	15	3.264749262536874
1	16	1.018289085545721
1	17	-8.829646017699122
1	18	-1.6802359882005966
1	19	1.0808259587020679
1	20	1.8280235988200602
1	21	4.651327433628321
1	22	3.5820058997050097
1	23	5.352949852507379
1	24	4.463126843657815
1	25	3.7592920353982286
1	26	3.6306784660766986
1	27	-0.4342182890855497
1	28	4.145427728613566
1	29	0.6997050147492665
1	30	-5.316814159292033
1	31	-6.193510324483771
1	32	-4.581415929203548
1	33	-0.928318584070805
1	34	-1.5619469026548636
1	35	-0.889675516224198
1	36	2.3855457227138643
1	37	2.1999999999999957
1	38	3.187463126843653
1	39	-9.855014749262534
1	40	-8.742477876106202
1	41	-10.761209439528022
1	42	-9.73864306784661
1	43	-12.33480825958702
1	44	-14.111799410029496
1	45	-11.86946902654867
1	46	-9.362094395280234
1	47	-11.161209439528022
1	48	-8.347787610619463
1	49	-11.348525073746309
1	50	-10.60486725663717
1	51	-6.769911504424778
1	52	-7.114306784660764
1	53	-6.526401179940999
1	54	-5.624336283185842
1	55	-6.4731563421828895
1	56	-5.459292035398226
2	1	3.6591445427728573
2	2	5.083775811209435
2	3	5.738938053097343
2	4	4.973893805309743
2	5	3.7871681415929146
2	6	5.222713864306776
2	7	4.659292035398217
2	8	5.423156342182885
2	9	5.515486725663713
2	10	5.212684365781708
2	11	1.9522123893805308
2	12	-0.36533923303834825
2	13	-3.089233038348084
2	14	3.704424778761055
2	15	5.298082595870206
2	16	-1.7817109144542798
2	17	4.137020648967546
2	18	-3.446902654867266
2	19	2.0141592920354014
2	20	0.7613569321533937
2	21	-0.11533923303834825
2	22	1.6153392330383411
2	23	-0.9803834808259531
2	24	0.1297935103244825
2	25	0.9259587020648965
2	26	3.697345132743365
2	27	2.7657817109144496
2	28	2.445427728613563
2	29	2.666371681415935
2	30	-1.4168141592920342
2	31	-1.993510324483772
2	32	-2.91474926253688
2	33	-1.1949852507374743
2	34	-1.5286135693215321
2	35	-2.4563421828908645
2	36	1.4188790560471958
2	37	2.6666666666666643
2	38	2.854129793510321
2	39	4.1449852507374665
2	40	-1.5091445427728694
2	41	-0.0278761061946895
2	42	-0.7386430678466098
2	43	1.4318584070796483
2	44	-3.878466076696162
2	45	1.863864306784663
2	46	0.7379056047197654
2	47	-1.6278761061946891
2	48	1.7522123893805368
2	49	-6.081858407079643
2	50	0.06179941002949718
2	51	1.4634218289085545
2	52	-0.21430678466076358
2	53	1.8735988200590015
2	54	1.2089970501474898
2	55	2.0268436578171105
2	56	4.407374631268441
3	1	2.492477876106193
3	2	0.9171091445427706
3	3	2.905604719764014
3	4	1.9738938053097428
3	5	1.7871681415929146
3	6	3.222713864306776
3	7	1.1592920353982166
3	8	2.5898230088495566
3	9	0.01548672566371323
3	10	1.0460176991150405
3	11	0.7855457227138665
3	12	-0.032005899705016105
3	13	4.744100294985248
3	14	3.3710914454277194
3	15	-5.868584070796459
3	16	5.3849557522123845
3	17	5.637020648967546
3	18	6.886430678466066
3	19	6.514159292035405
3	20	4.594690265486726
3	21	6.217994100294984
3	22	5.782005899705009
3	23	3.352949852507379
3	24	4.79646017699115
3	25	5.425958702064893
3	26	4.697345132743365
3	27	4.76578171091445
3	28	3.778761061946895
3	29	2.166371681415935
3	30	7.4165191740413015
3	31	3.33982300884956
3	32	6.585250737463117
3	33	5.638348082595858
3	34	3.8047197640118036
3	35	5.043657817109139
3	36	5.2522123893805315
3	37	3.6666666666666643
3	38	9.187463126843653
3	39	6.811651917404134
3	40	8.49085545722713
3	41	14.805457227138643
3	42	15.76135693215339
3	43	11.76519174041298
3	44	8.288200589970504
3	45	14.863864306784667
3	46	17.237905604719764
3	47	15.038790560471977
3	48	18.085545722713874
3	49	15.084808259587023
3	50	11.061799410029497
3	51	9.79675516224189
3	52	9.452359882005904
3	53	13.206932153392334
3	54	9.875663716814158
3	55	7.0268436578171105
3	56	7.407374631268441
4	1	3.492477876106193
4	2	4.917109144542771
4	3	5.072271386430678
4	4	4.8072271386430785
4	5	4.62050147492625
4	6	4.222713864306776
4	7	4.325958702064888
4	8	4.589823008849557
4	9	4.1821533923303775
4	10	4.546017699115044
4	11	4.118879056047202
4	12	0.801327433628316
4	13	5.077433628318584
4	14	4.204424778761055
4	15	3.4647492625368734
4	16	4.551622418879056
4	17	7.470353982300878
4	18	7.553097345132738
4	19	6.847492625368734
4	20	5.261356932153397
4	21	6.217994100294984
4	22	7.282005899705009
4	23	5.186283185840715
4	24	6.629793510324479
4	25	7.592625368731564
4	26	5.864011799410029
4	27	4.932448377581114
4	28	2.1120943952802307
4	29	6.1663716814159315
4	30	4.416519174041298
4	31	2.173156342182896
4	32	2.08525073746312
4	33	2.6383480825958614
4	34	5.304719764011804
4	35	2.7103244837758034
4	36	5.418879056047196
4	37	6.166666666666664
4	38	6.354129793510321
4	39	6.1449852507374665
4	40	4.324188790560466
4	41	12.97212389380531
4	42	13.594690265486722
4	43	11.598525073746313
4	44	12.121533923303836
4	45	13.03053097345133
4	46	14.571238938053098
4	47	13.705457227138645
4	48	12.418879056047205
4	49	14.584808259587023
4	50	14.39513274336283
4	51	13.630088495575222
4	52	12.952359882005904
4	53	12.706932153392334
4	54	12.875663716814158
4	55	13.693510324483778
4	56	14.907374631268441
5	1	3.3924778761061916
5	2	4.417109144542771
5	3	4.8722713864306755
5	4	3.8738938053097414
5	5	1.8205014749262531
5	6	3.9560471976401104
5	7	3.7926253687315494
5	8	3.9564896755162238
5	9	3.915486725663712
5	10	1.012684365781709
5	11	2.2855457227138665
5	12	1.2679941002949846
5	13	4.944100294985251
5	14	4.671091445427717
5	15	5.864749262536872
5	16	6.618289085545719
5	17	8.770353982300875
5	18	10.5197640117994
5	19	4.680825958702069
5	20	6.428023598820062
5	21	6.05132743362832
5	22	5.782005899705009
5	23	5.9529498525073805
5	24	5.063126843657816
5	25	7.35929203539823
5	26	7.2306784660767
5	27	1.9657817109144489
5	28	1.9454277286135628
5	29	6.899705014749266
5	30	4.8831858407079665
5	31	5.6064896755162295
5	32	6.418584070796452
5	33	6.271681415929191
5	34	-0.3619469026548643
5	35	4.510324483775804
5	36	6.985545722713862
5	37	9.399999999999995
5	38	9.387463126843656
5	39	9.544985250737465
5	40	11.2575221238938
5	41	12.438790560471979
5	42	8.861356932153392
5	43	12.065191740412981
5	44	15.288200589970504
5	45	16.53053097345133
5	46	6.837905604719767
5	47	11.838790560471978
5	48	11.652212389380535
5	49	10.851474926253692
5	50	12.19513274336283
5	51	5.830088495575222
5	52	2.4856932153392357
5	53	2.0735988200590008
5	54	3.975663716814159
5	55	6.72684365781711
5	56	7.740707964601773
6	1	3.79247787610619
6	2	5.217109144542768
6	3	5.8722713864306755
6	4	5.27389380530974
6	5	5.220501474926252
6	6	5.356047197640109
6	7	4.792625368731549
6	8	4.356489675516222
6	9	5.515486725663713
6	10	6.212684365781708
6	11	5.2855457227138665
6	12	6.467994100294984
6	13	5.744100294985248
6	14	3.0710914454277223
6	15	9.664749262536876
6	16	8.21828908554572
6	17	9.970353982300878
6	18	7.519764011799399
6	19	8.480825958702066
6	20	6.82802359882006
6	21	6.451327433628318
6	22	6.38200589970501
6	23	1.5529498525073784
6	24	0.26312684365781536
6	25	2.1592920353982272
6	26	-0.9693215339233028
6	27	-2.634218289085549
6	28	0.5454277286135643
6	29	-1.1002949852507342
6	30	1.6831858407079672
6	31	-2.993510324483772
6	32	-2.3814159292035484
6	33	-3.928318584070805
6	34	-8.161946902654865
6	35	-4.289675516224197
6	36	2.985545722713862
6	37	2.1999999999999957
6	38	2.187463126843653
6	39	4.744985250737468
6	40	-3.7424778761062
6	41	7.638790560471978
6	42	6.061356932153391
6	43	5.065191740412981
6	44	9.088200589970505
6	45	5.930530973451329
6	46	9.637905604719764
6	47	12.238790560471976
6	48	6.252212389380537
6	49	10.25147492625369
6	50	10.19513274336283
6	51	2.8300884955752217
6	52	4.285693215339236
6	53	6.273598820059
6	54	6.775663716814156
6	55	0.9268436578171091
6	56	7.540707964601774
7	1	3.5924778761061944
7	2	4.817109144542769
7	3	4.8722713864306755
7	4	4.873893805309741
7	5	3.6205014749262503
7	6	5.156047197640113
7	7	4.592625368731554
7	8	3.556489675516218
7	9	4.915486725663712
7	10	4.412684365781711
7	11	6.685545722713865
7	12	6.467994100294984
7	13	8.544100294985245
7	14	7.671091445427717
7	15	9.664749262536876
7	16	10.018289085545717
7	17	10.370353982300877
7	18	11.5197640117994
7	19	9.080825958702068
7	20	9.628023598820064
7	21	8.851327433628317
7	22	5.182005899705008
7	23	8.152949852507376
7	24	5.063126843657816
7	25	9.559292035398226
7	26	9.430678466076696
7	27	8.165781710914448
7	28	5.345427728613561
7	29	3.0997050147492686
7	30	10.083185840707966
7	31	6.406489675516227
7	32	7.818584070796451
7	33	7.671681415929196
7	34	5.038053097345134
7	35	5.710324483775803
7	36	8.585545722713864
7	37	10.599999999999998
7	38	12.587463126843652
7	39	11.744985250737468
7	40	9.057522123893797
7	41	15.038790560471977
7	42	16.061356932153387
7	43	15.865191740412982
7	44	16.888200589970506
7	45	15.93053097345133
7	46	17.637905604719762
7	47	20.438790560471976
7	48	17.052212389380536
7	49	15.45147492625369
7	50	15.795132743362828
7	51	19.630088495575222
7	52	16.685693215339235
7	53	14.273598820059
7	54	15.775663716814156
7	55	14.126843657817112
7	56	5.3407079646017745
8	1	2.242477876106193
8	2	3.6671091445427706
8	3	4.322271386430678
8	4	3.723893805309743
8	5	3.8705014749262503
8	6	3.806047197640112
8	7	3.2426253687315523
8	8	3.006489675516221
8	9	2.7654867256637132
8	10	1.9626843657817084
8	11	5.2855457227138665
8	12	4.717994100294984
8	13	7.494100294985248
8	14	2.8710914454277194
8	15	6.714749262536873
8	16	2.4682890855457202
8	17	7.220353982300878
8	18	6.719764011799402
8	19	4.680825958702069
8	20	6.678023598820062
8	21	4.80132743362832
8	22	6.282005899705009
8	23	-0.6470501474926209
8	24	-1.5368731563421854
8	25	3.7592920353982286
8	26	7.530678466076697
8	27	3.7657817109144496
8	28	0.9454277286135628
8	29	6.749705014749267
8	30	6.583185840707966
8	31	2.256489675516228
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114	1	3.3258112094395287
114	2	4.750442477876106
114	3	4.072271386430678
114	4	4.8072271386430785
114	5	4.62050147492625
114	6	4.8893805309734475
114	7	4.325958702064888
114	8	4.089823008849557
114	9	4.515486725663713
114	10	5.546017699115044
114	11	2.2855457227138665
114	12	1.8013274336283196
114	13	-0.2558997050147518
114	14	-1.4622418879056127
114	15	6.798082595870209
114	16	-2.7817109144542798
114	17	7.30368731563421
114	18	2.053097345132734
114	19	-0.3191740412979307
114	20	-0.9053097345132706
114	21	2.3846607669616517
114	22	6.115339233038345
114	23	5.352949852507379
114	24	2.4631268436578146
114	25	-0.9073746312684392
114	26	4.697345132743365
114	27	1.7657817109144496
114	28	2.945427728613563
114	29	2.499705014749267
114	30	3.416519174041298
114	31	3.006489675516228
114	32	5.085250737463117
114	33	5.805014749262529
114	34	6.304719764011804
114	35	0.7103244837758034
114	36	12.585545722713864
114	37	10.999999999999996
114	38	10.854129793510317
114	39	13.478318584070802
114	40	2.9908554572271306
114	41	4.9721238938053105
114	42	7.26135693215339
114	43	-3.4014749262536856
114	44	-4.711799410029496
114	45	-3.1361356932153353
114	46	-6.762094395280235
114	47	-9.961209439528023
114	48	-6.747787610619463
114	49	-9.415191740412975
114	50	-5.604867256637171
114	51	-4.036578171091444
114	52	-4.38097345132743
114	53	-3.7930678466076646
114	54	-3.6243362831858423
114	55	-4.139823008849556
114	56	-0.5926253687315608
115	1	3.3258112094395287
115	2	4.750442477876106
115	3	5.405604719764014
115	4	4.8072271386430785
115	5	4.953834808259586
115	6	4.556047197640112
115	7	4.325958702064888
115	8	4.089823008849557
115	9	3.848820058997049
115	10	5.546017699115044
115	11	-3.3811209439528014
115	12	-3.8653392330383483
115	13	-11.589233038348084
115	14	-3.7955752212389484
115	15	-5.868584070796459
115	16	3.5516224188790524
115	17	4.30368731563421
115	18	1.3864306784660698
115	19	1.6808259587020693
115	20	-2.9053097345132706
115	21	6.717994100294984
115	22	8.115339233038345
115	23	6.686283185840715
115	24	8.463126843657815
115	25	7.759292035398229
115	26	11.030678466076697
115	27	8.76578171091445
115	28	6.2787610619468985
115	29	8.166371681415932
115	30	7.74985250737463
115	31	8.006489675516228
115	32	6.085250737463117
115	33	10.138348082595858
115	34	8.638053097345136
115	35	8.376991150442468
115	36	11.9188790560472
115	37	0.9999999999999964
115	38	1.854129793510321
115	39	4.811651917404134
115	40	3.6575221238937985
115	41	5.638790560471978
115	42	5.26135693215339
115	43	2.2651917404129804
115	44	-3.7117994100294958
115	45	4.530530973451331
115	46	0.9045722713864333
115	47	-5.961209439528023
115	48	2.252212389380537
115	49	-2.7485250737463094
115	50	0.39513274336282933
115	51	4.630088495575222
115	52	1.6190265486725686
115	53	5.540265486725669
115	54	8.042330383480826
115	55	2.8601769911504427
115	56	9.740707964601773
116	1	2.6591445427728573
116	2	4.417109144542771
116	3	4.072271386430678
116	4	1.140560471976407
116	5	3.953834808259586
116	6	4.556047197640112
116	7	1.9926253687315523
116	8	5.089823008849557
116	9	0.8488200589970489
116	10	-1.1206489675516238
116	11	5.952212389380531
116	12	5.467994100294984
116	13	8.410766961651913
116	14	7.537758112094384
116	15	4.798082595870206
116	16	7.551622418879056
116	17	0.30368731563421036
116	18	0.3864306784660698
116	19	-0.9858407079645986
116	20	-1.2386430678466063
116	21	-1.2820058997050126
116	22	-0.8846607669616589
116	23	-1.980383480825953
116	24	-0.8702064896755175
116	25	-0.5740412979351035
116	26	0.36401179941002937
116	27	-1.5675516224188826
116	28	-0.721238938053105
116	29	-0.16696165191740064
116	30	-1.2501474926253664
116	31	0.3398230088495602
116	32	-1.2480825958702155
116	33	0.8050147492625257
116	34	-1.0286135693215321
116	35	-0.9563421828908645
116	36	2.5855457227138636
116	37	2.6666666666666643
116	38	3.520796460176985
116	39	4.811651917404134
116	40	5.6575221238937985
116	41	4.638790560471978
116	42	-1.7386430678466098
116	43	-0.7348082595870196
116	44	5.621533923303836
116	45	0.863864306784663
116	46	1.5712389380530976
116	47	0.03879056047197693
116	48	2.585545722713869
116	49	3.9181415929203585
116	50	1.3951327433628293
116	51	9.29675516224189
116	52	6.619026548672569
116	53	8.54026548672567
116	54	9.375663716814158
116	55	7.860176991150443
116	56	10.074041297935105
117	1	2.6591445427728573
117	2	4.083775811209435
117	3	4.405604719764014
117	4	4.140560471976407
117	5	4.287168141592915
117	6	3.8893805309734475
117	7	3.325958702064888
117	8	2.756489675516221
117	9	4.848820058997049
117	10	2.2126843657817084
117	11	1.9522123893805308
117	12	-3.532005899705016
117	13	3.410766961651916
117	14	1.2044247787610516
117	15	0.13141592920354128
117	16	-6.78171091445428
117	17	4.30368731563421
117	18	5.719764011799402
117	19	4.680825958702069
117	20	-0.5719764011799384
117	21	0.38466076696165175
117	22	7.115339233038345
117	23	6.019616519174043
117	24	8.463126843657815
117	25	-0.24070796460177135
117	26	10.030678466076697
117	27	2.7657817109144496
117	28	5.2787610619468985
117	29	5.499705014749267
117	30	3.416519174041298
117	31	6.673156342182892
117	32	3.4185840707964523
117	33	2.8050147492625257
117	34	3.971386430678468
117	35	4.710324483775803
117	36	6.2522123893805315
117	37	11.333333333333332
117	38	11.520796460176989
117	39	15.144985250737466
117	40	11.990855457227134
117	41	12.305457227138646
117	42	11.594690265486722
117	43	6.931858407079648
117	44	0.6215339233038364
117	45	9.863864306784663
117	46	8.237905604719765
117	47	12.372123893805309
117	48	-0.41445427728612927
117	49	3.2514749262536906
117	50	6.395132743362829
117	51	17.296755162241887
117	52	13.285693215339236
117	53	7.8735988200590015
117	54	16.042330383480824
117	55	11.193510324483778
117	56	13.074041297935105
118	1	3.6591445427728573
118	2	5.083775811209435
118	3	5.072271386430678
118	4	5.140560471976407
118	5	5.287168141592915
118	6	5.222713864306776
118	7	4.659292035398217
118	8	5.423156342182885
118	9	6.1821533923303775
118	10	5.879351032448373
118	11	2.9522123893805308
118	12	7.134660766961648
118	13	8.410766961651913
118	14	5.537758112094384
118	15	-5.868584070796459
118	16	0.21828908554572024
118	17	0.9703539823008782
118	18	-1.6135693215339302
118	19	1.3474926253687372
118	20	-0.23864306784660627
118	21	6.05132743362832
118	22	6.448672566371673
118	23	7.352949852507379
118	24	6.79646017699115
118	25	8.092625368731564
118	26	7.6973451327433615
118	27	7.76578171091445
118	28	9.945427728613563
118	29	9.166371681415932
118	30	10.083185840707966
118	31	9.339823008849564
118	32	8.418584070796452
118	33	9.80501474926253
118	34	10.638053097345136
118	35	8.710324483775803
118	36	12.585545722713864
118	37	12.333333333333332
118	38	12.520796460176989
118	39	15.144985250737466
118	40	15.657522123893798
118	41	0.9721238938053105
118	42	0.2613569321533902
118	43	-1.4014749262536874
118	44	3.6215339233038364
118	45	-7.136135693215335
118	46	-11.095427728613569
118	47	-6.294542772861357
118	48	-9.081120943952797
118	49	-5.748525073746309
118	50	-7.938200589970505
118	51	-3.7032448377581115
118	52	-4.714306784660764
118	53	-2.7930678466076646
118	54	-2.9576696165191763
118	55	-3.1398230088495556
118	56	0.4073746312684392
119	1	3.3258112094395287
119	2	5.083775811209435
119	3	5.738938053097343
119	4	5.140560471976407
119	5	5.287168141592915
119	6	4.8893805309734475
119	7	4.325958702064888
119	8	4.756489675516221
119	9	4.515486725663713
119	10	4.546017699115044
119	11	0.28554572271386647
119	12	-1.8653392330383483
119	13	0.07743362831858036
119	14	-6.795575221238948
119	15	2.1314159292035413
119	16	2.5516224188790524
119	17	5.637020648967546
119	18	0.719764011799402
119	19	4.0141592920354014
119	20	3.4280235988200616
119	21	7.384660766961655
119	22	8.782005899705009
119	23	8.019616519174043
119	24	3.796460176991147
119	25	7.759292035398229
119	26	6.364011799410033
119	27	8.099115044247785
119	28	8.278761061946899
119	29	10.833038348082603
119	30	7.74985250737463
119	31	8.673156342182892
119	32	6.751917404129788
119	33	9.80501474926253
119	34	8.3047197640118
119	35	6.043657817109139
119	36	13.252212389380528
119	37	13.66666666666666
119	38	13.854129793510317
119	39	11.81165191740413
119	40	15.324188790560463
119	41	13.638790560471978
119	42	-0.07197640117994197
119	43	15.598525073746316
119	44	14.954867256637169
119	45	16.863864306784667
119	46	7.904572271386433
119	47	12.038790560471977
119	48	8.585545722713869
119	49	7.584808259587023
119	50	7.7284660766961615
119	51	13.630088495575222
119	52	14.285693215339236
119	53	16.540265486725666
119	54	16.042330383480824
119	55	14.52684365781711
119	56	19.740707964601775
120	1	0.7924778761061901
120	2	2.017109144542772
120	3	0.07227138643067832
120	4	0.4738938053097428
120	5	0.020501474926248875
120	6	-0.04395280235988963
120	7	-1.0073746312684477
120	8	1.1564896755162195
120	9	0.3154867256637104
120	10	2.0126843657817055
120	11	-6.7144542772861335
120	12	-15.732005899705015
120	13	-4.855899705014753
120	14	-4.32890855457228
120	15	-7.335250737463127
120	16	-10.78171091445428
120	17	-1.6296460176991232
120	18	-2.6802359882005966
120	19	-6.319174041297931
120	20	-5.971976401179937
120	21	-3.748672566371681
120	22	-2.217994100294991
120	23	-2.4470501474926216
120	24	-0.33687315634218606
120	25	-3.040707964601772
120	26	-2.169321533923302
120	27	-3.4342182890855497
120	28	-4.054572271386437
120	29	-1.5002949852507328
120	30	-1.9168141592920342
120	31	0.20648967551622732
120	32	-1.781415929203547
120	33	-1.3283185840708072
120	34	-4.361946902654864
120	35	-3.0896755162241973
120	36	2.5855457227138636
120	37	-2.8000000000000043
120	38	-2.4125368731563483
120	39	-1.255014749262532
120	40	-5.542477876106201
120	41	-13.361209439528022
120	42	-12.73864306784661
120	43	-13.73480825958702
120	44	-14.711799410029496
120	45	-13.46946902654867
120	46	-11.762094395280235
120	47	-11.961209439528023
120	48	-11.747787610619463
120	49	-11.74852507374631
120	50	-12.60486725663717
120	51	-12.369911504424778
120	52	-12.714306784660764
120	53	-12.126401179940999
120	54	-11.624336283185842
120	55	-12.47315634218289
120	56	-11.259292035398227
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	113.0
5	1.0
6	7.0
7	8.0
8	32.0
9	28.0
10	20.0
11	21.0
12	13.0
13	17.0
14	22.0
15	24.0
16	31.0
17	40.0
18	49.0
19	63.0
20	72.0
21	99.0
22	122.0
23	118.0
24	120.0
25	133.0
26	112.0
27	86.0
28	116.0
29	144.0
30	158.0
31	167.0
32	203.0
33	203.0
34	206.0
35	177.0
36	153.0
37	115.0
38	39.0
39	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	98.29172141918528	1.5111695137976346	0.164257555847569	0.0328515111695138
8	98.25886990801577	1.1169513797634691	0.45992115637319314	0.164257555847569
9	94.67805519053877	1.6754270696452036	2.463863337713535	1.1826544021024967
10	21.583442838370566	16.557161629434955	54.82917214191853	7.030223390275952
11	18.462549277266753	19.842312746386334	5.519053876478318	56.176084099868596
12	21.81340341655716	54.89487516425755	5.847568988173456	17.44415243101183
13	9.034165571616294	24.0473061760841	8.902759526938238	58.01576872536137
14	12.319316688567675	68.69250985545335	6.176084099868594	12.812089356110382
15	52.214144084600136	16.986120290812952	16.886979510905487	13.912756113681427
16	8.053911900065746	58.71137409598948	22.386587771203157	10.848126232741617
17	10.585141354372125	18.277449046679816	52.85996055226825	18.277449046679816
18	14.5203679369251	15.144546649145859	9.494086727989487	60.84099868593955
19	9.004272099901414	12.98061123890897	19.98028261583963	58.03483404534998
20	12.598684210526315	14.473684210526317	60.065789473684205	12.861842105263158
21	15.013140604467806	13.469119579500658	14.88173455978975	56.63600525624178
22	12.582128777923785	20.203679369250985	56.27463863337714	10.939553219448095
23	16.885676741130094	18.462549277266753	53.41655716162943	11.235216819973719
24	8.705650459921156	25.689881734559787	13.042049934296978	52.56241787122208
25	12.910643889618923	22.043363994743757	56.01182654402103	9.034165571616294
26	59.612224778179424	22.477817942819588	8.938547486033519	8.971409792967467
27	19.224449556358856	61.189615511008874	10.647387446598751	8.938547486033519
28	40.73587385019711	44.38239159001314	8.015768725361367	6.865965834428383
29	36.2828947368421	45.493421052631575	13.322368421052634	4.901315789473684
30	31.514952349654944	26.19125862635557	35.88563917186987	6.408149852119618
31	36.80578376602037	37.463029904699304	18.731514952349652	6.99967137693066
32	36.563731931668855	41.4257555847569	15.045992115637318	6.964520367936925
33	44.53947368421053	34.60526315789474	11.80921052631579	9.046052631578947
34	18.334430546412115	16.589861751152075	51.61290322580645	13.462804476629362
35	14.168310322156477	20.677186061801446	57.13346482577252	8.021038790269559
36	28.56673241288626	18.704799474030242	38.00131492439185	14.72715318869165
37	34.396580072344626	16.836566918776718	32.98257152252548	15.784281486353175
38	37.10526315789473	15.756578947368421	33.81578947368421	13.322368421052634
39	32.42444152431012	21.386333771353485	16.130091984231274	30.059132720105126
40	27.636363636363637	19.074380165289256	34.01652892561983	19.272727272727273
41	24.776119402985074	14.72636815920398	30.514096185737976	29.98341625207297
42	19.27512355848435	35.617792421746294	22.899505766062603	22.207578253706757
43	13.175230566534916	39.064558629776016	28.49143610013175	19.268774703557312
44	12.335092348284961	38.39050131926121	34.9934036939314	14.281002638522427
45	28.218636812644053	23.147843266381297	33.35528482054659	15.278235100428054
46	11.787948633519921	36.3516628251564	21.63319064866645	30.227197892657227
47	10.575016523463319	35.22802379378718	13.846662260409781	40.35029742233972
48	33.04862023653088	23.587385019710908	17.772667542706966	25.59132720105125
49	8.599670510708401	36.11202635914333	20.922570016474467	34.365733113673805
50	8.092105263157896	43.223684210526315	19.67105263157895	29.013157894736842
51	14.703947368421053	36.875	20.592105263157894	27.828947368421055
52	17.63157894736842	55.85526315789474	15.69078947368421	10.822368421052632
53	10.253874052093636	53.93999340586878	19.485657764589515	16.320474777448073
54	11.19157340355497	30.4805793285056	31.36932192231731	26.95852534562212
55	16.74909330695681	22.45301681503462	37.817342565117045	22.980547312891527
56	12.146148782093483	26.46477946017117	37.656352863726134	23.732718894009217
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	2.0
11	3.0
12	15.0
13	27.0
14	27.0
15	21.0
16	15.0
17	15.0
18	15.0
19	16.5
20	18.0
21	17.0
22	19.0
23	22.0
24	19.5
25	17.0
26	19.5
27	22.0
28	26.0
29	27.0
30	24.0
31	23.0
32	22.0
33	26.0
34	30.0
35	35.5
36	41.0
37	36.0
38	39.0
39	47.0
40	55.0
41	63.0
42	76.0
43	89.0
44	95.5
45	102.0
46	178.5
47	350.5
48	446.0
49	454.0
50	462.0
51	374.5
52	287.0
53	242.5
54	195.0
55	192.0
56	171.0
57	150.0
58	127.5
59	105.0
60	94.0
61	83.0
62	64.5
63	44.0
64	42.0
65	38.5
66	35.0
67	31.5
68	28.0
69	26.0
70	24.0
71	17.5
72	9.5
73	8.0
74	7.0
75	6.0
76	3.5
77	1.0
78	1.5
79	1.5
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5913272010512484
16	0.0657030223390276
17	0.0657030223390276
18	0.0
19	0.0328515111695138
20	0.1314060446780552
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0328515111695138
27	0.0328515111695138
28	0.0
29	0.1314060446780552
30	0.0328515111695138
31	0.0328515111695138
32	0.0
33	0.1314060446780552
34	0.19710906701708278
35	0.0657030223390276
36	0.0657030223390276
37	0.09855453350854139
38	0.1314060446780552
39	0.0
40	0.6241787122207622
41	0.9526938239159002
42	0.2956636005256242
43	0.2628120893561104
44	0.39421813403416556
45	0.22996057818659657
46	0.22996057818659657
47	0.5913272010512484
48	0.0
49	0.2956636005256242
50	0.1314060446780552
51	0.1314060446780552
52	0.1314060446780552
53	0.36136662286465177
54	0.19710906701708278
55	0.36136662286465177
56	0.19710906701708278
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	3044.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.54796320630749
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.2245922208281	73.22601839684626
2	4.1405269761606025	6.504599211563732
3	1.0037641154328731	2.3653088042049935
4	0.5018820577164366	1.5768725361366622
5	0.1672940192388122	0.657030223390276
6	0.0836470096194061	0.39421813403416556
7	0.1672940192388122	0.9198423127463863
8	0.04182350480970305	0.2628120893561104
9	0.0836470096194061	0.5913272010512484
>10	0.5018820577164366	6.2089356110381075
>50	0.0	0.0
>100	0.0836470096194061	7.2930354796320636
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	112	3.679369250985545	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	110	3.613666228646518	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	28	0.9198423127463863	No Hit
ATGGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	25	0.821287779237845	No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19	0.6241787122207622	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATATCGTATGCCGT	18	0.5913272010512484	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT	16	0.5256241787122208	Illumina Single End Adapter 2 (95% over 24bp)
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	13	0.42706964520367935	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	12	0.39421813403416556	No Hit
ATGGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGCTGTAGCGCCGGAAGA	12	0.39421813403416556	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCTAATCCGCCCAATCT	12	0.39421813403416556	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	12	0.39421813403416556	No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	11	0.36136662286465177	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGAGCTCGTATGCCGT	11	0.36136662286465177	Illumina Single End Adapter 1 (95% over 23bp)
ATGGCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG	9	0.2956636005256242	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGATATCGTATGCCGT	9	0.2956636005256242	No Hit
ATGGCGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC	8	0.2628120893561104	No Hit
ATGGCGGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	7	0.22996057818659657	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTTAATCCGCCCAATCT	7	0.22996057818659657	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	7	0.22996057818659657	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATTCGCCCAATCT	7	0.22996057818659657	No Hit
ATGGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCCTTGTAGCGCCGGAAGA	6	0.19710906701708278	No Hit
ATGGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAAA	6	0.19710906701708278	No Hit
ATGGCGGGGTCACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCGGAAG	5	0.164257555847569	Illumina Single End Adapter 2 (100% over 34bp)
ATGGCGGGGTCACAGATCCTCTCCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	5	0.164257555847569	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGATCTCGTATGCCGT	5	0.164257555847569	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATCTCGTATGCCGT	5	0.164257555847569	Illumina Single End Adapter 1 (95% over 23bp)
ATGGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	4	0.1314060446780552	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGTACAAAAACTC	4	0.1314060446780552	No Hit
ATGGCGGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	4	0.1314060446780552	No Hit
ATGGCGAGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	4	0.1314060446780552	No Hit
ATGGCGGGGTCCCCCCTCCTCTTCTGAGATGAGTTTTTGTTCCCTCCGCCCAATCT	4	0.1314060446780552	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCTAATCCGCCCAAAAA	4	0.1314060446780552	No Hit
ATGGCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	4	0.1314060446780552	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCCATTGAACAAAAACTC	4	0.1314060446780552	No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA	4	0.1314060446780552	No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA	4	0.1314060446780552	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGCCGAGCTCGTATGCCGT	4	0.1314060446780552	No Hit
ATGGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTT	4	0.1314060446780552	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.36136662286465177	0.0	0.0	0.0	0.0
15	0.36136662286465177	0.0	0.0	0.0	0.0
16	0.36136662286465177	0.0	0.0	0.0	0.0
17	0.36136662286465177	0.0	0.0	0.0	0.0
18	0.36136662286465177	0.0	0.0	0.0	0.0
19	0.36136662286465177	0.0	0.0	0.0	0.0
20	0.39421813403416556	0.0	0.0	0.0	0.0
21	0.39421813403416556	0.0	0.0	0.0	0.0
22	0.39421813403416556	0.0	0.0	0.0	0.0
23	0.39421813403416556	0.0	0.0	0.0	0.0
24	0.39421813403416556	0.0	0.0	0.0	0.0
25	0.39421813403416556	0.0	0.0	0.0	0.0
26	0.39421813403416556	0.0	0.0	0.0	0.0
27	0.39421813403416556	0.0	0.0	0.0	0.0
28	0.45992115637319314	0.0	0.0	0.0	0.0
29	0.45992115637319314	0.0	0.0	0.0	0.0
30	0.45992115637319314	0.0	0.0	0.0	0.0
31	0.492772667542707	0.0	0.0	0.0	0.0
32	0.5256241787122208	0.0	0.0	0.0	0.0
33	0.5584756898817346	0.0	0.0	0.0	0.0
34	0.5584756898817346	0.0	0.0	0.0	0.0
35	0.5584756898817346	0.0	0.0	0.0	0.0
36	0.5584756898817346	0.0	0.0	0.0	0.0
37	0.5584756898817346	0.0	0.0	0.0	0.0
38	0.5584756898817346	0.0	0.0	0.0	0.0
39	0.5584756898817346	0.0	0.0	0.0	0.0
40	0.5584756898817346	0.0	0.0	0.0	0.0
41	0.5584756898817346	0.0	0.0	0.0	0.0
42	0.5584756898817346	0.0	0.0	0.0	0.0
43	0.5584756898817346	0.0	0.0	0.0	0.0
44	0.5913272010512484	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGGT	135	0.0	50.000004	4
GCGGGGC	20	3.5472712E-4	50.0	4
GCGGGGA	55	0.0	50.0	4
CCAATCT	25	1.6275591E-5	50.0	50
CGGGGTC	130	0.0	50.0	5
CGGGGGG	40	1.4606485E-9	50.0	5
AGGATCG	20	3.5472712E-4	50.0	32
CGGGGAG	20	3.5472712E-4	50.0	5
GAAGCAG	20	3.5472712E-4	50.0	9
GAGCTGT	20	3.5472712E-4	50.0	9
GCGGATT	20	3.5472712E-4	50.0	38
GATGAGT	50	1.8189894E-12	50.0	28
GATGAGG	25	1.6275591E-5	50.0	28
GGGAGCT	20	3.5472712E-4	50.0	7
ACCGACC	20	3.5472712E-4	50.0	18
GGAAGCA	20	3.5472712E-4	50.0	8
ATTGAAC	25	1.6275591E-5	50.0	42
TGAGGAT	20	3.5472712E-4	50.0	30
GGGGTCC	20	3.5472712E-4	50.0	6
GGGAAGC	20	3.5472712E-4	50.0	7
>>END_MODULE